FastQCFastQC Report
Fri 27 May 2016
SRR939466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330936
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211830.9087765601457956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190220.8160670220031782No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138590.5945680190275494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA104940.4502054110451767No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA50030.21463480764808643No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49720.21330486980337512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46890.20116382431778476No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44900.19262648137915414No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.1856764836100176No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT29590.1269447123387343No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24940.10699564466806469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198200.025.366451
GTATCAA263150.023.1287421
CATTCCG2406.0663297E-921.729629
GTACATG175550.021.4727211
GAGTACT158850.021.26377512-13
TACATGG176150.021.1524722
AGTACTT163000.020.7514812-13
ACATGGG175400.020.1290913
GTACTTT169400.019.89751814-15
TATCAAC316550.019.2679232
ATCAACG320600.018.990833
ATACCGT1507.8233687E-418.9640316
CATGGGA125600.018.89334
TCAACGC324350.018.7440194
CAACGCA327300.018.6279755
TACTTTT183450.018.3348514-15
AACGCAG334750.018.2558926
AACGCGG1300.007648517418.2346465
ATGGGAT49050.018.0747915
ACTTTTT194450.017.9189616-17
Produced by FastQC (version 0.11.5)