FastQCFastQC Report
Fri 27 May 2016
SRR939472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804128
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114650.635487060785044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102650.5689729331843416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76420.42358413593713967No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40820.22625889072172262No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40380.22382003937636355No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31690.17565272530552156No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28000.1551996310683056No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25560.14167509178949608No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25330.1404002376771493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24250.1344139661930861No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18240.10110147395306762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG105400.024.4094831
TACATGG107500.023.5326272
ACATGGG107950.022.2870523
GTACCGT1503.083166E-522.1209816
GAGTACT96150.021.32113612-13
GTCTAGA6300.021.096841
GGTATCA156950.019.9307651
AGTACTT99100.019.89725712-13
CATGGGA72200.019.850584
ACCGTCC2151.1010616E-619.8427418
GTACTTT103100.019.79192414-15
TACCTGG11600.019.6397042
GTATCAA196150.019.1420331
ATAGCCC6200.019.1344343
CGCATAG2350.018.39541866-67
ACTTTTT121100.017.65224516-17
TAGGCCC2704.9233495E-717.5767964
TACTTTT112800.017.39661414-15
GTACCTG14300.017.261051
AGTACAT80350.017.2483922
Produced by FastQC (version 0.11.5)