FastQCFastQC Report
Fri 27 May 2016
SRR939467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335575
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215920.924483264292519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197690.8464296800573734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140450.6013508450809757No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA105680.45247958211575307No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA52460.2246127827194588No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51670.22123031801590615No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48210.20641597893452363No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA47050.20144932190145895No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.18834762317630563No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29870.12789141860141506No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25570.10948053477195124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203250.025.8367981
GTATCAA272700.024.0711
GAGTACT161350.023.00453812-13
GTACATG177900.022.724931
TACATGG178800.022.551252
GTACTTT172000.021.73161714-15
ACATGGG179950.021.4292373
AGTACTT166400.020.79746412-13
TATCAAC325800.020.06952
CATGGGA130950.019.933354
ATCAACG327850.019.9116943
TCAACGC331800.019.674654
ATGGGAG38500.019.5645835
ACTTTTT197600.019.47959316-17
CAACGCA335500.019.401195
AACGCAG341800.019.0574516
TACTTTT187600.018.49772314-15
CATTCCG2854.4105036E-818.2844529
GTCGGGA4750.017.9550862
TATCACG1600.00121164717.7680532
Produced by FastQC (version 0.11.5)