##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939468_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1440449 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.868357019234974 31.0 31.0 31.0 30.0 34.0 2 31.051766497807282 31.0 31.0 33.0 28.0 34.0 3 31.02720679454809 31.0 31.0 34.0 27.0 34.0 4 34.88557803851438 35.0 35.0 37.0 32.0 37.0 5 34.51472145143632 35.0 35.0 37.0 30.0 37.0 6 34.6318307694337 35.0 35.0 37.0 31.0 37.0 7 34.49548855947 35.0 35.0 37.0 30.0 37.0 8 34.43562389227248 35.0 35.0 37.0 30.0 37.0 9 35.828168855683195 37.0 35.0 39.0 30.0 39.0 10-11 35.63678547452912 37.0 35.0 39.0 30.0 39.0 12-13 35.59760463577676 37.0 35.0 39.0 30.0 39.0 14-15 36.108833426244175 38.0 34.0 40.0 29.5 41.0 16-17 36.09209003581522 38.0 34.0 40.0 29.5 41.0 18-19 36.36154213026633 38.0 35.0 40.0 30.0 41.0 20-21 36.34067814966028 38.0 34.5 40.0 29.5 41.0 22-23 36.19047810786776 38.0 34.0 40.0 29.5 41.0 24-25 35.990470679628366 38.0 34.0 40.0 29.0 41.0 26-27 35.27893143040816 37.5 33.5 40.0 26.5 41.0 28-29 35.029250254608115 37.0 33.0 40.0 26.5 41.0 30-31 34.76516940204061 37.0 33.0 39.0 27.0 40.0 32-33 34.462123268508634 37.0 33.0 39.0 25.0 40.5 34-35 34.618637313782024 37.0 33.0 40.0 26.0 41.0 36-37 34.25412805312788 37.0 33.0 40.0 24.5 41.0 38-39 33.86830877039034 36.5 33.0 40.0 24.0 41.0 40-41 33.57737170840481 36.0 32.5 39.0 23.5 41.0 42-43 33.450279392050675 36.0 33.0 39.5 22.5 41.0 44-45 32.996579538741045 36.0 31.5 40.0 20.0 41.0 46-47 32.31687029530376 36.0 31.0 39.0 15.0 41.0 48-49 31.769523252819084 36.0 30.0 39.0 9.0 40.0 50-51 31.476165764980223 36.0 30.0 39.0 8.0 40.0 52-53 31.10648589432878 35.0 29.0 39.0 7.0 40.0 54-55 30.832164484823828 35.0 28.0 39.0 2.0 40.0 56-57 30.56542092083788 35.0 28.0 39.0 2.0 40.0 58-59 30.22935348630878 35.0 28.0 38.5 2.0 40.0 60-61 29.874947325451995 34.5 27.5 38.0 2.0 40.0 62-63 29.59529771619821 34.0 27.0 38.0 2.0 40.0 64-65 28.943934495424692 34.0 26.0 38.0 2.0 40.0 66-67 28.14504019232892 33.5 24.5 37.0 2.0 39.5 68-69 25.111819995015445 31.0 12.5 36.0 2.0 39.0 70-71 23.910151973447167 29.0 10.0 35.0 2.0 38.0 72-73 24.728442659198624 30.0 15.0 35.0 2.0 37.5 74-75 25.376531206589057 30.5 19.0 35.0 2.0 37.0 76-77 24.80560123961348 30.5 19.0 34.0 2.0 36.0 78-79 25.782296353428688 31.0 21.0 35.0 2.0 36.0 80-81 25.891782701088342 32.0 21.5 35.0 2.0 36.0 82-83 25.64240629137165 32.0 20.5 35.0 2.0 36.0 84-85 25.303409214765672 31.5 20.0 34.5 2.0 35.5 86-87 24.718693268557235 31.0 18.0 34.0 2.0 35.0 88-89 24.02391893083337 31.0 11.5 34.0 2.0 35.0 90-91 23.317122300060603 30.0 6.5 34.0 2.0 35.0 92-93 22.915449280050872 30.0 2.0 34.0 2.0 35.0 94-95 22.218942843516153 29.0 2.0 34.0 2.0 35.0 96-97 21.458450455378845 29.0 2.0 34.0 2.0 35.0 98-99 20.58847137246789 29.0 2.0 34.0 2.0 35.0 100-101 19.272244626501877 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17418380776131315 1101 2 0.06227578392341826 1101 3 0.24295844942889033 1101 4 0.12479803524577449 1101 5 0.5734054064789902 1101 6 0.2887502617382154 1101 7 0.48440721318438307 1101 8 0.6637099406949574 1101 9 0.5266045766427894 1101 10-11 0.5709120272370782 1101 12-13 0.6151876999900452 1101 14-15 0.844388737241097 1101 16-17 0.6495489515984687 1101 18-19 0.4143867631762177 1101 20-21 0.6464564732308631 1101 22-23 0.641019708172557 1101 24-25 0.830205919969984 1101 26-27 0.7057222890003132 1101 28-29 0.6814606609164997 1101 30-31 0.390702407476077 1101 32-33 0.7156325305268965 1101 34-35 0.850313034169794 1101 36-37 0.8674977418148089 1101 38-39 1.058731447888455 1101 40-41 1.1204100357274385 1101 42-43 0.8357927472197773 1101 44-45 0.8446809523470051 1101 46-47 0.8215603716302482 1101 48-49 0.8227750832440748 1101 50-51 0.8016858102898752 1101 52-53 0.33786400207937817 1101 54-55 0.4437807963966556 1101 56-57 0.6017478293391001 1101 58-59 0.7103442508735576 1101 60-61 0.4446567746164831 1101 62-63 0.14472845962585623 1101 64-65 6.152161345696072E-4 1101 66-67 -0.033771184849758384 1101 68-69 -6.760316006139206 1101 70-71 -7.234613197054411 1101 72-73 -4.38508478434915 1101 74-75 -2.716850010879881 1101 76-77 -0.34160324307463696 1101 78-79 0.08811315254015639 1101 80-81 0.0011183188877659234 1101 82-83 -0.19620681756570235 1101 84-85 -0.3093684306057618 1101 86-87 -0.4348669688085174 1101 88-89 -0.15694801745082643 1101 90-91 -0.15760813828594777 1101 92-93 -0.26979776136517586 1101 94-95 -0.18881655702627143 1101 96-97 -0.3865294572651159 1101 98-99 -0.5670180786227945 1101 100-101 -0.4425880984866879 1102 1 0.3008103533970612 1102 2 0.3417256994175304 1102 3 0.34487922451994635 1102 4 0.4402104701967957 1102 5 0.383305694672309 1102 6 0.3416974365703638 1102 7 0.27483647225528074 1102 8 0.39455356114281415 1102 9 0.46780748837138475 1102 10-11 0.5863683846995471 1102 12-13 0.45409289928522867 1102 14-15 0.7063083051052317 1102 16-17 0.5011460871315236 1102 18-19 0.4200543082931105 1102 20-21 0.44142182324961965 1102 22-23 0.48769225685976636 1102 24-25 0.6045129203449946 1102 26-27 0.5618253348081552 1102 28-29 0.5167705072647593 1102 30-31 0.4953497174148893 1102 32-33 0.7844630872383078 1102 34-35 0.7381700008965595 1102 36-37 0.7459845727670853 1102 38-39 0.8408250619148916 1102 40-41 0.7637752963600306 1102 42-43 0.5891376647944284 1102 44-45 0.6503829162740402 1102 46-47 0.7673989063136233 1102 48-49 0.781961181127059 1102 50-51 0.6418001629337056 1102 52-53 0.2753399217551262 1102 54-55 0.5356083838435524 1102 56-57 0.4938461438448307 1102 58-59 0.7256452099768218 1102 60-61 0.6952899266089219 1102 62-63 0.24444026630515836 1102 64-65 0.23975145811422038 1102 66-67 0.21797351491033723 1102 68-69 -6.4734031165516654 1102 70-71 -7.2965811824238465 1102 72-73 -1.1898280302998039 1102 74-75 0.4105817807989851 1102 76-77 0.8065023096554924 1102 78-79 0.5551398040009019 1102 80-81 0.0983045811590948 1102 82-83 0.35627352004686585 1102 84-85 0.07483089540201249 1102 86-87 -0.12182789061617072 1102 88-89 -0.2413973935456788 1102 90-91 -0.23695698063238524 1102 92-93 0.03347947444477484 1102 94-95 0.2208835112976324 1102 96-97 -0.2667436501440186 1102 98-99 -0.29238101726998167 1102 100-101 -0.35697708754972624 1103 1 0.3671372685648713 1103 2 0.2943948009562867 1103 3 0.40904121724809883 1103 4 0.2417610216478039 1103 5 0.5182592174537604 1103 6 0.3798422639418604 1103 7 0.3877086054227945 1103 8 0.5991037078741712 1103 9 0.569753419791887 1103 10-11 0.6536071479942578 1103 12-13 0.6459507017722075 1103 14-15 0.6594888362874869 1103 16-17 0.6974412637580585 1103 18-19 0.6841474003676495 1103 20-21 0.5673015150072658 1103 22-23 0.6070642777872095 1103 24-25 0.8135831875418944 1103 26-27 0.8119582614555938 1103 28-29 0.7033466773060013 1103 30-31 0.7955947612376733 1103 32-33 0.8668589702399245 1103 34-35 0.8441649722138962 1103 36-37 0.8191186021526775 1103 38-39 0.9380268023732512 1103 40-41 0.8051929047782025 1103 42-43 0.6832814779089915 1103 44-45 0.8716863265696517 1103 46-47 1.0364807416107524 1103 48-49 0.9629595524171961 1103 50-51 0.8480867536048322 1103 52-53 0.7632573196207595 1103 54-55 0.47894394559279263 1103 56-57 0.7824518109031082 1103 58-59 1.0695160011791636 1103 60-61 1.0278990247722781 1103 62-63 0.6682161532064974 1103 64-65 0.5357588249950425 1103 66-67 0.569510835141898 1103 68-69 -6.6846270412181 1103 70-71 -7.156877119333458 1103 72-73 -0.699565265654936 1103 74-75 0.9187040290740143 1103 76-77 1.0176830178001879 1103 78-79 0.776056723412534 1103 80-81 0.5416157696093471 1103 82-83 0.49071561318481827 1103 84-85 0.30582689080754477 1103 86-87 0.37384554158672856 1103 88-89 0.5537005830199071 1103 90-91 0.050356082308894656 1103 92-93 0.11208889376219133 1103 94-95 0.3032597318059409 1103 96-97 0.3791751945534152 1103 98-99 0.4528735628620524 1103 100-101 0.6638661794390757 1104 1 0.25477079863657437 1104 2 0.21198012199753435 1104 3 0.1416343427866913 1104 4 0.16219417125026325 1104 5 0.3644476147677125 1104 6 0.338422739691012 1104 7 0.4731121629728321 1104 8 0.47963108052265824 1104 9 0.3235816883316289 1104 10-11 0.4940259080547449 1104 12-13 0.30173253355277296 1104 14-15 0.3780808155405353 1104 16-17 0.31390448506433444 1104 18-19 0.39767804114939764 1104 20-21 0.4754657508982021 1104 22-23 0.5246506150390502 1104 24-25 0.5418017297268278 1104 26-27 0.6851457482448495 1104 28-29 0.5499771262271906 1104 30-31 0.48798537632962535 1104 32-33 0.6364582174311124 1104 34-35 0.3881998160426505 1104 36-37 0.5081416985869964 1104 38-39 0.5838831020659541 1104 40-41 0.530459355195255 1104 42-43 0.6371499883881526 1104 44-45 0.6514537769379274 1104 46-47 0.5060616970112974 1104 48-49 0.5556617376764592 1104 50-51 0.5535324229217764 1104 52-53 0.41671589074736914 1104 54-55 0.07068093403238151 1104 56-57 0.2938809281819381 1104 58-59 0.2587002686132749 1104 60-61 0.7338906024188994 1104 62-63 0.47719965807617726 1104 64-65 0.3928050259933684 1104 66-67 0.3627235646022484 1104 68-69 -5.927530029690207 1104 70-71 -3.4542014368464287 1104 72-73 0.6426704789428896 1104 74-75 0.9671734126813192 1104 76-77 0.98993254221363 1104 78-79 0.4190684464179171 1104 80-81 0.23013970340119627 1104 82-83 0.3499999163895424 1104 84-85 0.5125048170875637 1104 86-87 0.3971072117491623 1104 88-89 0.33433059293812306 1104 90-91 0.27522499114261123 1104 92-93 0.08432175232193373 1104 94-95 0.31863743695853586 1104 96-97 0.06163539974665966 1104 98-99 0.4406914335350294 1104 100-101 0.7119207458829919 1105 1 0.06613552425363878 1105 2 0.1137414447868963 1105 3 0.05497606337140226 1105 4 0.06288911493586369 1105 5 0.1530948709383395 1105 6 0.13312079864140713 1105 7 0.14693794777992508 1105 8 0.24684291852889118 1105 9 0.2757743554775658 1105 10-11 0.2111299099718238 1105 12-13 0.38782167805312895 1105 14-15 0.3518436959622946 1105 16-17 0.233024422759037 1105 18-19 0.29556325562339936 1105 20-21 0.19267758890442366 1105 22-23 0.5157990693884429 1105 24-25 0.5004226259645463 1105 26-27 0.5220616360953159 1105 28-29 0.5519331530664005 1105 30-31 0.4801972143358526 1105 32-33 0.40411038613468264 1105 34-35 0.37935126583897016 1105 36-37 0.2793704309138576 1105 38-39 0.4748967613039099 1105 40-41 0.4179054371506794 1105 42-43 0.24016340799035873 1105 44-45 0.33141723248549226 1105 46-47 0.29029066897391687 1105 48-49 0.3684852459438872 1105 50-51 0.19425252836150975 1105 52-53 0.06768941099659287 1105 54-55 0.03694021991783458 1105 56-57 0.011452219818650633 1105 58-59 0.06298974860368745 1105 60-61 0.18129055449174913 1105 62-63 -0.03320951997318744 1105 64-65 0.021528366515774167 1105 66-67 0.06351735803622915 1105 68-69 -2.7911323541570177 1105 70-71 -3.172961920910648 1105 72-73 0.20982240801070517 1105 74-75 0.48470576350806027 1105 76-77 0.46676276028216535 1105 78-79 -0.12424571605512469 1105 80-81 -0.23960927814685107 1105 82-83 -0.30913919216545693 1105 84-85 -0.5429798462242026 1105 86-87 -0.471090128293973 1105 88-89 -0.35353485158620046 1105 90-91 -0.17666513586434007 1105 92-93 -0.03015524264571212 1105 94-95 -0.08052982879751269 1105 96-97 -0.13128336373285165 1105 98-99 -0.12146708551601648 1105 100-101 -0.14036777556441038 1106 1 0.046209689694755696 1106 2 0.14167880740687977 1106 3 0.02668250105820036 1106 4 0.15299854454194417 1106 5 0.30887036629706444 1106 6 0.19707821281349425 1106 7 0.2315138289887102 1106 8 0.3059424172400824 1106 9 0.18482404157906274 1106 10-11 0.34887264760960335 1106 12-13 0.25331557403175253 1106 14-15 0.19610437404632108 1106 16-17 0.027546617044031052 1106 18-19 0.15274279450095918 1106 20-21 0.2708972229794142 1106 22-23 0.2921730672061713 1106 24-25 0.32705813998323663 1106 26-27 0.551177897145017 1106 28-29 0.5993881635823897 1106 30-31 0.4253584727763169 1106 32-33 0.6068587771083216 1106 34-35 0.4354681357094492 1106 36-37 0.5859286958536956 1106 38-39 0.547930349235024 1106 40-41 0.5858332325869 1106 42-43 0.4672668047359352 1106 44-45 0.6862741596009485 1106 46-47 0.6984897849977543 1106 48-49 0.9269083210423261 1106 50-51 0.7654499681515077 1106 52-53 0.4998165675663202 1106 54-55 0.4518503990850924 1106 56-57 0.5653097320346028 1106 58-59 0.6946513944755672 1106 60-61 0.6975148377436433 1106 62-63 0.18294123037866328 1106 64-65 0.30158414873188377 1106 66-67 0.4668463031158474 1106 68-69 -2.3613189142405133 1106 70-71 -2.410416457020837 1106 72-73 0.7897370544993123 1106 74-75 1.066457546483214 1106 76-77 1.0011000549039863 1106 78-79 0.8484140796341109 1106 80-81 0.7246031291463488 1106 82-83 0.38894954544069194 1106 84-85 0.2389381764523968 1106 86-87 0.3865839786370948 1106 88-89 0.44270785442452265 1106 90-91 0.6251468966482712 1106 92-93 0.4782124851086067 1106 94-95 0.37400806041291546 1106 96-97 0.31815466305526385 1106 98-99 0.4757347109006389 1106 100-101 0.7609087713938578 1107 1 -0.024600512756844495 1107 2 0.11394237734080548 1107 3 0.06749626803093278 1107 4 -0.17481693208073779 1107 5 0.2485086664165408 1107 6 0.11559672522000852 1107 7 0.04417431495176061 1107 8 0.10569306578490512 1107 9 0.07150939997845995 1107 10-11 -0.06921556438699383 1107 12-13 0.05952320408727019 1107 14-15 -0.017339894005665712 1107 16-17 -0.2709622249486685 1107 18-19 0.03378804081597053 1107 20-21 0.07952947001393085 1107 22-23 0.22931826533413613 1107 24-25 0.06437933634214232 1107 26-27 0.29270968409227294 1107 28-29 0.09658069062984964 1107 30-31 -0.05304193186283612 1107 32-33 0.039061915207113884 1107 34-35 -0.2277741428116684 1107 36-37 -0.11832249066978306 1107 38-39 0.023600017140473994 1107 40-41 -0.1331834876576039 1107 42-43 -0.2612086858807956 1107 44-45 0.036072912678648095 1107 46-47 0.2784988448223018 1107 48-49 0.09569499765444789 1107 50-51 0.18866713000056734 1107 52-53 -0.20073642828167237 1107 54-55 -0.3700662901348686 1107 56-57 0.13881024030890998 1107 58-59 0.08077855876698692 1107 60-61 0.19939858814795386 1107 62-63 0.14582482896077664 1107 64-65 -0.0619734908950349 1107 66-67 0.02063385435583953 1107 68-69 -2.136635678051384 1107 70-71 -2.758009662647499 1107 72-73 0.7279235548656651 1107 74-75 0.7373140382023067 1107 76-77 0.3744741546973742 1107 78-79 0.2372872453909416 1107 80-81 0.08104039027392318 1107 82-83 0.3125949448980947 1107 84-85 0.10280482375623379 1107 86-87 0.06497382173215627 1107 88-89 0.09885203270338394 1107 90-91 -0.07029241407895626 1107 92-93 0.08245327511210832 1107 94-95 0.48952453642170823 1107 96-97 0.2887552023541069 1107 98-99 0.15809957264342955 1107 100-101 0.614208098755519 1108 1 0.010628729942936843 1108 2 0.011956304260714035 1108 3 -0.011559287200661572 1108 4 -0.027447577135880863 1108 5 -0.23827458829080683 1108 6 -0.16441033903892333 1108 7 0.06124641287838273 1108 8 0.23308506301251697 1108 9 0.0762369553366753 1108 10-11 0.2918613685583651 1108 12-13 0.14454943264770037 1108 14-15 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-0.283467756303434 2316 92-93 -0.3868177172674052 2316 94-95 -0.22425039201108632 2316 96-97 0.05896643282967062 2316 98-99 0.16073151877959901 2316 100-101 0.002225867322675157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 20.0 4 74.0 5 240.0 6 522.0 7 1162.0 8 2243.0 9 3646.0 10 5954.0 11 8761.0 12 11277.0 13 14121.0 14 17909.0 15 22976.0 16 27825.0 17 31047.0 18 25474.0 19 19491.0 20 18257.0 21 18525.0 22 19613.0 23 21336.0 24 24711.0 25 29742.0 26 36610.0 27 44776.0 28 53605.0 29 63701.0 30 74086.0 31 84685.0 32 97410.0 33 111221.0 34 127747.0 35 138795.0 36 129975.0 37 103384.0 38 46270.0 39 3247.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.27618281832183 20.45026878920566 15.461038410956546 17.812509981515966 2 14.051121245618614 24.374038317326118 40.053979213767136 21.520861223288136 3 21.47839241122349 30.64848799987226 31.543212747744963 16.329906841159282 4 11.160160261710764 18.422314344757236 39.818892593246275 30.598632800285724 5 10.538241895409001 43.13148191987359 32.89946398657641 13.430812198140995 6 24.19849644104026 39.15466635750381 19.344801516749293 17.30203568470664 7 23.898381685155115 39.27851662918992 19.929827435750937 16.89327424990402 8 23.510099975771443 33.32405381932995 20.37899293900721 22.7868532658914 9 26.71410095046753 20.02944915092447 22.219113623599306 31.0373362750087 10-11 22.57357586697678 27.8738153920922 26.753289131113966 22.799319609817058 12-13 29.588482480115573 26.025843330794775 26.566716350249127 17.81895783884053 14-15 25.663109211086265 26.44088752881914 30.06295259325391 17.833050666840684 16-17 17.84119905153882 30.567011364941994 32.00233817688156 19.589451406637632 18-19 16.861872139778736 30.921491091660375 35.312590249700094 16.9040465188608 20-21 18.906535392783777 28.90935395838381 32.93889613585764 19.245214512974773 22-23 18.08888291851375 28.057986227177633 31.864794606946255 21.988336247362362 24-25 18.622687185981107 28.45944075218047 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50-51 21.83331226005994 30.05887085232823 30.945129364425444 17.16268752318639 52-53 18.298817616144103 30.201113616377494 33.50688438867101 17.993184378807392 54-55 16.50798424473858 29.950242156228796 34.26717267235877 19.274600926673855 56-57 18.73978567051312 32.720609552644056 29.667395217949093 18.872209558893736 58-59 17.619006287517664 30.320937676847283 31.067871166645254 20.992184868989796 60-61 18.18866347259739 28.47219642658148 32.20423065480272 21.134909446018412 62-63 22.80528803181011 27.73075260904353 30.760766372366444 18.70319298677992 64-65 21.299995521973052 28.3465354130355 32.73823016572518 17.615238899266263 66-67 17.916955351707898 29.629392720235252 33.618513016812855 18.835138911243995 68-69 18.233799903931068 32.13970828325373 31.563294675026203 18.063197137789004 70-71 18.114077627409443 31.494634009699052 29.826712652532432 20.564575710359076 72-73 17.99997631302477 30.4175247389556 28.513649271137837 23.068849676881793 74-75 18.815674913324827 30.580021247268125 30.409501328214727 20.19480251119232 76-77 19.772011621654897 28.83906567681811 31.754982572386677 19.633940129140317 78-79 18.130576953320144 28.51827794755967 33.45816150221507 19.892983596905115 80-81 17.288314097444022 32.71437611535355 31.377635410559453 18.619674376642983 82-83 17.317798141316977 32.11995597143489 29.42127984832291 21.14096603892522 84-85 18.08468282902755 29.264904503502564 29.764506883460705 22.885905784009182 86-87 19.62499288404958 29.530985972205443 30.944560845194406 19.89946029855057 88-89 21.35535246238227 26.98222666683791 31.822329843322667 19.84009102745715 90-91 18.05445679797027 28.747536690596537 33.602022230792436 19.595984280640756 92-93 16.916443029085556 31.56752501148128 31.540414270177802 19.975617689255365 94-95 16.901840469486114 32.18317017065211 29.649823775967256 21.265165583894518 96-97 17.036895570482848 30.153599883328674 27.929865255036223 24.87963929115225 98-99 18.45444840784232 29.4725440674877 28.984195589345692 23.08881193532429 100-101 20.548741284668655 29.744292421324214 29.713881625630606 19.99308466837652 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9667.0 1 6398.0 2 3102.0 3 3038.5 4 2602.0 5 2532.0 6 4539.0 7 8529.5 8 11287.5 9 11808.0 10 10338.0 11 8159.5 12 7130.0 13 6444.5 14 6183.5 15 6087.0 16 5881.5 17 5899.0 18 6232.0 19 6686.5 20 7253.5 21 7861.5 22 8520.0 23 9389.5 24 10481.5 25 11929.5 26 14204.5 27 16608.0 28 18214.5 29 20036.5 30 22699.5 31 25514.5 32 29111.0 33 33836.5 34 38583.0 35 41944.5 36 45547.5 37 48841.0 38 47939.5 39 44902.0 40 41820.0 41 38594.0 42 35993.0 43 35464.5 44 38069.0 45 40384.0 46 46593.5 47 68964.0 48 125233.0 49 117925.5 50 55986.5 51 33500.5 52 21792.0 53 15376.5 54 11883.5 55 10028.0 56 8722.5 57 7806.5 58 7452.5 59 7398.5 60 7434.0 61 7195.0 62 6017.5 63 4208.0 64 2820.0 65 2145.0 66 1716.5 67 1487.5 68 1320.0 69 1150.5 70 1045.5 71 903.0 72 717.5 73 617.5 74 500.5 75 353.5 76 246.5 77 166.0 78 106.5 79 67.5 80 46.5 81 26.5 82 15.0 83 10.5 84 7.0 85 5.0 86 2.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01992434303470654 2 0.01839704147803914 3 4.1653678818201825E-4 4 0.0904579058335283 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.006872857005003301 12-13 0.0 14-15 0.0 16-17 7.289393793185319E-4 18-19 6.942279803033637E-5 20-21 0.0 22-23 2.429797931061773E-4 24-25 0.003471139901516819 26-27 0.0031240259113651373 28-29 0.0011107647684853819 30-31 1.0413419704550456E-4 32-33 5.553823842426909E-4 34-35 0.0032281601084106415 36-37 0.08323793483837331 38-39 0.09444971672027264 40-41 0.07518489026685429 42-43 0.07966266073981099 44-45 0.0778923793900374 46-47 0.07695517161662786 48-49 0.0714707705722313 50-51 0.07129721357715546 52-53 0.05657958039472415 54-55 0.04692981146850739 56-57 0.026137683458421646 58-59 0.020410302620918894 60-61 0.012183701054324033 62-63 0.001839704147803914 64-65 0.005727380837502751 66-67 0.15095987431696645 68-69 0.7099175326582198 70-71 0.865979982630416 72-73 0.3513487808315324 74-75 0.05099104515328207 76-77 0.1935507609085778 78-79 0.014058116601143117 80-81 0.002950468916289296 82-83 0.0014231673596218958 84-85 2.429797931061773E-4 86-87 0.0015967243546977366 88-89 3.124025911365137E-4 90-91 0.004130656482805015 92-93 0.00420007928083535 94-95 0.07553200425700597 96-97 0.03516264720236537 98-99 0.012947351832657734 100-101 0.01187129846318752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1440449.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.85321293681169 #Duplication Level Percentage of deduplicated Percentage of total 1 77.06639498330622 29.172106595755267 2 11.990616611555357 9.077667276817527 3 4.228931058693628 4.802358835794792 4 2.031037996300644 3.0752525502689454 5 1.149373702842164 2.175374375882808 6 0.7063688770700954 1.6042998909402528 7 0.47913529788291864 1.2695767319413374 8 0.3345862235770307 1.013213085342802 9 0.24787629221685323 0.8444622664144712 >10 1.4842544635276618 10.851875705255312 >50 0.15510573275312017 4.080063709322345 >100 0.10770930091211765 7.688438627364922 >500 0.00845884516470239 2.115418113426857 >1k 0.006579101794768526 5.222798686298174 >5k 0.0015037946959470913 3.628301393873605 >10k+ 0.002067717706927251 13.378792155300529 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 32576 2.2615170686362376 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 28368 1.9693859345245823 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19793 1.3740854414144479 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 18162 1.2608568578269692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17163 1.191503482594663 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 13120 0.9108271101580132 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12884 0.8944433298228539 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 12471 0.8657717142363248 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11577 0.8037077327972042 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 11459 0.7955158426296245 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 10452 0.7256070850130758 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 8232 0.571488473385729 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 8212 0.5701000174251223 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 7197 0.49963587742433085 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5702 0.395848794368978 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5598 0.388628823373823 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5553 0.38550479746245786 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5336 0.37044005028987487 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 5162 0.35836048343259636 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4545 0.3155266170478788 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4337 0.30108667505756886 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4252 0.29518573722499025 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4145 0.2877574978357443 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 3817 0.2649868200817939 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 2904 0.20160380548009685 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2806 0.19480037127312386 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2602 0.18063812047493524 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2377 0.16501799091810956 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 2296 0.15939474427765232 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 2175 0.15099458571598162 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2138 0.14842594218885918 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2114 0.1467597950361311 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 2087 0.14488537948931202 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2037 0.1414142395877952 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1851 0.12850159915415263 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1846 0.12815448516400096 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1840 0.12773794837581892 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1735 0.12044855458263361 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1620 0.11246493280914494 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1587 0.11017398047414383 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1528 0.10607803539035399 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1471 0.1021209359026248 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1452 0.10080190274004842 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.942279803033638E-5 0.0 16-17 0.0 0.0 0.0 2.0826839409100913E-4 3.471139901516819E-5 18-19 0.0 0.0 0.0 3.8182538916685003E-4 6.942279803033638E-5 20-21 0.0 0.0 0.0 6.942279803033638E-4 6.942279803033638E-5 22-23 0.0 0.0 0.0 0.0014578787586370639 6.942279803033638E-5 24-25 0.0 0.0 0.0 0.0026727777241679503 6.942279803033638E-5 26-27 0.0 0.0 0.0 0.004547193270987033 6.942279803033638E-5 28-29 0.0 0.0 0.0 0.008330735763640365 6.942279803033638E-5 30-31 0.0 0.0 0.0 0.02047972541894923 6.942279803033638E-5 32-33 0.0 0.0 0.0 0.040577625448731613 6.942279803033638E-5 34-35 0.0 0.0 0.0 0.08066929131125086 6.942279803033638E-5 36-37 0.0 0.0 0.0 0.13894973025771826 6.942279803033638E-5 38-39 0.0 0.0 0.0 0.2099345412437372 6.942279803033638E-5 40-41 0.0 0.0 0.0 0.28990960457468473 6.942279803033638E-5 42-43 0.0 0.0 0.0 0.3639143072750233 6.942279803033638E-5 44-45 0.0 0.0 0.0 0.44642330273407804 6.942279803033638E-5 46-47 0.0 0.0 0.0 0.5585758329520865 6.942279803033638E-5 48-49 0.0 0.0 0.0 0.6912080885890441 6.942279803033638E-5 50-51 0.0 0.0 0.0 0.8251593773885781 6.942279803033638E-5 52-53 0.0 0.0 0.0 0.9428657314490134 6.942279803033638E-5 54-55 0.0 0.0 0.0 1.0608844881005854 6.942279803033638E-5 56-57 0.0 0.0 0.0 1.2011532515208798 6.942279803033638E-5 58-59 0.0 0.0 0.0 1.370996126902098 6.942279803033638E-5 60-61 0.0 0.0 0.0 1.598251656254404 6.942279803033638E-5 62-63 0.0 0.0 0.0 1.7854155197441908 6.942279803033638E-5 64-65 0.0 0.0 0.0 1.9358547230759298 6.942279803033638E-5 66-67 0.0 0.0 0.0 2.0953188901516127 6.942279803033638E-5 68-69 0.0 0.0 0.0 2.2781091173654877 6.942279803033638E-5 70-71 0.0 0.0 0.0 2.516229314609542 6.942279803033638E-5 72-73 0.0 0.0 0.0 2.8075273751448333 6.942279803033638E-5 74-75 0.0 0.0 0.0 3.0940005512170163 6.942279803033638E-5 76-77 0.0 0.0 0.0 3.37550999723003 6.942279803033638E-5 78-79 0.0 0.0 0.0 3.7012417655883683 6.942279803033638E-5 80-81 0.0 0.0 0.0 4.06796769618362 6.942279803033638E-5 82-83 0.0 0.0 0.0 4.446044254256832 6.942279803033638E-5 84-85 0.0 0.0 0.0 4.936585745139189 6.942279803033638E-5 86-87 0.0 0.0 0.0 5.464546124159897 6.942279803033638E-5 88-89 0.0 0.0 0.0 5.885491260016842 6.942279803033638E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 265 0.0 37.571568 9 CGTCACT 110 7.0675014E-8 34.51601 1 TAGCGAC 55 2.516781E-6 30.551865 66-67 GTAGCGA 55 2.5195422E-6 30.54757 64-65 GACGCAT 35 0.0080635715 27.366985 70-71 GCTTATG 325 0.0 26.28527 1 GGTAGCG 85 1.01505066E-7 25.413523 64-65 ATGGGCG 245 1.0913936E-11 25.157248 5 CATGGGT 1550 0.0 24.79884 4 GGAACGC 330 0.0 24.424227 8 AGCGACG 70 1.639382E-5 24.005037 66-67 TCGAAAC 50 0.0015557931 24.001661 64-65 TAGCAAC 635 0.0 23.81602 66-67 GTACATG 16775 0.0 23.651955 1 TACATGG 16660 0.0 23.527897 2 GTGTAGC 415 0.0 22.872057 1 GCAACGC 390 0.0 22.775608 68-69 CATGGGG 4510 0.0 22.622467 4 TAGGCAT 465 0.0 22.431358 5 ACATGGG 16625 0.0 22.37702 3 >>END_MODULE