##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939474_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3327383 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.891453734060672 31.0 31.0 31.0 28.0 34.0 2 31.14271185493224 31.0 31.0 34.0 28.0 34.0 3 31.14425601140596 31.0 31.0 34.0 28.0 34.0 4 35.06546015291897 35.0 35.0 37.0 32.0 37.0 5 34.63587119366782 35.0 35.0 37.0 31.0 37.0 6 34.688030503251355 35.0 35.0 37.0 31.0 37.0 7 34.525228685726894 35.0 35.0 37.0 30.0 37.0 8 34.41897701587103 35.0 35.0 37.0 30.0 37.0 9 35.91550596970652 37.0 35.0 39.0 30.0 39.0 10-11 35.71421143883947 37.0 35.0 39.0 30.0 39.0 12-13 35.70930397853208 37.0 35.0 39.0 30.0 39.0 14-15 36.525834116481334 38.0 35.0 40.0 30.0 41.0 16-17 36.4391596939697 38.0 35.0 40.0 30.0 41.0 18-19 36.46079546598634 38.0 35.0 40.0 30.0 41.0 20-21 36.327146439108446 38.0 34.5 40.0 29.5 41.0 22-23 36.15936082500872 38.0 34.0 40.0 29.0 41.0 24-25 35.99252280245466 38.0 34.0 40.0 29.0 41.0 26-27 35.504978987991464 38.0 34.0 40.0 27.0 41.0 28-29 35.30345439644309 38.0 33.5 40.0 27.0 41.0 30-31 34.994926793819644 37.5 33.0 40.0 27.0 41.0 32-33 34.72783656104512 37.0 33.0 40.0 25.5 41.0 34-35 34.96777272108441 37.5 33.0 40.0 26.0 41.0 36-37 34.794259632870634 38.0 33.0 40.0 25.0 41.0 38-39 34.59621916082399 37.0 33.0 40.0 25.0 41.0 40-41 34.43570953509109 37.0 33.0 40.0 24.5 41.0 42-43 34.266724930673746 37.0 33.0 40.0 24.0 41.0 44-45 33.87100853734 37.0 32.5 40.0 23.5 41.0 46-47 33.45185495628246 37.0 32.0 40.0 23.0 41.0 48-49 33.05109811524552 37.0 31.0 39.5 20.5 41.0 50-51 32.80768610045793 36.0 31.0 39.0 19.5 40.0 52-53 32.686104815706514 36.0 31.0 39.0 19.5 40.0 54-55 32.3348307062938 36.0 30.5 39.0 18.0 40.0 56-57 31.82165969472105 35.0 30.0 39.0 15.0 40.0 58-59 31.35135810936102 35.0 29.0 38.5 13.0 40.0 60-61 30.972842921899883 35.0 29.0 38.0 9.5 40.0 62-63 30.795091367600303 34.0 28.5 38.0 9.0 40.0 64-65 30.326550024448643 34.0 28.0 38.0 7.5 40.0 66-67 29.46139142984141 33.5 27.0 37.0 4.5 39.5 68-69 26.069714246902144 31.0 18.5 35.5 2.0 39.0 70-71 24.780488900736707 29.5 12.5 35.0 2.0 38.0 72-73 25.761186193473968 30.5 19.0 35.0 2.0 37.5 74-75 26.488621688576277 31.0 22.0 35.0 2.0 37.0 76-77 26.13606488943413 30.5 22.5 34.0 2.0 36.0 78-79 27.23982180590572 32.0 25.5 35.0 2.0 36.0 80-81 27.2595907955291 32.0 26.0 35.0 2.0 36.0 82-83 26.950369855228566 32.0 25.0 35.0 2.0 36.0 84-85 26.59220925273706 32.0 25.0 34.5 2.0 35.5 86-87 26.174164801587313 32.0 24.0 34.0 2.0 35.0 88-89 25.698234618617693 31.0 22.5 34.0 2.0 35.0 90-91 25.111759151260916 31.0 20.0 34.0 2.0 35.0 92-93 24.563670307866573 31.0 18.5 34.0 2.0 35.0 94-95 23.728108847102966 30.5 9.0 34.0 2.0 35.0 96-97 23.06356602170535 30.0 2.0 34.0 2.0 35.0 98-99 22.220140422668507 30.0 2.0 34.0 2.0 35.0 100-101 20.789087700454083 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2168701528462016 1101 2 0.1740351056003 1101 3 0.29346254804534766 1101 4 0.256872903467638 1101 5 0.5491044676132972 1101 6 0.322918989121348 1101 7 0.46894716051516383 1101 8 0.6532773905058278 1101 9 0.714278985143423 1101 10-11 0.7175129537616272 1101 12-13 0.6890020140182003 1101 14-15 0.7473122860584738 1101 16-17 0.6917005242094874 1101 18-19 0.5058037252061851 1101 20-21 0.59458191462695 1101 22-23 0.594733755488889 1101 24-25 0.7086265318995828 1101 26-27 0.782186715878197 1101 28-29 0.7928194517694962 1101 30-31 0.5091591267456721 1101 32-33 0.9213500850333531 1101 34-35 0.8154209000781307 1101 36-37 0.9974097971116151 1101 38-39 1.0253553359622956 1101 40-41 0.886494020838569 1101 42-43 0.5984096142772373 1101 44-45 0.8062072734178329 1101 46-47 0.736952479922401 1101 48-49 0.8651605440254997 1101 50-51 0.6401527320315452 1101 52-53 0.38560227478242837 1101 54-55 0.3110652030805312 1101 56-57 0.4795897427313953 1101 58-59 0.33787977306866424 1101 60-61 0.38862849345822426 1101 62-63 0.032215339579821034 1101 64-65 -0.09521338246904065 1101 66-67 -0.383643891476531 1101 68-69 -6.999965240113358 1101 70-71 -7.732368031226908 1101 72-73 -5.030538342029249 1101 74-75 -3.084303550426153 1101 76-77 -0.5243220105551032 1101 78-79 -0.1653189576972025 1101 80-81 -0.24894960935680643 1101 82-83 -0.5547219802198953 1101 84-85 -0.7200530741851843 1101 86-87 -0.7460145285084607 1101 88-89 -0.7356634740114778 1101 90-91 -0.7241323327817177 1101 92-93 -0.7317285211796651 1101 94-95 -0.7239159127786721 1101 96-97 -0.8882178469706723 1101 98-99 -1.1023605405729882 1101 100-101 -0.8400755405311919 1102 1 0.3314202747817667 1102 2 0.3495035571318539 1102 3 0.28861849721747035 1102 4 0.3163264962254999 1102 5 0.41755713169779796 1102 6 0.47641367001256896 1102 7 0.4676923189558977 1102 8 0.6187236444791182 1102 9 0.5415082164585954 1102 10-11 0.5731472829650102 1102 12-13 0.7170058193139113 1102 14-15 0.8132029819594777 1102 16-17 0.654455399744613 1102 18-19 0.5738372404990955 1102 20-21 0.7475372854545199 1102 22-23 0.7621726178262804 1102 24-25 0.5588016963049682 1102 26-27 0.6437022277433257 1102 28-29 0.5107666306410437 1102 30-31 0.46830033039694996 1102 32-33 0.6671155232841244 1102 34-35 0.3835086257344358 1102 36-37 0.6179943705636362 1102 38-39 0.6913853706176667 1102 40-41 0.6008503293997265 1102 42-43 0.3692977808646347 1102 44-45 0.34280496909988045 1102 46-47 0.30496406191368663 1102 48-49 0.40258275819418543 1102 50-51 0.38413859052589316 1102 52-53 0.13971977083745202 1102 54-55 -0.10210965176706566 1102 56-57 0.08777588511287959 1102 58-59 -0.058201225368446075 1102 60-61 0.2824500220696713 1102 62-63 -0.017281289260392185 1102 64-65 -0.13019937686680194 1102 66-67 -0.17737980093465566 1102 68-69 -6.8947206139610095 1102 70-71 -7.8575572389854855 1102 72-73 -1.6265772481577372 1102 74-75 -0.100844732936892 1102 76-77 0.4064771392935178 1102 78-79 -0.0025005095632302243 1102 80-81 -0.09035722900843979 1102 82-83 -0.11786852185541719 1102 84-85 -0.49989014108741614 1102 86-87 -0.4422100633261614 1102 88-89 -0.5248329531238802 1102 90-91 -0.5400183475649918 1102 92-93 -0.36228923603164276 1102 94-95 -0.3828247970871139 1102 96-97 -0.5408588656422957 1102 98-99 -0.7301675124183298 1102 100-101 -0.8201100826499292 1103 1 0.32985581444775747 1103 2 0.2859664731985241 1103 3 0.328732151762825 1103 4 0.3327203368112066 1103 5 0.4499895044847051 1103 6 0.39351603082712927 1103 7 0.35122178913812974 1103 8 0.4158299776855543 1103 9 0.4762603557571339 1103 10-11 0.567648830542673 1103 12-13 0.48870550648804567 1103 14-15 0.6430400587204304 1103 16-17 0.6724996588107643 1103 18-19 0.6425696612857408 1103 20-21 0.5489930567330319 1103 22-23 0.6245308944702685 1103 24-25 0.5506047760121149 1103 26-27 0.6025953648846283 1103 28-29 0.6325336829607551 1103 30-31 0.47409891909438784 1103 32-33 0.5556835311847692 1103 34-35 0.7360908459704589 1103 36-37 0.8309586024851185 1103 38-39 0.8838867085413256 1103 40-41 0.9381130203641845 1103 42-43 0.6576846035580175 1103 44-45 0.7451445975708921 1103 46-47 0.7890527565967531 1103 48-49 0.9490394398124948 1103 50-51 0.8295951121450784 1103 52-53 0.7767463251737041 1103 54-55 0.6181079470323851 1103 56-57 0.7510277292406364 1103 58-59 0.9569706633383781 1103 60-61 0.8575787661869434 1103 62-63 0.4195849349259575 1103 64-65 0.5741129855477531 1103 66-67 0.36322698192447334 1103 68-69 -6.707373675185497 1103 70-71 -7.539029451552587 1103 72-73 -0.8396876861691105 1103 74-75 0.5587882621914098 1103 76-77 0.7195819497497347 1103 78-79 0.4961559392287782 1103 80-81 0.3084395776298692 1103 82-83 0.1761853592266185 1103 84-85 0.051420693059707645 1103 86-87 -0.008471325332990887 1103 88-89 0.014427740610223339 1103 90-91 -0.046508022104720936 1103 92-93 0.09975235270442084 1103 94-95 0.3621920028201977 1103 96-97 0.27403462460052097 1103 98-99 0.24580889598768962 1103 100-101 0.3671500152253948 1104 1 0.24875674219973476 1104 2 0.2849631618564139 1104 3 0.20776987789891166 1104 4 0.2104921926440042 1104 5 0.45898453150594065 1104 6 0.2946302440480011 1104 7 0.3896536115461302 1104 8 0.43918863133499997 1104 9 0.4745071253870705 1104 10-11 0.43143027323323935 1104 12-13 0.34191278532959046 1104 14-15 0.4937764169308849 1104 16-17 0.4459547077562931 1104 18-19 0.4109648665764425 1104 20-21 0.4302996735098574 1104 22-23 0.42975754200046623 1104 24-25 0.4255419553795363 1104 26-27 0.6794516254571974 1104 28-29 0.6092491991259195 1104 30-31 0.48032500032950765 1104 32-33 0.5787738421552646 1104 34-35 0.35737464361628923 1104 36-37 0.6570870603844554 1104 38-39 0.5526391875373307 1104 40-41 0.5864858968667548 1104 42-43 0.5622074635751702 1104 44-45 0.5151556790286307 1104 46-47 0.4975255902925326 1104 48-49 0.5066100935632107 1104 50-51 0.40893553406110783 1104 52-53 0.16273186049570398 1104 54-55 0.1715210534752032 1104 56-57 0.2812142981530137 1104 58-59 0.41323931855633234 1104 60-61 0.5307563349069682 1104 62-63 0.3430265540060873 1104 64-65 0.34062844618922483 1104 66-67 0.1995699219038869 1104 68-69 -6.548735354410471 1104 70-71 -4.319404591489459 1104 72-73 0.18215396014905139 1104 74-75 0.9015859766989536 1104 76-77 1.1289964527239462 1104 78-79 0.6620608284272933 1104 80-81 0.3093010605721922 1104 82-83 0.24866490960112841 1104 84-85 0.29682507460058005 1104 86-87 0.17587034644808242 1104 88-89 0.1826620857958865 1104 90-91 0.08563516033978402 1104 92-93 0.17766485109297747 1104 94-95 0.4270570523157282 1104 96-97 0.1624896255302808 1104 98-99 0.2545170287824341 1104 100-101 0.3733403028864153 1105 1 0.09530681052814316 1105 2 0.23061509608706032 1105 3 0.24683182028254436 1105 4 0.20037218997469353 1105 5 0.24557664138846746 1105 6 0.23413668374636387 1105 7 0.2538686762912832 1105 8 0.4261963694181503 1105 9 0.332441344426762 1105 10-11 0.457571618737056 1105 12-13 0.3954155228179488 1105 14-15 0.4156609462772778 1105 16-17 0.3746440789898884 1105 18-19 0.52102887186863 1105 20-21 0.537732382361213 1105 22-23 0.589101125078237 1105 24-25 0.5870394064833775 1105 26-27 0.6755710507442174 1105 28-29 0.5413211165863672 1105 30-31 0.6007993677477401 1105 32-33 0.6199508460924932 1105 34-35 0.558464343870746 1105 36-37 0.6036290772765085 1105 38-39 0.682093880436689 1105 40-41 0.7304293308116101 1105 42-43 0.6395583379176415 1105 44-45 0.646380890034294 1105 46-47 0.8510954956192407 1105 48-49 1.0034196212412922 1105 50-51 0.7596667640594532 1105 52-53 0.6375762719284523 1105 54-55 0.7757314469365681 1105 56-57 0.783085858158703 1105 58-59 0.9710017010851537 1105 60-61 0.9952481224418932 1105 62-63 0.6841100597320349 1105 64-65 0.6768176941125326 1105 66-67 0.8142156296904233 1105 68-69 -2.7513089851585413 1105 70-71 -2.7687522075389417 1105 72-73 1.1696003927099312 1105 74-75 1.420897020368205 1105 76-77 1.3883742000045949 1105 78-79 1.0239826119872788 1105 80-81 0.7585222473103883 1105 82-83 0.624008002339167 1105 84-85 0.6323252356293061 1105 86-87 0.7513376198980701 1105 88-89 0.6969986474408749 1105 90-91 0.6244842836761855 1105 92-93 0.6847650573838351 1105 94-95 0.8010206656262753 1105 96-97 0.748546858911677 1105 98-99 0.8764170081823615 1105 100-101 0.9703057818090635 1106 1 0.08313106870324205 1106 2 0.16500748632154583 1106 3 0.12247390156043281 1106 4 0.18018127307927045 1106 5 0.2029573037036556 1106 6 0.12557013997982835 1106 7 0.1615444012972418 1106 8 0.24168280969701783 1106 9 0.21397531941480707 1106 10-11 0.30389960559780604 1106 12-13 0.1477933551638415 1106 14-15 0.10947311236650137 1106 16-17 0.24402520053524057 1106 18-19 0.17959951068117874 1106 20-21 0.23032049532013588 1106 22-23 0.32824464620355087 1106 24-25 0.37108664283919524 1106 26-27 0.5457064651409453 1106 28-29 0.38690358569436256 1106 30-31 0.2982304576296144 1106 32-33 0.4826895836190559 1106 34-35 0.41698233209163504 1106 36-37 0.5231625930343995 1106 38-39 0.5954727614068673 1106 40-41 0.6873135419229328 1106 42-43 0.45215828263452096 1106 44-45 0.5804643236718832 1106 46-47 0.6373036000078187 1106 48-49 0.726310782230712 1106 50-51 0.7037169546984714 1106 52-53 0.4707524334955764 1106 54-55 0.47987775415569445 1106 56-57 0.6529499026308976 1106 58-59 0.8220814769691351 1106 60-61 0.9476914023982523 1106 62-63 0.5313333991543132 1106 64-65 0.7170013569264455 1106 66-67 0.8349952825767737 1106 68-69 -2.705610245993963 1106 70-71 -2.8372825832740425 1106 72-73 1.0740778136499465 1106 74-75 1.4099201763909228 1106 76-77 1.453048870536204 1106 78-79 1.0982108786274232 1106 80-81 0.853808118085361 1106 82-83 0.6903671261748201 1106 84-85 0.5317756950788457 1106 86-87 0.6423429244451846 1106 88-89 0.7416537348774206 1106 90-91 0.7620191098487936 1106 92-93 0.7250627964796799 1106 94-95 1.0583714926061916 1106 96-97 1.0037434723086243 1106 98-99 0.9282133439936864 1106 100-101 0.8564411260049916 1107 1 -0.00465522584101663 1107 2 0.052719040508304005 1107 3 0.09540780448967467 1107 4 -0.12943761223478845 1107 5 0.25197123536743504 1107 6 0.20845352820936824 1107 7 0.017235291074321424 1107 8 0.02869969123584326 1107 9 0.291284972231324 1107 10-11 0.008444782246414206 1107 12-13 0.18257925995776958 1107 14-15 0.1119363344554074 1107 16-17 0.037956405780825264 1107 18-19 0.1471159604377803 1107 20-21 0.1307930102796675 1107 22-23 0.310477686332554 1107 24-25 0.14811567541774195 1107 26-27 0.46922201312226264 1107 28-29 0.411353006036272 1107 30-31 0.2956932593995347 1107 32-33 0.3073780508303585 1107 34-35 0.19996162947114016 1107 36-37 0.37893525395925565 1107 38-39 0.36972684805371614 1107 40-41 0.245640978053558 1107 42-43 0.12480415242733756 1107 44-45 0.2541865921716422 1107 46-47 0.3830449280629651 1107 48-49 0.5597610661370851 1107 50-51 0.32773061888652677 1107 52-53 0.15745858467284535 1107 54-55 0.2881413782223916 1107 56-57 0.6104921828995948 1107 58-59 0.6857999243872293 1107 60-61 0.5765738124308832 1107 62-63 0.22925602989262117 1107 64-65 0.16656789342159684 1107 66-67 0.36509600628964023 1107 68-69 -2.7928666329319753 1107 70-71 -2.733077024364423 1107 72-73 1.0284337703341642 1107 74-75 1.1428958055320173 1107 76-77 0.9005635625952308 1107 78-79 0.6856359281104325 1107 80-81 0.5277686692381849 1107 82-83 0.5849464632935728 1107 84-85 0.33331001221334233 1107 86-87 0.4375264509978791 1107 88-89 0.4280289803819173 1107 90-91 0.31796478935174477 1107 92-93 0.29164292838341765 1107 94-95 0.6728425402703628 1107 96-97 0.6750815386884561 1107 98-99 0.8280532406716397 1107 100-101 1.0427844272667137 1108 1 -0.054343027172009783 1108 2 0.034964974021843176 1108 3 0.017431800316487056 1108 4 0.04406533586909944 1108 5 0.005141593091778418 1108 6 -0.0541131400380479 1108 7 0.08822073234145478 1108 8 0.2524791129964896 1108 9 0.24949145015611407 1108 10-11 0.26427855028675395 1108 12-13 0.18376948774424307 1108 14-15 0.12612414369016278 1108 16-17 0.295585750874956 1108 18-19 0.1347440409297107 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0.45719507315734376 2312 28-29 0.4086554413377357 2312 30-31 0.17027077374670085 2312 32-33 0.7496090724279227 2312 34-35 0.5462320599356971 2312 36-37 0.6497771526419882 2312 38-39 0.6333347020743929 2312 40-41 0.41461997518907623 2312 42-43 0.17159663143934267 2312 44-45 0.7285028344529181 2312 46-47 0.6769509256246877 2312 48-49 0.8364696922781647 2312 50-51 1.0077534157663308 2312 52-53 0.9013547696645645 2312 54-55 1.094367007862303 2312 56-57 0.9586485100249114 2312 58-59 0.9525799146737732 2312 60-61 1.1184997523065938 2312 62-63 1.0519117672725642 2312 64-65 1.1639971983149984 2312 66-67 1.5838822211155446 2312 68-69 4.2845334324851585 2312 70-71 5.045325707296474 2312 72-73 3.11304508353356 2312 74-75 2.5437881547009233 2312 76-77 2.4799652931757556 2312 78-79 1.754182577464686 2312 80-81 1.5795723980147827 2312 82-83 1.5018312201103363 2312 84-85 1.4973113602027546 2312 86-87 1.505899249364436 2312 88-89 1.4612730754944643 2312 90-91 1.6300191846892886 2312 92-93 1.7191674845876364 2312 94-95 1.8156316889555804 2312 96-97 2.0720029687466344 2312 98-99 2.3941815375464834 2312 100-101 2.305456419813126 2313 1 -0.29997360109429394 2313 2 -0.15167336386517505 2313 3 -0.14139234215169338 2313 4 0.03028535447861458 2313 5 0.0029007731994212804 2313 6 -0.05138675159820849 2313 7 -2.852656263670962 2313 8 -3.0183519750757775 2313 9 -0.8959884396079829 2313 10-11 -0.25834144337321874 2313 12-13 -0.06512372191517812 2313 14-15 -0.6352674227342305 2313 16-17 -0.36677267924639523 2313 18-19 0.15466883307281165 2313 20-21 -2.653955197574568 2313 22-23 -0.7204489781212757 2313 24-25 -0.011801838812914411 2313 26-27 -1.5353755405430292 2313 28-29 -1.589631770013625 2313 30-31 -0.7740134083240093 2313 32-33 -0.5574194007804678 2313 34-35 -1.173059529491077 2313 36-37 -1.8346950611710682 2313 38-39 -1.6299881425582683 2313 40-41 -0.6053412714052087 2313 42-43 0.30530937157196547 2313 44-45 0.10433085557124855 2313 46-47 0.04696376999248031 2313 48-49 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1.3618322690238607 2314 72-73 1.1201108856203064 2314 74-75 0.7685484009492072 2314 76-77 0.7730630281495863 2314 78-79 -1.3802424768054742 2314 80-81 -0.9712223968193001 2314 82-83 0.1757856175271293 2314 84-85 0.3791019612247375 2314 86-87 -0.0017090680109852485 2314 88-89 -0.009503322507072198 2314 90-91 0.11787475390432789 2314 92-93 -0.3962980389880535 2314 94-95 0.18021152651398253 2314 96-97 0.3646588543295657 2314 98-99 0.24412165965713584 2314 100-101 0.3829368064198526 2315 1 -0.3431874560474242 2315 2 -0.42157877354800277 2315 3 -0.44004909853016017 2315 4 -0.15567679204011142 2315 5 -0.19767541824030843 2315 6 -0.29065094489309473 2315 7 -1.0782512211344368 2315 8 -2.7363558259933782 2315 9 -0.7058563310765464 2315 10-11 -1.4167310852575028 2315 12-13 -0.7311089975817424 2315 14-15 -0.5747741752959854 2315 16-17 -0.27025168437613445 2315 18-19 -0.2385032895634822 2315 20-21 -0.7902195434121708 2315 22-23 -0.341166479700469 2315 24-25 -5.186664899342944 2315 26-27 -0.9174844178122967 2315 28-29 -0.9217434202151935 2315 30-31 -1.116108620884674 2315 32-33 -0.18507533232160966 2315 34-35 -2.110339447638644 2315 36-37 -3.3967181372496427 2315 38-39 -0.9777814480583231 2315 40-41 0.0691577238626877 2315 42-43 -1.457841841519901 2315 44-45 -4.960470308833962 2315 46-47 -3.183826065506608 2315 48-49 -2.4206562320747835 2315 50-51 -1.149095608409315 2315 52-53 -0.24119661430795247 2315 54-55 -1.3063543843401106 2315 56-57 -1.3641756956624427 2315 58-59 -0.6635235459305626 2315 60-61 -1.294288076816649 2315 62-63 -0.29236839668112324 2315 64-65 0.08150431927021984 2315 66-67 0.39101875047732904 2315 68-69 3.2307743405456435 2315 70-71 3.676829263559757 2315 72-73 -2.174553383910574 2315 74-75 -1.897853286805347 2315 76-77 -0.3315365245202422 2315 78-79 -0.1720351178291324 2315 80-81 -0.28564328622718094 2315 82-83 -1.0104805284027627 2315 84-85 -0.3083504690870491 2315 86-87 -0.01243175371844174 2315 88-89 -0.07682048106319073 2315 90-91 0.10444683500428198 2315 92-93 -1.2030953475359532 2315 94-95 -0.09739176509705416 2315 96-97 -0.2932202635443204 2315 98-99 -2.5640230281677105 2315 100-101 -4.070121100285444 2316 1 -0.5235868527376759 2316 2 -0.535735079436833 2316 3 -0.9549412927462626 2316 4 -0.42227217931898764 2316 5 -0.24011262055324067 2316 6 -0.5122602504840117 2316 7 -0.7042451032278336 2316 8 -1.0123581144844138 2316 9 -5.334699582310783 2316 10-11 -1.5606317458879104 2316 12-13 -0.7234034266125775 2316 14-15 -0.5275790603003969 2316 16-17 -0.481179310372859 2316 18-19 -0.4568547604392066 2316 20-21 -3.9175497785487963 2316 22-23 -1.1710145562475915 2316 24-25 -0.6241283457383773 2316 26-27 -0.7323518331383312 2316 28-29 -0.17186748401277185 2316 30-31 -0.17899572330304636 2316 32-33 -1.4260121044247853 2316 34-35 -0.5968010153633827 2316 36-37 -0.3553519547038704 2316 38-39 -5.392337901184419 2316 40-41 -5.016712239394373 2316 42-43 -3.715102875949821 2316 44-45 -2.521686631253786 2316 46-47 -2.701087292315009 2316 48-49 -3.0107938815863946 2316 50-51 -1.3034710740295878 2316 52-53 -0.8453160472032906 2316 54-55 -0.13393916871973488 2316 56-57 -3.8308144900292227 2316 58-59 -0.8543545301917028 2316 60-61 -0.4448088602631053 2316 62-63 -0.08275775040187838 2316 64-65 0.07511071533984293 2316 66-67 0.10937889193240835 2316 68-69 2.77519547979745 2316 70-71 3.4855222103008607 2316 72-73 2.352299671310316 2316 74-75 0.8855166232766827 2316 76-77 -1.6424836864160817 2316 78-79 -0.013288867346659572 2316 80-81 -0.007541227276181672 2316 82-83 0.1914863925241299 2316 84-85 0.2856104994871167 2316 86-87 0.24220358542246245 2316 88-89 0.18393588066958344 2316 90-91 0.08085283379880082 2316 92-93 0.1844275939364195 2316 94-95 0.32699963135800303 2316 96-97 0.6760873080526473 2316 98-99 0.8376666238887118 2316 100-101 0.595839681871265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 26.0 4 98.0 5 261.0 6 713.0 7 1851.0 8 3803.0 9 6414.0 10 9717.0 11 14208.0 12 18746.0 13 23140.0 14 27486.0 15 34759.0 16 40899.0 17 45804.0 18 42589.0 19 38627.0 20 38083.0 21 39161.0 22 41066.0 23 45722.0 24 54389.0 25 67121.0 26 84135.0 27 103372.0 28 126694.0 29 151000.0 30 175820.0 31 203461.0 32 233907.0 33 269747.0 34 308160.0 35 343750.0 36 345363.0 37 273466.0 38 107769.0 39 6053.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.420582239197465 21.061787238545165 15.483573082303826 23.03405743995354 2 15.747711730668069 21.870774780190878 38.559374765161195 23.82213872397986 3 18.25749909763486 25.946792163293082 31.204913674986862 24.590795064085206 4 12.676700739921658 17.89441482177568 37.331421011753676 32.097463426548984 5 12.695442634647108 38.69731858340323 34.676741451164474 13.930497330785185 6 30.77580789467278 37.84193163215656 17.096408799347714 14.285851673822942 7 27.263768553244393 33.79568868386957 21.519133805756656 17.421408957129373 8 26.092097002358912 35.10762061355726 19.907957695281848 18.89232468880198 9 26.13444259347361 16.34494736554223 21.007109791689142 36.513500249295014 10-11 24.576790551222754 26.950857149040182 27.537002461019828 20.93534983871724 12-13 25.362559705329986 24.7791432486131 28.176167276204755 21.682129769852164 14-15 22.68647763121949 25.989433738166 26.36139272214831 24.962695908466202 16-17 21.178051594549082 29.23069502101812 27.338147550300285 22.253105834132512 18-19 21.158133426299372 27.51487656955322 30.047349566325458 21.27964043782195 20-21 21.892595472177383 26.661117761315722 30.068074519825338 21.378212246681553 22-23 21.85864938419239 26.357040535063863 30.15579682323487 21.628513257508878 24-25 21.74340933715431 26.880572365425447 30.04650995042445 21.329508346995787 26-27 21.479448563516044 26.83682300237717 30.225273035114185 21.458455398992598 28-29 21.297943002380723 26.89610754746383 30.42998639006813 21.375963060087315 30-31 21.47329563196476 26.81721968986382 30.030666798502242 21.678817879669182 32-33 21.39180164715998 26.997050367355413 29.787863574268336 21.823284411216267 34-35 21.84224343467403 26.900667840594643 29.709213892022067 21.54787483270925 36-37 22.047749066679582 26.492282415494305 29.73975017002961 21.720218347796504 38-39 21.56009588482269 27.035927927537646 29.728055576614597 21.67592061102507 40-41 21.445617230775156 26.910092352411795 30.154623515936684 21.48966690087636 42-43 21.474283486693036 26.897098753901673 29.42463867901004 22.203979080395257 44-45 21.531777041843007 26.943619776392136 29.48397837915881 22.04062480260605 46-47 22.121055894325146 27.207662967088197 28.87563153967093 21.795649598915723 48-49 21.818043463745422 27.391155662576388 28.673114785457383 22.117686088220804 50-51 21.863116784292615 27.39302004177689 28.638145695762606 22.105717478167893 52-53 21.97982364104882 27.534998287412225 28.51003059202009 21.975147479518863 54-55 21.95666273664688 27.44281400241695 28.441210422039624 22.159312838896554 56-57 21.93636808555923 27.72131441262802 28.215145061813757 22.12717243999899 58-59 21.853139259291993 27.23511525118899 28.48901235575471 22.422733133764304 60-61 21.89009827006926 27.590838346427393 28.62552352692583 21.89353985657752 62-63 21.731013826158982 27.505653205165963 28.86380494067891 21.899528027996137 64-65 21.726407214730255 27.515205760531476 28.52037738718493 22.23800963755334 66-67 21.398300525109175 27.63763478582285 28.456941152886372 22.507123536181602 68-69 21.547965264770987 27.13432847115803 29.07568723158711 22.242019032483874 70-71 21.279398560706557 27.133939059612906 29.36151950919769 22.22514287048284 72-73 21.65469352017444 27.392695226983076 28.358373715086614 22.594237537755866 74-75 21.611916018351536 27.964424544989146 27.923364377786612 22.5002950588727 76-77 21.015556366893883 27.71320277477708 28.731002450325395 22.540238408003642 78-79 21.566381379657354 27.624782758461073 28.25202543356632 22.556810428315252 80-81 21.721679828821113 27.84558676239853 27.808424269737486 22.62430913904287 82-83 21.833090871331944 27.77460455852606 27.79936919306512 22.592935377076877 84-85 21.41027839183309 27.79891136520838 27.808408331063028 22.982401911895504 86-87 21.631167892853156 27.77055057649223 27.84884177858978 22.749439752064838 88-89 21.631791561722782 27.71334507532743 27.6635460371371 22.991317325812687 90-91 21.48878190052647 27.679316626278045 27.858578402570046 22.973323070625444 92-93 21.602280556885063 27.796375844069775 27.636574566290705 22.964769032754454 94-95 21.202214652636783 27.283757143731506 28.0979128265875 23.416115377044207 96-97 21.396026205744135 27.10259426765131 27.950062850612767 23.55131667599179 98-99 21.31200708396899 27.52601971446207 27.445765992470278 23.71620720909867 100-101 21.074879463009623 27.282177537814245 27.840640796011527 23.802302203164604 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3654.0 1 2494.5 2 1447.0 3 1627.5 4 1532.0 5 1536.5 6 2536.0 7 4790.5 8 6845.5 9 7586.5 10 6986.5 11 5929.5 12 5510.0 13 5364.5 14 5328.5 15 5435.5 16 5605.0 17 5824.5 18 6186.0 19 6716.5 20 7384.5 21 8269.5 22 9505.0 23 11016.5 24 12474.0 25 15306.0 26 20075.5 27 24693.5 28 27470.0 29 31461.0 30 38804.5 31 48165.5 32 60503.5 33 71580.5 34 79606.5 35 88247.0 36 99699.0 37 108087.0 38 109669.0 39 108723.5 40 104628.5 41 100221.0 42 97580.0 43 99668.5 44 103366.0 45 104936.0 46 105600.0 47 107504.5 48 112270.5 49 110083.0 50 102990.5 51 99431.5 52 99984.5 53 103480.5 54 104644.0 55 99950.0 56 93213.5 57 85751.0 58 78982.5 59 72393.5 60 62410.0 61 51138.5 62 40558.5 63 30694.5 64 22783.5 65 17086.0 66 12231.0 67 8901.0 68 6576.5 69 4660.5 70 3335.0 71 2325.0 72 1662.5 73 1304.5 74 995.0 75 731.5 76 525.0 77 354.0 78 220.0 79 123.5 80 74.0 81 54.5 82 38.5 83 26.5 84 15.5 85 10.0 86 7.5 87 3.0 88 2.5 89 2.0 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0213080369768073 2 0.019023959670407646 3 4.80858380294664E-4 4 0.08946971238357593 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009226470171903865 12-13 0.0 14-15 0.0 16-17 4.80858380294664E-4 18-19 9.01609463052495E-5 20-21 0.0 22-23 1.3524141945787425E-4 24-25 0.004147403530041477 26-27 0.0032908745401416064 28-29 0.0010067972337419527 30-31 1.80321892610499E-4 32-33 5.860461509841217E-4 34-35 0.002764935686694318 36-37 0.08901890765204967 38-39 0.10203213756877402 40-41 0.08195630019147179 42-43 0.08553268439491335 44-45 0.08454091398555562 46-47 0.08324860708851371 48-49 0.07780889666143032 50-51 0.07747830652497774 52-53 0.060287619429443495 54-55 0.051091202906308045 56-57 0.028220376193543097 58-59 0.02147333204503359 60-61 0.012893015321650679 62-63 0.0020135944674839054 64-65 0.005710193265999136 66-67 0.1539197621674451 68-69 0.7158929404880652 70-71 0.8724123432739783 72-73 0.3557901209448987 74-75 0.053360253388323495 76-77 0.19220811069840774 78-79 0.013253659106871677 80-81 0.0031105526475311077 82-83 0.0017280848041839488 84-85 1.3524141945787425E-4 86-87 0.001848299399257615 88-89 2.8550966329995677E-4 90-91 0.003786759744820479 92-93 0.003771732920436271 94-95 0.0759605972621727 96-97 0.03732663177037329 98-99 0.01305831038987697 100-101 0.011901244912292934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3327383.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.46083921203527 #Duplication Level Percentage of deduplicated Percentage of total 1 61.95961848816509 16.39503502454384 2 13.202012483629384 6.9867265920919905 3 6.354118195739774 5.044058997352135 4 4.061102125888892 4.298406815072023 5 2.725198123491334 3.6055514683322207 6 1.9299865858452354 3.0641438837661408 7 1.4604396544718234 2.7051121213101483 8 1.1335240231562353 2.399519753577319 9 0.896004637900181 2.1338131191043117 >10 5.635710980693634 27.281236832299527 >50 0.3720374366430049 6.8011475306511135 >100 0.24595987802622254 12.376787639453434 >500 0.018012176479107637 3.1953961647438605 >1k 0.0059265870995583395 2.375891144514257 >5k 0.0 0.0 >10k+ 3.486227705622553E-4 1.3371729131875212 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17349 0.5214007524832579 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14875 0.44704802543019545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11262 0.33846419242990666 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4720 0.14185322218692586 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3711 0.11152909057959365 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3502 0.10524787798699459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.40429190147332E-4 2 0.0 0.0 0.0 0.0 2.40429190147332E-4 3 0.0 0.0 0.0 0.0 2.40429190147332E-4 4 0.0 0.0 0.0 0.0 2.40429190147332E-4 5 0.0 0.0 0.0 0.0 2.704828389157485E-4 6 0.0 0.0 0.0 0.0 2.704828389157485E-4 7 0.0 0.0 0.0 0.0 2.704828389157485E-4 8 0.0 0.0 0.0 0.0 2.704828389157485E-4 9 0.0 0.0 0.0 0.0 2.704828389157485E-4 10-11 0.0 0.0 0.0 0.0 2.704828389157485E-4 12-13 0.0 0.0 0.0 0.0 0.001322360545810326 14-15 0.0 0.0 0.0 4.508047315262475E-5 0.0016529506822629074 16-17 0.0 0.0 0.0 1.0518777068945775E-4 0.0019084066967944477 18-19 0.0 0.0 0.0 2.103755413789155E-4 0.0020286212918681134 20-21 0.0 0.0 0.0 3.6064378522099804E-4 0.0022690504820154457 22-23 0.0 0.0 0.0 6.0107297536833E-4 0.0024343455502417364 24-25 0.0 0.0 0.0 0.0011720923019682434 0.002464399199010153 26-27 0.0 0.0 0.0 0.0018783530480260312 0.002599640618468027 28-29 0.0 0.0 0.0 0.004252591300730935 0.002689801564773277 30-31 0.0 0.0 0.0 0.010864394029782565 0.0027348820379259017 32-33 0.0 0.0 0.0 0.02506474307285936 0.0028701234573837754 34-35 0.0 0.0 0.0 0.04601213626444566 0.0030354185256100666 36-37 0.0 0.0 0.0 0.07853018423187232 0.0030354185256100666 38-39 0.0 0.0 0.0 0.12900528733842784 0.0032157404182205657 40-41 0.0 0.0 0.0 0.18639272966171913 0.0032758477157573984 42-43 0.0 0.0 0.0 0.2413458264347687 0.00335098183767844 44-45 0.0 0.0 0.0 0.3098230651536057 0.003426115959599481 46-47 0.0 0.0 0.0 0.3964226540797978 0.003546330554673147 48-49 0.0 0.0 0.0 0.4889878922865207 0.0037116256228994374 50-51 0.0 0.0 0.0 0.5948218164244994 0.0038769206911257285 52-53 0.0 0.0 0.0 0.697409946495489 0.004117349881273061 54-55 0.0 0.0 0.0 0.8054678406423306 0.004237564476346727 56-57 0.0 0.0 0.0 0.9326098017571167 0.004342752247036185 58-59 0.0 1.502682438420825E-5 0.0 1.060052299359587 0.004417886368957225 60-61 0.0 3.00536487684165E-5 0.0 1.1898990888635304 0.004447940017725642 62-63 0.0 3.00536487684165E-5 0.0 1.3179877399145215 0.004598208261567724 64-65 0.0 3.00536487684165E-5 0.0 1.4528534887627904 0.0046583155591045575 66-67 3.00536487684165E-5 3.00536487684165E-5 0.0 1.5970058150805002 0.0046733423834887655 68-69 4.508047315262475E-5 3.00536487684165E-5 0.0 1.7618651053996488 0.0047033960322571825 70-71 6.0107297536833E-5 4.508047315262475E-5 0.0 1.9711436885985174 0.004778530154178224 72-73 6.0107297536833E-5 6.0107297536833E-5 0.0 2.1999871971456244 0.005079066641862388 74-75 6.0107297536833E-5 6.0107297536833E-5 0.0 2.450243930440229 0.005379603129546554 76-77 6.0107297536833E-5 6.0107297536833E-5 0.0 2.7342358844773806 0.005469764075851803 78-79 6.0107297536833E-5 6.0107297536833E-5 0.0 3.0637891700474515 0.0056500859684623025 80-81 6.0107297536833E-5 6.0107297536833E-5 0.0 3.4187227620024503 0.005785327387920177 82-83 6.0107297536833E-5 6.0107297536833E-5 0.0 3.7813050075690118 0.005890515158609634 84-85 6.0107297536833E-5 6.0107297536833E-5 0.0 4.1544511106776705 0.005905541982993843 86-87 6.0107297536833E-5 6.0107297536833E-5 0.0 4.54752278291979 0.006115917524372757 88-89 6.0107297536833E-5 6.0107297536833E-5 0.0 4.970708211227863 0.006281212592599048 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14055 0.0 31.035078 1 GTATCAA 20700 0.0 29.34998 1 GTACATG 13975 0.0 24.895472 1 TCAACGC 25595 0.0 23.432026 4 TACATGG 14590 0.0 23.383234 2 CAACGCA 25760 0.0 23.335958 5 ATCAACG 26005 0.0 23.114202 3 TATCAAC 26105 0.0 23.083998 2 AACGCAG 26360 0.0 22.840761 6 ACATGGG 14530 0.0 22.398432 3 GAGTACT 14790 0.0 22.050594 12-13 AGTACTT 16015 0.0 20.852306 12-13 CCCTATA 990 0.0 20.609346 2 GTACTTT 15840 0.0 20.603868 14-15 ACGCAGA 29335 0.0 20.411245 7 CGCAGAG 29550 0.0 20.150434 8 AGAGTAC 23825 0.0 19.877512 10-11 CATGGGA 7060 0.0 19.422766 4 CATGGGG 6610 0.0 19.38118 4 GCAGAGT 30585 0.0 18.802023 9 >>END_MODULE