FastQCFastQC Report
Fri 27 May 2016
SRR939471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413173
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165900.6874766127418134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130830.5421492781495566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88260.3657425306847043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60690.2514946089650431No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43170.17889310049465995No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31930.1323154204029301No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30450.1261824162627379No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25730.10662310576158443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.10119456831317108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG5150.035.4121559
GTATCAA242850.029.2141131
GGTATCA168300.026.3467271
GTACATG156000.024.8064581
GAGTACT140900.024.3712212-13
TATCAAC290650.024.0930752
TACATGG161700.023.7125472
ATCAACG295200.023.5257933
TCAACGC297350.023.3051644
CAACGCA296100.023.1501525
AACGCAG300500.022.5760946
CATGGGT18550.022.4919764
GTACTTT155500.022.40774314-15
AGTACTT150200.022.28746612-13
ACATGGG164150.022.2503033
CCTTTAG9850.021.9968431
AACGCCG3600.021.6451036
TTAGGCA9950.021.2071274
CGTCACT3351.4551915E-1121.0577221
CATGGGA117950.020.8172954
Produced by FastQC (version 0.11.5)