FastQCFastQC Report
Fri 27 May 2016
SRR939471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413173
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236120.9784627956636346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208840.8654166112417138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148160.6139634414938341No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85140.3528134949297046No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA58120.2408447301540337No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50440.20901941137249588No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA49920.20686457207999592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46590.1930653127645635No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40620.16832610011797744No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT34750.144001279643026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTC500.001636485137.9034848
CATTCCG4400.031.2273069
GGTATCA180050.028.8042371
GTATCAA254650.025.9135991
GTCGGGA9750.024.7897262
CGTCACT3200.023.702941
CGGGAGT10800.023.6896784
GTACATG170250.023.4453751
GAGTACT165350.022.80904412-13
TACATGG176100.022.3100932
ACATGGG175650.021.3092463
GTACTTT177300.021.2320714-15
ATCAACG308250.021.1343463
TATCAAC309800.021.1260552
TCAACGC309400.021.040484
AGTACTT174900.020.87281412-13
CAACGCA314400.020.6305165
AACGCAG319650.020.2916766
GGTCGGG11950.020.2317981
TCGGGAG12200.019.8061263
Produced by FastQC (version 0.11.5)