FastQCFastQC Report
Fri 27 May 2016
SRR939474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327383
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173490.5214007524832579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148750.44704802543019545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112620.33846419242990666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47200.14185322218692586No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37110.11152909057959365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35020.10524787798699459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140550.031.0350781
GTATCAA207000.029.349981
GTACATG139750.024.8954721
TCAACGC255950.023.4320264
TACATGG145900.023.3832342
CAACGCA257600.023.3359585
ATCAACG260050.023.1142023
TATCAAC261050.023.0839982
AACGCAG263600.022.8407616
ACATGGG145300.022.3984323
GAGTACT147900.022.05059412-13
AGTACTT160150.020.85230612-13
CCCTATA9900.020.6093462
GTACTTT158400.020.60386814-15
ACGCAGA293350.020.4112457
CGCAGAG295500.020.1504348
AGAGTAC238250.019.87751210-11
CATGGGA70600.019.4227664
CATGGGG66100.019.381184
GCAGAGT305850.018.8020239
Produced by FastQC (version 0.11.5)