##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939470_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2430445 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93709793885482 31.0 31.0 31.0 30.0 34.0 2 31.18641236481385 31.0 31.0 34.0 30.0 34.0 3 31.17632120866755 31.0 31.0 34.0 28.0 34.0 4 35.09066158666417 35.0 35.0 37.0 32.0 37.0 5 34.683460436257555 35.0 35.0 37.0 32.0 37.0 6 34.75123444472103 35.0 35.0 37.0 32.0 37.0 7 34.60213952588929 35.0 35.0 37.0 30.0 37.0 8 34.49733608454419 35.0 35.0 37.0 30.0 37.0 9 35.973496623046394 37.0 35.0 39.0 30.0 39.0 10-11 35.78869569152974 37.0 35.0 39.0 30.0 39.0 12-13 35.789831080316574 37.0 35.0 39.0 30.0 39.0 14-15 36.53523531698927 38.0 35.0 40.0 30.5 41.0 16-17 36.48348121434552 38.0 35.0 40.0 30.0 41.0 18-19 36.58690034129552 38.0 35.0 40.0 30.0 41.0 20-21 36.5224094764539 38.0 35.0 40.0 30.0 41.0 22-23 36.35494076187694 38.0 34.5 40.0 30.0 41.0 24-25 36.208209607705584 38.0 34.0 40.0 30.0 41.0 26-27 35.72670580901851 38.0 34.0 40.0 27.5 41.0 28-29 35.52923744417174 38.0 34.0 40.0 27.0 41.0 30-31 35.208751895229064 37.5 34.0 40.0 27.0 41.0 32-33 34.888345755612654 37.0 33.0 40.0 26.5 41.0 34-35 35.066914700805825 37.5 33.0 40.0 27.0 41.0 36-37 34.83770605794412 38.0 33.0 40.0 25.0 41.0 38-39 34.62808888907175 37.0 33.0 40.0 25.0 41.0 40-41 34.453029589231605 37.0 33.0 40.0 24.5 41.0 42-43 34.275905646908285 37.0 33.0 40.0 24.0 41.0 44-45 33.824189808862165 37.0 33.0 40.0 23.5 41.0 46-47 33.33257983620284 37.0 32.0 40.0 21.0 41.0 48-49 32.89907959242032 37.0 31.0 40.0 18.5 41.0 50-51 32.68923304168578 37.0 31.0 40.0 17.5 41.0 52-53 32.59466455731358 37.0 31.0 39.5 17.5 41.0 54-55 32.29685880569196 36.0 31.0 39.0 14.5 40.0 56-57 31.857579784771925 36.0 30.0 39.0 11.0 40.0 58-59 31.4691756036446 35.0 29.5 39.0 9.0 40.0 60-61 31.15364964029221 35.0 29.0 39.0 8.0 40.0 62-63 31.026382000004116 35.0 29.0 39.0 8.0 40.0 64-65 30.609723527995904 35.0 28.0 38.0 6.5 40.0 66-67 29.832712939399986 34.0 27.0 38.0 2.0 40.0 68-69 26.553153846312092 31.5 18.5 36.0 2.0 39.0 70-71 25.35523371234486 30.5 13.5 36.0 2.0 39.0 72-73 26.299785224516498 31.0 20.0 35.5 2.0 38.5 74-75 26.987543639127814 31.5 22.5 35.0 2.0 38.0 76-77 26.561186325960882 31.5 23.0 34.5 2.0 37.0 78-79 27.60108910096711 32.5 26.0 35.0 2.0 37.0 80-81 27.597417551106897 33.0 26.0 35.0 2.0 37.0 82-83 27.2713118379556 32.5 26.0 35.0 2.0 36.5 84-85 26.880687898718136 32.0 25.5 35.0 2.0 36.0 86-87 26.424975878902835 32.0 24.5 35.0 2.0 36.0 88-89 25.918399716924267 32.0 23.5 34.0 2.0 35.0 90-91 25.32118644939507 31.0 20.5 34.0 2.0 35.0 92-93 24.79489537924125 31.0 19.0 34.0 2.0 35.0 94-95 24.00879859449607 31.0 11.5 34.0 2.0 35.0 96-97 23.353888279718323 31.0 2.0 34.0 2.0 35.0 98-99 22.54227353427047 30.0 2.0 34.0 2.0 35.0 100-101 21.150184225522487 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.21220552912152968 1101 2 0.1669360378164022 1101 3 0.24027618974273324 1101 4 0.2755901055569865 1101 5 0.43277539831507994 1101 6 0.3299312473539473 1101 7 0.3626055266168038 1101 8 0.447644197328259 1101 9 0.46266958118541623 1101 10-11 0.5059099498059467 1101 12-13 0.5169374238361257 1101 14-15 0.47342897994938227 1101 16-17 0.5378178524899582 1101 18-19 0.42735671382881435 1101 20-21 0.5966078613702663 1101 22-23 0.6226984445150379 1101 24-25 0.5346226257689324 1101 26-27 0.5641806467936945 1101 28-29 0.5232503341110686 1101 30-31 0.24458555323242592 1101 32-33 0.763977114889066 1101 34-35 0.5854534974826038 1101 36-37 0.6274624966288584 1101 38-39 0.7181973673642474 1101 40-41 0.6730152833188114 1101 42-43 0.5704090763895664 1101 44-45 0.8296985919563582 1101 46-47 0.8480518923202922 1101 48-49 1.0204655929571587 1101 50-51 0.589800266583488 1101 52-53 0.27722107830584264 1101 54-55 0.2315194854031546 1101 56-57 0.579740727476775 1101 58-59 0.6080009984732229 1101 60-61 0.4574340699677677 1101 62-63 0.2564750749150768 1101 64-65 0.07312218384969071 1101 66-67 -0.08540701184790578 1101 68-69 -7.26758403069984 1101 70-71 -7.6137160778839075 1101 72-73 -4.668497780696722 1101 74-75 -2.7957895784694813 1101 76-77 -0.30439788635250764 1101 78-79 -0.13105414980048735 1101 80-81 -0.15490846344441067 1101 82-83 -0.32953739348668876 1101 84-85 -0.39001796976110725 1101 86-87 -0.5493019736807128 1101 88-89 -0.5100837915850391 1101 90-91 -0.4172919780525213 1101 92-93 -0.43133893435619086 1101 94-95 -0.654765073977174 1101 96-97 -0.5635089531364379 1101 98-99 -0.6927788341988865 1101 100-101 -0.7382450953222062 1102 1 0.2508299785176753 1102 2 0.2878662288115521 1102 3 0.31462885322603995 1102 4 0.2940663315341894 1102 5 0.46038058990064457 1102 6 0.38030276333446267 1102 7 0.38645151647184406 1102 8 0.5167860308382899 1102 9 0.5649954097321199 1102 10-11 0.571867839342417 1102 12-13 0.6029532987162085 1102 14-15 0.8227416865554815 1102 16-17 0.6691581344476418 1102 18-19 0.4971621283048222 1102 20-21 0.6736268964180638 1102 22-23 0.6408856852501117 1102 24-25 0.5905268288366727 1102 26-27 0.6215206804570457 1102 28-29 0.5987979539641515 1102 30-31 0.46258390836226226 1102 32-33 0.5816082784448184 1102 34-35 0.4952443047200319 1102 36-37 0.716978507783594 1102 38-39 0.6120055832896796 1102 40-41 0.669446690573217 1102 42-43 0.4053781576813904 1102 44-45 0.5931570703142199 1102 46-47 0.602041356725799 1102 48-49 0.390023941806227 1102 50-51 0.5449494697757089 1102 52-53 0.3665254967322227 1102 54-55 -0.01416304954793901 1102 56-57 -0.026248399912255138 1102 58-59 0.060404602506949345 1102 60-61 0.2466349739123288 1102 62-63 0.04001475637068097 1102 64-65 -0.19260373558932997 1102 66-67 -0.12816821078400764 1102 68-69 -7.043782832759469 1102 70-71 -7.894647392267302 1102 72-73 -1.6234062692352929 1102 74-75 -0.2690305982251573 1102 76-77 0.19217139127693983 1102 78-79 0.027894275828341364 1102 80-81 -0.12855607314869744 1102 82-83 -0.26567660171499696 1102 84-85 -0.5166348350100485 1102 86-87 -0.46109916189100986 1102 88-89 -0.44386579937343384 1102 90-91 -0.3899431391955446 1102 92-93 -0.30856480688098387 1102 94-95 -0.10689333619130537 1102 96-97 -0.42593243029112315 1102 98-99 -0.5133561791601373 1102 100-101 -0.6105275010836557 1103 1 0.3734154782273045 1103 2 0.24569873765181427 1103 3 0.3283985939539349 1103 4 0.33656070127114646 1103 5 0.3731524305101388 1103 6 0.4395720528059428 1103 7 0.5277413394252477 1103 8 0.5346011453448014 1103 9 0.5342361820256869 1103 10-11 0.533960598602917 1103 12-13 0.46341768287418716 1103 14-15 0.6573694363654354 1103 16-17 0.6503432015575257 1103 18-19 0.7129717584292621 1103 20-21 0.5212461746119956 1103 22-23 0.6726236149531246 1103 24-25 0.5799169659214698 1103 26-27 0.7613363011380514 1103 28-29 0.6746987573769303 1103 30-31 0.562726788179539 1103 32-33 0.6535984507390609 1103 34-35 0.6971718827833726 1103 36-37 0.709202881694317 1103 38-39 0.8018439137554125 1103 40-41 0.7527724952313193 1103 42-43 0.6034262764356129 1103 44-45 0.6072770491390429 1103 46-47 0.6101334192228833 1103 48-49 0.7171550648236504 1103 50-51 0.4560716796667137 1103 52-53 0.3909699191750562 1103 54-55 0.10640626062223646 1103 56-57 0.3378729866717123 1103 58-59 0.47618363320754753 1103 60-61 0.3233767200348794 1103 62-63 0.07062640141612775 1103 64-65 0.386583566608909 1103 66-67 0.1371328160757308 1103 68-69 -7.110113503932613 1103 70-71 -7.852196879641866 1103 72-73 -1.0811526108405793 1103 74-75 0.48154155530907516 1103 76-77 0.5777030152709699 1103 78-79 0.49008403124024724 1103 80-81 0.25263717752803316 1103 82-83 0.15983592316444017 1103 84-85 -0.1219390084434977 1103 86-87 0.049358400049616336 1103 88-89 0.3060628844769653 1103 90-91 0.12777923337672803 1103 92-93 0.01559865159027396 1103 94-95 0.3362596482425886 1103 96-97 0.18426339673952086 1103 98-99 0.5573084564143365 1103 100-101 0.3829996688223538 1104 1 0.3356871860376316 1104 2 0.33700740252929506 1104 3 0.2856105059746774 1104 4 0.32078388820045944 1104 5 0.564722478635673 1104 6 0.33990505415162886 1104 7 0.46164923946402325 1104 8 0.5152156012525353 1104 9 0.7346602645613629 1104 10-11 0.5035375044273991 1104 12-13 0.28022718025503224 1104 14-15 0.6063993988837808 1104 16-17 0.6059188453221154 1104 18-19 0.44006331859378633 1104 20-21 0.5493392477278434 1104 22-23 0.6170120100843093 1104 24-25 0.6003656814334093 1104 26-27 0.8276485013037913 1104 28-29 0.7612619249952104 1104 30-31 0.5980445459704811 1104 32-33 0.6927010197151944 1104 34-35 0.5859292099533917 1104 36-37 0.8765176591992443 1104 38-39 0.9052309479906953 1104 40-41 0.9448763338701198 1104 42-43 0.8534972863116153 1104 44-45 0.9835875766952853 1104 46-47 1.0166442037527688 1104 48-49 1.0245564378839944 1104 50-51 1.0986813306235206 1104 52-53 0.7475717848949301 1104 54-55 0.6921872552166661 1104 56-57 0.7547056725024248 1104 58-59 0.9369953049807407 1104 60-61 1.0202851294592747 1104 62-63 0.7706692810414602 1104 64-65 0.7072992153913233 1104 66-67 0.5673652327683705 1104 68-69 -6.307547720961299 1104 70-71 -3.690402245677248 1104 72-73 0.7936101447075465 1104 74-75 1.2282137466556016 1104 76-77 1.355008061229718 1104 78-79 0.8098535152402135 1104 80-81 0.5623172529475191 1104 82-83 0.5277196920098071 1104 84-85 0.5753252866656418 1104 86-87 0.5095074903781374 1104 88-89 0.46334375362568636 1104 90-91 0.4729199986716033 1104 92-93 0.42086858795508775 1104 94-95 0.6742423291315056 1104 96-97 0.6019167256125648 1104 98-99 0.5052847509760774 1104 100-101 0.730242600146525 1105 1 0.10670546416551474 1105 2 0.2102893593685593 1105 3 0.16533093616254035 1105 4 0.19054963081244125 1105 5 0.3113920341405887 1105 6 0.09066654942427022 1105 7 0.22928548492063783 1105 8 0.37499213965229217 1105 9 0.43526296089340377 1105 10-11 0.3211539140248192 1105 12-13 0.2706109350225461 1105 14-15 0.3886277391341366 1105 16-17 0.4565658828309438 1105 18-19 0.4138958232539025 1105 20-21 0.47513475533532556 1105 22-23 0.43085489961986667 1105 24-25 0.5187578188126096 1105 26-27 0.5082339393176554 1105 28-29 0.6050017502454068 1105 30-31 0.4415894493500474 1105 32-33 0.5583330589237079 1105 34-35 0.5676578808013986 1105 36-37 0.4922531362497935 1105 38-39 0.44991318200900565 1105 40-41 0.6246194015922057 1105 42-43 0.5067953060877031 1105 44-45 0.6260751652749121 1105 46-47 0.6787575632599712 1105 48-49 0.6548166948162688 1105 50-51 0.25202604498341685 1105 52-53 0.1836510087935892 1105 54-55 0.11917440860277395 1105 56-57 0.45726849539546777 1105 58-59 0.4129093421197467 1105 60-61 0.6272896158748864 1105 62-63 0.3557446460997191 1105 64-65 0.1854700577989732 1105 66-67 0.3485178045192683 1105 68-69 -2.7341115599284898 1105 70-71 -2.9457043969901164 1105 72-73 0.6632387173101506 1105 74-75 1.1207826585223764 1105 76-77 1.0304544801286966 1105 78-79 0.709267031267661 1105 80-81 0.6015841853374937 1105 82-83 0.6634367900721649 1105 84-85 0.5964874961786357 1105 86-87 0.5637462752729974 1105 88-89 0.4277840723442381 1105 90-91 0.4314159100938433 1105 92-93 0.4164149706190692 1105 94-95 0.47615307150067565 1105 96-97 0.45892177236317977 1105 98-99 0.39293668581285246 1105 100-101 0.37674771600512713 1106 1 0.012342321253196786 1106 2 0.08183647666536942 1106 3 0.03102508024094064 1106 4 0.11816959664074034 1106 5 0.2145755456296854 1106 6 0.1519424011739119 1106 7 0.2304875387609755 1106 8 0.2657196372604176 1106 9 0.1835000517874903 1106 10-11 0.34472487190870993 1106 12-13 0.1352281102107682 1106 14-15 0.1502196570394858 1106 16-17 0.14502497187049102 1106 18-19 0.18756768938573032 1106 20-21 0.30288312303938625 1106 22-23 0.21622526753325388 1106 24-25 0.130395068034602 1106 26-27 0.3380963679853366 1106 28-29 0.3786197347634612 1106 30-31 0.3202039031850745 1106 32-33 0.35474952620629097 1106 34-35 0.25858962731752655 1106 36-37 0.3209122580163424 1106 38-39 0.2484943486528195 1106 40-41 0.36118106176507325 1106 42-43 0.08260415682457989 1106 44-45 0.16615276659224776 1106 46-47 0.4333290975518196 1106 48-49 0.5186835814319579 1106 50-51 0.3337547971265096 1106 52-53 0.19531199246104336 1106 54-55 0.2482438709217547 1106 56-57 0.506423858716758 1106 58-59 0.6546919906185025 1106 60-61 0.8377206835269035 1106 62-63 0.28089247041764764 1106 64-65 0.43303853019994065 1106 66-67 0.5977059685493487 1106 68-69 -2.7776288131013125 1106 70-71 -2.879786986963566 1106 72-73 1.0023866949437519 1106 74-75 1.2641018543816749 1106 76-77 1.1666537108967923 1106 78-79 0.9025682420192176 1106 80-81 0.6434302239700962 1106 82-83 0.5442427735111259 1106 84-85 0.30183423283980204 1106 86-87 0.3855105406329429 1106 88-89 0.3846051455834285 1106 90-91 0.5872811190822702 1106 92-93 0.4687992504555538 1106 94-95 0.7101349006451017 1106 96-97 0.8342836489470109 1106 98-99 0.8064542633072911 1106 100-101 0.7991177256905502 1107 1 0.0019560932800821718 1107 2 0.03462046450118095 1107 3 0.16115402362779463 1107 4 -0.07377464080177276 1107 5 0.2813265185387763 1107 6 0.17338780159092693 1107 7 0.10453401257711192 1107 8 0.03556961731295871 1107 9 0.15948030003926306 1107 10-11 -0.027250829827849543 1107 12-13 0.15851432237645469 1107 14-15 0.1050676423461141 1107 16-17 0.001433751763450175 1107 18-19 0.20686553882381986 1107 20-21 0.1430806405216387 1107 22-23 0.3686438028628416 1107 24-25 0.2338621622543542 1107 26-27 0.43990707036171983 1107 28-29 0.31863923058202914 1107 30-31 0.27045456370943555 1107 32-33 0.33313438420296393 1107 34-35 0.05360464436696333 1107 36-37 0.252059650921062 1107 38-39 0.38331094493882745 1107 40-41 0.0809508756138797 1107 42-43 -0.0725938499349823 1107 44-45 0.30371270306147125 1107 46-47 0.31584232685727187 1107 48-49 0.289022838784291 1107 50-51 0.2752319629313007 1107 52-53 0.045376351244328816 1107 54-55 0.10729550096790774 1107 56-57 0.5410707134164596 1107 58-59 0.6635667281024169 1107 60-61 0.5029940389906216 1107 62-63 0.2973829972563884 1107 64-65 0.22278741803500068 1107 66-67 0.38995092334765147 1107 68-69 -2.2223122506883506 1107 70-71 -2.4839040672577326 1107 72-73 0.9778121780109963 1107 74-75 1.397228185780481 1107 76-77 1.121223560876846 1107 78-79 0.9385982460087128 1107 80-81 0.7040388849310375 1107 82-83 0.6581574435500421 1107 84-85 0.43961773139831806 1107 86-87 0.47140994522010615 1107 88-89 0.46025908351295897 1107 90-91 0.42658852824606086 1107 92-93 0.3410429256055352 1107 94-95 0.6349171722466949 1107 96-97 0.581800754834152 1107 98-99 0.7277762152978937 1107 100-101 1.0070270814252567 1108 1 -0.15161500681083595 1108 2 -0.05667618053890422 1108 3 -0.015502385113105532 1108 4 0.052847013624237604 1108 5 -0.1846952206244339 1108 6 -0.07923822237975031 1108 7 -0.02076818410667869 1108 8 0.06537766191910777 1108 9 0.19788389337909962 1108 10-11 0.1455501116296034 1108 12-13 -0.027824291702884807 1108 14-15 -0.14349099594817005 1108 16-17 0.0845623789152441 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0.1452972040053666 2316 96-97 0.5338368623735228 2316 98-99 0.5937309382114115 2316 100-101 0.8011004433967202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 24.0 4 76.0 5 235.0 6 607.0 7 1319.0 8 2636.0 9 4650.0 10 7519.0 11 11160.0 12 14296.0 13 18080.0 14 21977.0 15 28773.0 16 34183.0 17 37840.0 18 32713.0 19 26032.0 20 25279.0 21 25957.0 22 27493.0 23 30944.0 24 36918.0 25 44751.0 26 56012.0 27 68924.0 28 84606.0 29 101446.0 30 119515.0 31 138929.0 32 161259.0 33 186892.0 34 217088.0 35 248529.0 36 259102.0 37 232136.0 38 114745.0 39 7798.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.668994307650266 23.14838135121699 16.616665185141287 20.565959155991454 2 14.568785577185825 23.41896550021235 40.59692303640273 21.415325886199096 3 17.26622227507047 29.19052895382275 32.45973258329051 21.083516187816276 4 11.359237055963836 20.170522407115453 39.969926707159765 28.500313829760948 5 10.999055728477707 40.77520783231054 35.3133273947775 12.91240904443425 6 26.354926772669202 40.918185764335334 18.858439503876863 13.8684479591186 7 24.960326195408662 36.2386311971676 22.51233827550099 16.288704331922755 8 24.836233693829733 36.10569257893102 20.86827720849474 18.18979651874451 9 26.090736470070297 17.467912254751703 22.52484627300762 33.91650500217038 10-11 22.68633200451644 29.26907801395093 28.34842517093018 19.696164810602447 12-13 24.53865444393928 27.44437746996949 28.884134386912685 19.132833699178544 14-15 22.118747801328563 28.278525126057165 28.389142729006416 21.21358434360786 16-17 18.757569392570325 31.667880101027414 29.40496731133324 20.16958319506902 18-19 18.465913415784694 29.616185197513374 33.455444130967074 18.46245725573486 20-21 19.864366402037486 28.50292847606097 33.214884517032885 18.417820604868655 22-23 19.483188548253473 28.5911428026554 32.8689458273322 19.056722821758928 24-25 18.924466473387653 29.045692409862333 33.52572769713513 18.504113419614885 26-27 19.233616211489995 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19.783043611371845 52-53 19.074526114009306 30.18748762303374 31.18085624521647 19.55713001774049 54-55 19.229065568452583 29.903349146781842 31.064040349816974 19.8035449349486 56-57 19.499096710519954 30.404853542399046 30.194850828106247 19.901198918974757 58-59 19.176907617341303 29.781623554292864 30.86925665338565 20.172212174980178 60-61 19.5512120168403 30.165839342288436 30.619746407897875 19.663202232973394 62-63 19.948547087849523 29.702628501127908 30.93163510606891 19.417189304953656 64-65 19.784267833938614 29.57031323936893 30.93832320693648 19.70709571975598 66-67 19.40980726133996 30.01100015720397 30.599103710734806 19.980088870721264 68-69 19.693896596519437 29.897605774463404 30.597455359834015 19.811042269183147 70-71 19.358651427760527 29.74721531873965 31.159778847895375 19.734354405604446 72-73 19.666563174266063 29.74864971318017 30.260235219509855 20.324551893043907 74-75 19.365409152873728 30.11001899207199 30.30090711997897 20.223664735075314 76-77 18.607116813671194 30.117227127869413 31.17889977653553 20.096756281923863 78-79 18.91281478133071 29.86637076900951 30.786058051877696 20.434756397782085 80-81 18.66175494583762 30.550692341147073 30.40894478510947 20.378607927905833 82-83 19.011887135474172 30.40110055935226 29.975039093351075 20.611973211822495 84-85 18.812120927848074 29.858326122707773 30.077627887284653 21.2519250621595 86-87 18.8053060477992 29.91320552016924 30.364325327163694 20.917163104867868 88-89 19.249513101664288 29.358483284168617 30.540966834956123 20.85103677921097 90-91 18.706402974051116 29.588228880143024 30.7984430258131 20.90692511999276 92-93 18.75057862250606 29.896895127062418 30.471258971477667 20.881267278953857 94-95 18.500281740942857 29.464132226572836 30.48383474025379 21.551751292230517 96-97 18.83409797497046 29.282636285975766 30.036864555634242 21.84640118341953 98-99 18.678733657311703 29.13484442310102 30.15620135875039 22.030220560836888 100-101 18.632704216954107 29.053440840901267 30.585483350955673 21.728371591188957 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6413.0 1 4389.5 2 2439.5 3 2568.0 4 2365.5 5 2531.5 6 4512.0 7 8741.0 8 12231.5 9 13061.5 10 11468.5 11 8937.5 12 7647.5 13 7047.5 14 6561.0 15 6086.0 16 5829.0 17 5900.0 18 6060.0 19 6297.0 20 6812.0 21 7526.0 22 8566.5 23 10001.0 24 11657.0 25 14623.0 26 19748.0 27 25089.5 28 28775.0 29 33303.0 30 40482.5 31 48923.0 32 60837.5 33 74924.5 34 88258.0 35 98214.0 36 109120.5 37 117641.0 38 115483.5 39 110274.0 40 104049.5 41 97221.0 42 90810.5 43 87548.0 44 86511.0 45 83223.5 46 77908.5 47 73619.0 48 78879.5 49 74983.0 50 60162.5 51 50649.5 52 42579.5 53 36687.5 54 32219.0 55 28719.5 56 25712.5 57 22999.0 58 20656.0 59 19109.5 60 17945.0 61 16206.0 62 13109.5 63 9010.0 64 5840.5 65 4043.0 66 2901.5 67 2239.0 68 1639.0 69 1101.0 70 865.5 71 754.0 72 600.5 73 482.0 74 388.5 75 307.5 76 227.5 77 142.5 78 87.5 79 56.5 80 37.0 81 25.0 82 20.5 83 12.0 84 6.5 85 4.5 86 2.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0212718247069981 2 0.0212718247069981 3 2.4686837184137065E-4 4 0.09780101997782298 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.008763827200368658 12-13 0.0 14-15 0.0 16-17 6.377432939235407E-4 18-19 8.228945728045688E-5 20-21 0.0 22-23 1.4400655024079953E-4 24-25 0.004402485964504443 26-27 0.0028801310048159902 28-29 8.640393014447972E-4 30-31 1.2343418592068533E-4 32-33 6.171709296034266E-4 34-35 0.0026949797259349624 36-37 0.09261678416915421 38-39 0.10777861667307839 40-41 0.08490214754911138 42-43 0.08846116657649114 44-45 0.08778227855392737 46-47 0.08572504212191594 48-49 0.0800059248409242 50-51 0.07975905646908282 52-53 0.06328059264867134 54-55 0.05303555521725445 56-57 0.029397908613443213 58-59 0.02203300218684233 60-61 0.013248602622153556 62-63 0.0027772691832154196 64-65 0.006706590768357236 66-67 0.1506514239161964 68-69 0.6905525531332739 70-71 0.8405662337555467 72-73 0.34425794453279135 74-75 0.052397811923330914 76-77 0.18745538368488074 78-79 0.013392609172394355 80-81 0.0027978415475355335 82-83 0.0018309404244901653 84-85 1.6457891456091376E-4 86-87 0.001995519339051079 88-89 3.9087492208217015E-4 90-91 0.003703025577620559 92-93 0.004279051778583757 94-95 0.07387536027353016 96-97 0.036022209924519995 98-99 0.014174359016558695 100-101 0.01195254366998636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2430445.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.32505749786696 #Duplication Level Percentage of deduplicated Percentage of total 1 68.24329106197519 22.059683074220178 2 13.927595343241023 9.004206405545803 3 5.808206007039651 5.632517794110389 4 3.2036923206733605 4.142381538849648 5 1.969420616325141 3.1830817330097383 6 1.3267027017002613 2.573144466902183 7 0.9395638635108922 2.126001913763533 8 0.6965729090143706 1.8013407468276783 9 0.525495181828079 1.5287995770700233 >10 2.8555506597111537 16.850148776639355 >50 0.25500556984288175 5.828595551296287 >100 0.22190222911054538 14.296986409032165 >500 0.01917500384557878 4.1071743616917 >1k 0.0071743211667131485 3.8069137141996916 >5k 2.6088440606229633E-4 0.5988768398158966 >10k+ 3.913266090934445E-4 2.4601470970258736 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23183 0.9538582440664158 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20110 0.8274204929549939 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15052 0.6193104554927183 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8656 0.35614877110981735 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5547 0.2282298097673471 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4770 0.19626035561388966 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4754 0.19560203995564596 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4625 0.1902943699610565 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3978 0.16367373053082873 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3450 0.14194931380878809 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2439 0.10035199315351714 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 4.114472864022843E-5 0.0 4.114472864022843E-5 0.0 5 0.0 4.114472864022843E-5 0.0 4.114472864022843E-5 0.0 6 0.0 4.114472864022843E-5 0.0 4.114472864022843E-5 0.0 7 0.0 4.114472864022843E-5 0.0 4.114472864022843E-5 0.0 8 0.0 4.114472864022843E-5 0.0 4.114472864022843E-5 0.0 9 0.0 4.114472864022843E-5 0.0 4.114472864022843E-5 0.0 10-11 0.0 4.114472864022843E-5 0.0 4.114472864022843E-5 0.0 12-13 0.0 4.114472864022843E-5 0.0 8.228945728045687E-5 0.0 14-15 0.0 4.114472864022843E-5 0.0 1.234341859206853E-4 0.0 16-17 0.0 4.114472864022843E-5 0.0 2.674407361614848E-4 0.0 18-19 0.0 4.114472864022843E-5 0.0 2.8801310048159906E-4 0.0 20-21 0.0 4.114472864022843E-5 0.0 3.085854648017133E-4 0.0 22-23 0.0 4.114472864022843E-5 0.0 6.377432939235407E-4 0.0 24-25 0.0 4.114472864022843E-5 0.0 0.001419493138087881 0.0 26-27 0.0 4.114472864022843E-5 0.0 0.0037235979419406734 0.0 28-29 0.0 4.114472864022843E-5 0.0 0.006891742047238263 0.0 30-31 0.0 4.114472864022843E-5 0.0 0.015429273240085663 0.0 32-33 0.0 4.114472864022843E-5 0.0 0.030015079543046642 0.0 34-35 0.0 4.114472864022843E-5 0.0 0.051945219908288404 0.0 36-37 0.0 4.114472864022843E-5 0.0 0.08811143638304919 0.0 38-39 0.0 4.114472864022843E-5 0.0 0.13750568311564343 0.0 40-41 0.0 4.114472864022843E-5 0.0 0.19272190895083 0.0 42-43 0.0 4.114472864022843E-5 0.0 0.24884331881610156 0.0 44-45 0.0 4.114472864022843E-5 0.0 0.3138519900676625 0.0 46-47 0.0 4.114472864022843E-5 0.0 0.39632659862700037 0.0 48-49 0.0 4.114472864022843E-5 0.0 0.48579581105517716 4.114472864022843E-5 50-51 0.0 4.114472864022843E-5 0.0 0.5839465612264421 4.114472864022843E-5 52-53 0.0 4.114472864022843E-5 0.0 0.6830642125207524 4.114472864022843E-5 54-55 0.0 4.114472864022843E-5 0.0 0.7878804087317344 4.114472864022843E-5 56-57 0.0 4.114472864022843E-5 0.0 0.9077967203536801 4.114472864022843E-5 58-59 0.0 4.114472864022843E-5 0.0 1.0316834982894079 4.114472864022843E-5 60-61 0.0 4.114472864022843E-5 0.0 1.168037129003125 4.114472864022843E-5 62-63 0.0 4.114472864022843E-5 0.0 1.2952772023230312 4.114472864022843E-5 64-65 0.0 4.114472864022843E-5 0.0 1.4282775376525698 4.114472864022843E-5 66-67 0.0 4.114472864022843E-5 0.0 1.568087325572066 4.114472864022843E-5 68-69 0.0 4.114472864022843E-5 0.0 1.7244784391335743 4.114472864022843E-5 70-71 0.0 4.114472864022843E-5 0.0 1.9289677404755097 4.114472864022843E-5 72-73 0.0 4.114472864022843E-5 0.0 2.1541322679591595 4.114472864022843E-5 74-75 0.0 4.114472864022843E-5 0.0 2.3936357333739293 4.114472864022843E-5 76-77 0.0 4.114472864022843E-5 0.0 2.662948554688545 4.114472864022843E-5 78-79 0.0 4.114472864022843E-5 0.0 2.9684275924779207 4.114472864022843E-5 80-81 0.0 4.114472864022843E-5 0.0 3.302543361400896 4.114472864022843E-5 82-83 0.0 4.114472864022843E-5 0.0 3.6445383458584746 4.114472864022843E-5 84-85 0.0 4.114472864022843E-5 0.0 3.994803420772739 4.114472864022843E-5 86-87 0.0 4.114472864022843E-5 0.0 4.363172176288704 4.114472864022843E-5 88-89 0.0 4.114472864022843E-5 0.0 4.74960346767773 4.114472864022843E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 500 0.0 34.14096 9 GGTATCA 18525 0.0 29.347544 1 GTATCAA 25350 0.0 24.686638 1 GTACATG 16915 0.0 24.05655 1 ACCGCGC 120 1.7317475E-4 23.709002 8 CGTCACT 350 0.0 23.062447 1 GAGTACT 16130 0.0 22.970285 12-13 GGTACTC 540 0.0 22.850166 3 TACATGG 17595 0.0 22.662931 2 GGATATG 1030 0.0 22.127306 1 ACATGGG 17460 0.0 21.527359 3 AGTACTT 16965 0.0 21.141062 12-13 GTACTTT 17460 0.0 21.0712 14-15 TCGACCG 70 4.915821E-4 20.319492 18-19 CATGGGA 12710 0.0 20.27891 4 ATCAACG 30680 0.0 20.217619 3 GATTGGC 1110 0.0 20.077894 9 TCAACGC 30830 0.0 20.076794 4 TTAGGCA 970 0.0 20.063303 4 GTGGTAC 710 0.0 20.062609 1 >>END_MODULE