##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939469_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1438631 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96402204595897 31.0 31.0 31.0 30.0 34.0 2 31.15527331191946 31.0 31.0 34.0 28.0 34.0 3 31.13013760999172 31.0 31.0 34.0 28.0 34.0 4 35.010829740218306 35.0 35.0 37.0 32.0 37.0 5 34.68287698513378 35.0 35.0 37.0 32.0 37.0 6 34.70923676745461 35.0 35.0 37.0 32.0 37.0 7 34.59885335433478 35.0 35.0 37.0 30.0 37.0 8 34.65840371853519 35.0 35.0 37.0 31.0 37.0 9 35.90389126885212 37.0 35.0 39.0 30.0 39.0 10-11 35.764156687851155 37.0 35.0 39.0 30.0 39.0 12-13 35.71863632856514 37.0 35.0 39.0 30.0 39.0 14-15 36.28534280159401 38.0 34.5 40.0 30.0 41.0 16-17 36.352089590728966 38.0 34.5 40.0 30.0 41.0 18-19 36.563509683859166 38.0 35.0 40.0 30.0 41.0 20-21 36.57261521543745 38.0 35.0 40.0 30.0 41.0 22-23 36.448222998114176 38.0 35.0 40.0 30.0 41.0 24-25 36.19700013415532 38.0 34.5 40.0 29.5 41.0 26-27 35.57959302976232 38.0 34.0 40.0 28.0 41.0 28-29 35.25083430010892 38.0 33.5 40.0 27.0 41.0 30-31 35.05865611126133 37.0 33.5 40.0 27.0 41.0 32-33 34.80245698862321 37.0 33.0 39.5 27.0 41.0 34-35 34.685782177639716 37.0 33.0 40.0 25.5 41.0 36-37 34.46795529916984 37.0 33.0 40.0 25.0 41.0 38-39 34.23843223175366 37.0 33.0 40.0 24.5 41.0 40-41 33.88920682231928 37.0 33.0 40.0 24.0 41.0 42-43 33.631404786912 37.0 33.0 40.0 23.0 41.0 44-45 33.3637447684639 37.0 33.0 40.0 21.5 41.0 46-47 32.767191517491284 37.0 31.5 40.0 17.0 41.0 48-49 32.28927396948905 36.5 31.0 40.0 11.5 41.0 50-51 31.984759816798054 36.0 31.0 40.0 9.0 41.0 52-53 31.389498766535688 36.0 30.0 39.0 8.0 40.0 54-55 31.226521255276715 35.0 29.5 39.0 6.5 40.0 56-57 31.030979104440263 35.0 29.0 39.0 2.0 40.0 58-59 30.73490492002466 35.0 28.5 39.0 2.0 40.0 60-61 30.45937248675998 35.0 28.0 39.0 2.0 40.0 62-63 29.9700771080284 35.0 28.0 38.0 2.0 40.0 64-65 29.355405938006342 34.0 26.5 38.0 2.0 40.0 66-67 27.811113134639804 33.0 23.0 37.0 2.0 39.5 68-69 24.57828240876222 29.5 12.5 35.0 2.0 39.0 70-71 24.029893349997323 28.5 10.5 35.0 2.0 38.0 72-73 25.60474332890088 31.0 19.0 35.0 2.0 38.0 74-75 26.114166523590832 31.0 21.0 35.0 2.0 37.5 76-77 25.92183541158226 30.5 22.5 34.0 2.0 36.5 78-79 26.63392975683132 32.0 24.5 35.0 2.0 37.0 80-81 26.5876635495829 32.0 24.5 35.0 2.0 36.5 82-83 26.24917960199662 32.0 24.0 35.0 2.0 36.0 84-85 25.928712783194577 32.0 22.5 35.0 2.0 36.0 86-87 25.349198995433852 32.0 20.0 34.5 2.0 35.0 88-89 24.649622106016068 31.0 17.5 34.0 2.0 35.0 90-91 24.08444347438641 31.0 12.5 34.0 2.0 35.0 92-93 23.699957459557037 31.0 4.5 34.0 2.0 35.0 94-95 22.541671213813686 30.0 2.0 34.0 2.0 35.0 96-97 21.663136690367438 29.0 2.0 34.0 2.0 35.0 98-99 21.28941333809712 29.0 2.0 34.0 2.0 35.0 100-101 20.102329575825905 27.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24052988062286929 1101 2 0.1420043189802982 1101 3 0.19958393838399502 1101 4 0.32408277372984884 1101 5 0.2338292361831904 1101 6 0.45152970844980445 1101 7 0.4509848910129364 1101 8 0.35672431507376245 1101 9 0.5423524532488528 1101 10-11 0.5969100734563497 1101 12-13 0.40834078263746676 1101 14-15 0.6525248399229326 1101 16-17 0.6493892008789288 1101 18-19 0.655072743029244 1101 20-21 0.7051664473741752 1101 22-23 0.7763484078143463 1101 24-25 0.7711488668101012 1101 26-27 0.7075571381322234 1101 28-29 0.5157082556506367 1101 30-31 0.5062873470893408 1101 32-33 0.5916485003282261 1101 34-35 0.7385290564906555 1101 36-37 0.46399085721285616 1101 38-39 0.567981541437625 1101 40-41 0.8037936383413111 1101 42-43 0.6513298132927758 1101 44-45 0.40228212122205065 1101 46-47 0.47728383307084243 1101 48-49 0.21019058262464796 1101 50-51 0.33366606742390914 1101 52-53 0.4959179482698879 1101 54-55 0.22246290887141384 1101 56-57 0.2233012099995193 1101 58-59 0.296110410770023 1101 60-61 0.26860773380315806 1101 62-63 0.25401297815805535 1101 64-65 0.3721600865310606 1101 66-67 -2.12647653755576 1101 68-69 -7.2188927195200066 1101 70-71 -10.879512072753501 1101 72-73 -10.657316415223086 1101 74-75 -8.90017467569269 1101 76-77 -4.1115207646691125 1101 78-79 -1.4623706093602244 1101 80-81 -0.9033085197142157 1101 82-83 -0.5851552758255529 1101 84-85 -0.6698799612449164 1101 86-87 -0.6687339861554946 1101 88-89 -0.5245126250381986 1101 90-91 -0.7845793249301458 1101 92-93 -1.3493626581591052 1101 94-95 -4.372412544229899 1101 96-97 -1.815485038591941 1101 98-99 -1.2598207199935416 1101 100-101 -1.3554274563301476 1102 1 0.384967643397772 1102 2 0.3653073995106979 1102 3 0.4002226835327676 1102 4 0.21014290201790686 1102 5 0.29285252208228485 1102 6 0.34746832018977614 1102 7 0.3514403675114224 1102 8 0.5244176567619903 1102 9 0.6789238134795568 1102 10-11 0.5076824126920059 1102 12-13 0.5596730177064657 1102 14-15 0.5689325217092644 1102 16-17 0.5583953895647795 1102 18-19 0.5481903145848719 1102 20-21 0.6268195204929796 1102 22-23 0.6531634075448309 1102 24-25 0.5778059152415338 1102 26-27 0.6078269035214063 1102 28-29 0.5573104919324408 1102 30-31 0.44614558339809207 1102 32-33 0.5171261274034649 1102 34-35 0.6321730200794846 1102 36-37 0.9472987552831498 1102 38-39 0.8528615408986013 1102 40-41 0.8591515512347954 1102 42-43 1.027852139052797 1102 44-45 0.8240004669529668 1102 46-47 0.8326544635958584 1102 48-49 0.8991350023637636 1102 50-51 0.7323503400090736 1102 52-53 0.9489583044309491 1102 54-55 0.8783545411752058 1102 56-57 0.9470439331560527 1102 58-59 0.8554529007980527 1102 60-61 0.5772916021192387 1102 62-63 0.45963686641808366 1102 64-65 0.40110238385749497 1102 66-67 -2.1448168782198387 1102 68-69 -6.799040749382019 1102 70-71 -10.22992661700749 1102 72-73 -8.415464121669832 1102 74-75 -6.657483944908947 1102 76-77 -3.4189602422186987 1102 78-79 -1.0267361427259019 1102 80-81 -0.5190760245654431 1102 82-83 -0.2857535940661755 1102 84-85 -0.2923473838947679 1102 86-87 -0.2463481753264709 1102 88-89 -0.20500360983768218 1102 90-91 -0.3391724954777935 1102 92-93 -0.6870292507097737 1102 94-95 -3.447094519219121 1102 96-97 -0.5450273386135045 1102 98-99 0.010031839703525947 1102 100-101 0.08906808323432003 1103 1 0.34229167909225566 1103 2 0.21159934449050155 1103 3 0.2773900608329747 1103 4 0.17989697441011998 1103 5 0.5035996443464583 1103 6 0.49565438957225183 1103 7 0.4805342107408279 1103 8 0.3878547359921285 1103 9 0.765978622466541 1103 10-11 0.6811402499019223 1103 12-13 0.5944191202225113 1103 14-15 0.7258998930691902 1103 16-17 0.664143930895932 1103 18-19 0.702924305529244 1103 20-21 0.6792111433775787 1103 22-23 0.8091795090048208 1103 24-25 0.9204433502284672 1103 26-27 0.7991480713805217 1103 28-29 0.8627201072662842 1103 30-31 0.8609854763410354 1103 32-33 0.7838187277942055 1103 34-35 0.7551970518137807 1103 36-37 0.7557771029611615 1103 38-39 0.8683376213695908 1103 40-41 1.1486065635113718 1103 42-43 1.0815155594662471 1103 44-45 0.794262350813888 1103 46-47 0.5343562714211885 1103 48-49 0.367809098781116 1103 50-51 0.34882603766200404 1103 52-53 0.3152937454637659 1103 54-55 0.2775822222217528 1103 56-57 0.382102230407682 1103 58-59 0.4163139609400943 1103 60-61 0.22078938771472068 1103 62-63 0.2951880419335673 1103 64-65 0.30493006952425716 1103 66-67 -2.515719734834672 1103 68-69 -7.066602115778512 1103 70-71 -10.388613369522208 1103 72-73 -8.247372750257103 1103 74-75 -6.627381574077045 1103 76-77 -3.339750728529655 1103 78-79 -1.0180014554486618 1103 80-81 -0.3530055860407444 1103 82-83 0.036337071113223374 1103 84-85 -0.2252185029115843 1103 86-87 0.034025633317295245 1103 88-89 0.18937449230874037 1103 90-91 0.18698742677053914 1103 92-93 0.14291784779327443 1103 94-95 0.8829020816204398 1103 96-97 1.001930990319618 1103 98-99 0.5897421520302686 1103 100-101 0.569450307275293 1104 1 0.4002995223323822 1104 2 0.23998190574122447 1104 3 0.3270742982811683 1104 4 0.24676781014801463 1104 5 0.5374924752577428 1104 6 0.6432020654549433 1104 7 0.58929358852793 1104 8 0.37118949020229763 1104 9 0.47183744414430606 1104 10-11 0.6757070354850541 1104 12-13 0.7251269464121037 1104 14-15 0.8398850919045131 1104 16-17 0.8156148663138012 1104 18-19 0.8668462206963383 1104 20-21 0.98746162866167 1104 22-23 0.7359319419831465 1104 24-25 0.9740303537959605 1104 26-27 0.9402911449338589 1104 28-29 1.0357173267196202 1104 30-31 1.0323424027877053 1104 32-33 0.8622634250818635 1104 34-35 0.9439578067584264 1104 36-37 1.02786490659161 1104 38-39 0.9901209748137987 1104 40-41 1.077036997381576 1104 42-43 1.0444577254071703 1104 44-45 1.0426761604251382 1104 46-47 1.0340357543968963 1104 48-49 0.9230544295612404 1104 50-51 0.8036093648746352 1104 52-53 1.0703400441889066 1104 54-55 0.8320496229352514 1104 56-57 0.8976665698966926 1104 58-59 0.7491868755300928 1104 60-61 0.7955682227705942 1104 62-63 0.7693015787364637 1104 64-65 0.68481406039567 1104 66-67 -2.321661841754562 1104 68-69 -7.016654742870479 1104 70-71 -8.155207298224795 1104 72-73 -7.3566690555401415 1104 74-75 -6.354609290356358 1104 76-77 -3.223733402576137 1104 78-79 -0.45916895715371453 1104 80-81 0.13431800663540017 1104 82-83 0.21545526402020343 1104 84-85 0.34897012176707776 1104 86-87 0.3055699515587307 1104 88-89 0.2739590169926487 1104 90-91 0.3312291048042191 1104 92-93 0.2836289201399502 1104 94-95 1.1565613203802094 1104 96-97 1.2875067405425895 1104 98-99 0.7214672713839221 1104 100-101 0.4140970991540023 1105 1 0.4241051700841183 1105 2 0.3517431146639787 1105 3 0.4432236674325125 1105 4 0.23960242558870704 1105 5 0.4691001876639689 1105 6 0.35481740733133904 1105 7 0.5067701777174065 1105 8 0.4722220604266312 1105 9 0.7813635098808618 1105 10-11 0.7837251874686331 1105 12-13 0.641638917876918 1105 14-15 0.9949684491305533 1105 16-17 0.9960811954047557 1105 18-19 0.9088060793162569 1105 20-21 0.9375015578420474 1105 22-23 1.0782926717557402 1105 24-25 1.057927798166439 1105 26-27 0.9047323064923418 1105 28-29 0.9205078726559961 1105 30-31 0.9017859293199564 1105 32-33 1.0451273273209765 1105 34-35 1.0823067472987802 1105 36-37 0.9628499959179635 1105 38-39 0.998646219288915 1105 40-41 1.1219253333633574 1105 42-43 1.21016908845818 1105 44-45 1.0108079364394413 1105 46-47 1.1813829341656827 1105 48-49 0.8921068850033578 1105 50-51 0.9166314206847765 1105 52-53 1.0707711254516745 1105 54-55 1.2516016385894346 1105 56-57 1.200882731738652 1105 58-59 1.3473654812646707 1105 60-61 1.292898110693514 1105 62-63 1.3695845656659316 1105 64-65 1.262612788169374 1105 66-67 1.9668402551348514 1105 68-69 -4.752311217472116 1105 70-71 -4.365005781401894 1105 72-73 -1.0299953101506212 1105 74-75 1.0912048680612472 1105 76-77 1.3275231517218806 1105 78-79 1.3447204527853316 1105 80-81 1.3537926341925264 1105 82-83 1.3841657701731869 1105 84-85 1.3557617270821147 1105 86-87 1.7235112337956728 1105 88-89 1.5714551010172535 1105 90-91 1.494755701849943 1105 92-93 1.467640482593886 1105 94-95 2.2668832969799304 1105 96-97 2.483381238188148 1105 98-99 2.001035922846725 1105 100-101 1.7695060857303453 1106 1 0.17221891020775004 1106 2 0.06974111420954188 1106 3 0.09677260009403099 1106 4 0.02508765291200632 1106 5 0.12462849268876397 1106 6 0.18643957911275066 1106 7 0.20055893066597008 1106 8 0.13676178022246432 1106 9 0.2795809035276591 1106 10-11 0.2989060365139622 1106 12-13 0.4113055634615108 1106 14-15 0.3671326175716274 1106 16-17 0.34419950331077587 1106 18-19 0.4312971370038454 1106 20-21 0.4107612913178045 1106 22-23 0.5124925953917909 1106 24-25 0.5777882816954829 1106 26-27 0.5556817024909648 1106 28-29 0.6407844928718305 1106 30-31 0.32540909430123577 1106 32-33 0.2634884070041821 1106 34-35 0.4130481569398441 1106 36-37 0.4234786398956345 1106 38-39 0.30572066008694065 1106 40-41 0.5146052864206112 1106 42-43 0.4360927760406099 1106 44-45 0.599380750404201 1106 46-47 0.1857369677517653 1106 48-49 0.11170419399392273 1106 50-51 0.1250253649765689 1106 52-53 0.4210251644254015 1106 54-55 0.26810480207153375 1106 56-57 0.17991134011104037 1106 58-59 0.384833594629999 1106 60-61 0.4343184099183972 1106 62-63 0.40133475247651873 1106 64-65 0.1940989805831066 1106 66-67 0.9357523967688977 1106 68-69 -5.4353047780701935 1106 70-71 -5.851865794097986 1106 72-73 -2.1606321292875244 1106 74-75 0.19924229108240255 1106 76-77 0.30827404829557636 1106 78-79 0.241240856717841 1106 80-81 0.3506461348706651 1106 82-83 0.42452564115090397 1106 84-85 0.500559842813324 1106 86-87 0.6324841152230682 1106 88-89 0.5577025623843568 1106 90-91 0.33045901150108037 1106 92-93 0.45872042347038544 1106 94-95 1.1264257457329236 1106 96-97 1.3079321314824064 1106 98-99 1.207959978194193 1106 100-101 1.1494565662844742 1107 1 -0.16918791274447642 1107 2 -0.023732288162559456 1107 3 -0.03702320447314733 1107 4 0.05298370910399797 1107 5 0.09352311373421429 1107 6 0.16019520589878056 1107 7 0.0455852311489906 1107 8 0.07216596048192514 1107 9 0.3559020918542899 1107 10-11 0.1489336172488649 1107 12-13 0.22005687532454488 1107 14-15 0.06167795429367828 1107 16-17 0.044883726814298086 1107 18-19 -0.008491510797291824 1107 20-21 0.04687695970411454 1107 22-23 0.08915399880073238 1107 24-25 0.25941018696315865 1107 26-27 0.1786123570948135 1107 28-29 0.031406331756080874 1107 30-31 0.180181904912466 1107 32-33 0.07814270738604279 1107 34-35 0.2896358273239841 1107 36-37 0.3383663886754462 1107 38-39 0.23823558055326544 1107 40-41 0.02934635942974495 1107 42-43 0.24095433497645047 1107 44-45 0.12111184060980662 1107 46-47 0.0627363734620019 1107 48-49 0.31583205796478353 1107 50-51 0.24327756827424807 1107 52-53 0.3098728270964166 1107 54-55 0.20200824262991546 1107 56-57 0.29409202632604803 1107 58-59 0.4934823282870333 1107 60-61 0.3658786734290089 1107 62-63 0.5324329398927503 1107 64-65 0.41717496748344374 1107 66-67 1.3305812855734906 1107 68-69 -5.05257150353361 1107 70-71 -5.17847306339976 1107 72-73 -1.9696304033183232 1107 74-75 0.17108781367805648 1107 76-77 0.024407434735653766 1107 78-79 0.14685823842888723 1107 80-81 0.26210920987762165 1107 82-83 0.1954569690724064 1107 84-85 0.16923302770065973 1107 86-87 0.4106200210723969 1107 88-89 0.372729084145476 1107 90-91 0.3499338553419662 1107 92-93 0.4366040722830711 1107 94-95 1.4013969795796193 1107 96-97 1.4815228270543166 1107 98-99 1.1318339887649636 1107 100-101 1.2744885725814186 1108 1 0.02233214355390345 1108 2 -0.1886309670698587 1108 3 0.0017195183212983522 1108 4 -0.03553549951988799 1108 5 0.010239894490403856 1108 6 0.18997137185105828 1108 7 0.05011629122191863 1108 8 -0.10001672136824169 1108 9 0.05910606152993836 1108 10-11 0.11861465972855001 1108 12-13 0.15022908919441846 1108 14-15 0.26724021838426637 1108 16-17 0.1599591378564611 1108 18-19 0.16803990114052425 1108 20-21 0.147120266274392 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1.6236642669636119 2316 98-99 0.5968850091731248 2316 100-101 0.6413607381142974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 16.0 4 49.0 5 201.0 6 466.0 7 1028.0 8 1941.0 9 3356.0 10 5508.0 11 8113.0 12 10496.0 13 13246.0 14 16726.0 15 21654.0 16 26069.0 17 30095.0 18 24995.0 19 17690.0 20 16588.0 21 16692.0 22 17356.0 23 19333.0 24 22961.0 25 27900.0 26 34459.0 27 42104.0 28 51306.0 29 60468.0 30 71254.0 31 82284.0 32 94347.0 33 109686.0 34 127687.0 35 144398.0 36 142311.0 37 117069.0 38 54480.0 39 4294.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.48801457843312 20.293904771572333 15.29671714099183 17.92136350900271 2 14.0537075686431 24.262052778747233 40.088724692049816 21.59551496055985 3 21.568908218994306 30.486761372443667 31.534563067249348 16.40976734131268 4 11.15963718284953 18.395196544492645 39.791440612637984 30.653725660019838 5 10.575492761158548 43.06276535699291 32.944374779303615 13.417367102544924 6 24.25291822572988 39.10182666715787 19.332406989700626 17.312848117411622 7 23.922465176963378 39.34851953002542 19.894955690514106 16.834059602497096 8 23.33885478625165 33.451524400628095 20.34169985215111 22.867920960969144 9 26.770033455416993 20.08430236801515 22.16565609944454 30.980008077123323 10-11 22.618621453312212 27.770707012430567 26.721654128125977 22.889017406131245 12-13 29.70271737506004 26.002845761004735 26.465577344016637 17.828859519918588 14-15 25.760740592966506 26.45727083595446 29.978118085874694 17.80387048520434 16-17 17.782459715888535 30.55124492093321 32.049133009250845 19.617162353927412 18-19 16.83086383023684 30.965942266734135 35.26201571510396 16.94117818792506 20-21 18.84812714309646 28.962291233818817 32.89915899212515 19.290422630959572 22-23 18.10373890177537 28.05788975769325 31.78487047755818 22.053500862973202 24-25 18.6572859892495 28.449268784003685 34.33976467905946 18.55368054768735 26-27 21.06356305823645 26.10257075064306 35.07366227439853 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18.414197890489813 30.125301079102286 33.440831978296224 18.019669052111684 54-55 16.513360406677474 29.94217498162864 34.27677341475044 19.267691196943453 56-57 18.76819671469985 32.75415599578055 29.69710792620556 18.780539363314034 58-59 17.600072580342406 30.361803273901806 31.074571435324938 20.963552710430854 60-61 18.18362245273916 28.468633429405234 32.180401054983435 21.167343062872174 62-63 22.886198782848663 27.771208914191174 30.678678302939304 18.663914000020853 64-65 21.415440761176523 28.351566233653386 32.64439327453621 17.58859973063388 66-67 17.879231844677 29.492230988239516 33.827954482476535 18.80058268460695 68-69 18.156176307679047 32.158904073115046 31.659561140168112 18.02535847903779 70-71 17.922974914742664 31.528383697633274 29.97429131044891 20.574350077175158 72-73 17.905831856242017 30.472039584885646 28.42054968010489 23.201578878767453 74-75 18.599049874125203 30.63273621842384 30.565561226176808 20.202652681274152 76-77 20.526433243234223 28.69521056495478 31.249513353577825 19.528842838233167 78-79 18.068850599163298 28.480833587068805 33.5529361712777 19.897379642490197 80-81 17.324059579650385 32.74030801911843 31.390048185334194 18.54558421589699 82-83 17.25963780844428 32.186884614609305 29.403266021655313 21.150211555291108 84-85 18.076629795965747 29.272064900589516 29.740948165304378 22.91035713814036 86-87 19.676392950920235 29.521227697927365 30.95419071302335 19.84818863812905 88-89 21.396191698922685 26.977197947617178 31.82892917207839 19.797681181381748 90-91 18.031694613931517 28.737689619397006 33.69138503620776 19.539230730463704 92-93 16.91103918243674 31.634629520793066 31.57912480497756 19.875206491792635 94-95 16.768144433747093 32.25829598161975 29.736654744770647 21.23690483986251 96-97 16.819407064423377 30.18048773350853 28.131799380352852 24.86830582171524 98-99 18.458449084695236 29.46775924829049 29.000478299921863 23.07331336709241 100-101 20.59839475242817 29.787102407176203 29.698887476955676 19.91561536343995 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9978.0 1 6444.5 2 2926.5 3 3030.5 4 2661.0 5 2549.0 6 4488.5 7 8513.0 8 11401.0 9 11762.0 10 10299.5 11 8214.0 12 7193.0 13 6652.0 14 6282.5 15 6015.5 16 5859.0 17 5969.0 18 6141.5 19 6497.5 20 7153.0 21 7817.5 22 8502.5 23 9377.5 24 10392.0 25 11859.0 26 14117.5 27 16326.0 28 18167.0 29 19925.5 30 22288.5 31 25156.0 32 28932.5 33 33711.5 34 38338.0 35 41762.0 36 45408.5 37 48844.0 38 47932.5 39 44873.0 40 41817.5 41 38507.0 42 35950.5 43 35253.0 44 37689.5 45 40112.0 46 46446.5 47 69406.0 48 127103.5 49 118892.0 50 55455.0 51 33157.0 52 21485.5 53 15317.5 54 11821.5 55 9877.5 56 8723.0 57 7860.0 58 7541.0 59 7537.0 60 7494.0 61 7163.0 62 5903.5 63 4075.5 64 2713.5 65 2115.0 66 1747.0 67 1513.0 68 1355.0 69 1143.5 70 988.5 71 884.0 72 733.5 73 594.0 74 455.5 75 342.0 76 255.5 77 172.0 78 105.5 79 61.0 80 38.0 81 23.5 82 13.5 83 8.0 84 3.5 85 1.5 86 1.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02377260047920558 2 0.017308121401526867 3 0.0 4 0.0 5 2.0853158315092612E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.8230790244336453E-4 18-19 0.0010426579157546305 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.127973747263892E-4 28-29 0.01800322667869662 30-31 0.004761471148612814 32-33 0.009210144922499237 34-35 0.010461334421404794 36-37 0.100268936231737 38-39 0.10297984681269902 40-41 0.11709048393924502 42-43 0.10138110467520858 44-45 0.09651536773502031 46-47 0.0973842493314825 48-49 0.09971285201000119 50-51 0.09787082302550132 52-53 0.07600976205851258 54-55 0.06474905656836256 56-57 0.036701558634562995 58-59 0.01563986873631946 60-61 0.008688815964621921 62-63 0.0018072737206413596 64-65 0.004761471148612814 66-67 0.4137266609714374 68-69 0.7962778502618114 70-71 0.8795862177306064 72-73 0.2740105002603169 74-75 0.14489469502603516 76-77 0.014944763459149706 78-79 0.008688815964621921 80-81 0.001320700026622532 82-83 0.0 84-85 0.0 86-87 2.432868470094138E-4 88-89 7.298605410282414E-4 90-91 0.00406636587144306 92-93 0.001007902651896143 94-95 0.22083494655683075 96-97 0.2292457204105848 98-99 0.014110637126546 100-101 0.006881542243980562 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1438631.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.102497403592935 #Duplication Level Percentage of deduplicated Percentage of total 1 76.67713807031214 28.44913316168693 2 12.093537277124979 8.974008708495678 3 4.3458998318120825 4.837312116782483 4 2.0791103023184068 3.08560738374208 5 1.1404903000397129 2.1157519198023187 6 0.7476426602831527 1.6643645917182592 7 0.4935969599027573 1.2819575946249393 8 0.344950626628916 1.023882378309369 9 0.2594837735864964 0.8664746432190731 >10 1.5298340157071184 10.963751009777836 >50 0.1582808242142276 4.0582022835598375 >100 0.1113499261323855 7.876961830518969 >500 0.008389377604667977 2.04615515491665 >1k 0.006673368549167709 5.319479908517407 >5k 0.001525341382666905 3.7003970351051065 >10k+ 0.0020973444011669943 13.736560279223145 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 34058 2.367389552984747 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 30009 2.0859414262587137 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19921 1.3847192226498666 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 18965 1.318267158152438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17645 1.2265132615660306 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 13507 0.9388786978731865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12960 0.9008564392120009 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 12799 0.8896652442495678 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 11849 0.8236302429184413 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11731 0.8154280006478382 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 10733 0.7460564939862967 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 8479 0.5893797645122342 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8419 0.5852091328492156 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 7355 0.5112499313583538 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5963 0.41449127677632414 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5673 0.39433322373840135 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5645 0.392386928962326 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5393 0.37487027597764816 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5381 0.3740361496450445 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4599 0.31967891697036976 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4555 0.3166204537508228 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4370 0.3037610061231824 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4367 0.3035524745400315 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3702 0.25732797360824283 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2845 0.19775745135479494 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2779 0.19316975652547455 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2660 0.18489800372715448 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2561 0.17801646148317393 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2383 0.16564358754955233 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2275 0.15813645055611897 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2226 0.1547304346979872 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2174 0.15111588725670447 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2146 0.14916959248062917 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2068 0.1437477713187051 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1999 0.13895154490623376 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1921 0.1335297237443097 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1883 0.13088832369106462 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1811 0.1258835656954424 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1649 0.1146228602052924 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1626 0.11302411806780195 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1623 0.11281558648465104 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1526 0.10607306529610441 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1509 0.10489138632491585 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1479 0.10280607049340659 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 6.951052771697537E-5 16-17 0.0 0.0 0.0 3.475526385848769E-5 6.951052771697537E-5 18-19 0.0 0.0 0.0 1.3902105543395075E-4 6.951052771697537E-5 20-21 0.0 0.0 0.0 3.4755263858487687E-4 6.951052771697537E-5 22-23 0.0 0.0 0.0 7.646158048867292E-4 6.951052771697537E-5 24-25 0.0 0.0 0.0 0.0020505605676507735 6.951052771697537E-5 26-27 0.0 0.0 0.0 0.00375356849671667 6.951052771697537E-5 28-29 0.0 0.0 0.0 0.007611402785008803 6.951052771697537E-5 30-31 0.0 0.0 0.0 0.019011129330592763 6.951052771697537E-5 32-33 0.0 0.0 0.0 0.03885638499378924 6.951052771697537E-5 34-35 0.0 0.0 0.0 0.07726095155741813 6.951052771697537E-5 36-37 0.0 0.0 0.0 0.13450287113234735 6.951052771697537E-5 38-39 0.0 0.0 0.0 0.20620298047240743 6.951052771697537E-5 40-41 0.0 0.0 0.0 0.28912904003875906 6.951052771697537E-5 42-43 0.0 0.0 0.0 0.3659729284298754 6.951052771697537E-5 44-45 0.0 0.0 0.0 0.44917703010709487 6.951052771697537E-5 46-47 0.0 0.0 0.0 0.5647382824365664 6.951052771697537E-5 48-49 0.0 0.0 0.0 0.6970168166819706 6.951052771697537E-5 50-51 0.0 0.0 0.0 0.8293996167189501 6.951052771697537E-5 52-53 0.0 0.0 0.0 0.9505217112657798 6.951052771697537E-5 54-55 0.0 0.0 0.0 1.0731035268946658 6.951052771697537E-5 56-57 0.0 0.0 0.0 1.2010028978939005 6.951052771697537E-5 58-59 0.0 0.0 0.0 1.3629971827383116 6.951052771697537E-5 60-61 0.0 0.0 0.0 1.5769505870511618 6.951052771697537E-5 62-63 0.0 0.0 0.0 1.7604931354878355 6.951052771697537E-5 64-65 0.0 0.0 0.0 1.9133467859374642 6.951052771697537E-5 66-67 0.0 0.0 0.0 2.0635937915977065 6.951052771697537E-5 68-69 0.0 0.0 0.0 2.2377176635287297 6.951052771697537E-5 70-71 0.0 0.0 0.0 2.4918829081258504 6.951052771697537E-5 72-73 0.0 0.0 0.0 2.7853216008830617 6.951052771697537E-5 74-75 0.0 0.0 0.0 3.080741343680207 6.951052771697537E-5 76-77 0.0 0.0 0.0 3.3736934627434 1.3902105543395075E-4 78-79 0.0 0.0 0.0 3.7001149009023164 1.3902105543395075E-4 80-81 0.0 0.0 0.0 4.063967758236824 1.3902105543395075E-4 82-83 0.0 0.0 0.0 4.437864886826434 1.3902105543395075E-4 84-85 0.0 0.0 0.0 4.929582359896318 1.3902105543395075E-4 86-87 0.0 0.0 0.0 5.450285723024181 1.3902105543395075E-4 88-89 0.0 0.0 0.0 5.863143502399156 1.3902105543395075E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 255 0.0 39.016205 9 GCAACGC 485 0.0 28.290323 68-69 ATGGGCG 220 1.8189894E-12 27.995396 5 GTAGCGA 35 0.0078011234 27.553205 64-65 GGTATCG 275 0.0 27.122795 62-63 TACCTGG 700 0.0 27.076237 2 GGTAGCA 775 0.0 27.06436 64-65 GTAGCAA 775 0.0 26.131105 64-65 GCGTTTA 875 0.0 26.122877 62-63 CGTCACT 130 1.0188751E-5 25.523016 1 TATCACG 95 0.0012444037 24.938639 2 GTATCGA 280 0.0 24.91985 62-63 TAGGCAT 460 0.0 24.718344 5 AGGCTTA 505 0.0 24.39203 6 GGGAACG 370 0.0 24.328638 7 TAGGGTC 275 1.8189894E-12 24.119112 4 CGCATAT 60 1.546074E-4 24.109055 64-65 CATGGGG 4805 0.0 24.058159 4 AGCAACG 565 0.0 23.896547 66-67 TGCGTCG 155 0.0 23.284397 62-63 >>END_MODULE