##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939467_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2335575 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.959117562056452 31.0 31.0 31.0 30.0 34.0 2 31.197658820633034 31.0 31.0 34.0 30.0 34.0 3 31.172393521937853 31.0 31.0 34.0 28.0 34.0 4 35.1183370262141 35.0 35.0 37.0 32.0 37.0 5 34.74630058979052 35.0 35.0 37.0 32.0 37.0 6 34.73050876122582 35.0 35.0 37.0 32.0 37.0 7 34.593489825845886 35.0 35.0 37.0 30.0 37.0 8 34.619878616614756 35.0 35.0 37.0 30.0 37.0 9 35.930499341703865 37.0 35.0 39.0 30.0 39.0 10-11 35.79226164863042 37.0 35.0 39.0 30.0 39.0 12-13 35.75586953962086 37.0 35.0 39.0 30.0 39.0 14-15 36.53767958639736 38.0 35.0 40.0 30.0 41.0 16-17 36.57131241771299 38.0 35.0 40.0 30.5 41.0 18-19 36.58867002772337 38.0 35.0 40.0 30.0 41.0 20-21 36.53455829934812 38.0 35.0 40.0 30.0 41.0 22-23 36.39488134613532 38.0 35.0 40.0 30.0 41.0 24-25 36.166826584459926 38.0 34.0 40.0 29.5 41.0 26-27 35.770725196150835 38.0 34.0 40.0 28.0 41.0 28-29 35.47328238228273 38.0 34.0 40.0 27.0 41.0 30-31 35.22066600301853 37.5 34.0 40.0 27.0 41.0 32-33 34.965908395148944 37.0 33.0 40.0 27.0 41.0 34-35 34.86380291791101 37.0 33.0 40.0 26.0 41.0 36-37 34.80863363411579 37.5 33.0 40.0 25.0 41.0 38-39 34.74199030217399 37.0 33.0 40.0 25.0 41.0 40-41 34.49655331128309 37.0 33.0 40.0 24.5 41.0 42-43 34.16995600655085 37.0 33.0 40.0 24.0 41.0 44-45 33.93535553343395 37.0 33.0 40.0 24.0 41.0 46-47 33.542247198227415 37.0 32.5 40.0 22.5 41.0 48-49 33.173759780782035 37.0 32.0 40.0 20.5 41.0 50-51 32.90580970424842 37.0 31.0 40.0 19.0 41.0 52-53 32.52930563137557 36.0 31.0 39.0 17.5 41.0 54-55 32.323004613424956 36.0 31.0 39.0 16.0 40.0 56-57 31.957601019021013 36.0 30.0 39.0 13.5 40.0 58-59 31.552076469391906 35.0 30.0 39.0 11.0 40.0 60-61 31.287356218490093 35.0 29.0 39.0 8.5 40.0 62-63 30.903628870834805 35.0 29.0 38.0 8.0 40.0 64-65 30.47863288483564 34.5 28.0 38.0 7.5 40.0 66-67 28.83840553182835 33.5 25.0 37.5 4.0 39.5 68-69 25.271710606595803 30.0 15.5 35.0 2.0 39.0 70-71 24.679869197092792 29.5 13.0 35.0 2.0 38.0 72-73 26.45566038341736 31.0 21.0 35.0 2.0 38.0 74-75 27.042699977521593 31.5 23.0 35.0 2.0 38.0 76-77 27.069320616978686 31.5 25.0 34.5 2.0 36.5 78-79 27.884992988878544 32.5 26.0 35.0 2.0 37.0 80-81 27.741751817004378 33.0 26.0 35.0 2.0 37.0 82-83 27.33560258180534 33.0 26.0 35.0 2.0 36.0 84-85 26.99119360328827 32.0 25.5 35.0 2.0 36.0 86-87 26.53548826306156 32.0 25.0 35.0 2.0 35.5 88-89 26.032806268263705 32.0 23.5 34.0 2.0 35.0 90-91 25.56726052471019 31.5 22.0 34.0 2.0 35.0 92-93 25.026404204532074 31.0 19.5 34.0 2.0 35.0 94-95 23.693974288986652 31.0 7.0 34.0 2.0 35.0 96-97 22.88310437472571 30.0 2.0 34.0 2.0 35.0 98-99 22.545212420924184 30.0 2.0 34.0 2.0 35.0 100-101 21.328088158162338 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4511316119063373 1101 2 0.3097284252987649 1101 3 0.4243804167134222 1101 4 0.29715610212377186 1101 5 0.3742752885513383 1101 6 0.5243427227324844 1101 7 0.5561653147445327 1101 8 0.5846999442492233 1101 9 0.7525673205482519 1101 10-11 0.8342467087150212 1101 12-13 0.844383453849467 1101 14-15 0.9029539675894966 1101 16-17 0.7478054111821066 1101 18-19 0.9425078577974872 1101 20-21 0.8902239622160479 1101 22-23 0.9821342188220115 1101 24-25 0.8151006481102598 1101 26-27 0.9520616890233029 1101 28-29 0.8294887129338022 1101 30-31 0.9890635050588656 1101 32-33 0.9379298646813368 1101 34-35 1.083002775528847 1101 36-37 0.9304835705756602 1101 38-39 1.023114422464026 1101 40-41 1.1042181582205544 1101 42-43 1.313248487568977 1101 44-45 1.1272812147002256 1101 46-47 1.035677080120486 1101 48-49 0.8596106862074038 1101 50-51 0.8515965836369261 1101 52-53 0.9945082892770003 1101 54-55 0.8232946197235478 1101 56-57 0.7632743400296178 1101 58-59 0.6329051181518714 1101 60-61 0.5063913909329969 1101 62-63 0.5871747093959847 1101 64-65 0.5727332441673489 1101 66-67 -2.5081928946128365 1101 68-69 -7.813057417220026 1101 70-71 -10.932814399384075 1101 72-73 -10.706842525496292 1101 74-75 -9.007652399365558 1101 76-77 -4.01439312354476 1101 78-79 -1.0864644951001843 1101 80-81 -0.3678119142195726 1101 82-83 -0.2485277304279201 1101 84-85 -0.3385496603943352 1101 86-87 -0.29736206552065525 1101 88-89 -0.17216753883085545 1101 90-91 -0.38987159811722805 1101 92-93 -0.8657643842548026 1101 94-95 -4.302638758482146 1101 96-97 -1.1613523202382616 1101 98-99 -0.4343878595963666 1101 100-101 -0.16098321038549201 1102 1 0.36973117611596606 1102 2 0.23559927582993723 1102 3 0.40884524290030555 1102 4 0.2395915627640619 1102 5 0.3748513444862098 1102 6 0.42039951438327705 1102 7 0.4106135050850952 1102 8 0.49838729289255923 1102 9 0.74301679995596 1102 10-11 0.6452232030365721 1102 12-13 0.5923004842697637 1102 14-15 0.5520889262984525 1102 16-17 0.6282159556993889 1102 18-19 0.7006571020445591 1102 20-21 0.8255745027999168 1102 22-23 0.8084286083942018 1102 24-25 0.6881371898533786 1102 26-27 0.8093320666368982 1102 28-29 0.7455344832552768 1102 30-31 0.6800779736367275 1102 32-33 0.4947815551053054 1102 34-35 0.796638149322618 1102 36-37 1.0082420347034429 1102 38-39 0.9748751739395445 1102 40-41 0.8627448408069185 1102 42-43 1.1394026764854743 1102 44-45 0.953611222378818 1102 46-47 0.8854516718672159 1102 48-49 0.6774851663777781 1102 50-51 0.6649677668201122 1102 52-53 0.8165802699812374 1102 54-55 0.7224304709135296 1102 56-57 0.6169025025766999 1102 58-59 0.5625485671139607 1102 60-61 0.5552315225918001 1102 62-63 0.5885650325649863 1102 64-65 0.6183635272493468 1102 66-67 -2.3612333178370726 1102 68-69 -7.488537036523315 1102 70-71 -10.956236428022656 1102 72-73 -8.942374840124739 1102 74-75 -7.364680346678671 1102 76-77 -3.6079688666244607 1102 78-79 -0.8454329557943474 1102 80-81 -0.16121602508454203 1102 82-83 -0.05627092826207658 1102 84-85 -0.17507236933063552 1102 86-87 -0.02019226565059995 1102 88-89 0.04287300577080444 1102 90-91 -0.06093416655429351 1102 92-93 -0.35696622516729803 1102 94-95 -3.982406343441472 1102 96-97 -0.817541286706291 1102 98-99 -0.11858888461894068 1102 100-101 0.07186500812501961 1103 1 0.39668579580289176 1103 2 0.2545795287689785 1103 3 0.40923199984353076 1103 4 0.20078611772534316 1103 5 0.3794026003858093 1103 6 0.38488602592298093 1103 7 0.41875411493841597 1103 8 0.5138106295569926 1103 9 0.6571654917817895 1103 10-11 0.7301314792608764 1103 12-13 0.6097634959585534 1103 14-15 0.7367505514012223 1103 16-17 0.6697158334342888 1103 18-19 0.613959763057089 1103 20-21 0.5701241929576994 1103 22-23 0.6241031343362309 1103 24-25 0.7191011378682646 1103 26-27 0.7121045680934657 1103 28-29 0.8099596127252795 1103 30-31 0.9206460166590631 1103 32-33 0.8542456222662764 1103 34-35 0.7875729876394999 1103 36-37 0.7112439703595683 1103 38-39 0.7838790003380751 1103 40-41 0.8941877124397948 1103 42-43 0.8646396274933537 1103 44-45 0.8145543229468473 1103 46-47 0.8534571257639101 1103 48-49 0.7893998378152816 1103 50-51 0.6037152918875819 1103 52-53 0.755638292255945 1103 54-55 0.8061770727547426 1103 56-57 0.7812603238625542 1103 58-59 0.904986829477398 1103 60-61 0.6601589931889151 1103 62-63 0.6143146236630912 1103 64-65 0.5708584682190114 1103 66-67 -2.761572603484524 1103 68-69 -7.512605716447016 1103 70-71 -11.267141133607051 1103 72-73 -8.951376296077488 1103 74-75 -7.288199161156353 1103 76-77 -3.646226068101125 1103 78-79 -0.8413847958216962 1103 80-81 -0.2344330131961314 1103 82-83 0.09317819261443461 1103 84-85 -0.04237419070631532 1103 86-87 0.0069710936204003815 1103 88-89 0.176542365388638 1103 90-91 0.1456273365329004 1103 92-93 0.12793660838872967 1103 94-95 0.9726866528893261 1103 96-97 1.1151558314554428 1103 98-99 0.8863099067032323 1103 100-101 0.9366266317306682 1104 1 0.35687406139563294 1104 2 0.23646338848721626 1104 3 0.3832301175523867 1104 4 0.22883079660500982 1104 5 0.4787627673114798 1104 6 0.5462095027627782 1104 7 0.5455643610224499 1104 8 0.4188525068870561 1104 9 0.6373868433835597 1104 10-11 0.6160750324137112 1104 12-13 0.7328675253748287 1104 14-15 0.7116925505695093 1104 16-17 0.8268210265150415 1104 18-19 0.9031094124986012 1104 20-21 0.7904717237273289 1104 22-23 0.6075368747697425 1104 24-25 0.7226942711534434 1104 26-27 0.9460088813125509 1104 28-29 1.138082902795098 1104 30-31 1.0917315988925722 1104 32-33 0.9330793313282584 1104 34-35 0.9504563767849561 1104 36-37 1.0152545090012524 1104 38-39 0.968494918100923 1104 40-41 1.0787042262615003 1104 42-43 1.099026824995299 1104 44-45 1.0797567422016598 1104 46-47 1.0645122119626365 1104 48-49 1.1378060916911181 1104 50-51 1.1077926773997717 1104 52-53 1.241938787634112 1104 54-55 1.1733022742985142 1104 56-57 1.14395612226771 1104 58-59 1.1399517553895429 1104 60-61 1.0850497400499393 1104 62-63 1.1119106898205189 1104 64-65 0.8054750243557898 1104 66-67 -2.0740821790935335 1104 68-69 -6.794823080947584 1104 70-71 -8.262427198086467 1104 72-73 -7.693015421468722 1104 74-75 -6.4622727893819665 1104 76-77 -2.9712682919770366 1104 78-79 -0.21084279811254447 1104 80-81 0.6259062734301182 1104 82-83 0.8075186246659136 1104 84-85 0.7686142310144461 1104 86-87 1.0298572551326508 1104 88-89 0.9607551044140763 1104 90-91 0.7550587642288455 1104 92-93 0.6537729694384353 1104 94-95 1.635297821150104 1104 96-97 1.890723619471519 1104 98-99 1.6382346041139897 1104 100-101 1.323951352487672 1105 1 0.43477257817939474 1105 2 0.3534095243186002 1105 3 0.4392738333993833 1105 4 0.36170823610540026 1105 5 0.39146846598438856 1105 6 0.5254757010453659 1105 7 0.5818266561044112 1105 8 0.5626432953335794 1105 9 0.6748388340158797 1105 10-11 0.8015676163325267 1105 12-13 0.672247697115651 1105 14-15 0.8543264303509375 1105 16-17 0.8900841022371466 1105 18-19 0.776773737244902 1105 20-21 0.7347761733760336 1105 22-23 0.9338959692272013 1105 24-25 0.835023701286147 1105 26-27 0.9538199664023281 1105 28-29 1.0307433243407687 1105 30-31 0.7844357362852321 1105 32-33 0.8872042337482497 1105 34-35 1.1384610606257084 1105 36-37 1.0717915894071837 1105 38-39 0.9482154489362031 1105 40-41 0.9698267867611108 1105 42-43 1.1498777227314392 1105 44-45 1.0278832059018939 1105 46-47 1.1506496046246184 1105 48-49 0.8585008614022769 1105 50-51 0.9916240591327963 1105 52-53 1.2386600991095307 1105 54-55 1.3024779183266162 1105 56-57 1.1986305953356293 1105 58-59 1.2654396554676062 1105 60-61 1.4378100835315948 1105 62-63 1.355937231584761 1105 64-65 1.3098747120997416 1105 66-67 2.0118257053717308 1105 68-69 -4.747029169940564 1105 70-71 -5.088658267563435 1105 72-73 -1.4768983254499837 1105 74-75 1.32952580546581 1105 76-77 1.4496606697715926 1105 78-79 1.3309917061809777 1105 80-81 1.3444058831100527 1105 82-83 1.5574327542521758 1105 84-85 1.414236401193996 1105 86-87 1.6046753659835495 1105 88-89 1.6337980396209453 1105 90-91 1.5021219960789587 1105 92-93 1.6079056782596695 1105 94-95 2.472098757918257 1105 96-97 2.3682817771608278 1105 98-99 1.8411307789773765 1105 100-101 1.5150661065860298 1106 1 0.20141272656423936 1106 2 0.14051208983381258 1106 3 0.18358735147919347 1106 4 0.06823298221672758 1106 5 0.2732180849218224 1106 6 0.38322972745346107 1106 7 0.23459186646931585 1106 8 0.09730900704827405 1106 9 0.49215857764799864 1106 10-11 0.5135429344968188 1106 12-13 0.330787208253696 1106 14-15 0.34765587041852086 1106 16-17 0.2814854942632863 1106 18-19 0.3311448095457834 1106 20-21 0.510106237835295 1106 22-23 0.4499206510629108 1106 24-25 0.5657626504716831 1106 26-27 0.5966288474557118 1106 28-29 0.6586678831345907 1106 30-31 0.43054243380419166 1106 32-33 0.572281419578232 1106 34-35 0.6310823670734678 1106 36-37 0.55875811099461 1106 38-39 0.4937292681931851 1106 40-41 0.47644232676809395 1106 42-43 0.4038156141240208 1106 44-45 0.5095205812531205 1106 46-47 0.38424011387112245 1106 48-49 0.20809629232436322 1106 50-51 0.11694600650025677 1106 52-53 0.439495682089337 1106 54-55 0.3440438191952566 1106 56-57 0.3192365490939153 1106 58-59 0.5248284862023702 1106 60-61 0.3984446123913443 1106 62-63 0.46921062331708185 1106 64-65 0.21112775600916578 1106 66-67 1.0851453755460092 1106 68-69 -5.308882731727792 1106 70-71 -6.224682435194236 1106 72-73 -2.544030494476484 1106 74-75 0.11351801893430391 1106 76-77 0.18106242908696757 1106 78-79 0.1334374851095923 1106 80-81 0.1951060465537573 1106 82-83 0.27196051213512007 1106 84-85 0.3190277625514959 1106 86-87 0.35792503083213845 1106 88-89 0.17838540854460305 1106 90-91 0.19128982888890533 1106 92-93 0.4326467944516139 1106 94-95 1.2848254029148585 1106 96-97 1.4227071348087073 1106 98-99 1.1294146200880597 1106 100-101 1.1651169026854191 1107 1 0.005195685127091565 1107 2 -0.010999531106850924 1107 3 0.007351897411872699 1107 4 -0.10177029629116419 1107 5 0.10600710767057109 1107 6 0.1754054434341228 1107 7 0.0506385218343155 1107 8 -0.07802493261333154 1107 9 3.84501463187803E-4 1107 10-11 0.04542710111004311 1107 12-13 0.16475667447708986 1107 14-15 0.15041230076467116 1107 16-17 -0.003975226412357813 1107 18-19 0.23800402608174664 1107 20-21 0.22040927251733677 1107 22-23 0.05094046571431221 1107 24-25 0.20278794796843158 1107 26-27 0.17942027865837673 1107 28-29 0.14315706360696367 1107 30-31 0.2582031055280112 1107 32-33 0.22074521735480346 1107 34-35 0.368684323701423 1107 36-37 0.30584779549617735 1107 38-39 0.21146915697679702 1107 40-41 0.10368471025838488 1107 42-43 0.1632344752685242 1107 44-45 0.07253582104631562 1107 46-47 -0.09122549295540239 1107 48-49 -0.07843122290685045 1107 50-51 -0.14181700253777763 1107 52-53 0.060635118625533835 1107 54-55 0.06420781684339971 1107 56-57 0.07408883108895026 1107 58-59 0.0821843861467606 1107 60-61 0.03372265183760703 1107 62-63 0.2352518295550965 1107 64-65 0.11246175347366716 1107 66-67 0.9526547451843754 1107 68-69 -5.5457988811037175 1107 70-71 -6.2600852777117595 1107 72-73 -2.599533766511655 1107 74-75 -0.0982993311462117 1107 76-77 -9.32616723329005E-4 1107 78-79 0.026666959889016795 1107 80-81 0.07809206577984185 1107 82-83 0.27121885885795294 1107 84-85 0.14507793124863966 1107 86-87 0.18715624030049227 1107 88-89 0.017156353438277705 1107 90-91 0.10107749959184176 1107 92-93 0.3526410412573284 1107 94-95 1.0020035198230168 1107 96-97 1.116328615568321 1107 98-99 0.9780082184777648 1107 100-101 0.8502979567031268 1108 1 0.0028537647523840803 1108 2 0.008126151953259608 1108 3 0.06629022684200336 1108 4 -0.07327693173222372 1108 5 0.075952462861828 1108 6 0.20311816786816195 1108 7 0.15056045782182537 1108 8 0.12220925942413885 1108 9 0.20374125400026344 1108 10-11 0.2538580144589915 1108 12-13 0.12767626854422076 1108 14-15 0.21266835072563595 1108 16-17 0.14337059716297773 1108 18-19 0.18921401827534368 1108 20-21 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0.2435228259120663 2311 58-59 0.5204371492019426 2311 60-61 0.5187223642333478 2311 62-63 0.5125487604068191 2311 64-65 0.5231679451824469 2311 66-67 1.5459109184043136 2311 68-69 3.932279100736128 2311 70-71 4.293517171033056 2311 72-73 2.475161824925955 2311 74-75 1.4016962315309733 2311 76-77 0.7300315970898943 2311 78-79 0.4814979718451369 2311 80-81 0.18114773774664528 2311 82-83 0.2859540575103203 2311 84-85 0.16918376961504578 2311 86-87 0.4710713559584896 2311 88-89 0.6559133278414997 2311 90-91 0.5034252542243216 2311 92-93 0.5084771068311014 2311 94-95 1.1470862265899306 2311 96-97 1.2964822784139578 2311 98-99 1.159446568451063 2311 100-101 1.0213217456837747 2312 1 -0.21191325129258232 2312 2 -0.07314174665708251 2312 3 -0.09745665692759076 2312 4 0.04575588133171493 2312 5 -0.06589701478736032 2312 6 0.07031300045110811 2312 7 0.13508176749476775 2312 8 0.14082638913184553 2312 9 -1.4223653859898846 2312 10-11 -1.1758837305613739 2312 12-13 -1.92583298562473 2312 14-15 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0.9800850370146037 2312 82-83 1.1617140181596106 2312 84-85 0.912912596905926 2312 86-87 1.08413116325573 2312 88-89 1.1628100032849176 2312 90-91 1.0671616044456727 2312 92-93 1.4591511035325837 2312 94-95 2.3852988940474695 2312 96-97 2.586339632078495 2312 98-99 2.3948196189993673 2312 100-101 2.3407062542338757 2313 1 -0.2864528928114396 2313 2 -0.20239671041500884 2313 3 -0.025123564361145156 2313 4 0.020866522883800087 2313 5 -0.18129759849117022 2313 6 0.008564530279450366 2313 7 -3.4213658757585073 2313 8 -1.1415426341931294 2313 9 -0.8069504232817621 2313 10-11 -0.1143206518238955 2313 12-13 -1.3874752466072948 2313 14-15 -0.274715529551365 2313 16-17 -0.008454193731324722 2313 18-19 0.34301340446780415 2313 20-21 0.1516540664326982 2313 22-23 -1.358731760584888 2313 24-25 -2.427533458500065 2313 26-27 -1.9090696802638547 2313 28-29 -0.32259755451966754 2313 30-31 -0.1257873888205694 2313 32-33 -2.397429880740617 2313 34-35 -2.4746550002221994 2313 36-37 -0.9382714350915933 2313 38-39 -0.5136406539925176 2313 40-41 -0.468513003802137 2313 42-43 -0.6322708177411229 2313 44-45 -0.015713979036071635 2313 46-47 -0.1653209871672061 2313 48-49 -0.2871215236614404 2313 50-51 -0.1402062632450125 2313 52-53 0.08374220508548547 2313 54-55 0.20686989878563367 2313 56-57 0.6291742118680759 2313 58-59 0.3258522410198559 2313 60-61 0.6676065961437487 2313 62-63 0.35515868796823113 2313 64-65 -0.06787672082487717 2313 66-67 1.3443127788196954 2313 68-69 4.371854092048846 2313 70-71 4.193810667598235 2313 72-73 2.446534308954714 2313 74-75 1.5216446958863763 2313 76-77 0.9372216748744968 2313 78-79 0.7761207298574853 2313 80-81 0.7122287566982877 2313 82-83 0.5013356297751628 2313 84-85 0.7493262945408716 2313 86-87 0.8566387024608559 2313 88-89 0.6789267730848749 2313 90-91 0.8722336795537728 2313 92-93 1.0713902607163206 2313 94-95 1.4852800198592924 2313 96-97 1.6793846333363085 2313 98-99 1.2091653142744825 2313 100-101 1.0884155950863565 2314 1 -0.3226969130391453 2314 2 -0.1987657427464029 2314 3 -0.07858530112895323 2314 4 -0.10925464638599891 2314 5 -1.3751091186616407 2314 6 -2.7842327907163558 2314 7 -0.8352321887262946 2314 8 -0.2738528118545389 2314 9 0.07005737466407425 2314 10-11 0.22645843653893394 2314 12-13 0.008628649496607466 2314 14-15 -2.6784115743566517 2314 16-17 -2.7175240817208532 2314 18-19 -0.4699059658150375 2314 20-21 -0.10278167561907736 2314 22-23 -0.13290250986025143 2314 24-25 -2.0380076222698875 2314 26-27 -1.3019193057323832 2314 28-29 -0.16365899324026145 2314 30-31 -0.026705310247450598 2314 32-33 0.019021724242549 2314 34-35 -0.025991591918206325 2314 36-37 0.2103047163309526 2314 38-39 -1.2287673502671268 2314 40-41 -4.083391391765048 2314 42-43 -5.19379781592508 2314 44-45 -2.214810982039438 2314 46-47 -3.0708850685602442 2314 48-49 -0.9955060189163873 2314 50-51 -1.0978570199908688 2314 52-53 -2.3900561782759553 2314 54-55 -1.5082937637302365 2314 56-57 -4.093512080746208 2314 58-59 -5.091250549131342 2314 60-61 -2.669919049186131 2314 62-63 -1.6979255241639244 2314 64-65 -0.7031595084325772 2314 66-67 -0.882178277970656 2314 68-69 1.2790631711783966 2314 70-71 1.284692447627581 2314 72-73 -0.9573741538041425 2314 74-75 -0.43155549093107837 2314 76-77 -0.3400695493122363 2314 78-79 0.04469440399013891 2314 80-81 0.2184989484730835 2314 82-83 0.039033333647431334 2314 84-85 -0.14617207246162423 2314 86-87 -0.5184526441558681 2314 88-89 -0.29976053361823674 2314 90-91 0.16225163576753587 2314 92-93 0.285426590807198 2314 94-95 0.39907033133849623 2314 96-97 0.6727691934201054 2314 98-99 0.17178803047113078 2314 100-101 0.12411119077940214 2315 1 -0.28213737168004016 2315 2 -0.21849158572980443 2315 3 -0.17897663757415572 2315 4 0.04879608353896003 2315 5 -1.0250717768930429 2315 6 -0.31693568347191814 2315 7 0.003483439572519842 2315 8 0.002905069672046068 2315 9 0.2681991794930809 2315 10-11 -1.8588998212815966 2315 12-13 -1.3231251110459539 2315 14-15 -0.14387465361278373 2315 16-17 -1.5617467530508051 2315 18-19 -2.788931621709395 2315 20-21 -1.887463979654136 2315 22-23 -1.488044383785585 2315 24-25 -0.8926165618793789 2315 26-27 -0.389930663977637 2315 28-29 -0.271245604158203 2315 30-31 -2.945435637218516 2315 32-33 -2.4263827586871898 2315 34-35 -1.4474442809387966 2315 36-37 -4.757930811543456 2315 38-39 -2.385776841589255 2315 40-41 -1.3870599767087342 2315 42-43 -1.3563484025330226 2315 44-45 -1.2890718414852884 2315 46-47 -1.1917958638853676 2315 48-49 -4.159265129960787 2315 50-51 -3.695960904493557 2315 52-53 -2.142999466725332 2315 54-55 -3.6995889164413747 2315 56-57 -5.948792643223335 2315 58-59 -6.6998387261223975 2315 60-61 -4.987352018314624 2315 62-63 -6.0646732660531235 2315 64-65 -5.582261276075197 2315 66-67 -3.885108590196772 2315 68-69 -1.7803396646801524 2315 70-71 0.4942526677175536 2315 72-73 -1.2034539293105162 2315 74-75 -2.696079852013252 2315 76-77 -4.742626377731522 2315 78-79 -3.8982469107508884 2315 80-81 -3.664686775499341 2315 82-83 -6.848729608440923 2315 84-85 -4.809425193449947 2315 86-87 -5.337442594773485 2315 88-89 -3.540331282466987 2315 90-91 -3.1994153774827616 2315 92-93 -3.932136405037433 2315 94-95 -1.9256114701778522 2315 96-97 -0.8052821448169745 2315 98-99 -2.604992166853421 2315 100-101 -1.8150989225418712 2316 1 -0.3452702217879349 2316 2 -0.2862722530235544 2316 3 -0.768696108608296 2316 4 -0.16594413667904462 2316 5 -0.029664262921926365 2316 6 -2.2569567093115737 2316 7 -0.21778585716847942 2316 8 -0.22151810301649277 2316 9 0.22957803192488768 2316 10-11 -0.05604473358170736 2316 12-13 -0.18764677319391154 2316 14-15 -0.66882900540827 2316 16-17 -1.473521223254977 2316 18-19 -0.19542196642250786 2316 20-21 -3.8294492615663813 2316 22-23 -3.63143507708498 2316 24-25 -2.478545848660758 2316 26-27 -1.9669239689076505 2316 28-29 -1.1830477399408679 2316 30-31 -0.1268941665163652 2316 32-33 -1.715266729251013 2316 34-35 -2.3819875222379707 2316 36-37 -4.989906687159014 2316 38-39 -2.5721321782793467 2316 40-41 -1.4715506534087766 2316 42-43 -2.6643769534100272 2316 44-45 -0.9970616605524043 2316 46-47 -1.1455666822331807 2316 48-49 -0.8224090463000806 2316 50-51 -0.7705473040951745 2316 52-53 -3.003592616728099 2316 54-55 -1.6634023397800561 2316 56-57 -0.6897598318472475 2316 58-59 -0.24836415078902974 2316 60-61 -1.276623306977271 2316 62-63 -0.9105206627221918 2316 64-65 -0.02507652534206173 2316 66-67 1.146568497171124 2316 68-69 3.3108184164353283 2316 70-71 3.651254825632673 2316 72-73 1.9442249286544033 2316 74-75 0.9175980099908436 2316 76-77 -0.3689197435257974 2316 78-79 -0.027500493443302076 2316 80-81 -0.192075698201716 2316 82-83 -0.03024346889574403 2316 84-85 0.23489506328661847 2316 86-87 0.05247606842576502 2316 88-89 0.5522654087298271 2316 90-91 0.6389623129947495 2316 92-93 0.668566300656142 2316 94-95 1.0330008244052244 2316 96-97 1.1701231559078629 2316 98-99 -0.03430661381850442 2316 100-101 0.1478845334421912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 28.0 4 104.0 5 247.0 6 562.0 7 1330.0 8 2639.0 9 4338.0 10 6926.0 11 10292.0 12 13196.0 13 16400.0 14 20377.0 15 25952.0 16 31188.0 17 35095.0 18 31248.0 19 25566.0 20 24066.0 21 24254.0 22 25744.0 23 29329.0 24 35559.0 25 44042.0 26 55043.0 27 68619.0 28 84439.0 29 99973.0 30 117538.0 31 136270.0 32 158715.0 33 183564.0 34 213538.0 35 244663.0 36 252797.0 37 215009.0 38 91500.0 39 5415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47845293693161 20.77867070948456 15.76639022186248 20.976486131721344 2 15.642071003383155 22.450301914264912 39.268082737356 22.639544344995933 3 19.448272909240767 27.053295227085407 30.87132718923605 22.627104674437774 4 12.653029767830192 18.57058754268221 37.177012084818514 31.599370604669087 5 12.46700488488264 39.46817333476624 33.58199465398855 14.482827126362565 6 29.310640848613296 37.886387720368646 17.58590496986823 15.217066461149825 7 26.56206715690997 35.31023409652869 20.457745951211155 17.669952795350184 8 26.215728460871517 34.97280113034263 19.389315264977576 19.42215514380827 9 26.45665414298406 17.325669267739208 20.67811994904895 35.53955664022778 10-11 24.40927822912987 28.17004377936911 26.498699463729487 20.921978527771536 12-13 26.282628474786723 25.77425259304454 27.55925200432442 20.38386692784432 14-15 22.96575789687764 26.58822773835137 27.496184023206276 22.949830341564713 16-17 20.36874970028704 30.175486220054392 28.09160341295993 21.364160666698638 18-19 20.089340859613554 27.909791678574997 31.984485877948636 20.016381583862813 20-21 21.489818239503062 26.683167967096733 31.915309023672343 19.911704769727866 22-23 20.902325979683802 26.970810185928517 31.63698446849277 20.489879365894907 24-25 20.684456718367 27.12865140275949 32.073301007246606 20.1135908716269 26-27 20.650005876526155 27.12202001786721 32.111822264733966 20.11615184087266 28-29 20.077386659655 27.292661692554294 32.541024008235844 20.088927639554857 30-31 20.28376222154237 27.44793193166362 32.200388229125835 20.067917617668172 32-33 20.149114248773383 27.555498748496237 31.861117016618852 20.43426998611153 34-35 20.61502360987558 27.300274155027655 31.805708547834755 20.278993687262016 36-37 20.74282068793932 27.133081849097028 31.911596640600337 20.21250082236331 38-39 20.611283469411504 27.291932624322456 32.0556079285723 20.041175977693737 40-41 20.528332087351338 27.356663488841033 32.10670549786697 20.00829892594066 42-43 20.269034847663356 27.65604942663475 31.542696477516497 20.532219248185395 44-45 20.510272107837686 27.889012248613486 30.995084397123453 20.60563124642537 46-47 20.624707355933403 27.9096240119747 30.436761559239734 21.028907072852164 48-49 20.816222530477997 28.076681826821925 30.227890928315386 20.879204714384688 50-51 20.989414710372454 28.358572935979954 30.01836932194658 20.63364303170101 52-53 20.99036811221041 28.527994648975035 29.840105563621943 20.641531675192613 54-55 20.48618817196469 28.375601003250196 30.064601154920233 21.073609669864876 56-57 20.903563020451745 28.92235608959732 29.328705453677905 20.84537543627303 58-59 20.935679551625448 28.33068651144307 29.622679605796616 21.110954331134867 60-61 20.94615165574399 28.245898375193494 29.995953505998017 20.811996463064503 62-63 21.185238722097065 28.226425741999833 29.85667704047412 20.73165849542898 64-65 20.938976423468116 28.225384485612672 29.9530755851743 20.882563505744912 66-67 20.464271894750418 28.373812287716582 30.20078249279849 20.96113332473451 68-69 20.666101093932195 28.254911731591353 30.27368699110054 20.805300183375916 70-71 20.287656529826123 28.227976418437308 30.482731687255065 21.00163536448151 72-73 20.804598169980213 28.267715369087764 29.50108851034981 21.42659795058221 74-75 20.444812440358184 28.61450038730331 29.603265482538333 21.33742168980017 76-77 20.696887730970733 28.514548166761628 29.54688958304027 21.24167451922737 78-79 20.459112113765975 28.448109934440392 29.646557023117037 21.4462209286766 80-81 20.320572956026815 28.97894574138624 29.35566852505441 21.344812777532532 82-83 20.508654185800072 28.88751164060242 29.02670648555495 21.57712768804256 84-85 20.369823276923242 28.56088971666506 29.01739400361795 22.051893002793744 86-87 20.490338756851116 28.57836784098436 29.245892542673758 21.685400859490773 88-89 20.644329687239757 28.27738455761554 29.168755745412554 21.909530009732144 90-91 20.20642437528902 28.579349855276003 29.409928579992123 21.804297189442856 92-93 20.195441970912775 28.736747637685202 29.23490073118401 21.832909660218014 94-95 19.77057734352036 28.56343183510233 29.35743204536531 22.308558776011996 96-97 19.664817247362013 28.294227176894115 29.378087878672144 22.662867697071725 98-99 20.03009123506599 28.207524607303387 28.980039023968285 22.78234513366234 100-101 20.01091895884516 28.188341335217387 29.253153919852704 22.54758578608475 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9275.0 1 6156.5 2 3015.0 3 3133.0 4 2872.0 5 2852.5 6 4713.5 7 8625.5 8 11949.5 9 13032.0 10 11673.5 11 9072.5 12 7660.5 13 7141.0 14 6740.5 15 6374.0 16 6203.5 17 6174.0 18 6333.0 19 6647.5 20 7017.0 21 7720.0 22 8745.5 23 9826.0 24 11219.5 25 13179.0 26 16026.0 27 19338.0 28 22601.0 29 26448.0 30 30387.0 31 34433.0 32 39594.0 33 45392.0 34 51009.5 35 56286.0 36 62697.5 37 68738.0 38 71619.5 39 72336.0 40 72773.0 41 74192.5 42 75752.0 43 77067.0 44 78694.5 45 80117.5 46 81295.0 47 86005.5 48 99319.0 49 99124.5 50 84509.5 51 75604.0 52 68949.5 53 64193.5 54 60354.0 55 55586.0 56 49961.5 57 44452.0 58 39482.5 59 34626.5 60 29618.0 61 24155.0 62 18936.0 63 14322.5 64 10239.0 65 7328.0 66 5336.5 67 3982.0 68 2801.0 69 1881.5 70 1406.5 71 1030.0 72 781.5 73 663.5 74 505.5 75 338.0 76 234.5 77 137.0 78 78.0 79 53.5 80 31.5 81 15.0 82 11.0 83 7.0 84 2.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024405125076266018 2 0.02033760423022168 3 0.0 4 0.0 5 8.563201781145971E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.99712062340109E-4 18-19 0.0010918082270961111 20-21 6.422401335859478E-5 22-23 0.0 24-25 0.0 26-27 4.923841024158933E-4 28-29 0.017897091722595078 30-31 0.004217376877214391 32-33 0.007899553643107158 34-35 0.009312481936996244 36-37 0.10106718902197533 38-39 0.10355051753850764 40-41 0.11744431242841698 42-43 0.10361474155186626 44-45 0.09807006839857424 46-47 0.09944018068355757 48-49 0.10170942915556126 50-51 0.10006101281269067 52-53 0.07777528017725827 54-55 0.0668357899018443 56-57 0.037870759877118056 58-59 0.016462755424253128 60-61 0.009419521959260567 62-63 0.0019481284052107084 64-65 0.005009473041970393 66-67 0.4142448861629363 68-69 0.8027787589779819 70-71 0.8905943932436338 72-73 0.2772122496601479 74-75 0.14658060648876614 76-77 0.014964195112552583 78-79 0.009398113954807703 80-81 0.0016270083384177345 82-83 0.0 84-85 0.0 86-87 2.568960534343791E-4 88-89 6.850561424916777E-4 90-91 0.004067520846044336 92-93 0.002055168427475033 94-95 0.22093060595356606 96-97 0.22666795114693383 98-99 0.014707299059118203 100-101 0.008028001669824349 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2335575.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.14071248515738 #Duplication Level Percentage of deduplicated Percentage of total 1 71.56716936558462 34.45294523805172 2 14.773500268291823 14.224136576304641 3 5.651747770228852 8.162374933356494 4 2.718869778599716 5.235533131846097 5 1.5235665891669545 3.6672790560539124 6 0.9429613967219382 2.723690009051595 7 0.5886211548088476 1.9835649243433828 8 0.4079426608361522 1.5710920276594618 9 0.2873076420039019 1.2448075129648528 >10 1.3714776941284594 11.688477689276061 >50 0.10047039663525885 3.369265453655098 >100 0.06033605105802894 5.257032974971536 >500 0.003869507063102398 1.3248465699599175 >1k 0.0016197936543218831 1.7857833139391297 >5k 1.7997707270243148E-4 0.45110284915938476 >10k+ 3.5995414540486296E-4 2.858067739406631 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21592 0.924483264292519 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19769 0.8464296800573734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14045 0.6013508450809757 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10568 0.45247958211575307 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5246 0.2246127827194588 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5167 0.22123031801590615 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4821 0.20641597893452363 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4705 0.20144932190145895 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4399 0.18834762317630563 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2987 0.12789141860141506 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2557 0.10948053477195124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.281600890572985E-5 0.0 12-13 0.0 0.0 0.0 4.281600890572985E-5 0.0 14-15 0.0 0.0 0.0 6.422401335859478E-5 0.0 16-17 0.0 0.0 0.0 2.568960534343791E-4 0.0 18-19 0.0 0.0 0.0 3.2112006679297386E-4 0.0 20-21 0.0 0.0 0.0 5.566081157744881E-4 0.0 22-23 0.0 0.0 0.0 0.0012630722627190306 0.0 24-25 0.0 0.0 0.0 0.001969536409663573 0.0 26-27 0.0 0.0 0.0 0.002676000556608116 0.0 28-29 0.0 0.0 0.0 0.0048168010018946085 0.0 30-31 0.0 0.0 0.0 0.01029725014182803 0.0 32-33 0.0 0.0 0.0 0.019352836025389893 0.0 34-35 0.0 0.0 0.0 0.0348522312492641 0.0 36-37 0.0 2.1408004452864925E-5 0.0 0.05852948417413271 0.0 38-39 0.0 4.281600890572985E-5 0.0 0.09126232298256318 0.0 40-41 0.0 4.281600890572985E-5 0.0 0.12834098669492525 0.0 42-43 0.0 4.281600890572985E-5 0.0 0.16458473823362557 0.0 44-45 0.0 4.281600890572985E-5 0.0 0.21114714791860678 0.0 46-47 0.0 4.281600890572985E-5 0.0 0.26890594393243633 0.0 48-49 0.0 4.281600890572985E-5 0.0 0.33492822966507174 0.0 50-51 0.0 4.281600890572985E-5 0.0 0.4034980679275981 0.0 52-53 0.0 4.281600890572985E-5 0.0 0.4681074253663445 0.0 54-55 0.0 4.281600890572985E-5 0.0 0.5379403358915898 0.0 56-57 0.0 4.281600890572985E-5 0.0 0.6217312653201031 0.0 58-59 0.0 4.281600890572985E-5 0.0 0.7057362747931452 0.0 60-61 0.0 4.281600890572985E-5 0.0 0.7964633976643867 0.0 62-63 4.281600890572985E-5 4.281600890572985E-5 0.0 0.8844717039701144 0.0 64-65 4.281600890572985E-5 4.281600890572985E-5 0.0 0.9764190830951693 0.0 66-67 4.281600890572985E-5 4.281600890572985E-5 0.0 1.0716847029104182 0.0 68-69 4.281600890572985E-5 4.281600890572985E-5 0.0 1.1907774316817057 0.0 70-71 4.281600890572985E-5 4.281600890572985E-5 0.0 1.3509307129935884 0.0 72-73 4.281600890572985E-5 4.281600890572985E-5 0.0 1.525791293364589 0.0 74-75 4.281600890572985E-5 4.281600890572985E-5 0.0 1.712640356229194 0.0 76-77 4.281600890572985E-5 4.281600890572985E-5 0.0 1.9153099343844664 0.0 78-79 4.281600890572985E-5 4.281600890572985E-5 0.0 2.1554863363411583 0.0 80-81 4.281600890572985E-5 4.281600890572985E-5 0.0 2.4172634147907903 0.0 82-83 4.281600890572985E-5 4.281600890572985E-5 0.0 2.6879676310972673 0.0 84-85 4.281600890572985E-5 4.281600890572985E-5 0.0 2.964366376588206 0.0 86-87 4.281600890572985E-5 4.281600890572985E-5 0.0 3.247958211575308 0.0 88-89 4.281600890572985E-5 4.281600890572985E-5 0.0 3.5511383706367807 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20325 0.0 25.836798 1 GTATCAA 27270 0.0 24.071 1 GAGTACT 16135 0.0 23.004538 12-13 GTACATG 17790 0.0 22.72493 1 TACATGG 17880 0.0 22.55125 2 GTACTTT 17200 0.0 21.731617 14-15 ACATGGG 17995 0.0 21.429237 3 AGTACTT 16640 0.0 20.797464 12-13 TATCAAC 32580 0.0 20.0695 2 CATGGGA 13095 0.0 19.93335 4 ATCAACG 32785 0.0 19.911694 3 TCAACGC 33180 0.0 19.67465 4 ATGGGAG 3850 0.0 19.564583 5 ACTTTTT 19760 0.0 19.479593 16-17 CAACGCA 33550 0.0 19.40119 5 AACGCAG 34180 0.0 19.057451 6 TACTTTT 18760 0.0 18.497723 14-15 CATTCCG 285 4.4105036E-8 18.284452 9 GTCGGGA 475 0.0 17.955086 2 TATCACG 160 0.001211647 17.768053 2 >>END_MODULE