FastQCFastQC Report
Fri 27 May 2016
SRR939473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808477
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84760.4686816586553216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68580.3792141122060164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45690.252643522698934No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27710.15322284994500898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27220.15051338778430692No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23400.12939064196005812No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20370.11263621268061469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG97850.026.2708821
TACATGG98200.025.6274032
ACATGGG99800.023.4664963
GAGTACT85950.023.10215612-13
GTATCAA172450.022.9975931
GGTATCA130650.021.5129171
TATAACG2055.300735E-721.4118922
GTACTTT93800.021.18460314-15
AGTACTT90200.020.97801612-13
CATGGGG35750.020.61394
TATCAAC200850.020.0088352
ATCAACG204000.019.7675043
CATGGGA66100.019.6916894
TCAACGC205600.019.504574
CAACGCA205100.019.2555645
AACGCAG207750.019.1988016
ACTTTTT112650.019.18537316-17
AGTACAT73750.018.9134352
TACTTTT103650.018.39449114-15
ATAACGC1851.4289304E-418.1215863
Produced by FastQC (version 0.11.5)