##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939471_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2413173 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.250703119917222 31.0 27.0 31.0 10.0 33.0 2 27.381771219883532 31.0 27.0 33.0 16.0 34.0 3 27.91707142422031 31.0 27.0 33.0 16.0 34.0 4 31.703113701338445 35.0 32.0 37.0 19.0 37.0 5 32.064738417013615 35.0 32.0 37.0 19.0 37.0 6 32.15232186005728 35.0 32.0 37.0 19.0 37.0 7 31.58476536908046 35.0 32.0 37.0 17.0 37.0 8 31.539191346828428 35.0 32.0 37.0 17.0 37.0 9 32.57037228578307 37.0 31.0 39.0 17.0 39.0 10-11 32.27506047017765 36.0 30.0 39.0 17.0 39.0 12-13 32.12537331554762 35.0 30.0 39.0 17.0 39.0 14-15 32.86901249931107 37.0 31.0 39.0 17.0 41.0 16-17 32.92575314741214 37.0 31.0 39.0 17.0 41.0 18-19 33.17382570582383 37.0 31.5 39.5 17.5 41.0 20-21 33.05264458868055 37.0 31.0 40.0 18.0 41.0 22-23 32.858445291738306 37.0 31.0 40.0 17.0 41.0 24-25 32.703728037732894 37.0 30.5 40.0 16.5 41.0 26-27 32.696276437702565 37.0 30.0 40.0 16.0 41.0 28-29 32.74989609116297 37.0 30.5 39.5 16.5 41.0 30-31 32.402286118732476 36.5 30.0 39.0 16.0 41.0 32-33 31.78935637851078 36.0 30.0 39.0 12.5 40.5 34-35 31.352022005881885 35.0 29.5 39.0 9.5 40.0 36-37 31.101009127816365 35.0 28.5 39.0 9.0 40.0 38-39 30.69467439756702 35.0 27.0 39.0 9.0 40.0 40-41 30.61541070615327 35.0 27.0 39.0 9.0 40.0 42-43 30.112655619800154 35.0 26.0 38.0 8.0 40.0 44-45 29.871280260470343 35.0 25.5 38.0 4.5 40.0 46-47 29.19170279130423 34.5 24.0 38.0 2.0 40.0 48-49 28.5984943889228 34.0 23.5 38.0 2.0 40.0 50-51 28.122381611264505 33.5 23.0 37.5 2.0 39.5 52-53 28.523996207482845 34.0 24.0 38.0 2.0 39.5 54-55 28.445525455489516 34.0 23.5 38.0 2.0 40.0 56-57 28.033719505398082 34.0 23.0 38.0 2.0 40.0 58-59 27.610347455404153 33.0 22.0 38.0 2.0 40.0 60-61 27.09781084903569 33.0 21.0 38.0 2.0 40.0 62-63 26.588948450857025 32.5 19.0 37.0 2.0 39.0 64-65 26.082521642667146 32.0 18.0 37.0 2.0 39.0 66-67 25.662148963211507 31.5 13.5 36.5 2.0 39.0 68-69 25.23627149814787 31.0 13.0 36.0 2.0 39.0 70-71 24.769971941506057 31.0 8.0 36.0 2.0 38.5 72-73 24.11980160560391 30.5 7.5 35.0 2.0 38.0 74-75 23.463568919426827 30.0 4.0 35.0 2.0 37.5 76-77 22.785300929523082 29.0 2.0 34.0 2.0 36.5 78-79 22.019798207588103 29.0 2.0 34.0 2.0 36.0 80-81 21.28346662257534 28.0 2.0 34.0 2.0 35.5 82-83 20.42865451420184 26.5 2.0 33.0 2.0 35.0 84-85 19.584437170480527 26.0 2.0 32.5 2.0 35.0 86-87 18.979519910093472 25.5 2.0 32.0 2.0 34.5 88-89 18.097277526310798 24.0 2.0 32.0 2.0 34.0 90-91 17.22373903570113 20.5 2.0 31.0 2.0 34.0 92-93 16.33686084669437 17.5 2.0 31.0 2.0 34.0 94-95 15.418452800524454 4.5 2.0 31.0 2.0 34.0 96-97 14.342769871865798 2.0 2.0 30.0 2.0 34.0 98-99 13.276939531479922 2.0 2.0 29.5 2.0 34.0 100-101 11.85609444494862 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.734123383539462 1101 2 3.6264857349695276 1101 3 2.957939361214976 1101 4 2.952267822972143 1101 5 2.5017723074935994 1101 6 2.378387993203475 1101 7 3.00617683061326 1101 8 2.771404804717708 1101 9 2.9710340020937736 1101 10-11 3.1491659543799884 1101 12-13 3.1149474280821323 1101 14-15 2.9585725981074233 1101 16-17 2.514339040515061 1101 18-19 2.7970490725643273 1101 20-21 2.582965174526933 1101 22-23 2.935416100271766 1101 24-25 3.1504435439471834 1101 26-27 3.1822120669526868 1101 28-29 2.8199417871062167 1101 30-31 2.792373277952734 1101 32-33 2.9720635934159105 1101 34-35 3.0910617415204342 1101 36-37 3.130854912887642 1101 38-39 3.26168478160643 1101 40-41 3.055063016644038 1101 42-43 2.8278164311762843 1101 44-45 2.566099142240926 1101 46-47 2.5164837188894467 1101 48-49 2.343249233839174 1101 50-51 2.6258352151075215 1101 52-53 2.3242612610510207 1101 54-55 2.264626446985936 1101 56-57 2.478615251284527 1101 58-59 2.6165227946416003 1101 60-61 2.6372018829690376 1101 62-63 2.5377969333190826 1101 64-65 2.5274220728714205 1101 66-67 2.4987700384067466 1101 68-69 2.3730096692621743 1101 70-71 2.1995590708461172 1101 72-73 2.1014640834709226 1101 74-75 1.9457533973309218 1101 76-77 1.8934731682108144 1101 78-79 1.8775820268767305 1101 80-81 1.9904165890816472 1101 82-83 1.9732636405337693 1101 84-85 2.145341874051411 1101 86-87 1.4866929110496905 1101 88-89 1.2095858490507752 1101 90-91 1.3103827213048937 1101 92-93 1.314735308737383 1101 94-95 1.1840429868278903 1101 96-97 1.1346344901287413 1101 98-99 0.8787934590420541 1101 100-101 0.7660331188632696 1102 1 3.672214818829513 1102 2 3.6248916363921566 1102 3 3.0510343939649083 1102 4 2.855028620173794 1102 5 2.3823534962227164 1102 6 2.167172444007747 1102 7 2.6691782321557476 1102 8 2.7725922339172833 1102 9 2.935634316115795 1102 10-11 3.123593112179215 1102 12-13 3.230564877488746 1102 14-15 2.961255983872306 1102 16-17 2.5497753575330435 1102 18-19 2.5728842886412693 1102 20-21 2.7427743268084868 1102 22-23 2.8904444479962947 1102 24-25 2.9899664424803376 1102 26-27 2.844349478412134 1102 28-29 2.7340256277767523 1102 30-31 2.857348962884302 1102 32-33 2.974092077777456 1102 34-35 3.3113096785526466 1102 36-37 3.146255996031833 1102 38-39 2.9891974967104566 1102 40-41 2.759618280970219 1102 42-43 2.8112403993195976 1102 44-45 2.611243981535207 1102 46-47 2.591108198051977 1102 48-49 2.623200903558157 1102 50-51 2.3549169057305086 1102 52-53 2.3290365061183493 1102 54-55 2.326942167326422 1102 56-57 2.547101194771262 1102 58-59 2.3894975479129066 1102 60-61 2.682729646298803 1102 62-63 2.402418901796615 1102 64-65 2.2919121209796494 1102 66-67 2.1236347993624705 1102 68-69 2.1354623183752217 1102 70-71 1.9967639604092042 1102 72-73 1.79070773515771 1102 74-75 1.716457431332305 1102 76-77 1.5439963518501578 1102 78-79 1.6897718482777186 1102 80-81 1.4978426409800534 1102 82-83 1.7099644253943218 1102 84-85 1.6537312736767724 1102 86-87 1.1719411860275635 1102 88-89 0.9569242230961592 1102 90-91 0.8054284541858401 1102 92-93 0.9563840556397487 1102 94-95 1.0199223125120405 1102 96-97 0.8422278615961734 1102 98-99 0.49898670884905094 1102 100-101 0.3509356843489524 1103 1 3.6475983717878897 1103 2 3.6524174848808535 1103 3 2.990414589407848 1103 4 2.8855092251478744 1103 5 2.489694892604934 1103 6 2.392046917726212 1103 7 2.899312803039617 1103 8 2.811317359707612 1103 9 3.01947900679599 1103 10-11 3.0664273578426062 1103 12-13 3.2423675942555406 1103 14-15 3.0479637890084277 1103 16-17 2.7399535375968966 1103 18-19 2.74690916308392 1103 20-21 2.897597633209415 1103 22-23 2.977873429306591 1103 24-25 3.1928156087087274 1103 26-27 3.1598729858352996 1103 28-29 2.8783415541984567 1103 30-31 3.0513020955971797 1103 32-33 3.2002364149058025 1103 34-35 3.237499168796191 1103 36-37 3.2701582720781275 1103 38-39 3.26874047946281 1103 40-41 3.031594738598823 1103 42-43 2.976794987370038 1103 44-45 2.5945381055414707 1103 46-47 2.722504073912134 1103 48-49 2.7640689151156153 1103 50-51 2.6647008262510887 1103 52-53 2.273929823923279 1103 54-55 2.3221826841626196 1103 56-57 2.638217292175245 1103 58-59 2.4442945887762413 1103 60-61 2.6706315004811145 1103 62-63 2.527302058209056 1103 64-65 2.250475789046625 1103 66-67 2.415356697178808 1103 68-69 2.3551283406482746 1103 70-71 2.1328405694180184 1103 72-73 2.1290901042253267 1103 74-75 2.411552439264778 1103 76-77 2.25643034240699 1103 78-79 2.375270382471225 1103 80-81 1.931380113405897 1103 82-83 1.7668794067537128 1103 84-85 1.7868753377429378 1103 86-87 1.4650390345522943 1103 88-89 1.5292405794587722 1103 90-91 1.246376194308926 1103 92-93 0.8847348998492315 1103 94-95 0.6554901529318897 1103 96-97 0.6429559186888483 1103 98-99 0.55465815446634 1103 100-101 0.23497853926843604 1104 1 3.6699509871992326 1104 2 3.4742028466852553 1104 3 2.89212612318245 1104 4 2.9000492085941794 1104 5 2.430156791357131 1104 6 2.158244985335749 1104 7 2.8264849928327926 1104 8 2.701531797617985 1104 9 3.0931142922265664 1104 10-11 3.2494346646337178 1104 12-13 3.4379777885322085 1104 14-15 3.241498889597459 1104 16-17 2.952912685443586 1104 18-19 2.9227945806303524 1104 20-21 3.2838757425168836 1104 22-23 3.0633825519366553 1104 24-25 3.098259867882639 1104 26-27 2.882679138693952 1104 28-29 2.9322060636565013 1104 30-31 2.9400358103572373 1104 32-33 3.245950461898026 1104 34-35 3.392244088670026 1104 36-37 3.4747416365803474 1104 38-39 3.393775921501952 1104 40-41 3.188121412729938 1104 42-43 3.2651333197904187 1104 44-45 3.1811034009380528 1104 46-47 3.2246230554014375 1104 48-49 3.38436643519843 1104 50-51 3.184916060476212 1104 52-53 2.879293275247935 1104 54-55 3.032573566117403 1104 56-57 3.3733823103095055 1104 58-59 3.3266559757152336 1104 60-61 3.2956085762685596 1104 62-63 3.0904638440293475 1104 64-65 2.8819165878709754 1104 66-67 2.813965167313384 1104 68-69 2.6856003200213117 1104 70-71 2.72005739506611 1104 72-73 2.773399379603031 1104 74-75 2.761152411755724 1104 76-77 2.4437466470721922 1104 78-79 2.641208441248903 1104 80-81 2.6982648516717163 1104 82-83 2.7425963313378787 1104 84-85 2.6512909874995785 1104 86-87 2.2834111017612635 1104 88-89 2.365034746573869 1104 90-91 2.3088054610478217 1104 92-93 2.1044960536851782 1104 94-95 2.110921110029828 1104 96-97 1.8327302418816362 1104 98-99 1.4435814464315246 1104 100-101 1.073038282179425 1105 1 3.7220865349932772 1105 2 3.387983640645775 1105 3 1.9483365646606323 1105 4 2.6958845184844513 1105 5 2.4528330773339526 1105 6 2.5428337260708247 1105 7 2.966239417830966 1105 8 2.8099633713078127 1105 9 3.2073909978508226 1105 10-11 3.333693793339542 1105 12-13 3.4756198467164694 1105 14-15 3.280067813581759 1105 16-17 3.255127021684899 1105 18-19 2.7488538857028075 1105 20-21 3.162694032310206 1105 22-23 3.3531229966530063 1105 24-25 2.9930364402760006 1105 26-27 3.3231149231968615 1105 28-29 3.097661578877684 1105 30-31 3.2635903149354846 1105 32-33 3.5670215994269583 1105 34-35 3.5278851592728415 1105 36-37 3.6786253021146536 1105 38-39 3.499534886507032 1105 40-41 3.3220043434032753 1105 42-43 3.50611992142289 1105 44-45 3.314886707167158 1105 46-47 3.40398454486305 1105 48-49 3.52552351860097 1105 50-51 3.1867623239770424 1105 52-53 2.946835586511142 1105 54-55 3.15477093782442 1105 56-57 3.3369535195321873 1105 58-59 3.351088310553738 1105 60-61 3.4936839275978215 1105 62-63 3.4117793377663013 1105 64-65 3.329683207976533 1105 66-67 3.514344800671534 1105 68-69 3.3658168321680257 1105 70-71 3.152046087168923 1105 72-73 3.11711230049017 1105 74-75 3.07498479534598 1105 76-77 2.9395671253173177 1105 78-79 3.0243247715324664 1105 80-81 3.0036656765083443 1105 82-83 2.8748881834623425 1105 84-85 3.1038325609145794 1105 86-87 2.809379176533067 1105 88-89 2.9673126064675444 1105 90-91 2.864818894067337 1105 92-93 2.742395402825391 1105 94-95 2.5027786264626357 1105 96-97 2.4729319313449256 1105 98-99 2.1750309118948508 1105 100-101 2.0369996249078266 1106 1 3.152722709055876 1106 2 2.266645959611836 1106 3 2.5017674962415803 1106 4 1.421854056208634 1106 5 1.6283477647647082 1106 6 1.7741924950180277 1106 7 2.3784008482283774 1106 8 2.3376820171614803 1106 9 2.9076815620001923 1106 10-11 2.465540504608292 1106 12-13 2.8163268567966995 1106 14-15 2.483453032671534 1106 16-17 1.1423052915955125 1106 18-19 2.2403233331760006 1106 20-21 1.6514660025552672 1106 22-23 2.2530351670351294 1106 24-25 2.537740615154526 1106 26-27 2.5102065374861624 1106 28-29 1.8812544632845203 1106 30-31 1.3255147447767115 1106 32-33 1.6120658664348682 1106 34-35 2.2509359214167404 1106 36-37 2.4180919114511497 1106 38-39 1.0549648407358454 1106 40-41 1.0060720360451008 1106 42-43 2.3270957913910273 1106 44-45 2.4241287749860057 1106 46-47 2.6585925547481004 1106 48-49 2.7836574804137975 1106 50-51 2.525983347907495 1106 52-53 2.2398906630924103 1106 54-55 2.3598802442419213 1106 56-57 2.856798830810039 1106 58-59 2.767665875772913 1106 60-61 2.8471462635868363 1106 62-63 2.49661856627446 1106 64-65 2.5455783143186075 1106 66-67 2.853152944685693 1106 68-69 2.8328455789413702 1106 70-71 2.562425904407011 1106 72-73 2.6102960764446657 1106 74-75 2.4747008349272726 1106 76-77 2.482087739963216 1106 78-79 2.5556627020406744 1106 80-81 2.405489333419588 1106 82-83 2.3022018005017273 1106 84-85 2.316588106947716 1106 86-87 2.1148087638879467 1106 88-89 2.202546371195698 1106 90-91 2.2086977493619884 1106 92-93 2.1621371089065455 1106 94-95 1.8831063329282713 1106 96-97 2.2418599844330505 1106 98-99 2.1799766659161577 1106 100-101 1.8865199005622184 1107 1 2.7228017018505604 1107 2 3.032442703790455 1107 3 -0.5591124146755675 1107 4 1.769482747971228 1107 5 0.4094509054578275 1107 6 1.4103821125686267 1107 7 2.320764708698153 1107 8 1.5681721325365494 1107 9 -1.8818934506668192 1107 10-11 -0.7396539140665865 1107 12-13 1.6648402794742054 1107 14-15 1.2885246285118157 1107 16-17 -0.736592191988187 1107 18-19 0.5415797265691182 1107 20-21 0.6685992798488627 1107 22-23 0.7239734115396743 1107 24-25 0.17608747206592312 1107 26-27 0.8242009364164815 1107 28-29 1.011998427035806 1107 30-31 1.3933189744699348 1107 32-33 -0.1992665725856284 1107 34-35 1.0234888651722969 1107 36-37 2.082535789691036 1107 38-39 -0.02370884686732211 1107 40-41 0.9036438895810512 1107 42-43 0.5661953374111199 1107 44-45 0.8911693544310388 1107 46-47 1.044259594529052 1107 48-49 0.5992156201850207 1107 50-51 1.4130253936771489 1107 52-53 1.6387475106106173 1107 54-55 1.110300542239738 1107 56-57 1.1154842635282414 1107 58-59 1.0738957228831651 1107 60-61 1.6543585481931835 1107 62-63 1.7991855780355444 1107 64-65 1.9519114574652825 1107 66-67 1.0560110436611545 1107 68-69 0.5405850145080144 1107 70-71 0.15759596176788548 1107 72-73 0.7916509603945059 1107 74-75 0.5029235188239802 1107 76-77 0.6088303796669337 1107 78-79 1.300724309451855 1107 80-81 1.5016125743744695 1107 82-83 1.4870945918466383 1107 84-85 1.7107320274351778 1107 86-87 1.6568041558539441 1107 88-89 1.9778677648719203 1107 90-91 1.9409757645101386 1107 92-93 1.8372131472748912 1107 94-95 1.7302468547365848 1107 96-97 1.6386916343966806 1107 98-99 1.7367955835362974 1107 100-101 1.5843476350035726 1108 1 2.953324725076424 1108 2 3.151997425077411 1108 3 2.628864053689341 1108 4 2.657754083682999 1108 5 2.3762775784372643 1108 6 2.0698800161042428 1108 7 1.9490727459383272 1108 8 2.343577048002519 1108 9 2.74358828012317 1108 10-11 -0.5863443365172714 1108 12-13 1.9874060299464134 1108 14-15 1.7506228740746934 1108 16-17 1.7878460454750211 1108 18-19 0.38665564772759353 1108 20-21 0.03643403691387448 1108 22-23 0.8158520297472833 1108 24-25 -0.31071747504964975 1108 26-27 0.790322783946884 1108 28-29 0.1758272796062812 1108 30-31 0.032493731869450926 1108 32-33 1.4191543239271809 1108 34-35 -0.8870090956871266 1108 36-37 0.6926292246290231 1108 38-39 1.158650961056484 1108 40-41 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-0.6561761836307376 2312 70-71 -1.3023715177355975 2312 72-73 -0.99514077790095 2312 74-75 -0.8501035622741533 2312 76-77 -1.417785484942602 2312 78-79 0.3911671114824564 2312 80-81 0.6198979786067014 2312 82-83 -0.907133898004755 2312 84-85 -1.6165446853490195 2312 86-87 -0.26671063318719135 2312 88-89 -2.0400833814131474 2312 90-91 -1.91095617891731 2312 92-93 -1.7359560188147487 2312 94-95 -1.4447669930090363 2312 96-97 -1.1756014633093823 2312 98-99 -0.505637420982584 2312 100-101 -0.4786915564090517 2313 1 -2.2631791243364496 2313 2 -0.7295822077189946 2313 3 -2.6374588023003085 2313 4 -2.054222400476192 2313 5 -1.9937667451825014 2313 6 -3.5609087659387484 2313 7 0.1291607335890248 2313 8 -3.0035645526021995 2313 9 -4.1845414595003945 2313 10-11 -5.37056575374131 2313 12-13 -3.9729985903234244 2313 14-15 -3.803882211317699 2313 16-17 -2.2498889939447793 2313 18-19 -5.173431676586613 2313 20-21 -7.058442194615292 2313 22-23 -5.823894043544275 2313 24-25 -1.8491542645160166 2313 26-27 -5.70891981319912 2313 28-29 -6.308528285243483 2313 30-31 -5.489659364693978 2313 32-33 -4.8929138280885525 2313 34-35 0.08512524381898601 2313 36-37 0.5293769607590164 2313 38-39 -2.855550998297101 2313 40-41 -6.67330677242116 2313 42-43 -3.4446995781229717 2313 44-45 -1.6667210101603551 2313 46-47 -3.4677988556737276 2313 48-49 -3.56915636004263 2313 50-51 -2.9165232271085877 2313 52-53 -4.463173998527388 2313 54-55 -3.388450786408498 2313 56-57 -3.215650995442072 2313 58-59 -1.2201586407588287 2313 60-61 0.5950182800900308 2313 62-63 0.3094922764865693 2313 64-65 0.8359991534086468 2313 66-67 0.26494420362524096 2313 68-69 -3.1219512330797805 2313 70-71 -1.2273302015087921 2313 72-73 -2.605212397809055 2313 74-75 -2.4702481064094997 2313 76-77 -1.535455658949001 2313 78-79 -1.343489491978442 2313 80-81 -1.311388571100899 2313 82-83 -0.9858577419304346 2313 84-85 -1.4670514261214755 2313 86-87 -1.384435537447608 2313 88-89 -1.9127883551656417 2313 90-91 -3.7477619422688058 2313 92-93 -4.255518759890009 2313 94-95 -3.2028537716630083 2313 96-97 -2.846868668173931 2313 98-99 -2.157036392769905 2313 100-101 -1.8457783672873784 2314 1 -2.442628034324283 2314 2 -0.7842446545970105 2314 3 -2.5564001987866156 2314 4 -1.509730303588885 2314 5 -2.5623203287061465 2314 6 -2.7305679460769703 2314 7 -1.2682651353937295 2314 8 0.41126944307011826 2314 9 -1.9573382083450461 2314 10-11 -2.6894684888117126 2314 12-13 -1.7745066852446953 2314 14-15 -4.428811032354307 2314 16-17 -2.1635444158337336 2314 18-19 -1.269813237788533 2314 20-21 -1.6159353164796713 2314 22-23 -2.3549340656022615 2314 24-25 -2.739802397679121 2314 26-27 -2.507012067129697 2314 28-29 -2.2368300900422895 2314 30-31 -2.0841827803251753 2314 32-33 -8.883632091261909 2314 34-35 -9.520729113532376 2314 36-37 -5.843713944517518 2314 38-39 -4.049441745331563 2314 40-41 -3.623547432242656 2314 42-43 -2.3861245360149965 2314 44-45 -2.1207483908344464 2314 46-47 -4.0031613139403746 2314 48-49 -3.206490550520531 2314 50-51 -2.470631976576616 2314 52-53 -1.9303185382767154 2314 54-55 -0.9625623230948861 2314 56-57 -1.3232630985631566 2314 58-59 -4.0890072798170465 2314 60-61 -4.886468446157178 2314 62-63 -4.3074187962628265 2314 64-65 -5.005966315053826 2314 66-67 -4.652352584180527 2314 68-69 -5.511168294452528 2314 70-71 -7.53906484033924 2314 72-73 -7.436281490854196 2314 74-75 -7.525348116599332 2314 76-77 -8.689600822076201 2314 78-79 -7.607441318056015 2314 80-81 -6.634159695518733 2314 82-83 -5.5496797975189605 2314 84-85 -5.3030093900734485 2314 86-87 -5.316909928005831 2314 88-89 -5.341739012409713 2314 90-91 -5.83970942867737 2314 92-93 -6.059596040763697 2314 94-95 -5.572151212816738 2314 96-97 -5.738469850194274 2314 98-99 -5.942334415343378 2314 100-101 -5.532238779795696 2315 1 -25.03244839360572 2315 2 -13.187096440012962 2315 3 -8.343437357948467 2315 4 -5.705058809572513 2315 5 -2.131853069392175 2315 6 -2.258095490107852 2315 7 -7.755536188002168 2315 8 -1.3564989848317879 2315 9 -4.73514602882743 2315 10-11 -3.130775302497117 2315 12-13 -3.6787213836012853 2315 14-15 -3.466874223153326 2315 16-17 -5.552136175046584 2315 18-19 -6.789799696588993 2315 20-21 -5.506280726980258 2315 22-23 -7.179422203863183 2315 24-25 -24.497804015588674 2315 26-27 -10.734012361694752 2315 28-29 -7.199987475711481 2315 30-31 -5.30010940724754 2315 32-33 -6.164968647224871 2315 34-35 -6.991136866308764 2315 36-37 -5.443851774584257 2315 38-39 -4.138830698702353 2315 40-41 -4.3409485331245925 2315 42-43 -3.912757393647748 2315 44-45 -9.022035024615342 2315 46-47 -5.499399564311879 2315 48-49 -4.8206592395741446 2315 50-51 -6.453327928285535 2315 52-53 -9.911348219003369 2315 54-55 -5.840584362716083 2315 56-57 -12.440949531863954 2315 58-59 -7.541980738186652 2315 60-61 -4.931992486182853 2315 62-63 -5.241100441782361 2315 64-65 -5.598528987066626 2315 66-67 -8.580703481504823 2315 68-69 -5.692402741857279 2315 70-71 -5.851655518014727 2315 72-73 -6.154756435238557 2315 74-75 -5.4610230222945475 2315 76-77 -6.408614886458448 2315 78-79 -5.993395730816399 2315 80-81 -5.358684214762183 2315 82-83 -4.737813442124022 2315 84-85 -4.922973206933122 2315 86-87 -4.825467152763363 2315 88-89 -5.902113307871652 2315 90-91 -5.633607865284333 2315 92-93 -5.523002377869222 2315 94-95 -6.005155798359308 2315 96-97 -5.941039380736713 2315 98-99 -5.684644948462163 2315 100-101 -5.176970241981456 2316 1 -1.3163990108896684 2316 2 0.293681797026494 2316 3 -4.0062720600024555 2316 4 0.3150542836153072 2316 5 -3.978455137278303 2316 6 -0.385511146096686 2316 7 -3.423836708297305 2316 8 -3.217185581038045 2316 9 -1.8356926106515452 2316 10-11 -0.876191750108049 2316 12-13 -3.4085267110367283 2316 14-15 -2.824347120671497 2316 16-17 -1.038028985788472 2316 18-19 -5.30520233613505 2316 20-21 -1.5889912622013966 2316 22-23 -4.292084456179133 2316 24-25 -0.9470299179472228 2316 26-27 -1.436684615037997 2316 28-29 -3.9748427478542396 2316 30-31 -7.38969829251068 2316 32-33 -4.617916608646894 2316 34-35 -1.630655351591237 2316 36-37 -3.894370861136956 2316 38-39 -3.716912359658462 2316 40-41 -1.7488174683022244 2316 42-43 -2.2849565017132285 2316 44-45 0.7694095648591066 2316 46-47 -1.2058146492450774 2316 48-49 -0.8935084899503174 2316 50-51 1.1250090459051663 2316 52-53 2.022552468680562 2316 54-55 2.2794207888704854 2316 56-57 0.2767488117725527 2316 58-59 -2.2839168434059154 2316 60-61 -5.69479931528312 2316 62-63 -1.2785865430737324 2316 64-65 -2.783073623071534 2316 66-67 -1.7068331292632024 2316 68-69 1.250949445376456 2316 70-71 2.3415720340601105 2316 72-73 2.769356615648846 2316 74-75 2.650011041627426 2316 76-77 1.682048882629612 2316 78-79 1.3731438079881926 2316 80-81 2.269887149063706 2316 82-83 1.9907855680462063 2316 84-85 2.043839094028307 2316 86-87 1.2106132150085536 2316 88-89 -0.8990431284574534 2316 90-91 -1.110485777214862 2316 92-93 -0.9150671972162243 2316 94-95 -2.353754672563909 2316 96-97 -4.156409690677917 2316 98-99 -2.3385512409514213 2316 100-101 -1.4870678048731119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28216.0 3 8485.0 4 8820.0 5 10193.0 6 11622.0 7 14806.0 8 18981.0 9 23091.0 10 26988.0 11 31039.0 12 35901.0 13 40172.0 14 44056.0 15 48724.0 16 52319.0 17 53564.0 18 49185.0 19 48290.0 20 51307.0 21 55591.0 22 60790.0 23 66319.0 24 71848.0 25 78479.0 26 85251.0 27 92251.0 28 99554.0 29 107440.0 30 115902.0 31 125740.0 32 135207.0 33 145231.0 34 153628.0 35 155952.0 36 144698.0 37 97055.0 38 16393.0 39 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33571424880596 21.85354011185193 15.752236091279718 20.058509548062396 2 14.800730129394005 22.35725888416807 41.83035142543836 21.01165956099957 3 17.642142677126127 29.34865154889569 31.688317896724072 21.320887877254112 4 11.239881765676119 19.36771687075166 40.50078324586634 28.89161811770588 5 11.1504773935435 39.97054718899892 35.35539390202836 13.52358151542922 6 26.26884224563098 40.21481124665952 19.42097137634122 14.095375131368277 7 25.3468078711659 36.07786791520459 22.665469531168426 15.909854682461077 8 24.955337685614985 35.24075363442593 21.109710405659836 18.69419827429925 9 27.08204256315108 17.566896304451483 22.420745500442578 32.930315631954855 10-11 22.88834771444817 28.79527558065331 28.48828804745202 19.828088657446497 12-13 25.266917603673534 27.25209343553389 28.60242860472822 18.87856035606435 14-15 22.889704820730067 27.89500017108697 28.42130124576351 20.793993762419454 16-17 19.05169559115592 31.3536221106979 29.39168525021184 20.202997047934343 18-19 18.774480874725157 29.27775661185939 33.57265346743395 18.375109045981507 20-21 20.393513185939703 27.984202192018497 33.52090636083837 18.101378261203433 22-23 19.94903008038607 28.140505131924293 32.975389912585754 18.935074875103886 24-25 19.20508683845754 28.701955942392352 33.73196080122333 18.360996417926774 26-27 19.463302154084356 28.485985950648683 33.75308061556659 18.297631279700376 28-29 18.739761316134008 28.127284493357525 34.395269686517665 18.737684503990803 30-31 18.99032247548955 28.88532084661573 33.99925792287755 18.12509875501717 32-33 18.909391331653595 28.906907445759494 33.32874354418118 18.854957678405725 34-35 19.101230324525677 28.612680862381644 33.539657418095096 18.746431394997575 36-37 19.59084322999167 28.25420889798468 33.32410732671679 18.830840545306863 38-39 19.3328023965707 28.772582386792767 33.32615813784562 18.568457078790917 40-41 18.901042468419632 28.784414223572817 33.80197774214316 18.51256556586439 42-43 18.966917276685543 28.923687723209607 33.0558566691464 19.053538330958453 44-45 19.06279330128406 29.14851770382636 32.559540290386174 19.22914870450341 46-47 19.156835940095412 29.23679122427013 31.868953729419424 19.737419106215036 48-49 19.40284124318521 29.34635804453285 31.503103040380932 19.74769767190101 50-51 19.65310943917421 29.506986307771392 31.119771951960374 19.72013230109403 52-53 19.134122370735124 30.127853308344054 31.146196828367124 19.591827492553694 54-55 19.21212740852679 29.87502114428946 31.073190620289292 19.839660826894455 56-57 19.476963830515718 30.43349438051305 30.155681247426646 19.933860541544586 58-59 19.301277026151713 29.685335707238973 30.773606465349722 20.239780801259588 60-61 19.658429787526423 30.071497087951403 30.54083749342747 19.729235631094706 62-63 20.002831101350317 29.606429173793714 30.833807086180258 19.556932638675708 64-65 19.923211892256727 29.445576496089615 30.88163324266312 19.74957836899054 66-67 19.527171224663103 29.969313520006825 30.404213366003578 20.09930188932649 68-69 19.56464651350396 29.9992132265951 30.53637809797218 19.89976216192876 70-71 19.54124238704931 29.94642492347604 30.611722490819616 19.900610198655038 72-73 19.63570692849465 29.704342457176026 30.208503426375007 20.451447187954315 74-75 19.39284463041999 30.188014684305497 30.112981596437454 20.30615908883706 76-77 19.39257091497513 29.870181652650363 30.588988034915054 20.148259397459455 78-79 19.124485924640005 29.682693116347934 30.509221429557787 20.683599529454273 80-81 18.83773249181719 30.384710993780832 30.225426283887717 20.55213023051426 82-83 19.25177941043295 30.14024670392621 29.83735021339632 20.770623672244522 84-85 19.087602698994356 29.630092591054513 29.81894163142315 21.463363078527976 86-87 19.102931024638877 29.659934704639706 30.222512885193314 21.0146213855281 88-89 19.554306618443945 29.088245919269852 30.35741424341955 21.00003321886666 90-91 18.99889321956821 29.42299985256771 30.53956065177531 21.038546276088766 92-93 19.017729414134116 29.520232378033718 30.2950196105301 21.16701859730207 94-95 18.796437287585846 29.237386593916774 30.225619210555987 21.740556907941393 96-97 19.239451059203837 29.038639121315885 29.65419737326919 22.06771244621109 98-99 19.07686433752489 29.009057404275314 29.724123289714377 22.189954968485416 100-101 19.206524852294592 28.889693481923466 30.083495695311797 21.820285970470145 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6789.0 1 4600.5 2 2506.5 3 2541.0 4 2267.5 5 2378.0 6 3785.5 7 6882.5 8 10119.0 9 11344.0 10 10144.0 11 8346.5 12 7484.5 13 7109.5 14 6729.0 15 6310.0 16 6195.0 17 6217.5 18 6283.5 19 6540.0 20 7041.5 21 7775.0 22 8829.5 23 10240.0 24 11919.0 25 14630.0 26 19311.0 27 24315.0 28 28197.5 29 33032.0 30 40036.5 31 48705.5 32 59513.0 33 72402.0 34 85559.5 35 95773.5 36 105837.0 37 113875.5 38 113606.5 39 109412.0 40 104462.0 41 98880.0 42 92950.0 43 88937.5 44 86743.5 45 83812.5 46 79042.5 47 74431.5 48 76902.5 49 73300.5 50 60828.0 51 51234.5 52 43547.5 53 37783.0 54 33175.0 55 29392.0 56 26084.5 57 23299.5 58 20891.5 59 18970.0 60 17218.5 61 15195.0 62 12324.0 63 8970.0 64 6224.0 65 4334.5 66 3128.0 67 2362.5 68 1838.5 69 1352.0 70 1019.5 71 799.0 72 608.5 73 490.5 74 400.0 75 305.0 76 237.5 77 162.0 78 104.5 79 75.5 80 48.5 81 39.0 82 28.0 83 18.5 84 13.0 85 7.5 86 4.0 87 2.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.7633853851340127 2 3.9007563900308844 3 1.1799402695123806 4 1.0373893624700756 5 1.061879939813681 6 0.12282583967249759 7 1.9694402349106344 8 1.023300028634499 9 0.14238515017365105 10-11 1.1020345412450745 12-13 0.8897207121080833 14-15 0.6933195423618613 16-17 0.1381997892401415 18-19 0.15079731125783358 20-21 0.16484520587624674 22-23 0.6497876447316459 24-25 1.4942152924800667 26-27 0.22273579225360138 28-29 0.23359286715042807 30-31 0.20999323297583722 32-33 0.23433877305937037 34-35 0.7523082679940477 36-37 0.287235933768528 38-39 0.27907240798732624 40-41 0.26262103877343235 42-43 0.20647089951694306 44-45 0.17296729244028505 46-47 0.21038690553889008 48-49 0.6666948453343379 50-51 0.23974659089920203 52-53 0.2599896484835526 54-55 0.29363829282028264 56-57 0.21049050358179872 58-59 0.19745786978389032 60-61 0.18537833798074155 62-63 0.17472845916973212 64-65 0.1689062491582659 66-67 0.18471531050612616 68-69 0.19068255777766452 70-71 0.19795514038985187 72-73 0.2652938682804756 74-75 0.230754280774731 76-77 0.22795713361619743 78-79 0.2208917470898274 80-81 0.22934534739117335 82-83 0.24511296952187017 84-85 0.21403355664927462 86-87 0.20245129545208734 88-89 0.20317648175244793 90-91 0.2191305803603803 92-93 0.2083563838978805 94-95 0.21915129996896202 96-97 0.24225366353759137 98-99 0.25659163267614876 100-101 0.31245169741249385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2413173.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.2806552228249 #Duplication Level Percentage of deduplicated Percentage of total 1 76.81606440621694 35.55097792358437 2 11.208913768728964 10.3751174710584 3 4.350750871200733 6.040668030913385 4 2.2730833608193612 4.2079914925928374 5 1.3159175245333523 3.045076262730065 6 0.8669968275759271 2.4075108753794665 7 0.5993094362938364 1.941550337503041 8 0.4076965135629033 1.5094769423801988 9 0.32446473749397053 1.3514796583130553 >10 1.5770685383151992 13.16257954830587 >50 0.13823336518276225 4.484182599758079 >100 0.11131731432041184 9.714671448632389 >500 0.007714648299780747 2.363312404021581 >1k 0.002057239546608199 1.7237365812025105 >5k 2.057239546608199E-4 0.7090794105612249 >10k+ 2.057239546608199E-4 1.4125890130636607 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16590 0.6874766127418134 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13083 0.5421492781495566 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8826 0.3657425306847043 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6069 0.2514946089650431 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4317 0.17889310049465995 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3193 0.1323154204029301 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3045 0.1261824162627379 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2573 0.10662310576158443 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2442 0.10119456831317108 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.1439217163460724E-5 0.0 2 0.0 0.0 0.0 4.1439217163460724E-5 0.0 3 0.0 0.0 0.0 4.1439217163460724E-5 0.0 4 0.0 0.0 0.0 4.1439217163460724E-5 0.0 5 0.0 0.0 0.0 4.1439217163460724E-5 0.0 6 0.0 0.0 0.0 4.1439217163460724E-5 0.0 7 0.0 0.0 0.0 4.1439217163460724E-5 0.0 8 0.0 0.0 0.0 4.1439217163460724E-5 4.1439217163460724E-5 9 0.0 0.0 0.0 8.287843432692145E-5 4.1439217163460724E-5 10-11 0.0 0.0 0.0 1.2431765149038216E-4 4.1439217163460724E-5 12-13 0.0 0.0 0.0 1.2431765149038216E-4 4.1439217163460724E-5 14-15 4.1439217163460724E-5 0.0 0.0 1.4503726007211254E-4 4.1439217163460724E-5 16-17 4.1439217163460724E-5 0.0 0.0 1.8647647723557325E-4 4.1439217163460724E-5 18-19 4.1439217163460724E-5 0.0 0.0 2.2791569439903396E-4 4.1439217163460724E-5 20-21 4.1439217163460724E-5 0.0 0.0 2.693549115624947E-4 4.1439217163460724E-5 22-23 4.1439217163460724E-5 0.0 0.0 5.594294317067198E-4 4.1439217163460724E-5 24-25 4.1439217163460724E-5 2.0719608581730362E-5 0.0 0.00171972751228362 4.1439217163460724E-5 26-27 4.1439217163460724E-5 4.1439217163460724E-5 0.0 0.0030043432443509024 4.1439217163460724E-5 28-29 4.1439217163460724E-5 4.1439217163460724E-5 0.0 0.0061951629659373775 6.215882574519109E-5 30-31 4.1439217163460724E-5 4.1439217163460724E-5 0.0 0.014483006398629521 8.287843432692145E-5 32-33 8.287843432692145E-5 4.1439217163460724E-5 0.0 0.029007452014422504 8.287843432692145E-5 34-35 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.04910547233870095 8.287843432692145E-5 36-37 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.08105510877172917 8.287843432692145E-5 38-39 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.12711479864891576 8.287843432692145E-5 40-41 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.17885166127749647 8.287843432692145E-5 42-43 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.22917959052251952 8.287843432692145E-5 44-45 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.2910069025304029 8.287843432692145E-5 46-47 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.36222019722581017 8.287843432692145E-5 48-49 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.4429852314773951 8.287843432692145E-5 50-51 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.5303183816493886 8.287843432692145E-5 52-53 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.6159939631348437 8.287843432692145E-5 54-55 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.7076782311090004 8.287843432692145E-5 56-57 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.8131824780071715 8.287843432692145E-5 58-59 1.2431765149038216E-4 4.1439217163460724E-5 0.0 0.9413539766937555 8.287843432692145E-5 60-61 1.2431765149038216E-4 4.1439217163460724E-5 0.0 1.0891883839244016 8.287843432692145E-5 62-63 1.2431765149038216E-4 4.1439217163460724E-5 0.0 1.2299781242372594 8.287843432692145E-5 64-65 1.2431765149038216E-4 4.1439217163460724E-5 0.0 1.3750153843093718 8.287843432692145E-5 66-67 1.2431765149038216E-4 4.1439217163460724E-5 0.0 1.5388246097565321 8.287843432692145E-5 68-69 1.2431765149038216E-4 4.1439217163460724E-5 0.0 1.7239750320428746 8.287843432692145E-5 70-71 1.2431765149038216E-4 4.1439217163460724E-5 0.0 1.9387752142096732 8.287843432692145E-5 72-73 1.2431765149038216E-4 4.1439217163460724E-5 0.0 2.157470682789837 8.287843432692145E-5 74-75 1.2431765149038216E-4 4.1439217163460724E-5 0.0 2.3774507671020686 8.287843432692145E-5 76-77 1.2431765149038216E-4 4.1439217163460724E-5 0.0 2.6104634852122084 8.287843432692145E-5 78-79 1.2431765149038216E-4 4.1439217163460724E-5 0.0 2.8713233572561934 8.287843432692145E-5 80-81 1.2431765149038216E-4 4.1439217163460724E-5 0.0 3.1653760422481105 8.287843432692145E-5 82-83 1.2431765149038216E-4 4.1439217163460724E-5 0.0 3.4612106135780567 8.287843432692145E-5 84-85 1.2431765149038216E-4 4.1439217163460724E-5 0.0 3.7660167754238922 8.287843432692145E-5 86-87 1.2431765149038216E-4 8.287843432692145E-5 0.0 4.093800983186867 8.287843432692145E-5 88-89 1.2431765149038216E-4 8.287843432692145E-5 0.0 4.431592761894816 8.287843432692145E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 515 0.0 35.412155 9 GTATCAA 24285 0.0 29.214113 1 GGTATCA 16830 0.0 26.346727 1 GTACATG 15600 0.0 24.806458 1 GAGTACT 14090 0.0 24.37122 12-13 TATCAAC 29065 0.0 24.093075 2 TACATGG 16170 0.0 23.712547 2 ATCAACG 29520 0.0 23.525793 3 TCAACGC 29735 0.0 23.305164 4 CAACGCA 29610 0.0 23.150152 5 AACGCAG 30050 0.0 22.576094 6 CATGGGT 1855 0.0 22.491976 4 GTACTTT 15550 0.0 22.407743 14-15 AGTACTT 15020 0.0 22.287466 12-13 ACATGGG 16415 0.0 22.250303 3 CCTTTAG 985 0.0 21.996843 1 AACGCCG 360 0.0 21.645103 6 TTAGGCA 995 0.0 21.207127 4 CGTCACT 335 1.4551915E-11 21.057722 1 CATGGGA 11795 0.0 20.817295 4 >>END_MODULE