FastQCFastQC Report
Fri 27 May 2016
SRR939472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804128
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83280.4616080455488746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62390.3458180350839852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46240.2563011050213732No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27210.15082078433459267No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25590.14184137710849784No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22440.12438141861331348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG100400.026.3369141
TACATGG100950.026.305542
ACATGGG105200.024.5643
GTATCAA184200.023.6887951
GAGTACT81550.023.30767812-13
CATGGGA69800.021.7930764
AGTACTT85100.021.54475212-13
GTACTTT91450.021.43293214-15
GTATAGG4900.020.5185551
TATCAAC210350.020.366452
CATGGGG38100.019.774954
ATCAACG216900.019.7678173
TCAACGC218400.019.6504784
CAACGCA217550.019.3117985
ATGGGAG28900.019.3047355
ACTTTTT111200.019.27848216-17
AACGCAG221800.019.0135426
GGTATCA142550.018.8668521
TACTTTT103150.018.05866414-15
TAACCCG1350.00832553517.9680165
Produced by FastQC (version 0.11.5)