FastQCFastQC Report
Fri 27 May 2016
SRR939470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430445
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231830.9538582440664158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201100.8274204929549939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150520.6193104554927183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86560.35614877110981735No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA55470.2282298097673471No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA47700.19626035561388966No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47540.19560203995564596No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46250.1902943699610565No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39780.16367373053082873No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT34500.14194931380878809No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG24390.10035199315351714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG5000.034.140969
GGTATCA185250.029.3475441
GTATCAA253500.024.6866381
GTACATG169150.024.056551
ACCGCGC1201.7317475E-423.7090028
CGTCACT3500.023.0624471
GAGTACT161300.022.97028512-13
GGTACTC5400.022.8501663
TACATGG175950.022.6629312
GGATATG10300.022.1273061
ACATGGG174600.021.5273593
AGTACTT169650.021.14106212-13
GTACTTT174600.021.071214-15
TCGACCG704.915821E-420.31949218-19
CATGGGA127100.020.278914
ATCAACG306800.020.2176193
GATTGGC11100.020.0778949
TCAACGC308300.020.0767944
TTAGGCA9700.020.0633034
GTGGTAC7100.020.0626091
Produced by FastQC (version 0.11.5)