FastQCFastQC Report
Fri 27 May 2016
SRR939466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330936
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159150.6827729289864672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119780.513870822708131No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84550.3627298218398103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69710.299064410176856No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38420.16482649030260804No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA30540.13102032831446253No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30070.12900397093699698No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28280.12132465241430909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27150.1164768144642324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA256800.025.7883381
GTACATG157700.024.850951
TACATGG155700.023.9405122
GAGTACT132400.023.86556212-13
GTATCGA2602.9467628E-1023.2788521
GGTATCA189200.023.1127511
ACATGGG161250.022.301453
GTACTTT145900.022.0501914-15
TATCAAC298600.022.0140572
AGTACTT137150.021.618812-13
ATCAACG306500.021.5494373
TCAACGC310400.021.294044
ATCGACG2508.141797E-921.1819023
CAACGCA310650.020.9977825
AACGCAG313500.020.6191736
ACTTTTT173350.020.07733316-17
CATGGGG45750.019.5967164
CATGGGA115350.019.2657054
TCGACGC2752.7146598E-819.0755274
TATCGAC2853.0082447E-818.905372
Produced by FastQC (version 0.11.5)