FastQCFastQC Report
Fri 27 May 2016
SRR939467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335575
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158530.6787621891825354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128580.5505282425098744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85990.368174860580371No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69600.2979994219838798No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA39370.16856662706185843No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.14184943750468298No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31820.13624054033803237No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27240.11663080825920812No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.11423311176048724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA249500.027.4708141
GTACATG158750.025.6961571
TACATGG157200.025.3031652
GGTATCA178800.024.9474181
GAGTACT140400.023.8069912-13
ACATGGG162300.023.3642163
TATCAAC295350.023.196052
ATACCGT1502.5423567E-522.6807676
ATCAACG304350.022.3355273
TCAACGC307250.022.1687284
TACCTGG14050.021.9112532
AGTACTT148350.021.9012812-13
CAACGCA309250.021.8317345
GTACTTT154600.021.76556414-15
AACGCAG313600.021.4336996
CATGGGA118350.021.2482974
ATGGGAT47550.021.1555985
ACTTTTT179250.020.31358316-17
ACGCAGA334450.019.7605427
CGCAGAG334950.019.6400668
Produced by FastQC (version 0.11.5)