FastQCFastQC Report
Fri 27 May 2016
SRR939470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430445
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168710.6941527168892939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123120.5065738990184925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91480.3763919776008097No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59210.24361793827879255No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42000.17280786028895942No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30330.12479196196581285No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.12380448847844736No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.10911582035388581No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24480.10072229571127922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG6200.037.6254589
GTATCAA238100.029.3664381
GGTATCA165650.027.9976121
TTAGGCA10050.026.0999854
GTACATG150950.025.8941211
GAGTACT139800.024.82672112-13
TACATGG157750.024.2942772
TATCAAC288500.023.8635032
ATCAACG292200.023.8245943
TCAACGC295750.023.5757884
CAACGCA295600.023.4363025
TAGGCAT11850.023.4013025
AACGCAG299100.022.9607266
ACATGGG161550.022.9029883
GTACTTT156700.022.67490814-15
GTATAGG12600.022.5026911
AGTACTT150250.022.1228212-13
GGCATTC11200.021.6976057
CATGGGA119850.021.448374
CCTTTAG11150.021.342241
Produced by FastQC (version 0.11.5)