##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939469_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1438631 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.386662041899555 31.0 27.0 31.0 10.0 33.0 2 27.388909317260644 31.0 27.0 31.0 16.0 34.0 3 27.883519123388833 31.0 27.0 31.0 16.0 34.0 4 31.58848933465218 35.0 32.0 37.0 19.0 37.0 5 31.860657110822718 35.0 32.0 37.0 19.0 37.0 6 32.03403374458079 35.0 32.0 37.0 19.0 37.0 7 31.51983239621557 35.0 32.0 37.0 17.0 37.0 8 31.499332351381277 35.0 32.0 37.0 17.0 37.0 9 32.40135309193254 37.0 30.0 39.0 17.0 39.0 10-11 32.08716967728347 35.0 30.0 39.0 17.0 39.0 12-13 31.89999659398414 35.0 30.0 39.0 17.0 39.0 14-15 32.41559371374592 36.0 30.0 39.0 17.0 40.5 16-17 32.533129065062546 36.0 30.5 39.0 17.0 40.0 18-19 33.01827779326318 37.0 31.0 39.5 17.5 41.0 20-21 32.93748188381871 37.0 31.0 40.0 18.0 41.0 22-23 32.842529807852046 37.0 31.0 40.0 17.0 41.0 24-25 32.65616617464798 37.0 30.5 40.0 16.5 41.0 26-27 32.443048981983566 36.5 30.0 39.5 16.0 41.0 28-29 32.44845099264509 36.0 30.0 39.0 16.0 41.0 30-31 32.12331688945949 36.0 30.0 39.0 15.5 40.0 32-33 31.52842528765194 35.0 30.0 39.0 11.5 40.0 34-35 31.1062072206146 35.0 29.0 39.0 9.0 40.0 36-37 30.66819636167996 35.0 27.0 38.5 9.0 40.0 38-39 30.07489794116768 35.0 25.5 38.0 9.0 40.0 40-41 29.908221774728894 35.0 25.5 38.0 8.0 40.0 42-43 29.451083356329733 35.0 25.0 38.0 4.5 40.0 44-45 29.215565701003246 34.5 24.5 38.0 2.0 40.0 46-47 28.341331793906846 34.0 23.0 38.0 2.0 40.0 48-49 27.634787516743348 33.0 21.5 38.0 2.0 40.0 50-51 27.069682913825712 33.0 20.5 37.5 2.0 39.5 52-53 27.25715176442048 33.0 21.5 38.0 2.0 39.5 54-55 27.212639655339 33.0 20.5 38.0 2.0 40.0 56-57 26.909588004151168 33.0 19.0 38.0 2.0 40.0 58-59 26.55159905493487 33.0 18.0 38.0 2.0 40.0 60-61 25.946572818186176 32.5 14.0 37.5 2.0 39.5 62-63 25.225546717678128 31.0 10.0 36.5 2.0 39.0 64-65 24.500074723817296 31.0 8.0 36.0 2.0 39.0 66-67 24.173114579068574 31.0 7.5 36.0 2.0 39.0 68-69 23.888444639382858 30.5 4.0 35.0 2.0 38.0 70-71 23.35174898914315 30.0 2.0 35.0 2.0 38.0 72-73 22.667498823534316 29.0 2.0 34.5 2.0 37.0 74-75 21.972603815710908 29.0 2.0 34.0 2.0 36.5 76-77 21.156348987335875 27.5 2.0 34.0 2.0 36.0 78-79 20.381495324374352 26.5 2.0 33.5 2.0 35.5 80-81 19.720645530368802 26.0 2.0 33.0 2.0 35.0 82-83 18.96393620045724 25.5 2.0 32.5 2.0 35.0 84-85 18.221991254185404 24.0 2.0 32.0 2.0 34.5 86-87 17.44407495737267 21.0 2.0 32.0 2.0 34.0 88-89 16.389471657429876 16.5 2.0 31.0 2.0 34.0 90-91 15.508437535406925 7.5 2.0 31.0 2.0 34.0 92-93 14.749971674459955 2.0 2.0 30.0 2.0 34.0 94-95 13.947297812990266 2.0 2.0 29.5 2.0 34.0 96-97 12.877054296758516 2.0 2.0 29.0 2.0 34.0 98-99 11.853407162781838 2.0 2.0 28.5 2.0 33.0 100-101 10.515576614156096 2.0 2.0 25.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.2508247424977412 1101 2 3.289173822795238 1101 3 2.6509125358365253 1101 4 2.7666330582661516 1101 5 2.0625424610517875 1101 6 1.9349643644956132 1101 7 2.4941731800432123 1101 8 2.6202582518623068 1101 9 2.762447484203797 1101 10-11 2.630933599726493 1101 12-13 2.717784137098608 1101 14-15 2.437310581726855 1101 16-17 2.1795367240313084 1101 18-19 2.365922826913014 1101 20-21 2.39672078547472 1101 22-23 2.589077597115626 1101 24-25 2.7126302386377503 1101 26-27 2.6082523612411634 1101 28-29 2.3274584354343943 1101 30-31 2.467982863385263 1101 32-33 2.7038111048986018 1101 34-35 2.8906954292188622 1101 36-37 2.9514565152557246 1101 38-39 3.0881221888694945 1101 40-41 2.659628633608232 1101 42-43 2.569021202326681 1101 44-45 2.399872472959604 1101 46-47 2.9060192964326887 1101 48-49 2.3752365817277514 1101 50-51 2.279931429389226 1101 52-53 2.177705369035703 1101 54-55 2.3060804137901805 1101 56-57 2.6399293139059807 1101 58-59 2.1876456139661933 1101 60-61 1.9847367619194785 1101 62-63 2.1205206303372925 1101 64-65 2.081116678746035 1101 66-67 2.185758586973723 1101 68-69 2.0327841217779152 1101 70-71 1.7655157784124498 1101 72-73 1.70244532164536 1101 74-75 1.4915171572807466 1101 76-77 1.3882535986213398 1101 78-79 1.4379723907081114 1101 80-81 1.2323834916744154 1101 82-83 1.485515174917868 1101 84-85 1.242382850690607 1101 86-87 0.5597081736625924 1101 88-89 0.6168007389060932 1101 90-91 0.6021492035853804 1101 92-93 0.4740404574443797 1101 94-95 0.37618916801943136 1101 96-97 0.267649319568493 1101 98-99 0.015851702270348156 1101 100-101 -0.05404223870973368 1102 1 3.4741825677995983 1102 2 3.45107853657273 1102 3 2.945691232203494 1102 4 2.704855689249335 1102 5 2.3473719268775746 1102 6 2.013872635841018 1102 7 2.6129129048707256 1102 8 2.6428863714720485 1102 9 2.8093940735203162 1102 10-11 3.096188777739652 1102 12-13 3.105196461241558 1102 14-15 2.9884198792419383 1102 16-17 2.7958883134825783 1102 18-19 2.7731969752528087 1102 20-21 2.7964435242629904 1102 22-23 2.833259912364653 1102 24-25 3.1274899067328406 1102 26-27 2.9599395108746265 1102 28-29 2.7775185704602734 1102 30-31 3.0091315602912623 1102 32-33 3.2359437590598823 1102 34-35 3.276917265139513 1102 36-37 3.104458465854041 1102 38-39 2.861225324650011 1102 40-41 2.7731444438982287 1102 42-43 3.1621163706373743 1102 44-45 2.6502847432000074 1102 46-47 2.817225382703711 1102 48-49 2.8388464688019184 1102 50-51 2.726555698335968 1102 52-53 2.546031981395558 1102 54-55 2.4457486436306297 1102 56-57 2.7580655829066245 1102 58-59 2.778893777910781 1102 60-61 2.7428057201180565 1102 62-63 2.730586256684859 1102 64-65 2.55157640006988 1102 66-67 2.506483357214126 1102 68-69 2.58109259256274 1102 70-71 2.279353572181318 1102 72-73 2.2856624276177655 1102 74-75 2.158982676497004 1102 76-77 1.9568765907515662 1102 78-79 2.002768302200135 1102 80-81 1.6684260174128802 1102 82-83 1.9058577424308076 1102 84-85 1.9817455204836207 1102 86-87 1.565721255136289 1102 88-89 1.3699873057183005 1102 90-91 1.4796805175946517 1102 92-93 1.540930145887673 1102 94-95 1.3660243273014494 1102 96-97 1.0510352078824194 1102 98-99 1.0122208605821204 1102 100-101 0.7026197788624966 1103 1 3.5686792280419617 1103 2 3.6401782870809534 1103 3 2.935178586006593 1103 4 2.9939130327559482 1103 5 2.2868462450653873 1103 6 2.3389025022507184 1103 7 2.8948295363357346 1103 8 2.5884635027126457 1103 9 3.0569973353942785 1103 10-11 3.0442055491312594 1103 12-13 2.9536937353128927 1103 14-15 2.9656574673561025 1103 16-17 2.63018377972859 1103 18-19 2.514307180654505 1103 20-21 2.827976100100571 1103 22-23 2.89661506522787 1103 24-25 2.9454706404234656 1103 26-27 3.0165219190642887 1103 28-29 2.670211943087452 1103 30-31 2.7120969152560157 1103 32-33 2.8694148463952054 1103 34-35 2.932415242144028 1103 36-37 2.852305526735318 1103 38-39 3.057124633597393 1103 40-41 2.841315761584422 1103 42-43 2.836725549690623 1103 44-45 2.5342727174323905 1103 46-47 2.582498091075543 1103 48-49 2.912399102986253 1103 50-51 2.6869719799844596 1103 52-53 2.4872623949710793 1103 54-55 2.464031094062289 1103 56-57 2.5566026238549604 1103 58-59 2.6967801271464644 1103 60-61 2.742529332072543 1103 62-63 2.4278654432624656 1103 64-65 2.1808987790861742 1103 66-67 2.4156626579771228 1103 68-69 2.469832378580641 1103 70-71 2.3228871644668736 1103 72-73 2.209646639672574 1103 74-75 2.3288165301548993 1103 76-77 2.1333375697097736 1103 78-79 2.1069509196196776 1103 80-81 2.1322492973716898 1103 82-83 2.0692006022137868 1103 84-85 2.1090123149763222 1103 86-87 1.7726851613326637 1103 88-89 1.811867437460517 1103 90-91 1.6527377983983058 1103 92-93 1.4098929234307729 1103 94-95 1.157000711386777 1103 96-97 1.1013015304528455 1103 98-99 0.6194470466581041 1103 100-101 0.49950261673244256 1104 1 3.6798879788944845 1104 2 3.555632290018888 1104 3 2.9648300995683137 1104 4 2.879733556338131 1104 5 2.3496200371014844 1104 6 2.2498643484287726 1104 7 2.8586611165256457 1104 8 2.8252493481562233 1104 9 3.222061143693942 1104 10-11 3.188497231099671 1104 12-13 3.442320709238711 1104 14-15 3.1449133760177688 1104 16-17 2.939986026462755 1104 18-19 2.8641822527913305 1104 20-21 3.2160927059974256 1104 22-23 3.0579154626361955 1104 24-25 3.07236423205034 1104 26-27 3.0538928925179363 1104 28-29 2.9856113515654954 1104 30-31 3.1349055952833034 1104 32-33 3.4273677672807814 1104 34-35 3.726619540187155 1104 36-37 3.6131416253135633 1104 38-39 3.3788901167829515 1104 40-41 3.4578232900456776 1104 42-43 3.4471383362705588 1104 44-45 3.2544834881159908 1104 46-47 3.4210894884314023 1104 48-49 3.454273709512666 1104 50-51 3.156513845573887 1104 52-53 2.915729716989862 1104 54-55 2.928094659719914 1104 56-57 3.428346629940684 1104 58-59 3.2635706286941257 1104 60-61 3.2276347174143396 1104 62-63 2.976769291434124 1104 64-65 2.880063631402418 1104 66-67 2.8038054954463014 1104 68-69 2.9581503117950554 1104 70-71 2.662550777341327 1104 72-73 2.8697379255442463 1104 74-75 2.7642057083197358 1104 76-77 2.6406201099752344 1104 78-79 2.5664100245984294 1104 80-81 2.6023985878076594 1104 82-83 2.6289594029063004 1104 84-85 2.2003580274258248 1104 86-87 1.9151660450743364 1104 88-89 1.7320660760419138 1104 90-91 1.5146343082876044 1104 92-93 1.2515616121144735 1104 94-95 1.4392163611428241 1104 96-97 1.1280151079146794 1104 98-99 0.5240608523346157 1104 100-101 0.5437077345122017 1105 1 3.4255488189785233 1105 2 3.1885146584915205 1105 3 1.9643417162870591 1105 4 2.7082903715931863 1105 5 2.397807997441202 1105 6 2.525487462321699 1105 7 3.0733258167004323 1105 8 2.8798761925522847 1105 9 3.1827090331168435 1105 10-11 3.1523582856512 1105 12-13 3.667684080978571 1105 14-15 3.316974329166989 1105 16-17 3.136301526921976 1105 18-19 2.7746145408386482 1105 20-21 3.049054689854046 1105 22-23 3.0732489158380574 1105 24-25 2.9825436217899224 1105 26-27 3.232267799174366 1105 28-29 3.0876941888212883 1105 30-31 3.199105116457101 1105 32-33 3.5245125069149914 1105 34-35 3.6407298888911974 1105 36-37 3.6505171199737454 1105 38-39 3.7429482659803988 1105 40-41 3.5138672963760307 1105 42-43 3.3584781425046835 1105 44-45 3.1527713928950156 1105 46-47 3.289853894928278 1105 48-49 3.4580837875406054 1105 50-51 3.064306798599997 1105 52-53 2.512902601431737 1105 54-55 2.8607925278418485 1105 56-57 3.1373503766422743 1105 58-59 3.0738493149350035 1105 60-61 3.1846551663381923 1105 62-63 3.3497414479176335 1105 64-65 3.1702072387649416 1105 66-67 3.0415787025613064 1105 68-69 3.01753079395813 1105 70-71 3.014479526837153 1105 72-73 3.025182305734841 1105 74-75 2.980709742543823 1105 76-77 2.743776499535013 1105 78-79 2.958665423946929 1105 80-81 2.8744256456993007 1105 82-83 2.7237527349761557 1105 84-85 2.8517898489577753 1105 86-87 2.4789020880844603 1105 88-89 2.7062303574867492 1105 90-91 2.879391420622241 1105 92-93 2.8915883071608253 1105 94-95 2.3928270362456434 1105 96-97 2.22715428176131 1105 98-99 1.9697409685746958 1105 100-101 1.693139452374071 1106 1 2.92928034581249 1106 2 2.346780119263638 1106 3 2.5637237773173176 1106 4 1.3236584997159682 1106 5 1.5942039000232846 1106 6 1.8064259893407169 1106 7 2.424197091908148 1106 8 2.5240178359701098 1106 9 2.718779287952252 1106 10-11 2.645877305194272 1106 12-13 2.977033043154993 1106 14-15 2.7202825748340373 1106 16-17 1.3075172363454328 1106 18-19 2.4197874489700055 1106 20-21 1.595900811806814 1106 22-23 2.3603574627444956 1106 24-25 2.563008036422758 1106 26-27 2.684537987015638 1106 28-29 2.038911444263391 1106 30-31 1.1154450109998884 1106 32-33 1.3387437257164443 1106 34-35 2.085659416207701 1106 36-37 2.490130568462046 1106 38-39 0.9908459148608237 1106 40-41 0.6104120618145465 1106 42-43 1.8499481017225925 1106 44-45 2.2934731448152412 1106 46-47 2.3761258349240144 1106 48-49 2.3677266103832615 1106 50-51 2.2570396910837722 1106 52-53 2.077567173945866 1106 54-55 2.262729267569611 1106 56-57 2.3996453242064746 1106 58-59 2.457437038609932 1106 60-61 2.4515905123067157 1106 62-63 2.2034570460745933 1106 64-65 2.337423796156198 1106 66-67 2.6983086857190735 1106 68-69 2.753504188382628 1106 70-71 2.7387916070989426 1106 72-73 2.6580825196007503 1106 74-75 2.1547967131202768 1106 76-77 2.129710189389616 1106 78-79 2.421529353223601 1106 80-81 2.232909891535005 1106 82-83 2.063660746866443 1106 84-85 2.0842208695877567 1106 86-87 1.8706192931638341 1106 88-89 2.0866434724098113 1106 90-91 1.9537443766801736 1106 92-93 1.971629909117242 1106 94-95 2.0594755489054286 1106 96-97 1.7014124856155792 1106 98-99 1.362082437245565 1106 100-101 1.3420766816744045 1107 1 2.7600057586542057 1107 2 3.0668364503462584 1107 3 -0.4838265160342239 1107 4 1.6801375225518669 1107 5 0.4552135920041671 1107 6 1.405866787965003 1107 7 2.3323295363357346 1107 8 1.2861675843452964 1107 9 -1.880502664605725 1107 10-11 -0.9800291447462932 1107 12-13 1.9116018985782013 1107 14-15 1.5903385898050786 1107 16-17 -0.4017039753734579 1107 18-19 0.5784015684096104 1107 20-21 0.7750424266311811 1107 22-23 0.7151737386972599 1107 24-25 0.21555992613775032 1107 26-27 0.8360372251867361 1107 28-29 0.7732093920670451 1107 30-31 1.5717907928070396 1107 32-33 -0.05564892911500152 1107 34-35 1.1333080992868823 1107 36-37 2.0031346083679686 1107 38-39 -0.22444424395363072 1107 40-41 0.5626167819925811 1107 42-43 0.18270769254776908 1107 44-45 0.46635179906504476 1107 46-47 0.6293730910755464 1107 48-49 0.15410828665972431 1107 50-51 1.1518954493722156 1107 52-53 1.1789078031343472 1107 54-55 0.642602522633716 1107 56-57 0.7192301748753707 1107 58-59 0.7385530863301355 1107 60-61 1.1586645361541699 1107 62-63 1.3012710555073603 1107 64-65 1.330771228065764 1107 66-67 0.8474228620587532 1107 68-69 0.45643952143777966 1107 70-71 0.028244307324012397 1107 72-73 0.5425548029378788 1107 74-75 0.6881915301548993 1107 76-77 0.6454549166485499 1107 78-79 1.1260835726809013 1107 80-81 1.3181549096165917 1107 82-83 1.486611316499502 1107 84-85 1.2254026210987696 1107 86-87 1.244623936842867 1107 88-89 1.3074031517462323 1107 90-91 1.2372403494187125 1107 92-93 1.4443316989409762 1107 94-95 1.1263884664888177 1107 96-97 1.2186484692283575 1107 98-99 1.163452148698921 1107 100-101 1.2153827187732578 1108 1 2.675988421311743 1108 2 3.046601820444142 1108 3 2.4274607496557508 1108 4 2.6625602722159982 1108 5 2.1758612632983905 1108 6 1.885280293179001 1108 7 2.0660681806962984 1108 8 2.1925467347515415 1108 9 2.5169419166750657 1108 10-11 -0.7044089828884488 1108 12-13 1.7458987285954741 1108 14-15 1.455265657810962 1108 16-17 1.5668654939503242 1108 18-19 -0.08203302470663942 1108 20-21 -0.23090868814074383 1108 22-23 0.7503218418256594 1108 24-25 -0.3279647603826277 1108 26-27 0.6793991741983163 1108 28-29 0.08402803770712808 1108 30-31 0.08772187527137021 1108 32-33 1.138290598106547 1108 34-35 -0.8564620289838594 1108 36-37 0.45116936302218136 1108 38-39 1.1061052250502748 1108 40-41 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0.7240207557197458 2312 62-63 -4.373565886642151 2312 64-65 -2.155426132916162 2312 66-67 1.0004604343262677 2312 68-69 -0.4812106559253877 2312 70-71 -1.0576277928565396 2312 72-73 -1.701224947568221 2312 74-75 -1.2076343940485614 2312 76-77 -1.5523637215305826 2312 78-79 -0.2719702198816982 2312 80-81 0.4172121263370592 2312 82-83 -1.011069910817163 2312 84-85 -1.6059017505158302 2312 86-87 -0.7062458380107728 2312 88-89 -2.823799999755657 2312 90-91 -2.5407142975875434 2312 92-93 -1.8172980702589285 2312 94-95 -1.6814882364711838 2312 96-97 -1.2862632139435721 2312 98-99 -0.4167454128994468 2312 100-101 -0.2142194337499479 2313 1 -2.124419894780665 2313 2 -0.9759982292971756 2313 3 -2.4089078061788776 2313 4 -2.0853687671479726 2313 5 -1.5827698119066582 2313 6 -2.9826433894276647 2313 7 0.5377339409048822 2313 8 -2.6938145785285705 2313 9 -3.5579881858412463 2313 10-11 -4.971522592604948 2313 12-13 -3.999604464961113 2313 14-15 -3.7262748472000595 2313 16-17 -1.9240507028613116 2313 18-19 -4.150051817085828 2313 20-21 -6.190886605182069 2313 22-23 -4.962254512463712 2313 24-25 -1.5014600171926773 2313 26-27 -5.127889022077646 2313 28-29 -5.360332745363337 2313 30-31 -4.950768612384856 2313 32-33 -4.2411222357000575 2313 34-35 0.6286644970770361 2313 36-37 0.6734307891768232 2313 38-39 -2.3620597849389853 2313 40-41 -5.966023259464283 2313 42-43 -2.8589104150265605 2313 44-45 -1.5553966507508044 2313 46-47 -3.2550419979766403 2313 48-49 -3.0926103604765522 2313 50-51 -2.4889138713046144 2313 52-53 -4.406070979137066 2313 54-55 -3.631551270313281 2313 56-57 -3.1693072475042214 2313 58-59 -1.3090063744637703 2313 60-61 0.5360773855572276 2313 62-63 0.5980003782303491 2313 64-65 0.9597868055260399 2313 66-67 0.3005840033679341 2313 68-69 -2.603085492796332 2313 70-71 -0.9194856292452052 2313 72-73 -2.267107270261061 2313 74-75 -2.3548872423305554 2313 76-77 -1.329528069135126 2313 78-79 -1.2170731538283626 2313 80-81 -1.2414054118964977 2313 82-83 -1.0853652127782958 2313 84-85 -1.3690184952117477 2313 86-87 -1.2291088376531576 2313 88-89 -1.8041567293989047 2313 90-91 -3.7092777412526043 2313 92-93 -4.015022950721852 2313 94-95 -3.271711946300533 2313 96-97 -2.7984424778741115 2313 98-99 -2.1752497444384584 2313 100-101 -2.2618885679018295 2314 1 -2.045427728609315 2314 2 -0.3535326096865141 2314 3 -2.376483487581968 2314 4 -1.218454761076913 2314 5 -2.2798294215516215 2314 6 -2.3311453526576713 2314 7 -1.053409887170897 2314 8 0.45670422966035673 2314 9 -1.4496828001635969 2314 10-11 -2.251382302797836 2314 12-13 -1.2264092365327777 2314 14-15 -3.582451146527408 2314 16-17 -1.5957374738093257 2314 18-19 -1.161373401797455 2314 20-21 -1.5145379584425562 2314 22-23 -1.9722890201288976 2314 24-25 -2.164572373132323 2314 26-27 -1.672465400316021 2314 28-29 -1.8312008939451658 2314 30-31 -1.7118519526122995 2314 32-33 -8.445177642060035 2314 34-35 -9.272492679053649 2314 36-37 -5.508219294507047 2314 38-39 -3.3243483479309894 2314 40-41 -2.533162861981797 2314 42-43 -1.6601811053071422 2314 44-45 -1.7575584310854104 2314 46-47 -3.184658452198697 2314 48-49 -2.405875606532593 2314 50-51 -1.9433069564076142 2314 52-53 -1.4432339367732965 2314 54-55 -0.5276711068910842 2314 56-57 -0.7287764912947488 2314 58-59 -3.230296999324562 2314 60-61 -3.872820548755705 2314 62-63 -3.464884631219885 2314 64-65 -3.726412594368316 2314 66-67 -3.5035495980931906 2314 68-69 -4.845532864231814 2314 70-71 -6.589230647986575 2314 72-73 -6.694690095021304 2314 74-75 -6.203441309112195 2314 76-77 -7.1550953216949615 2314 78-79 -6.41118553204128 2314 80-81 -5.614201153246512 2314 82-83 -5.016658924078483 2314 84-85 -4.7032432898203425 2314 86-87 -4.6866730801391085 2314 88-89 -4.774406396890743 2314 90-91 -5.204593592187127 2314 92-93 -5.453974293163894 2314 94-95 -4.759802506250642 2314 96-97 -5.281202566076277 2314 98-99 -5.018834086963228 2314 100-101 -4.518163665108761 2315 1 -25.01805546583559 2315 2 -13.021275590877417 2315 3 -8.057338929981839 2315 4 -5.715285916284746 2315 5 -2.0798680304651818 2315 6 -1.9955574044664637 2315 7 -7.706749193958782 2315 8 -1.6277418417005691 2315 9 -5.034899228620553 2315 10-11 -3.3290390331327835 2315 12-13 -3.1304861192636686 2315 14-15 -3.1625892280286365 2315 16-17 -5.4592540537880545 2315 18-19 -6.411709408615934 2315 20-21 -5.0389781291959395 2315 22-23 -6.798859419476191 2315 24-25 -24.352460283443087 2315 26-27 -10.202750632770815 2315 28-29 -6.640749679585568 2315 30-31 -4.719131189758446 2315 32-33 -5.247941639612375 2315 34-35 -6.3942191166893245 2315 36-37 -5.09793493621633 2315 38-39 -3.7761609635756095 2315 40-41 -3.8204678141213115 2315 42-43 -3.408525657079508 2315 44-45 -8.43189952990966 2315 46-47 -4.745671462674096 2315 48-49 -4.040794637084627 2315 50-51 -6.05948154154391 2315 52-53 -9.337383593663898 2315 54-55 -5.051111452477286 2315 56-57 -11.951592720150906 2315 58-59 -7.487923170340196 2315 60-61 -4.825886132915848 2315 62-63 -5.189281640121788 2315 64-65 -5.105970085226023 2315 66-67 -8.348655007711908 2315 68-69 -5.7499238996791675 2315 70-71 -5.867117392745232 2315 72-73 -5.868717041129091 2315 74-75 -5.5176149690915075 2315 76-77 -6.196444193294829 2315 78-79 -5.568631588832805 2315 80-81 -4.9908711708754865 2315 82-83 -4.386110713929437 2315 84-85 -4.324304087932148 2315 86-87 -4.373743282799481 2315 88-89 -5.457322498993108 2315 90-91 -5.2205316066691125 2315 92-93 -5.135412511617497 2315 94-95 -5.442700396601326 2315 96-97 -5.598836458874622 2315 98-99 -5.332502370833444 2315 100-101 -4.559523463759634 2316 1 -1.570833243613368 2316 2 -0.4775796494269855 2316 3 -4.337412116941255 2316 4 -0.4480370806227363 2316 5 -5.380599900059327 2316 6 -1.0380391077266076 2316 7 -4.756998694956785 2316 8 -4.353656678693252 2316 9 -3.4601653630184224 2316 10-11 -2.2586714349957298 2316 12-13 -4.421603883734726 2316 14-15 -3.862580911328706 2316 16-17 -2.0116742134686945 2316 18-19 -6.02782717308699 2316 20-21 -2.860310464525284 2316 22-23 -6.086725354273263 2316 24-25 -2.1833984071955825 2316 26-27 -2.83063652764773 2316 28-29 -5.552966911787827 2316 30-31 -9.098206372725844 2316 32-33 -6.15903611732362 2316 34-35 -3.0619087374478084 2316 36-37 -5.127775255577607 2316 38-39 -4.1345774639082755 2316 40-41 -3.0755493177806628 2316 42-43 -2.521799110173319 2316 44-45 0.527498341015157 2316 46-47 -1.1673530994006427 2316 48-49 -1.8590082099389171 2316 50-51 0.6155434085558902 2316 52-53 2.184888528757927 2316 54-55 2.411288747123514 2316 56-57 0.48296316807264716 2316 58-59 -2.605388240200476 2316 60-61 -5.860857856136079 2316 62-63 -1.4911113141071475 2316 64-65 -3.2302548490317413 2316 66-67 -2.373247489415167 2316 68-69 1.333218149555698 2316 70-71 1.8937275839680048 2316 72-73 2.112254349423143 2316 74-75 2.2080753170029723 2316 76-77 1.1862570708435634 2316 78-79 1.0984758629303322 2316 80-81 2.0751135263966347 2316 82-83 1.3010316679734224 2316 84-85 0.8311849340239412 2316 86-87 0.08238612505148524 2316 88-89 -1.301667143038351 2316 90-91 -0.7815096505812882 2316 92-93 -1.3834248203333992 2316 94-95 -2.8522396514250126 2316 96-97 -4.341725680431507 2316 98-99 -2.5385319782852083 2316 100-101 -1.8227054331541783 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18448.0 3 6030.0 4 5629.0 5 6193.0 6 7128.0 7 9560.0 8 12518.0 9 15353.0 10 18083.0 11 21206.0 12 25247.0 13 28592.0 14 31515.0 15 35233.0 16 37717.0 17 38428.0 18 33355.0 19 31216.0 20 32997.0 21 35533.0 22 38287.0 23 41907.0 24 44690.0 25 48470.0 26 51899.0 27 55728.0 28 59510.0 29 63839.0 30 68587.0 31 72952.0 32 77530.0 33 81406.0 34 84462.0 35 80866.0 36 68821.0 37 42254.0 38 7392.0 39 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.16699562319734 19.11054956390449 14.560648539000798 17.16180627389737 2 14.120366030416005 23.37063824570765 41.53500580485589 20.973989919020458 3 21.72089964007089 31.526684084555935 30.53002758847496 16.222388686898213 4 10.99316063456062 17.691472056567573 40.281167674629785 31.03419963424202 5 10.722086986795594 42.71244830403545 32.49791531548226 14.067549393686704 6 23.693748364575736 38.93875579705707 19.882096349454674 17.48539948891252 7 24.73326901447033 39.339148456948955 19.92025499149533 16.00732753708539 8 23.39216451212735 32.466910093123246 20.276268446430276 23.864656948319123 9 28.179102940060734 20.09722476475129 21.909469878629135 29.81420241655884 10-11 22.89217965049078 27.54491753823942 26.559350979667904 23.003551831601893 12-13 30.602571786056 25.69655167579654 26.174109004755948 17.526767533391514 14-15 26.556306056527685 26.198901688259845 29.84228517691971 17.402507078292757 16-17 17.921887011898228 30.372308160251794 31.77313149023144 19.932673337618535 18-19 16.991843658874625 30.96461594471759 35.18503693916888 16.858503457238903 20-21 19.692857714761143 28.38665882246459 32.88692164964962 19.03356181312465 22-23 18.615041942465915 27.792696870836785 31.754310510617618 21.837950676079682 24-25 19.020713706258515 28.20483091923635 34.35099896463185 18.423456409873282 26-27 21.440481170491527 25.698058411026963 35.29259327502378 17.56886714345773 28-29 17.96306727432778 26.355402328159993 37.71560665612561 17.965923741386618 30-31 17.337145702986916 31.07592060194577 35.243256232684566 16.34367746238274 32-33 17.31932663047719 30.13718050895634 33.28865381828721 19.25483904227927 34-35 18.60489372202175 27.298022841102785 33.88792249565123 20.209160941224237 36-37 20.57710021674193 27.307357062374905 35.265225693555 16.850317027328163 38-39 22.12415255330019 25.312650069593957 35.78488532156451 16.778312055541345 40-41 18.240405182833666 27.45064220963459 37.940106891501884 16.36884571602986 42-43 16.927511380541922 30.497832543056614 34.94070138683813 17.633954689563332 44-45 17.384862397457805 31.37387396796353 32.30787654970834 18.933387084870333 46-47 17.79129908443859 29.275014252862675 30.06269169969697 22.870994963001763 48-49 19.676559951431187 29.080787620547742 30.881799564111674 20.360852863909397 50-51 22.241858433253313 30.018808261979334 30.816844315734944 16.922488989032402 52-53 18.312326674594757 30.350217058547447 33.40227731158903 17.93517895526876 54-55 16.5585630926382 30.025282643503576 34.028890665636865 19.387263598221363 56-57 18.880412122681165 32.842628173650425 29.564132622037224 18.71282708163119 58-59 17.805884663492744 30.35846609855502 30.715636813411816 21.12001242454042 60-61 18.31926394679805 28.569657825787974 31.89296936883612 21.218108858577857 62-63 23.130045539315912 27.701105288388394 30.484500583645463 18.68434858865023 64-65 21.4362699091261 28.45260938705374 32.443590229176 17.66753047464415 66-67 17.930138834486662 29.511180745963355 33.37400008704356 19.18468033250642 68-69 17.331255286084918 32.70971023984941 31.736670186951134 18.22236428711454 70-71 17.971135310111208 31.76794310356355 29.294951081990593 20.96597050433465 72-73 17.79768294888292 30.528920280939577 28.330199122560355 23.343197647617146 74-75 18.83028420132666 30.680290662901882 30.120101699272368 20.369323436499087 76-77 20.842777193178616 28.41552495669496 31.102997548166734 19.63870030195969 78-79 18.271355026698462 28.47065223940743 32.987500779307446 20.270491954586664 80-81 17.35757675401548 32.769964891649686 30.865740081168248 19.00671827316658 82-83 17.63999074694712 31.63687062866412 29.05416798471574 21.66897063967302 84-85 18.584352193197077 29.030409941262825 29.30607241059254 23.07916545494756 86-87 19.987303457805414 29.211588606960188 30.705922174305485 20.095185760928917 88-89 21.488425834836264 26.743594449248175 31.555144481616765 20.212835234298794 90-91 18.211894926753832 28.62082657063048 33.13499167578035 20.032286826835332 92-93 17.08707792583182 31.110572479500075 31.118512652386894 20.68383694228121 94-95 17.046778839288514 31.656746204508963 29.320938850712423 21.975536105490097 96-97 17.43184274205067 29.62476145220997 27.714219224983573 25.229176580755784 98-99 18.815630389714467 29.09712374348879 28.561105207226355 23.52614065957039 100-101 20.637734976871407 29.392855636359705 29.241697916735664 20.727711470033224 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7804.0 1 5427.5 2 3036.5 3 2998.0 4 2616.5 5 2558.5 6 4092.0 7 7145.0 8 9965.5 9 10781.5 10 9336.5 11 7590.0 12 6880.5 13 6544.5 14 6343.0 15 6116.5 16 5879.5 17 5867.0 18 6121.0 19 6539.5 20 7168.0 21 7896.5 22 8683.0 23 9635.5 24 10655.5 25 12100.5 26 14236.5 27 16541.0 28 18366.0 29 20181.0 30 22627.5 31 25492.0 32 28990.5 33 33260.5 34 37962.0 35 41268.5 36 44435.0 37 47604.5 38 47471.0 39 45346.5 40 42882.5 41 40764.5 42 39033.5 43 38349.5 44 40721.5 45 43996.5 46 50116.5 47 67110.5 48 107233.0 49 105376.0 50 61111.0 51 38424.5 52 25653.5 53 18428.0 54 14189.0 55 11589.0 56 9757.0 57 8516.0 58 7896.5 59 7507.0 60 7058.0 61 6687.5 62 5762.0 63 4321.0 64 3117.0 65 2384.5 66 1920.5 67 1614.0 68 1378.0 69 1161.5 70 997.5 71 862.0 72 711.5 73 581.5 74 482.5 75 373.0 76 269.5 77 204.5 78 136.0 79 86.5 80 59.0 81 37.0 82 30.0 83 24.0 84 17.5 85 10.5 86 7.0 87 7.5 88 4.5 89 2.0 90 1.0 91 0.5 92 1.0 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.5035391285187094 2 3.6048854779300603 3 1.0824874481364575 4 0.948471150698129 5 0.9700194142903913 6 0.11782034448027327 7 1.798793436259889 8 0.9330398135449605 9 0.13624063432527173 10-11 1.0132549625303502 12-13 0.8338482904928366 14-15 0.656005605328955 16-17 0.1323828000369796 18-19 0.14458189765130877 20-21 0.16168148746968472 22-23 0.6044635490268179 24-25 1.377142575128716 26-27 0.2176722175457084 28-29 0.22879390198042443 30-31 0.20529934361208677 32-33 0.22403243083181165 34-35 0.6996929719990741 36-37 0.27633910293883557 38-39 0.26970084754186446 40-41 0.25194090771017724 42-43 0.19789647241022887 44-45 0.16633869282672206 46-47 0.20536885413980377 48-49 0.6191302703750997 50-51 0.2333815968097448 52-53 0.25367867090310164 54-55 0.28151763725375023 56-57 0.20449997254334157 58-59 0.19205758808200296 60-61 0.18027555363397563 62-63 0.1667905112568824 64-65 0.163697292773477 66-67 0.17853779044105125 68-69 0.1854193326850318 70-71 0.19177954597113506 72-73 0.2544432867079884 74-75 0.22399767556795314 76-77 0.22066117023753834 78-79 0.21280648060552013 80-81 0.22365012292936826 82-83 0.23835159954150856 84-85 0.20842731735935066 86-87 0.19511605130154988 88-89 0.19640199606431394 90-91 0.2127717253416616 92-93 0.20116346721292672 94-95 0.211729067425907 96-97 0.23522362579424466 98-99 0.2506549629474132 100-101 0.3035872298038899 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1438631.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.9502382841492 #Duplication Level Percentage of deduplicated Percentage of total 1 86.24577848659996 46.52980300354016 2 7.5586864961011715 8.15585875159678 3 2.4966058772674944 4.040774459405661 4 1.1345349090721843 2.4483371474451956 5 0.6094812709655828 1.6440829899159646 6 0.37655741685393024 1.2189217420159941 7 0.25217971267521205 0.9523608912479178 8 0.18679480280183164 0.8062099297119583 9 0.13729417635950864 0.6666348176659345 >10 0.8607997368439556 8.942475386532458 >50 0.07926776813985162 2.97608669402729 >100 0.05313930941182768 5.322050053749988 >500 0.002537153402152455 0.9673615941023741 >1k 0.0046514479039461674 5.3333022593173425 >5k 0.0011276237342899799 4.588294670494061 >10k+ 5.638118671449899E-4 5.407445609230856 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 25323 1.7602150933769674 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 19699 1.3692878854966979 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14597 1.0146451730846895 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11490 0.7986759634680471 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8125 0.5647730377004249 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8074 0.5612280007868592 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7997 0.555875690152652 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7810 0.5428772214695777 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7794 0.541765053026106 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7681 0.5339103633940878 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6472 0.44987213538426457 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6384 0.44375520894517084 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4996 0.347274596474009 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4956 0.34449417536532995 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4049 0.2814481267260333 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3715 0.2582316104685635 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3603 0.25044643136426226 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3573 0.248361115532753 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3287 0.22848110460569807 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2961 0.20582067256996409 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2854 0.1983830461042477 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2809 0.19525507235698383 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2580 0.17933716150979648 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2191 0.15229756622789303 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1836 0.12762132888836678 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1811 0.1258835656954424 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1763 0.12254706036502758 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1699 0.11809838659114116 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1654 0.11497041284387727 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1568 0.1089925074602174 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.951052771697537E-5 0.0 12-13 0.0 0.0 0.0 6.951052771697537E-5 0.0 14-15 0.0 0.0 0.0 6.951052771697537E-5 0.0 16-17 0.0 0.0 0.0 6.951052771697537E-5 0.0 18-19 0.0 0.0 0.0 1.7377631929243843E-4 0.0 20-21 0.0 0.0 0.0 3.8230790244336453E-4 0.0 22-23 0.0 0.0 0.0 9.383921241791675E-4 0.0 24-25 0.0 0.0 0.0 0.002085315831509261 0.0 26-27 0.0 0.0 0.0 0.003336505330414818 0.0 28-29 0.0 0.0 0.0 0.006777276452405098 0.0 30-31 0.0 0.0 0.0 0.017064834554517454 0.0 32-33 0.0 0.0 0.0 0.03447722174761979 0.0 34-35 0.0 0.0 0.0 0.06721668030231519 0.0 36-37 0.0 0.0 0.0 0.11709048393924502 0.0 38-39 0.0 0.0 0.0 0.18055359574484353 0.0 40-41 0.0 0.0 0.0 0.2523927261403376 0.0 42-43 0.0 0.0 0.0 0.3204435327752565 0.0 44-45 0.0 0.0 0.0 0.3933948316142222 0.0 46-47 0.0 0.0 0.0 0.4941155862761194 0.0 48-49 0.0 0.0 0.0 0.6148901281843642 0.0 50-51 0.0 0.0 0.0 0.7347262779684297 0.0 52-53 0.0 0.0 0.0 0.8449699749275527 0.0 54-55 0.0 0.0 0.0 0.9564301061217226 0.0 56-57 0.0 0.0 0.0 1.0781430401541465 0.0 58-59 0.0 0.0 0.0 1.2508419462669718 0.0 60-61 0.0 0.0 0.0 1.5035474697820357 0.0 62-63 0.0 0.0 0.0 1.7201075188842725 0.0 64-65 0.0 0.0 0.0 1.8946832092454562 0.0 66-67 0.0 0.0 0.0 2.0845512157043746 0.0 68-69 0.0 0.0 0.0 2.296871122615876 0.0 70-71 0.0 0.0 0.0 2.557222804179807 0.0 72-73 0.0 0.0 0.0 2.8402696730433306 0.0 74-75 0.0 0.0 0.0 3.109379681099601 0.0 76-77 0.0 0.0 0.0 3.3699051389828245 0.0 78-79 0.0 0.0 0.0 3.655558652635735 0.0 80-81 0.0 0.0 0.0 3.974021135371058 0.0 82-83 0.0 0.0 0.0 4.3021108261951815 0.0 84-85 0.0 0.0 0.0 4.736760156009428 0.0 86-87 0.0 0.0 0.0 5.2085976181522575 0.0 88-89 0.0 0.0 0.0 5.581799641464698 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 145 1.4104444E-8 31.138985 1 GTCGGGA 455 0.0 24.695723 2 CATGGGG 4415 0.0 24.52109 4 TACATGG 15330 0.0 24.506962 2 GTACATG 15355 0.0 24.340885 1 CATGGGT 1560 0.0 24.258532 4 GAGTACT 12800 0.0 23.970987 12-13 ACATGGG 15570 0.0 23.801638 3 GCTACGT 145 1.8062214E-5 23.704844 8 CGGGAGT 460 0.0 22.910093 4 AGTACTT 13160 0.0 22.841656 12-13 GTACTTT 13975 0.0 22.082357 14-15 CATTCCG 245 5.0549716E-9 22.074242 9 ATGGGAG 2070 0.0 21.955976 5 CATGGGA 9245 0.0 21.503204 4 ATGGGGA 2050 0.0 21.226767 5 ACTTTTT 16035 0.0 20.898087 16-17 TCGGGAG 505 0.0 20.885614 3 ATGGGAT 2755 0.0 20.884333 5 ATGGGGT 1385 0.0 20.596691 5 >>END_MODULE