FastQCFastQC Report
Fri 27 May 2016
SRR939473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808477
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118330.6543074642364819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109360.6047077181517929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77160.42665734759137103No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41770.2309678254133174No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39850.2203511573550562No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31850.17611504044563464No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28840.15947120145846477No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.14819099164656227No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26790.1481356965004255No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25390.14039437604127672No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.10008421450756631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT601.7294113E-423.71418234-35
GTACATG114300.022.9312951
TACATGG115450.022.408062
GGTATCA150400.021.7760891
ACATGGG115450.021.4189453
GAGTACT97650.021.20150612-13
TGCACCG3201.8189894E-1120.7252395
CATGGGA78350.020.3756944
GTATCAA191350.020.2120971
GTACTTT104800.019.61941114-15
AGTACTT99800.019.5579912-13
CGCATAG2100.019.52306466-67
TACCTGG13000.018.9524572
GTACCTG13600.018.8193471
AGTACAT89950.018.8049682
ACCGCGC2302.1130127E-618.536868
CGTAGAC1559.80351E-418.337543
GTACCGT1350.00954966417.5451776
ATGGGAT32550.017.3187875
ACTTTTT121650.017.27185816-17
Produced by FastQC (version 0.11.5)