##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939471_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2413173 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.04083834851459 31.0 31.0 33.0 30.0 34.0 2 31.29997559230109 31.0 31.0 34.0 30.0 34.0 3 31.27515640196538 31.0 31.0 34.0 28.0 34.0 4 35.22126677200516 35.0 35.0 37.0 33.0 37.0 5 34.860012937323596 35.0 35.0 37.0 32.0 37.0 6 34.832964731496666 35.0 35.0 37.0 32.0 37.0 7 34.71084998879069 35.0 35.0 37.0 31.0 37.0 8 34.72870531868208 35.0 35.0 37.0 31.0 37.0 9 36.05208619522927 37.0 35.0 39.0 30.0 39.0 10-11 35.913366551009815 37.0 35.0 39.0 30.0 39.0 12-13 35.900693195224704 37.0 35.0 39.0 30.0 39.0 14-15 36.69991873769514 38.0 36.0 40.0 31.0 41.0 16-17 36.73138477846388 38.0 36.0 40.0 31.0 41.0 18-19 36.783745715702935 38.5 36.0 40.0 31.0 41.0 20-21 36.741473777470574 39.0 35.0 40.0 30.5 41.0 22-23 36.601954149163774 38.0 35.0 40.0 30.0 41.0 24-25 36.39403391302655 38.0 35.0 40.0 30.0 41.0 26-27 36.010942439684186 38.0 34.0 40.0 29.0 41.0 28-29 35.73338028396638 38.0 34.0 40.0 27.0 41.0 30-31 35.486251918117766 38.0 34.0 40.0 27.0 41.0 32-33 35.2211244282942 37.5 34.0 40.0 27.0 41.0 34-35 35.113640837188214 38.0 33.5 40.0 27.0 41.0 36-37 35.033963789583254 38.0 33.5 40.0 26.5 41.0 38-39 34.97318841210307 38.0 33.0 40.0 26.0 41.0 40-41 34.732999250364564 38.0 33.0 40.0 25.0 41.0 42-43 34.40824404218015 37.5 33.0 40.0 24.5 41.0 44-45 34.16525711169485 37.0 33.0 40.0 24.0 41.0 46-47 33.74576045729005 37.0 33.0 40.0 22.5 41.0 48-49 33.39394647627833 37.0 32.5 40.0 21.0 41.0 50-51 33.15576359423879 37.0 32.0 40.0 19.5 41.0 52-53 32.80753949260993 37.0 31.0 40.0 18.0 41.0 54-55 32.624790680154305 37.0 31.0 40.0 16.5 41.0 56-57 32.29142067311378 36.0 31.0 39.0 14.0 41.0 58-59 31.93846566325746 36.0 30.5 39.0 11.5 40.0 60-61 31.71394011121457 36.0 30.0 39.0 10.0 40.0 62-63 31.36290290832858 35.0 29.5 39.0 8.0 40.0 64-65 30.972704402046602 35.0 29.0 38.5 7.5 40.0 66-67 29.413205352455044 34.0 25.5 37.5 4.5 40.0 68-69 25.92703817753638 30.5 17.0 36.0 2.0 39.0 70-71 25.40460878685449 30.5 15.5 35.5 2.0 39.0 72-73 27.125063557399322 31.5 22.5 36.0 2.0 39.0 74-75 27.664076922790038 32.0 24.5 36.0 2.0 38.5 76-77 27.589985260070456 31.5 25.5 35.0 2.0 37.5 78-79 28.379962812446518 33.0 26.5 35.0 2.0 38.0 80-81 28.221333281948702 33.0 26.5 35.0 2.0 37.0 82-83 27.809439480716883 33.0 26.0 35.0 2.0 37.0 84-85 27.439572090355725 33.0 26.0 35.0 2.0 36.0 86-87 26.976654802618793 32.5 25.5 35.0 2.0 36.0 88-89 26.470826169528664 32.0 25.0 35.0 2.0 35.5 90-91 26.01325267604105 32.0 24.0 34.0 2.0 35.0 92-93 25.503207602604537 32.0 21.5 34.0 2.0 35.0 94-95 24.220332524854207 31.0 13.0 34.0 2.0 35.0 96-97 23.44271069666369 31.0 4.5 34.0 2.0 35.0 98-99 23.139160971882248 31.0 2.0 34.0 2.0 35.0 100-101 21.906430662037078 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37605339798416537 1101 2 0.2495813902021773 1101 3 0.2651208742969935 1101 4 0.23664974430917596 1101 5 0.43718420238405287 1101 6 0.5075009018888537 1101 7 0.475109706957241 1101 8 0.4535523063692608 1101 9 0.5171714201415583 1101 10-11 0.7083554350356991 1101 12-13 0.6048026167666976 1101 14-15 0.6367257769226882 1101 16-17 0.615807351997681 1101 18-19 0.7496710147947141 1101 20-21 0.7396412031347879 1101 22-23 0.8129951308438947 1101 24-25 0.7299736261938321 1101 26-27 0.8509633807229093 1101 28-29 0.7191531715969148 1101 30-31 0.916454296444492 1101 32-33 0.7204779416158473 1101 34-35 0.6926288893327879 1101 36-37 0.7860487678080403 1101 38-39 0.7667765304754113 1101 40-41 0.8550816943474615 1101 42-43 0.7756955030876398 1101 44-45 0.49516167275776723 1101 46-47 0.4087859829282223 1101 48-49 0.2816100110596338 1101 50-51 0.3328596328712976 1101 52-53 0.45889950172372806 1101 54-55 0.32895447761777774 1101 56-57 0.3838623920721034 1101 58-59 0.36152023102076214 1101 60-61 0.10529810234276482 1101 62-63 -0.012705743832047034 1101 64-65 -0.08330085685228283 1101 66-67 -3.066293032537015 1101 68-69 -8.141760994158133 1101 70-71 -11.594211101850089 1101 72-73 -11.489736724206944 1101 74-75 -9.801999595345965 1101 76-77 -4.592813364205551 1101 78-79 -1.538418821887383 1101 80-81 -0.9032259206669266 1101 82-83 -0.7066558493223489 1101 84-85 -0.729378901068106 1101 86-87 -0.5461340451896781 1101 88-89 -0.5834777063018386 1101 90-91 -0.5869262064983509 1101 92-93 -1.0319204911770328 1101 94-95 -4.503501767971812 1101 96-97 -1.6812775778847495 1101 98-99 -0.9225056637231681 1101 100-101 -0.7161508214269006 1102 1 0.37916573971092404 1102 2 0.2740142938879906 1102 3 0.43411801529294536 1102 4 0.23970827406565576 1102 5 0.44998302292940195 1102 6 0.49237730697693394 1102 7 0.43744408319464867 1102 8 0.49599828085298014 1102 9 0.559776237188153 1102 10-11 0.593776378362783 1102 12-13 0.6184157485731205 1102 14-15 0.7060081213611085 1102 16-17 0.7215867738140034 1102 18-19 0.719032715680882 1102 20-21 0.9430892699640694 1102 22-23 0.8070731397232009 1102 24-25 0.7797802515819612 1102 26-27 0.8675242577024278 1102 28-29 0.7674168359878664 1102 30-31 0.6762982806309097 1102 32-33 0.5107292548629445 1102 34-35 0.6851701774584456 1102 36-37 0.8040374449266565 1102 38-39 0.7546514941482769 1102 40-41 0.9617188660032738 1102 42-43 1.0251051517359926 1102 44-45 0.720706766135315 1102 46-47 0.8372438599066001 1102 48-49 0.776231164253403 1102 50-51 0.7323862820339357 1102 52-53 0.834953353242561 1102 54-55 0.7038360548142109 1102 56-57 0.5969027667719828 1102 58-59 0.5813482888089823 1102 60-61 0.4695297719515281 1102 62-63 0.61329960397563 1102 64-65 0.6328965948267573 1102 66-67 -2.132543104867114 1102 68-69 -7.581939993518063 1102 70-71 -11.23190791062196 1102 72-73 -8.907312669996895 1102 74-75 -6.943083802813156 1102 76-77 -3.492160505063733 1102 78-79 -0.684907566514088 1102 80-81 -0.0477807570906954 1102 82-83 0.13411145102348954 1102 84-85 0.04628369528490239 1102 86-87 -0.019796570549786452 1102 88-89 0.039107744918826626 1102 90-91 0.06413266558006114 1102 92-93 -0.3153385756621496 1102 94-95 -3.6283076923455724 1102 96-97 -0.6855853719813503 1102 98-99 0.001008724738635891 1102 100-101 0.21534816125131684 1103 1 0.31127980949617395 1103 2 0.25381623527001906 1103 3 0.276964050159787 1103 4 0.1732132834675113 1103 5 0.4006605060478492 1103 6 0.43112424046377384 1103 7 0.4676831570571949 1103 8 0.41806652030397373 1103 9 0.5226259563752294 1103 10-11 0.6536497663561818 1103 12-13 0.4551971779837558 1103 14-15 0.544015301747109 1103 16-17 0.563296525178302 1103 18-19 0.5625763172499134 1103 20-21 0.6814165606187217 1103 22-23 0.4973261090026213 1103 24-25 0.740579519191293 1103 26-27 0.694747345777877 1103 28-29 0.7715304758322006 1103 30-31 0.6773973636825019 1103 32-33 0.7044704459670257 1103 34-35 0.7920062224121196 1103 36-37 0.5692427991821205 1103 38-39 0.7175238131917752 1103 40-41 0.791380486352395 1103 42-43 0.7104247509838402 1103 44-45 0.4730300469075459 1103 46-47 0.48130419826139814 1103 48-49 0.4741078960935994 1103 50-51 0.41740462752563445 1103 52-53 0.43197857530362427 1103 54-55 0.47999760200504227 1103 56-57 0.4746025176609905 1103 58-59 0.3452045636630032 1103 60-61 0.3758181858827321 1103 62-63 0.35929950705253333 1103 64-65 0.39581462615714713 1103 66-67 -2.569607086846869 1103 68-69 -7.591533205422085 1103 70-71 -11.451233692444966 1103 72-73 -9.141297060408096 1103 74-75 -7.273265297522617 1103 76-77 -3.8104394863895727 1103 78-79 -1.0567532556264254 1103 80-81 -0.516301719971942 1103 82-83 -0.3135310500024353 1103 84-85 -0.3181107202051514 1103 86-87 -0.3238570580170368 1103 88-89 -0.18890515765761862 1103 90-91 -0.4199642581368046 1103 92-93 -0.3507612488943188 1103 94-95 0.46975409426394066 1103 96-97 0.5301488965770176 1103 98-99 0.13685086994496842 1103 100-101 0.08028730317748511 1104 1 0.41689014855734285 1104 2 0.26206412563919557 1104 3 0.2639732608670826 1104 4 0.09043636501255037 1104 5 0.34363954366527594 1104 6 0.3614626862986867 1104 7 0.4318598520614074 1104 8 0.3321290768604186 1104 9 0.54590157297622 1104 10-11 0.6946375043155442 1104 12-13 0.4857578680266883 1104 14-15 0.6099546877160407 1104 16-17 0.614453413662865 1104 18-19 0.7832705770270962 1104 20-21 0.8235723173711094 1104 22-23 0.618376056035622 1104 24-25 0.7954388188204007 1104 26-27 0.9707884971524763 1104 28-29 0.8163798878864768 1104 30-31 0.8689006551168106 1104 32-33 0.6662408527390795 1104 34-35 0.692228436456702 1104 36-37 0.9261741459916735 1104 38-39 0.9736053654746684 1104 40-41 1.1150978163331686 1104 42-43 1.0927125391351566 1104 44-45 0.844129009526597 1104 46-47 0.8594446645640161 1104 48-49 0.9246096950410134 1104 50-51 0.7637926463669302 1104 52-53 0.7894133498862246 1104 54-55 0.6971606281458804 1104 56-57 0.7190194161839472 1104 58-59 0.8171593534962369 1104 60-61 0.7694401873354053 1104 62-63 0.7400491107049625 1104 64-65 0.5573138937888835 1104 66-67 -2.373686058346042 1104 68-69 -7.186854946745271 1104 70-71 -8.627668235372361 1104 72-73 -7.905756507476088 1104 74-75 -6.573362091993776 1104 76-77 -3.3179120255960086 1104 78-79 -0.39449492508823525 1104 80-81 0.4026099108082768 1104 82-83 0.4952454244467184 1104 84-85 0.4483856638019539 1104 86-87 0.5012544260482201 1104 88-89 0.49447014307185455 1104 90-91 0.5466909665325481 1104 92-93 0.6340083265733973 1104 94-95 1.3948747178507865 1104 96-97 1.4962492648122634 1104 98-99 1.3955067208072798 1104 100-101 1.419231287260839 1105 1 0.44463518286877957 1105 2 0.4045476928335674 1105 3 0.48449428326779653 1105 4 0.2652329041161181 1105 5 0.36475514625724514 1105 6 0.4374680716944326 1105 7 0.5566195286269888 1105 8 0.6095507424735871 1105 9 0.7639657400800246 1105 10-11 0.8241932689114932 1105 12-13 0.8528497483506925 1105 14-15 1.08183176758169 1105 16-17 1.0272151107973855 1105 18-19 1.0335302428386086 1105 20-21 1.0335706385423933 1105 22-23 1.0387049541145856 1105 24-25 1.1137818596870304 1105 26-27 1.187966574278228 1105 28-29 1.2056243910666922 1105 30-31 1.0678646355778696 1105 32-33 1.1451383193208287 1105 34-35 1.1829396892691904 1105 36-37 1.1245252942268777 1105 38-39 1.0413449092395197 1105 40-41 1.1228621004172723 1105 42-43 1.265911502443494 1105 44-45 1.0766578393770132 1105 46-47 1.1847729173992079 1105 48-49 1.1295265721089294 1105 50-51 1.1370466794392584 1105 52-53 1.4212424038991003 1105 54-55 1.4533719209873794 1105 56-57 1.441503577839022 1105 58-59 1.5488642279544962 1105 60-61 1.62848728176683 1105 62-63 1.5648277114340878 1105 64-65 1.5839627604898787 1105 66-67 2.329745339096643 1105 68-69 -4.840263521676594 1105 70-71 -4.4437327591525815 1105 72-73 -0.7433078730628466 1105 74-75 1.488425750060106 1105 76-77 1.633099558322126 1105 78-79 1.5422194308688582 1105 80-81 1.5035681575614461 1105 82-83 1.6082807826107874 1105 84-85 1.3574149542847316 1105 86-87 1.572026167477528 1105 88-89 1.5090566851985052 1105 90-91 1.6350644204866356 1105 92-93 1.5507914458012984 1105 94-95 2.369927606929796 1105 96-97 2.3738618299788925 1105 98-99 2.0234062103477584 1105 100-101 1.8082512246960931 1106 1 0.11861853853250537 1106 2 0.10535833746040524 1106 3 0.14197535624195723 1106 4 0.031191406272604638 1106 5 0.2001134279958734 1106 6 0.20365594342943183 1106 7 0.21630345940227613 1106 8 0.22668522231239763 1106 9 0.38040433345604185 1106 10-11 0.33109776282358894 1106 12-13 0.20627458982446711 1106 14-15 0.26745830753499433 1106 16-17 0.2787617045197308 1106 18-19 0.20684192571866333 1106 20-21 0.33847508255963277 1106 22-23 0.32822505366949173 1106 24-25 0.3935666661170174 1106 26-27 0.49613267424697227 1106 28-29 0.5801493260199493 1106 30-31 0.4916991871772254 1106 32-33 0.4571899810395905 1106 34-35 0.6653089023833161 1106 36-37 0.5397091787760573 1106 38-39 0.49514829123012305 1106 40-41 0.5638155764009696 1106 42-43 0.6862427749236915 1106 44-45 0.4787939261236147 1106 46-47 0.413631147041734 1106 48-49 0.40322039087027406 1106 50-51 0.5211068878529943 1106 52-53 0.5205745466042586 1106 54-55 0.5652648730457628 1106 56-57 0.5239622348163024 1106 58-59 0.624735516841298 1106 60-61 0.5444204696224233 1106 62-63 0.6218015445181919 1106 64-65 0.5771222493904808 1106 66-67 1.278822064174527 1106 68-69 -5.627167516640149 1106 70-71 -6.05805749834191 1106 72-73 -2.227804147825001 1106 74-75 0.13626559638395364 1106 76-77 0.5220535714092556 1106 78-79 0.27069992711720303 1106 80-81 0.300997178462989 1106 82-83 0.3288267497124693 1106 84-85 0.37329413829841585 1106 86-87 0.41046142083332526 1106 88-89 0.43390586332753855 1106 90-91 0.3329418347005024 1106 92-93 0.30357447802897397 1106 94-95 1.305257859500479 1106 96-97 1.3645345006596514 1106 98-99 1.0423741059442442 1106 100-101 0.8599282054117303 1107 1 -0.037314922269445105 1107 2 -0.04878940127829168 1107 3 -0.12707299907898673 1107 4 -0.11823520272830734 1107 5 -0.001701185017829232 1107 6 0.0703782142871745 1107 7 0.01844313190375857 1107 8 0.07859630787171312 1107 9 0.049155213613765625 1107 10-11 0.0036848240332005844 1107 12-13 0.06123526764391585 1107 14-15 0.048735737605099416 1107 16-17 -0.03643326584163731 1107 18-19 0.07789217709895979 1107 20-21 0.15309818631880034 1107 22-23 0.11030460686151145 1107 24-25 0.2660894848865425 1107 26-27 0.4081852093189937 1107 28-29 0.32331480147933433 1107 30-31 0.1967274465918294 1107 32-33 0.18919491137464917 1107 34-35 0.253955920300065 1107 36-37 0.30251592735364596 1107 38-39 0.12674236030997577 1107 40-41 0.01070159652010716 1107 42-43 0.19973528432776533 1107 44-45 -0.012338729796773862 1107 46-47 -0.15215231310690314 1107 48-49 -0.027586508695193857 1107 50-51 0.022481458299729695 1107 52-53 0.11161472244706516 1107 54-55 -0.0203967224606032 1107 56-57 0.033560200544954455 1107 58-59 0.007016798554200676 1107 60-61 -0.06791945055725535 1107 62-63 0.08083061984807927 1107 64-65 -0.004642552140307288 1107 66-67 0.9033104112492119 1107 68-69 -5.4405426474842145 1107 70-71 -5.319961441012971 1107 72-73 -1.9756220825721051 1107 74-75 0.07878051732583202 1107 76-77 -0.003534373950554226 1107 78-79 0.15118452562736096 1107 80-81 0.07783483890576193 1107 82-83 0.24013777458626961 1107 84-85 0.33898301324996183 1107 86-87 0.3950489698499453 1107 88-89 0.15501563877661795 1107 90-91 0.2511117744650484 1107 92-93 0.39247541691227283 1107 94-95 1.0372230103359215 1107 96-97 1.2073874021676438 1107 98-99 0.8355364600195365 1107 100-101 0.6316227685413622 1108 1 0.06358225955808194 1108 2 0.09975029273840263 1108 3 0.10331579210212283 1108 4 0.04845698089255279 1108 5 0.09428487657824292 1108 6 0.24528928734443411 1108 7 0.15282856428175506 1108 8 0.07398924680871488 1108 9 0.11061090367297055 1108 10-11 0.3019045411623935 1108 12-13 0.27801065654874435 1108 14-15 0.237548189993106 1108 16-17 0.16601267359612137 1108 18-19 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78-79 1.1094453828954727 2316 80-81 0.6502826207846191 2316 82-83 0.8714149089180054 2316 84-85 1.229392131799596 2316 86-87 1.0582650687285131 2316 88-89 1.298532389586228 2316 90-91 1.1989007241631278 2316 92-93 1.5447882872472896 2316 94-95 2.240636809778742 2316 96-97 2.3831471634562327 2316 98-99 1.6743709034740668 2316 100-101 1.3384022857447029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 23.0 4 74.0 5 209.0 6 515.0 7 1194.0 8 2393.0 9 4156.0 10 6849.0 11 10172.0 12 13464.0 13 16728.0 14 20646.0 15 26473.0 16 32398.0 17 36579.0 18 31324.0 19 23706.0 20 22506.0 21 23034.0 22 24599.0 23 28634.0 24 33797.0 25 41665.0 26 52208.0 27 65247.0 28 79669.0 29 95820.0 30 113251.0 31 132197.0 32 154785.0 33 181079.0 34 213029.0 35 250686.0 36 272382.0 37 258420.0 38 133336.0 39 9918.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.80583087648007 23.044185226219092 16.545202162445356 20.604781734855486 2 14.575854601220636 23.299325437533028 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19.249354447313568 46-47 18.956668007914747 29.50383500159705 31.77843273087788 19.76106425961032 48-49 19.006102551572596 29.549400136312858 31.626781535622833 19.817715776491713 50-51 19.403909130562024 29.700693515278516 31.122651838820385 19.77274551533908 52-53 19.170299211671036 30.146854211187897 31.13588379394875 19.546962783192324 54-55 19.217202487247476 29.883356627175985 31.066418447811117 19.833022437765425 56-57 19.476713998877823 30.402559740792462 30.165356320204715 19.955369940125 58-59 19.139609982553388 29.789440811144114 30.86734255101823 20.20360665528427 60-61 19.601619762729875 30.14866474425868 30.57298008326751 19.67673540974394 62-63 19.985417231287933 29.639218406694727 30.93434563192412 19.441018730093216 64-65 19.84398637662535 29.493483063091926 30.937397497822783 19.725133062459946 66-67 19.30045806907005 29.988327237603645 30.79104548715944 19.920169206166864 68-69 19.50488919052964 29.842929859824064 30.854744315957085 19.797436633689212 70-71 19.14991085604322 29.801459030875304 31.349226335785254 19.699403777296222 72-73 19.67941080230402 29.775009998286006 30.217303007796065 20.328276191613906 74-75 19.16719508126242 30.131272295084415 30.528184970560947 20.17334765309222 76-77 19.141536875780595 30.02890596846392 30.788472509489402 20.041084646266086 78-79 18.947020519382292 29.877691841460447 30.72820236534965 20.447085273807613 80-81 18.676505294032694 30.52980937918477 30.42579558637569 20.367889740406852 82-83 19.035125123644264 30.397178320824906 29.99954831253292 20.56814824299791 84-85 18.828654224127323 29.84616105020237 30.05621644200395 21.26896828366636 86-87 18.780604188556094 29.885455669211936 30.399136737333727 20.934803404898243 88-89 19.305875215589225 29.29504291893514 30.5786120570838 20.820469808391838 90-91 18.745051723155502 29.56821913834455 30.83866623153947 20.848062906960482 92-93 18.757431992413178 29.814786305677917 30.54265795295449 20.885123748954417 94-95 18.308039227493296 29.467962068185074 30.694703707010575 21.52929499731105 96-97 18.592301695972516 29.252089285143246 30.268730057673114 21.886878961211124 98-99 18.656257893976655 29.158792980792526 30.23514025919286 21.94980886603796 100-101 18.677224776677946 29.02598223369713 30.585890148964257 21.710902840660673 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6590.0 1 4408.0 2 2373.0 3 2582.5 4 2347.5 5 2457.0 6 4436.5 7 8613.0 8 12070.5 9 12915.5 10 11357.0 11 8947.5 12 7807.5 13 7170.0 14 6610.5 15 6097.0 16 5738.0 17 5774.5 18 6080.5 19 6411.0 20 6817.0 21 7482.0 22 8531.0 23 9910.0 24 11451.0 25 14335.0 26 19600.5 27 24732.0 28 28208.0 29 33109.0 30 40071.0 31 48199.0 32 60283.5 33 74709.0 34 87770.0 35 97325.5 36 108382.0 37 116928.5 38 114378.0 39 109004.0 40 103639.0 41 96765.5 42 89907.5 43 86584.5 44 85762.5 45 82930.5 46 77387.5 47 73188.5 48 78778.0 49 74687.5 50 59895.5 51 50501.0 52 42369.5 53 36626.5 54 31975.5 55 28261.0 56 25497.5 57 22975.0 58 20478.5 59 18976.0 60 17983.5 61 16188.5 62 13045.0 63 8855.5 64 5678.0 65 3997.5 66 2877.0 67 2146.5 68 1536.0 69 1077.0 70 852.0 71 694.0 72 555.5 73 500.0 74 397.0 75 292.0 76 211.5 77 136.5 78 94.0 79 54.5 80 30.5 81 25.0 82 22.5 83 13.0 84 5.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022128541965288025 2 0.02063673014740344 3 0.0 4 0.0 5 1.657568686538429E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.3511178021633757E-4 18-19 9.945412119230573E-4 20-21 8.287843432692145E-5 22-23 0.0 24-25 0.0 26-27 3.9367256305287686E-4 28-29 0.019207077155264044 30-31 0.0043303981935816456 32-33 0.008308563041273875 34-35 0.009385982687523853 36-37 0.10490337824930081 38-39 0.10745189010485366 40-41 0.12147906511468512 42-43 0.10738973127910846 44-45 0.10144320361615185 46-47 0.10289357621687298 48-49 0.10533849002951716 50-51 0.1029142958254547 52-53 0.07749133609567155 54-55 0.06595051411564773 56-57 0.037191697404206 58-59 0.015891939782187187 60-61 0.009406702296105584 62-63 0.0021134000753364967 64-65 0.004744790365216253 66-67 0.4025612751344392 68-69 0.781564355311451 70-71 0.857563879589238 72-73 0.269790023342711 74-75 0.14292385999677604 76-77 0.014483006398629521 78-79 0.008722955212908482 80-81 0.0013053353406490128 82-83 0.0 84-85 0.0 86-87 1.035980429086518E-4 88-89 7.873451261057537E-4 90-91 0.0035430530674758916 92-93 0.00171972751228362 94-95 0.214157874300765 96-97 0.21929633722903413 98-99 0.013944296575504533 100-101 0.007376180655096008 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2413173.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.58062550806574 #Duplication Level Percentage of deduplicated Percentage of total 1 67.72345713567614 21.3874913791333 2 13.995394230679285 8.839666080736526 3 5.951926698476149 5.638967043481001 4 3.254567250846475 4.111250781591902 5 1.9843514103150661 3.13335293827811 6 1.352304439785965 2.5623972051465116 7 0.9557898629442307 2.112910920823306 8 0.7123043253512281 1.7996012917354043 9 0.5400338873979285 1.534914716362103 >10 3.005291864496986 17.19561840663766 >50 0.2637789384811506 5.8472892327003905 >100 0.23444086448646692 14.869800721647158 >500 0.01792953402256635 3.830137909145577 >1k 0.007626742084225985 3.8118412065721556 >5k 4.014074781171571E-4 0.8184922144276365 >10k+ 4.014074781171571E-4 2.506267951581413 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23612 0.9784627956636346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20884 0.8654166112417138 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14816 0.6139634414938341 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8514 0.3528134949297046 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5812 0.2408447301540337 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5044 0.20901941137249588 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4992 0.20686457207999592 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4659 0.1930653127645635 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4062 0.16832610011797744 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3475 0.144001279643026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.0719608581730362E-5 0.0 18-19 0.0 0.0 0.0 6.215882574519109E-5 0.0 20-21 0.0 0.0 0.0 1.4503726007211254E-4 0.0 22-23 0.0 0.0 0.0 5.17990214543259E-4 0.0 24-25 0.0 0.0 0.0 0.0016782882951201592 0.0 26-27 0.0 0.0 0.0 0.0032322589387499363 0.0 28-29 0.0 0.0 0.0 0.006858190440552749 0.0 30-31 0.0 0.0 0.0 0.016264892736658335 0.0 32-33 0.0 0.0 0.0 0.03284057960204262 0.0 34-35 0.0 0.0 0.0 0.056005101996417166 0.0 36-37 0.0 0.0 0.0 0.09197434249430107 0.0 38-39 0.0 0.0 0.0 0.1444778306404058 0.0 40-41 0.0 0.0 0.0 0.2029485660580489 0.0 42-43 0.0 0.0 0.0 0.25988605044064395 0.0 44-45 0.0 0.0 0.0 0.3301462431412916 0.0 46-47 0.0 0.0 0.0 0.4108283989585496 0.0 48-49 0.0 0.0 0.0 0.49988127664282667 0.0 50-51 0.0 0.0 0.0 0.595750905550493 0.0 52-53 0.0 0.0 0.0 0.6903566383346739 0.0 54-55 0.0 0.0 0.0 0.7932502145515469 0.0 56-57 0.0 0.0 0.0 0.9074566970540445 0.0 58-59 0.0 0.0 0.0 1.026760203267648 0.0 60-61 0.0 0.0 0.0 1.1532326940505302 0.0 62-63 0.0 0.0 0.0 1.2718317335723546 0.0 64-65 0.0 0.0 0.0 1.3983249439638186 0.0 66-67 0.0 0.0 0.0 1.52730450738509 0.0 68-69 0.0 0.0 0.0 1.6799873030238612 0.0 70-71 0.0 0.0 0.0 1.8872041084497464 0.0 72-73 0.0 0.0 0.0 2.1142081400711845 0.0 74-75 0.0 0.0 0.0 2.3588238389870932 0.0 76-77 0.0 0.0 0.0 2.6239726700074963 0.0 78-79 0.0 0.0 0.0 2.9220242394556877 0.0 80-81 0.0 0.0 0.0 3.2579719729998637 0.0 82-83 0.0 0.0 0.0 3.5996175989040156 0.0 84-85 0.0 0.0 0.0 3.9436459797950665 0.0 86-87 0.0 0.0 0.0 4.306839998624218 0.0 88-89 0.0 0.0 0.0 4.683978314028874 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCGTC 50 0.0016364851 37.903484 8 CATTCCG 440 0.0 31.227306 9 GGTATCA 18005 0.0 28.804237 1 GTATCAA 25465 0.0 25.913599 1 GTCGGGA 975 0.0 24.789726 2 CGTCACT 320 0.0 23.70294 1 CGGGAGT 1080 0.0 23.689678 4 GTACATG 17025 0.0 23.445375 1 GAGTACT 16535 0.0 22.809044 12-13 TACATGG 17610 0.0 22.310093 2 ACATGGG 17565 0.0 21.309246 3 GTACTTT 17730 0.0 21.23207 14-15 ATCAACG 30825 0.0 21.134346 3 TATCAAC 30980 0.0 21.126055 2 TCAACGC 30940 0.0 21.04048 4 AGTACTT 17490 0.0 20.872814 12-13 CAACGCA 31440 0.0 20.630516 5 AACGCAG 31965 0.0 20.291676 6 GGTCGGG 1195 0.0 20.231798 1 TCGGGAG 1220 0.0 19.806126 3 >>END_MODULE