##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939473_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1808477 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96321822174128 31.0 31.0 31.0 30.0 34.0 2 31.2041021257113 31.0 31.0 34.0 30.0 34.0 3 31.193697238062747 31.0 31.0 34.0 28.0 34.0 4 35.12940114803782 35.0 35.0 37.0 33.0 37.0 5 34.759702777530485 35.0 35.0 37.0 32.0 37.0 6 34.73399827589734 35.0 35.0 37.0 32.0 37.0 7 34.59756303231946 35.0 35.0 37.0 30.0 37.0 8 34.61643913635617 35.0 35.0 37.0 30.0 37.0 9 35.9514552853036 37.0 35.0 39.0 30.0 39.0 10-11 35.80257033957302 37.0 35.0 39.0 30.0 39.0 12-13 35.76470256464417 37.0 35.0 39.0 30.0 39.0 14-15 36.582159186984406 38.0 35.0 40.0 30.0 41.0 16-17 36.600898712010164 38.0 35.0 40.0 30.5 41.0 18-19 36.590247208009835 38.0 35.0 40.0 30.0 41.0 20-21 36.51186937959399 38.0 35.0 40.0 30.0 41.0 22-23 36.367081804192146 38.0 34.5 40.0 30.0 41.0 24-25 36.14252932163362 38.0 34.0 40.0 29.5 41.0 26-27 35.74179323264825 38.0 34.0 40.0 27.5 41.0 28-29 35.445852504621286 38.0 34.0 40.0 27.0 41.0 30-31 35.21611693153963 38.0 34.0 40.0 27.0 41.0 32-33 34.975629217291676 37.0 33.0 40.0 27.0 41.0 34-35 34.911536336928805 37.5 33.0 40.0 26.0 41.0 36-37 34.89696247173727 38.0 33.0 40.0 25.5 41.0 38-39 34.835652043128 38.0 33.0 40.0 25.5 41.0 40-41 34.60098276063229 37.5 33.0 40.0 25.0 41.0 42-43 34.28933516986945 37.0 33.0 40.0 24.0 41.0 44-45 34.07095777275575 37.0 33.0 40.0 24.0 41.0 46-47 33.70233019275335 37.0 32.5 40.0 23.0 41.0 48-49 33.35474103347734 37.0 32.0 40.0 21.5 41.0 50-51 33.07444413171967 37.0 31.0 40.0 20.0 41.0 52-53 32.709015652397014 36.0 31.0 39.0 18.5 41.0 54-55 32.484876777531596 36.0 31.0 39.0 18.0 40.0 56-57 32.087617923811024 36.0 30.5 39.0 15.5 40.0 58-59 31.662600353778345 35.0 30.0 39.0 13.5 40.0 60-61 31.35077526559641 35.0 29.0 38.5 12.0 40.0 62-63 30.968229344359923 35.0 29.0 38.0 8.0 40.0 64-65 30.51074578222449 34.0 28.0 38.0 7.5 40.0 66-67 28.794111011641288 33.5 25.0 37.0 4.5 39.5 68-69 25.118598688288543 29.5 15.5 35.0 2.0 38.0 70-71 24.50128975928364 29.0 12.5 35.0 2.0 38.0 72-73 26.32932793726434 31.0 21.0 35.0 2.0 38.0 74-75 26.94903418733 31.0 23.0 35.0 2.0 37.5 76-77 27.011167407713785 31.5 25.0 34.5 2.0 36.5 78-79 27.84160290675524 32.5 26.0 35.0 2.0 37.0 80-81 27.70910135987353 33.0 26.0 35.0 2.0 36.5 82-83 27.301395870669076 32.5 26.0 35.0 2.0 36.0 84-85 26.953508117603928 32.0 25.0 35.0 2.0 36.0 86-87 26.513069560740888 32.0 24.5 34.5 2.0 35.0 88-89 26.01197775808042 32.0 23.5 34.0 2.0 35.0 90-91 25.544058895966053 31.5 22.0 34.0 2.0 35.0 92-93 24.97935804547141 31.0 19.5 34.0 2.0 35.0 94-95 23.6070063373767 31.0 7.0 34.0 2.0 35.0 96-97 22.784945288217656 30.0 2.0 34.0 2.0 35.0 98-99 22.456264304163113 30.0 2.0 34.0 2.0 35.0 100-101 21.220492436453437 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.41697466768019 1101 2 0.30808741660274563 1101 3 0.3437778776039089 1101 4 0.3054181704718175 1101 5 0.40884870362959447 1101 6 0.6420461346292399 1101 7 0.46751444805588704 1101 8 0.4912491551274556 1101 9 0.5510948201435397 1101 10-11 0.7949610307489934 1101 12-13 0.6690468116122261 1101 14-15 0.7531293853734482 1101 16-17 0.841384479197643 1101 18-19 0.9091434162396226 1101 20-21 0.8847083346525295 1101 22-23 1.0330604670584762 1101 24-25 0.8744925341171808 1101 26-27 0.8995919398830168 1101 28-29 0.9294276762130096 1101 30-31 0.9202792034124414 1101 32-33 0.8556472356751073 1101 34-35 0.9897730493786625 1101 36-37 0.9950674300518685 1101 38-39 0.8567452472976171 1101 40-41 1.0208353964354018 1101 42-43 1.11247971091516 1101 44-45 0.7242904546430182 1101 46-47 0.5050889197259281 1101 48-49 0.3944169996728988 1101 50-51 0.1466690436337288 1101 52-53 0.6010633252954065 1101 54-55 0.5020884126383507 1101 56-57 0.3999692191572599 1101 58-59 0.369873242482484 1101 60-61 0.27461133242758606 1101 62-63 0.19606702565349465 1101 64-65 -0.08021780337888629 1101 66-67 -3.0683508619976863 1101 68-69 -7.84058368659457 1101 70-71 -10.943246199925987 1101 72-73 -11.108166671985728 1101 74-75 -9.442409483947383 1101 76-77 -4.555109291262518 1101 78-79 -1.5432062779645186 1101 80-81 -0.9221406790332658 1101 82-83 -0.5618591269976996 1101 84-85 -0.6178292489361681 1101 86-87 -0.7037776422322857 1101 88-89 -0.5542087213136817 1101 90-91 -0.5473783005240165 1101 92-93 -1.280282909746198 1101 94-95 -4.609678402319261 1101 96-97 -1.6483330264167826 1101 98-99 -1.2746880032104606 1101 100-101 -0.9536530312297664 1102 1 0.3808218035861479 1102 2 0.2828838708109629 1102 3 0.28664413958781765 1102 4 0.18676301799312967 1102 5 0.28600460284432216 1102 6 0.4511274084163972 1102 7 0.3714372210963077 1102 8 0.5205103682122001 1102 9 0.6549164224945585 1102 10-11 0.5015762689818857 1102 12-13 0.5718681489923867 1102 14-15 0.5610700800788493 1102 16-17 0.5464056768658949 1102 18-19 0.7262701686604984 1102 20-21 0.851932042317415 1102 22-23 0.887539076684142 1102 24-25 0.7343235549776139 1102 26-27 0.7025048126488329 1102 28-29 0.8574344931904179 1102 30-31 0.6121548357292497 1102 32-33 0.48090377563752895 1102 34-35 0.787882121017148 1102 36-37 0.9207075755449523 1102 38-39 0.7024202003255127 1102 40-41 0.5777728151375285 1102 42-43 0.839599477740812 1102 44-45 0.4713805929096324 1102 46-47 0.6876935832129476 1102 48-49 0.6342704226145486 1102 50-51 0.7154910073086356 1102 52-53 0.8594719344393127 1102 54-55 0.6909740897593224 1102 56-57 0.5954586925879077 1102 58-59 0.6767131037341123 1102 60-61 0.6591400456327712 1102 62-63 0.5487167341855539 1102 64-65 0.6079500436124903 1102 66-67 -2.471459451870981 1102 68-69 -7.757178088483087 1102 70-71 -11.198874421730679 1102 72-73 -9.175952238300706 1102 74-75 -7.587627362253496 1102 76-77 -3.9162007302951345 1102 78-79 -1.0332861305443721 1102 80-81 -0.3587253251767386 1102 82-83 -0.329646499932128 1102 84-85 -0.300326117462447 1102 86-87 -0.3859089714224382 1102 88-89 -0.332994191275624 1102 90-91 -0.5164845056596334 1102 92-93 -0.9019147688780542 1102 94-95 -4.4913303751477045 1102 96-97 -1.1318247400555563 1102 98-99 -0.6338782763713233 1102 100-101 -0.6188942751474791 1103 1 0.32050475278922264 1103 2 0.15252611367301938 1103 3 0.2269749502746663 1103 4 0.11487619308773134 1103 5 0.33426455009807654 1103 6 0.3593890756563951 1103 7 0.43632943529192403 1103 8 0.39181239779595955 1103 9 0.7316536085970711 1103 10-11 0.6594753057895844 1103 12-13 0.5202011956613504 1103 14-15 0.6016072891145114 1103 16-17 0.5806764027000924 1103 18-19 0.4466634721807381 1103 20-21 0.49331634457632845 1103 22-23 0.5254404068068297 1103 24-25 0.7299447566746622 1103 26-27 0.5361921764832616 1103 28-29 0.5692206989007715 1103 30-31 0.6377496643039606 1103 32-33 0.4914558912206033 1103 34-35 0.5516076112132211 1103 36-37 0.4672604364553976 1103 38-39 0.6025549325809934 1103 40-41 0.7428226947384857 1103 42-43 0.7433289812381147 1103 44-45 0.6966672572829822 1103 46-47 0.7976551701869141 1103 48-49 0.5974498171793954 1103 50-51 0.6178918214354567 1103 52-53 0.548734920335427 1103 54-55 0.6082341516788219 1103 56-57 0.6054833457240214 1103 58-59 0.5684801029314492 1103 60-61 0.26403197316401616 1103 62-63 0.30507208334276115 1103 64-65 0.0540084084789072 1103 66-67 -2.978224731082083 1103 68-69 -7.552425193172919 1103 70-71 -11.359784867780444 1103 72-73 -9.19518455110887 1103 74-75 -7.832678101847581 1103 76-77 -4.343720510136894 1103 78-79 -1.3506106919478817 1103 80-81 -0.6255281574207459 1103 82-83 -0.3711540678715828 1103 84-85 -0.5337153503485865 1103 86-87 -0.43285312920250973 1103 88-89 -0.4525303683195432 1103 90-91 -0.3184409900077583 1103 92-93 -0.33444400864413737 1103 94-95 0.4489287694458035 1103 96-97 0.5720380623753591 1103 98-99 0.05350705787933663 1103 100-101 0.028211848003532936 1104 1 0.38434490873464 1104 2 0.21880291679192965 1104 3 0.4321406422824623 1104 4 0.19772219698636917 1104 5 0.41126260078033994 1104 6 0.4110459957343693 1104 7 0.5274600395804256 1104 8 0.3001944640727672 1104 9 0.6117705676613951 1104 10-11 0.6260932395127838 1104 12-13 0.6452011956613504 1104 14-15 0.731967912896188 1104 16-17 0.768298235058765 1104 18-19 0.8214198074633785 1104 20-21 0.8882968513989411 1104 22-23 0.6779745198672629 1104 24-25 0.7126445617428914 1104 26-27 0.8814650809666844 1104 28-29 0.9897860010450188 1104 30-31 1.0960829976372892 1104 32-33 0.9232297703629015 1104 34-35 0.9916660907453902 1104 36-37 1.2048335358705984 1104 38-39 1.0296991820936583 1104 40-41 1.293992285381762 1104 42-43 1.3732022755850934 1104 44-45 1.3387237874974076 1104 46-47 1.1290391857814583 1104 48-49 1.27483773140942 1104 50-51 1.354002932546571 1104 52-53 1.3270000275479035 1104 54-55 1.4152516955384797 1104 56-57 1.280190948063204 1104 58-59 1.2850980366546487 1104 60-61 1.1909325579593393 1104 62-63 1.2290486915298935 1104 64-65 1.138560065398984 1104 66-67 -2.1658465634407555 1104 68-69 -7.066801411496506 1104 70-71 -8.71967765530481 1104 72-73 -8.0967440052999 1104 74-75 -6.565865236350508 1104 76-77 -2.9767614458094087 1104 78-79 0.004896130664207021 1104 80-81 0.636411413729352 1104 82-83 0.8883488561050221 1104 84-85 0.981148197409702 1104 86-87 1.0432677284972947 1104 88-89 1.1193507232983926 1104 90-91 1.0421827916686546 1104 92-93 1.1372908841433862 1104 94-95 2.1307026485881053 1104 96-97 2.2362680818685376 1104 98-99 1.7138384418949322 1104 100-101 1.613981828510358 1105 1 0.4624293324646267 1105 2 0.31022771752886413 1105 3 0.43981791516895896 1105 4 0.37687457985926187 1105 5 0.4681544661893682 1105 6 0.52739039539847 1105 7 0.677330763554572 1105 8 0.6017522141492151 1105 9 1.0073459761518606 1105 10-11 0.9872759471047061 1105 12-13 0.8410363869529434 1105 14-15 0.993904170490147 1105 16-17 0.9946470180842653 1105 18-19 0.9448914296952609 1105 20-21 1.1258227326392003 1105 22-23 1.115602742655632 1105 24-25 1.1154664808220645 1105 26-27 1.1218954125021767 1105 28-29 1.1962769971662652 1105 30-31 1.0684803649696022 1105 32-33 1.2948468662297685 1105 34-35 1.2712125974524966 1105 36-37 1.2382769029359366 1105 38-39 1.1758074814157098 1105 40-41 1.149893776198006 1105 42-43 1.2607831381889625 1105 44-45 1.0766491889347307 1105 46-47 1.1574273679361582 1105 48-49 1.0200336667174383 1105 50-51 1.0721691253747068 1105 52-53 1.3123003824770976 1105 54-55 1.3320877085919918 1105 56-57 1.3044533978036519 1105 58-59 1.3235769439763736 1105 60-61 1.1914181122746719 1105 62-63 1.331834847871047 1105 64-65 1.3086309376310972 1105 66-67 1.8831113468224636 1105 68-69 -4.840677121899272 1105 70-71 -5.071955062437667 1105 72-73 -1.4025116284482166 1105 74-75 1.1548836368037279 1105 76-77 1.3175437582725138 1105 78-79 1.2367539374498016 1105 80-81 1.324126025659858 1105 82-83 1.2485954840882876 1105 84-85 1.0462264399373922 1105 86-87 1.2843088194335834 1105 88-89 1.4179781063369603 1105 90-91 1.3081399312933897 1105 92-93 1.3896932123425998 1105 94-95 2.1344517006743686 1105 96-97 2.1651925394157274 1105 98-99 2.014000119766152 1105 100-101 1.8895998777346534 1106 1 0.09076785301183676 1106 2 0.08539576314186093 1106 3 0.10589521279816694 1106 4 0.01739012090530423 1106 5 0.2642938475362797 1106 6 0.24809341375344474 1106 7 0.2623845467721395 1106 8 0.126076559185627 1106 9 0.45547623221726496 1106 10-11 0.2890472632967658 1106 12-13 0.1810992082800098 1106 14-15 0.195102450423434 1106 16-17 0.2225281968798214 1106 18-19 0.32476767417157504 1106 20-21 0.30675423746766484 1106 22-23 0.30273950596828314 1106 24-25 0.3930934238719104 1106 26-27 0.3056968882956639 1106 28-29 0.46587369727441796 1106 30-31 0.3189987271319623 1106 32-33 0.46652855809488614 1106 34-35 0.571824573737878 1106 36-37 0.4650732212115045 1106 38-39 0.43931543179088095 1106 40-41 0.4531759941606097 1106 42-43 0.6983504929397881 1106 44-45 0.4717315616995137 1106 46-47 0.4625253502392823 1106 48-49 0.5142289444848416 1106 50-51 0.46611898039136435 1106 52-53 0.5705825621223326 1106 54-55 0.444811541899945 1106 56-57 0.5283627417815531 1106 58-59 0.8370159230867067 1106 60-61 0.5448706988407999 1106 62-63 0.5965268049594279 1106 64-65 0.5811690945464747 1106 66-67 1.241500288238079 1106 68-69 -5.120419183737759 1106 70-71 -6.20026089347914 1106 72-73 -2.70851402041 1106 74-75 0.12186184763163865 1106 76-77 0.4022265814579704 1106 78-79 0.18769513178304464 1106 80-81 0.23544598239954695 1106 82-83 0.4209321634859009 1106 84-85 0.5906574653197794 1106 86-87 0.5592731842800802 1106 88-89 0.4003826505853816 1106 90-91 0.4443708719943871 1106 92-93 0.3054570259937357 1106 94-95 1.3095020880150727 1106 96-97 1.4382805505814105 1106 98-99 1.0956509613592722 1106 100-101 0.9832448941296974 1107 1 -0.09351472860981147 1107 2 -0.11906802777324188 1107 3 -0.23375601443149208 1107 4 -0.18594446379112384 1107 5 -0.10983243287630984 1107 6 -0.007275529798164371 1107 7 0.006273723673167808 1107 8 -0.07772877638824127 1107 9 0.0020507747248714736 1107 10-11 0.06361569200184647 1107 12-13 9.178202203941055E-4 1107 14-15 0.07047562477406899 1107 16-17 -0.12049053781332475 1107 18-19 0.0925280917693172 1107 20-21 -0.05468560474141526 1107 22-23 -0.027431156121970446 1107 24-25 0.16379677931266912 1107 26-27 0.06553647546501651 1107 28-29 0.05902874116150514 1107 30-31 0.0681756579554218 1107 32-33 0.03972138122961866 1107 34-35 0.2686267451507902 1107 36-37 0.09889533100967896 1107 38-39 0.05395891248711848 1107 40-41 0.08856305268269438 1107 42-43 -0.002564789778894294 1107 44-45 0.024730512938063498 1107 46-47 -0.23042590022997445 1107 48-49 -0.17010950156055316 1107 50-51 0.062283999429958214 1107 52-53 0.1757335599354306 1107 54-55 0.24020399320106378 1107 56-57 0.23943268701952647 1107 58-59 0.057465132642349204 1107 60-61 0.16439077130067759 1107 62-63 0.3405418630286299 1107 64-65 0.1932204659808363 1107 66-67 1.1172719621447733 1107 68-69 -5.721270855499263 1107 70-71 -6.003206214870893 1107 72-73 -2.4347180104706005 1107 74-75 0.0960576978732739 1107 76-77 0.2617789510033681 1107 78-79 0.3094505260518048 1107 80-81 0.24029650409877945 1107 82-83 0.3299863713432707 1107 84-85 0.3220888757251572 1107 86-87 0.35678103509376413 1107 88-89 0.24029234388224552 1107 90-91 0.1600090373769163 1107 92-93 0.49094776888629355 1107 94-95 1.1258131590031155 1107 96-97 1.043543277241998 1107 98-99 0.7045828633715843 1107 100-101 0.6360765500406025 1108 1 -0.016193291264013254 1108 2 -0.047803031845614186 1108 3 -0.0470238026999894 1108 4 0.03310068070730665 1108 5 0.05632152705116056 1108 6 0.14020731834981603 1108 7 0.09047997373210848 1108 8 0.11045494633916064 1108 9 0.12213706629668053 1108 10-11 0.20427887047590332 1108 12-13 0.15800362092388553 1108 14-15 0.15015313074613346 1108 16-17 -0.015388262000925579 1108 18-19 -0.09407308939186265 1108 20-21 0.13317376769163758 1108 22-23 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1.342572439049981 2316 100-101 1.4979644953877589 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 31.0 4 67.0 5 158.0 6 411.0 7 940.0 8 1895.0 9 3313.0 10 5158.0 11 7612.0 12 9884.0 13 12175.0 14 15333.0 15 18693.0 16 22379.0 17 25634.0 18 23289.0 19 20062.0 20 19254.0 21 19553.0 22 20489.0 23 23053.0 24 28060.0 25 34569.0 26 43797.0 27 54454.0 28 66541.0 29 79266.0 30 92775.0 31 107644.0 32 124447.0 33 143372.0 34 166600.0 35 189389.0 36 196394.0 37 161946.0 38 65891.0 39 3944.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.742853856011344 19.98763324268434 15.227095366171609 22.042417535132707 2 15.886369104076852 21.637543656861904 39.00820458649382 23.467882652567422 3 19.89768186158851 25.588603006839456 30.397345390624263 24.11636974094777 4 13.050041554302322 17.44230089738493 36.53925374776677 32.968403800545985 5 13.049834805568228 38.75270600920665 33.63480280346701 14.56265638175811 6 31.13310260512022 36.58266043748414 16.984235906787866 15.300001050607776 7 27.690150330913806 34.03250359280212 20.166084500936424 18.111261575347655 8 26.26226377222381 35.086650258753636 19.116306151529713 19.53477981749284 9 26.3685410430987 16.93424909468022 20.347286694826643 36.349923167394444 10-11 25.186054342963722 27.05185081148392 26.38665020345849 21.37544464209387 12-13 26.56763121676416 24.583613725803534 27.267999537732578 21.580755519699725 14-15 23.17776228284905 25.70002272630506 26.678608575060675 24.443606415785215 16-17 21.372097170402093 29.030798090653853 27.46978458339842 22.127320155545632 18-19 21.163137454183918 27.35350950411498 30.379382162715267 21.103970878985827 20-21 22.496277254286344 26.072048469513298 30.277216685642117 21.15445759055824 22-23 21.89259249633808 26.19499722694842 30.18025664689128 21.73215362982222 24-25 21.723306406440337 26.363592127519453 30.511059858654548 21.402041607385662 26-27 21.766894769684704 26.24621467967474 30.676349271606636 21.31054127903392 28-29 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27.606337000107356 28.575049334878404 21.730754970288395 54-55 21.555644394891154 27.3444155024948 28.828321365383736 22.271618737230305 56-57 22.01030437399324 27.581791106862795 28.403021170764518 22.004883348379444 58-59 22.279698328329253 27.123821543188747 28.496381189818997 22.100098938663006 60-61 21.9546757875469 27.293100444891767 28.996961242710718 21.755262524850618 62-63 22.09841109306921 27.145289778038528 28.923033026448973 21.83326610244329 64-65 21.852543894040497 27.461901172811825 28.789132205515518 21.896422727632164 66-67 21.35491606048776 27.36876242877317 29.12399257869312 22.15232893204595 68-69 21.365575031778953 27.181652951539885 29.581158983965565 21.871613032715594 70-71 21.16467011987651 27.122280178733604 29.621732622230656 22.091317079159232 72-73 21.753298424441915 27.302381497659095 28.551632202315563 22.392687875583427 74-75 21.435185133909336 27.798689093984073 28.536655196054433 22.22947057605215 76-77 21.81081588528712 27.450065714468213 28.297261118299037 22.441857281945623 78-79 21.657335711971015 27.518141167648615 28.49178075979999 22.332742360580387 80-81 21.71995355138378 27.806823522906356 28.19831900246067 22.27490392324919 82-83 21.734807796836787 27.766319394717215 28.053577678897767 22.445295129548235 84-85 21.645727316410436 27.656917948085596 27.94666451384231 22.75069022166165 86-87 21.728960916485644 27.644640632002627 28.006216292359277 22.620182159152456 88-89 21.806135429329927 27.416809905204058 27.860722652129834 22.91633201333618 90-91 21.54578801368831 27.54392062174143 28.126063610215525 22.784227754354735 92-93 21.685794962693116 27.738559033955383 27.940196184957642 22.635449818393855 94-95 21.118587623583906 27.6047631569978 28.27415779779499 23.0024914216233 96-97 20.88916096231595 27.311076088893373 28.41228066042899 23.38748228836168 98-99 21.382091317804463 27.308598743462852 27.765538939185337 23.543770999547345 100-101 21.359222227199155 27.13614065473198 28.018052991053132 23.48658412701573 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3281.0 1 2210.5 2 1256.0 3 1445.5 4 1318.5 5 1265.0 6 2007.0 7 3624.5 8 5218.5 9 5904.0 10 5385.5 11 4332.5 12 3815.0 13 3667.0 14 3617.5 15 3636.5 16 3698.5 17 3871.0 18 4087.0 19 4378.0 20 4735.5 21 5260.5 22 6017.5 23 7052.0 24 8312.0 25 9883.5 26 11997.5 27 14348.0 28 16567.5 29 18879.5 30 21569.5 31 24506.5 32 27786.5 33 30958.0 34 34060.0 35 37245.5 36 40721.0 37 44013.5 38 46252.5 39 47545.0 40 48855.0 41 50795.5 42 52813.0 43 54909.5 44 57381.5 45 60421.5 46 63713.5 47 69666.0 48 79951.5 49 79101.5 50 68669.0 51 63234.5 52 60374.0 53 58759.0 54 57060.5 55 53491.0 56 49030.0 57 45090.0 58 41021.0 59 36360.0 60 31261.5 61 25881.0 62 20563.5 63 15710.0 64 11452.5 65 8165.0 66 5841.0 67 4264.0 68 3080.0 69 2117.5 70 1477.5 71 1069.5 72 806.5 73 637.5 74 467.0 75 317.0 76 218.0 77 135.0 78 82.5 79 50.5 80 31.0 81 18.0 82 15.5 83 11.0 84 3.5 85 4.0 86 4.5 87 2.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022339239039257896 2 0.018910939978777724 3 0.0 4 0.0 5 1.1059029227355393E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.488281576154963E-4 18-19 0.0012994359342142587 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.805990344361582E-4 28-29 0.019602129305487433 30-31 0.0050595058715150925 32-33 0.009151346685636589 34-35 0.009704298147004358 36-37 0.10265543880292644 38-39 0.10553078640203883 40-41 0.12095813217419962 42-43 0.10594549999806467 44-45 0.10011186208063469 46-47 0.10215778248769546 48-49 0.10459076891771364 50-51 0.10295956210667871 52-53 0.07956971529082205 54-55 0.06743243071379951 56-57 0.038761897441880654 58-59 0.01581441179511821 60-61 0.009510765135525638 62-63 0.0017970922494452513 64-65 0.004865972860036373 66-67 0.42665734759137103 68-69 0.8204140832313599 70-71 0.9081951277234932 72-73 0.28288996763575097 74-75 0.14946278000770816 76-77 0.015233812760682055 78-79 0.008791928235747539 80-81 0.001492968945692978 82-83 0.0 84-85 0.0 86-87 1.935330114787194E-4 88-89 7.46484472846489E-4 90-91 0.00400889809491633 92-93 0.0016865019571716973 94-95 0.22590831954180232 96-97 0.23364964000095106 98-99 0.01401731954567296 100-101 0.007409549582328114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1808477.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.37156924027041 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48712150604125 39.221229937278444 2 14.353893518633972 15.321796435944835 3 5.291629402953268 8.472676952207133 4 2.3959529220830627 5.115030691095376 5 1.3315321916274359 3.5532981280546347 6 0.7785383800345866 2.4931089033734337 7 0.4999534887282592 1.8678311568402521 8 0.34096538137455257 1.455828596845371 9 0.2404762723086593 1.1551136416347358 >10 1.1764510998579394 11.162247175455517 >50 0.06583165252337166 2.3979149292703994 >100 0.03326181646672277 3.19662304137109 >500 0.0020916035080670026 0.7554148365591538 >1k 0.001987023332663653 2.130332353107228 >5k 1.0458017540335012E-4 0.43067687888949546 >10k+ 2.0916035080670024E-4 1.270876342072955 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11833 0.6543074642364819 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10936 0.6047077181517929 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7716 0.42665734759137103 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4177 0.2309678254133174 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3985 0.2203511573550562 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3185 0.17611504044563464 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2884 0.15947120145846477 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2680 0.14819099164656227 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2679 0.1481356965004255 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2539 0.14039437604127672 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1810 0.10008421450756631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.529514613677697E-5 0.0 0.0 0.0 0.0 7 5.529514613677697E-5 0.0 0.0 0.0 0.0 8 5.529514613677697E-5 0.0 0.0 0.0 0.0 9 5.529514613677697E-5 0.0 0.0 0.0 0.0 10-11 5.529514613677697E-5 0.0 0.0 8.294271920516545E-5 0.0 12-13 5.529514613677697E-5 0.0 0.0 1.1059029227355393E-4 0.0 14-15 5.529514613677697E-5 5.529514613677697E-5 0.0 1.658854384103309E-4 0.0 16-17 5.529514613677697E-5 5.529514613677697E-5 0.0 1.935330114787194E-4 0.0 18-19 5.529514613677697E-5 5.529514613677697E-5 0.0 2.2118058454710787E-4 0.0 20-21 5.529514613677697E-5 5.529514613677697E-5 0.0 2.488281576154964E-4 0.0 22-23 5.529514613677697E-5 5.529514613677697E-5 0.0 3.8706602295743883E-4 0.0 24-25 5.529514613677697E-5 5.529514613677697E-5 0.0 6.082466075045467E-4 0.0 26-27 5.529514613677697E-5 5.529514613677697E-5 0.0 0.0012164932150090933 0.0 28-29 5.529514613677697E-5 5.529514613677697E-5 0.0 0.002543576722291741 0.0 30-31 5.529514613677697E-5 5.529514613677697E-5 0.0 0.006137761221182243 0.0 32-33 5.529514613677697E-5 5.529514613677697E-5 0.0 0.015454993345229163 0.0 34-35 5.529514613677697E-5 5.529514613677697E-5 0.0 0.02969349347544923 0.0 36-37 5.529514613677697E-5 5.529514613677697E-5 0.0 0.05200508494163874 0.0 38-39 5.529514613677697E-5 5.529514613677697E-5 0.0 0.08310860464357578 0.0 40-41 5.529514613677697E-5 5.529514613677697E-5 0.0 0.12012870498214798 0.0 42-43 5.529514613677697E-5 5.529514613677697E-5 0.0 0.1551028849136594 0.0 44-45 5.529514613677697E-5 5.529514613677697E-5 0.0 0.1988136979347816 0.0 46-47 5.529514613677697E-5 5.529514613677697E-5 0.0 0.25463414795985795 0.0 48-49 5.529514613677697E-5 5.529514613677697E-5 0.0 0.313993487337688 0.0 50-51 5.529514613677697E-5 5.529514613677697E-5 0.0 0.3828082967049069 0.0 52-53 5.529514613677697E-5 5.529514613677697E-5 0.0 0.452341942971904 0.0 54-55 5.529514613677697E-5 5.529514613677697E-5 0.0 0.5274880465717839 0.0 56-57 5.529514613677697E-5 5.529514613677697E-5 0.0 0.6145226065910708 0.0 58-59 1.1059029227355393E-4 5.529514613677697E-5 0.0 0.6992624180456815 0.0 60-61 1.1059029227355393E-4 5.529514613677697E-5 0.0 0.7866840440879259 0.0 62-63 1.1059029227355393E-4 5.529514613677697E-5 0.0 0.8746586215915382 0.0 64-65 1.3823786534194243E-4 8.294271920516545E-5 0.0 0.9653150136827839 0.0 66-67 1.658854384103309E-4 1.1059029227355393E-4 0.0 1.0612244446570234 0.0 68-69 1.658854384103309E-4 1.1059029227355393E-4 0.0 1.1799984185588204 0.0 70-71 1.658854384103309E-4 1.1059029227355393E-4 0.0 1.336456034552831 0.0 72-73 1.658854384103309E-4 1.1059029227355393E-4 0.0 1.5078986351499077 0.0 74-75 1.658854384103309E-4 1.1059029227355393E-4 0.0 1.6945474009345984 0.0 76-77 1.658854384103309E-4 1.1059029227355393E-4 0.0 1.8935546318808587 0.0 78-79 1.658854384103309E-4 1.1059029227355393E-4 0.0 2.1249095233171333 0.0 80-81 1.658854384103309E-4 1.1059029227355393E-4 0.0 2.3919850791577666 0.0 82-83 1.658854384103309E-4 1.1059029227355393E-4 0.0 2.6632907136778625 0.0 84-85 1.658854384103309E-4 1.3823786534194243E-4 0.0 2.93611696471672 0.0 86-87 1.658854384103309E-4 1.658854384103309E-4 0.0 3.2184539808911037 0.0 88-89 1.658854384103309E-4 1.658854384103309E-4 0.0 3.5189278049983495 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACGT 60 1.7294113E-4 23.714182 34-35 GTACATG 11430 0.0 22.931295 1 TACATGG 11545 0.0 22.40806 2 GGTATCA 15040 0.0 21.776089 1 ACATGGG 11545 0.0 21.418945 3 GAGTACT 9765 0.0 21.201506 12-13 TGCACCG 320 1.8189894E-11 20.725239 5 CATGGGA 7835 0.0 20.375694 4 GTATCAA 19135 0.0 20.212097 1 GTACTTT 10480 0.0 19.619411 14-15 AGTACTT 9980 0.0 19.55799 12-13 CGCATAG 210 0.0 19.523064 66-67 TACCTGG 1300 0.0 18.952457 2 GTACCTG 1360 0.0 18.819347 1 AGTACAT 8995 0.0 18.804968 2 ACCGCGC 230 2.1130127E-6 18.53686 8 CGTAGAC 155 9.80351E-4 18.33754 3 GTACCGT 135 0.009549664 17.545177 6 ATGGGAT 3255 0.0 17.318787 5 ACTTTTT 12165 0.0 17.271858 16-17 >>END_MODULE