##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939466_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2330936 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.857236749528944 31.0 31.0 31.0 28.0 34.0 2 31.083492210854352 31.0 31.0 34.0 28.0 34.0 3 31.067194466085727 31.0 31.0 34.0 27.0 34.0 4 34.99033478396661 35.0 35.0 37.0 32.0 37.0 5 34.567892468948095 35.0 35.0 37.0 30.0 37.0 6 34.64754287548006 35.0 35.0 37.0 31.0 37.0 7 34.485694587925195 35.0 35.0 37.0 30.0 37.0 8 34.391781241527006 35.0 35.0 37.0 30.0 37.0 9 35.854909787312906 37.0 35.0 39.0 30.0 39.0 10-11 35.650531374520796 37.0 35.0 39.0 30.0 39.0 12-13 35.632444648844924 37.0 35.0 39.0 30.0 39.0 14-15 36.36729172315327 38.0 34.5 40.0 30.0 41.0 16-17 36.309738662923394 38.0 34.5 40.0 30.0 41.0 18-19 36.38201735268579 38.0 35.0 40.0 30.0 41.0 20-21 36.30629498193001 38.0 34.0 40.0 29.5 41.0 22-23 36.141793897387146 38.0 34.0 40.0 29.0 41.0 24-25 35.977718821966796 38.0 34.0 40.0 29.0 41.0 26-27 35.47523484128264 38.0 34.0 40.0 27.0 41.0 28-29 35.259945575511296 38.0 33.5 40.0 27.0 41.0 30-31 34.93187843853285 37.0 33.0 40.0 27.0 41.0 32-33 34.621422038185514 37.0 33.0 40.0 25.5 41.0 34-35 34.79618445122475 37.0 33.0 40.0 26.0 41.0 36-37 34.584254994560126 37.0 33.0 40.0 25.0 41.0 38-39 34.382999361629835 37.0 33.0 40.0 25.0 41.0 40-41 34.19459007025504 37.0 33.0 40.0 24.0 41.0 42-43 34.0044293365412 37.0 33.0 40.0 24.0 41.0 44-45 33.56824983611733 37.0 32.5 40.0 23.0 41.0 46-47 33.08787714463203 37.0 31.0 40.0 20.0 41.0 48-49 32.64427980862624 36.0 31.0 39.0 18.5 40.5 50-51 32.39872995225952 36.0 31.0 39.0 16.5 40.0 52-53 32.27128393915577 36.0 31.0 39.0 15.5 40.0 54-55 31.953981147487532 36.0 30.5 39.0 14.0 40.0 56-57 31.480127725514556 35.0 29.5 39.0 11.0 40.0 58-59 31.046417619359776 35.0 28.5 38.5 8.5 40.0 60-61 30.690810687208916 35.0 28.0 38.0 8.0 40.0 62-63 30.539207854698716 34.5 28.0 38.0 7.5 40.0 64-65 30.091394186712975 34.0 28.0 38.0 6.0 40.0 66-67 29.25047212793487 33.5 27.0 37.5 2.0 39.5 68-69 25.931112008223305 31.0 16.5 36.0 2.0 39.0 70-71 24.66213722727694 29.5 12.0 35.0 2.0 38.0 72-73 25.630167023032804 30.5 18.5 35.0 2.0 38.0 74-75 26.337209601636424 31.0 21.5 35.0 2.0 37.5 76-77 25.979795026547272 30.5 22.0 34.0 2.0 36.0 78-79 27.05791514653341 32.0 25.0 35.0 2.0 37.0 80-81 27.07275339177052 32.0 25.0 35.0 2.0 36.5 82-83 26.755306452000397 32.0 25.0 35.0 2.0 36.0 84-85 26.386928255430437 32.0 24.5 34.5 2.0 36.0 86-87 25.924669317390094 32.0 23.5 34.0 2.0 35.0 88-89 25.4161446303116 31.0 21.0 34.0 2.0 35.0 90-91 24.812453881187643 31.0 19.5 34.0 2.0 35.0 92-93 24.264879644915176 31.0 17.5 34.0 2.0 35.0 94-95 23.44384101493992 30.0 7.0 34.0 2.0 35.0 96-97 22.767140110238977 30.0 2.0 34.0 2.0 35.0 98-99 21.917239254960236 29.5 2.0 34.0 2.0 35.0 100-101 20.541893042108406 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2214758131760739 1101 2 0.18822279000272601 1101 3 0.29569213733654465 1101 4 0.27494709530104444 1101 5 0.4907799366245129 1101 6 0.20406386445132085 1101 7 0.3692654996665041 1101 8 0.5867562769122188 1101 9 0.6046382753088793 1101 10-11 0.6794506163458252 1101 12-13 0.6528652982776535 1101 14-15 0.6358386364880957 1101 16-17 0.6768831433118621 1101 18-19 0.546784365938727 1101 20-21 0.6427362779736896 1101 22-23 0.5802524604334707 1101 24-25 0.711008531841749 1101 26-27 0.7662607747726895 1101 28-29 0.5978127637890438 1101 30-31 0.3668764063250265 1101 32-33 0.636581335810142 1101 34-35 0.6115243131168242 1101 36-37 0.8674446471222694 1101 38-39 0.8544164039079831 1101 40-41 0.72428072211558 1101 42-43 0.5920934635878083 1101 44-45 0.7942404535672267 1101 46-47 0.6613787388460253 1101 48-49 0.5741380488856649 1101 50-51 0.33890239094043295 1101 52-53 0.1629523018375565 1101 54-55 0.23524170059491212 1101 56-57 0.4119464952827414 1101 58-59 0.41344841934645515 1101 60-61 0.3018413145838892 1101 62-63 -0.005629448250523694 1101 64-65 -0.14074949599206832 1101 66-67 -0.07190610361165639 1101 68-69 -7.145439914390529 1101 70-71 -7.763678301078738 1101 72-73 -4.86200686950685 1101 74-75 -2.7920341346210584 1101 76-77 -0.3707227868272476 1101 78-79 -0.20654676205229805 1101 80-81 -0.14959584787959201 1101 82-83 -0.36436464727950835 1101 84-85 -0.5733685184064683 1101 86-87 -0.6135116788715571 1101 88-89 -0.6828787762490549 1101 90-91 -0.6611342228665826 1101 92-93 -0.6181477884873665 1101 94-95 -0.5756100994194462 1101 96-97 -0.6395543219531525 1101 98-99 -0.6264242979930401 1101 100-101 -0.6269016755605605 1102 1 0.27245658955210317 1102 2 0.24487599278031524 1102 3 0.19273014738555716 1102 4 0.19070457199361357 1102 5 0.37165881807795387 1102 6 0.3917387276133013 1102 7 0.3933130821402102 1102 8 0.5622536071103283 1102 9 0.5175782477640283 1102 10-11 0.5382248575824846 1102 12-13 0.5028824317493701 1102 14-15 0.6040443943849212 1102 16-17 0.53898479009451 1102 18-19 0.44344950963012764 1102 20-21 0.5240485837451061 1102 22-23 0.48626314161041506 1102 24-25 0.5080737191217608 1102 26-27 0.4373676200213339 1102 28-29 0.4324657354945245 1102 30-31 0.47463043466024857 1102 32-33 0.5831701173905444 1102 34-35 0.5541012529080973 1102 36-37 0.535162133897984 1102 38-39 0.744133793528384 1102 40-41 0.6272236256416619 1102 42-43 0.4675176154809577 1102 44-45 0.49776451657638887 1102 46-47 0.3935486044171981 1102 48-49 0.5290645456355634 1102 50-51 0.3750158598185678 1102 52-53 0.16822658836959903 1102 54-55 -0.07939908074391155 1102 56-57 0.1231743842420876 1102 58-59 0.13425849776654886 1102 60-61 0.1776153569147212 1102 62-63 -0.1554233378374441 1102 64-65 -0.17229961894606305 1102 66-67 -0.1937606181173379 1102 68-69 -6.904027985030325 1102 70-71 -7.504026117119082 1102 72-73 -1.7437538304490978 1102 74-75 -0.12901103856989593 1102 76-77 0.2699558988713555 1102 78-79 -0.07109115940705024 1102 80-81 -0.2711613581353838 1102 82-83 -0.13643630063983636 1102 84-85 -0.4759418378492164 1102 86-87 -0.6111110234670285 1102 88-89 -0.6207072498471007 1102 90-91 -0.6950891040679466 1102 92-93 -0.6031863617746431 1102 94-95 -0.4402893982468896 1102 96-97 -0.5419623788861685 1102 98-99 -0.8002576464248357 1102 100-101 -0.5993112062347237 1103 1 0.2617544329728254 1103 2 0.21163805320929185 1103 3 0.3272237498685264 1103 4 0.31902507596257834 1103 5 0.42740202480872824 1103 6 0.43623589663413753 1103 7 0.48437534460942544 1103 8 0.5173826191475541 1103 9 0.5510156527993999 1103 10-11 0.6037331305516815 1103 12-13 0.6276113653354756 1103 14-15 0.6684408261941144 1103 16-17 0.7472291189178506 1103 18-19 0.801872658050236 1103 20-21 0.8223556226032045 1103 22-23 0.6768612932410463 1103 24-25 0.8497545629353738 1103 26-27 0.9400192392040978 1103 28-29 0.7921254108569329 1103 30-31 0.7648183549681491 1103 32-33 0.815192398398132 1103 34-35 0.8235538243119649 1103 36-37 1.105513523703408 1103 38-39 1.2967445977858532 1103 40-41 1.1597082850786222 1103 42-43 0.8132951302716052 1103 44-45 0.9832066369435424 1103 46-47 1.049960362167269 1103 48-49 1.1440325453354845 1103 50-51 1.0657998878320996 1103 52-53 0.6742983647851801 1103 54-55 0.5648568065865476 1103 56-57 0.7064971482898592 1103 58-59 0.8519560591701243 1103 60-61 0.8881367707640777 1103 62-63 0.6628075989858822 1103 64-65 0.5874118856497468 1103 66-67 0.5239821607560415 1103 68-69 -6.641962946891891 1103 70-71 -7.451047930852983 1103 72-73 -0.6943276393462412 1103 74-75 0.8687990531428937 1103 76-77 1.0988656285900866 1103 78-79 0.8427864873645667 1103 80-81 0.6821340815278205 1103 82-83 0.655851728762169 1103 84-85 0.4421243452686596 1103 86-87 0.3864722656819204 1103 88-89 0.5289477941020593 1103 90-91 0.3406896758582292 1103 92-93 0.2670185202498807 1103 94-95 0.4214080830526292 1103 96-97 0.40626663798272844 1103 98-99 0.5614314907018709 1103 100-101 0.6944493709976669 1104 1 0.22591527320871307 1104 2 0.30592523014216155 1104 3 0.2871789524561379 1104 4 0.2429062167746494 1104 5 0.4594535433702944 1104 6 0.387357830455727 1104 7 0.5346949527781106 1104 8 0.4801795518930163 1104 9 0.49174071290848786 1104 10-11 0.48120436735381844 1104 12-13 0.44657731991699023 1104 14-15 0.7222154770694615 1104 16-17 0.7536829286964206 1104 18-19 0.5871158769457097 1104 20-21 0.5500954286553181 1104 22-23 0.6153645462034518 1104 24-25 0.6409584972366034 1104 26-27 0.7978202765854903 1104 28-29 0.6271752352953541 1104 30-31 0.5430762367915278 1104 32-33 0.7555451972371472 1104 34-35 0.3895849642016458 1104 36-37 0.7063089314305842 1104 38-39 0.8025237560741445 1104 40-41 0.7244592243792027 1104 42-43 0.6903948744620791 1104 44-45 0.7485766537310425 1104 46-47 0.7348986542697631 1104 48-49 0.7551566467737985 1104 50-51 0.7267406642385907 1104 52-53 0.606918306244161 1104 54-55 0.7745010868455608 1104 56-57 0.7313172053550652 1104 58-59 0.907732219690601 1104 60-61 0.8860549363146539 1104 62-63 0.559117346804431 1104 64-65 0.5901266334375528 1104 66-67 0.6727957364934909 1104 68-69 -6.211462734742167 1104 70-71 -3.6807078456241378 1104 72-73 0.8360757987091105 1104 74-75 1.4457541358197936 1104 76-77 1.5572865121168178 1104 78-79 1.139030207425325 1104 80-81 0.973260458195039 1104 82-83 0.8065505161266522 1104 84-85 0.7597386908943804 1104 86-87 0.7294856987563705 1104 88-89 0.8099474804894236 1104 90-91 0.7454406766514836 1104 92-93 0.7190608456568057 1104 94-95 0.9319008853352386 1104 96-97 0.7558794647394187 1104 98-99 0.9648493303412771 1104 100-101 1.0720731864950537 1105 1 0.10801181513276603 1105 2 0.1948589449532392 1105 3 0.14937507196448863 1105 4 0.24707731457520765 1105 5 0.4218790526743419 1105 6 0.270810127822358 1105 7 0.2933306895584664 1105 8 0.3786701838089215 1105 9 0.4453173717153689 1105 10-11 0.3772215689202838 1105 12-13 0.46703257747164884 1105 14-15 0.44324523827994966 1105 16-17 0.31984431268914904 1105 18-19 0.45022978434053584 1105 20-21 0.43946085978844707 1105 22-23 0.4310786239062878 1105 24-25 0.4937365990112639 1105 26-27 0.6844793807836496 1105 28-29 0.6755550602760465 1105 30-31 0.6150518020898588 1105 32-33 0.68028354116327 1105 34-35 0.49664550435401367 1105 36-37 0.5757014410799712 1105 38-39 0.6138496961796918 1105 40-41 0.5678213667533498 1105 42-43 0.48186521807411964 1105 44-45 0.49836658939670286 1105 46-47 0.5523972305893281 1105 48-49 0.7759516817692713 1105 50-51 0.5053920138571542 1105 52-53 0.44959502367542115 1105 54-55 0.5007324846596077 1105 56-57 0.5178862003014757 1105 58-59 0.6543292994494863 1105 60-61 0.7333131221912872 1105 62-63 0.5438269045601167 1105 64-65 0.5886152070202932 1105 66-67 0.6611963430156607 1105 68-69 -2.5647321682660156 1105 70-71 -2.78020698455477 1105 72-73 0.8985166311600281 1105 74-75 1.2194009914109536 1105 76-77 1.2252710456489702 1105 78-79 0.8178740493117935 1105 80-81 0.4461021243471244 1105 82-83 0.5553148682357971 1105 84-85 0.6077386390548796 1105 86-87 0.6090312797818918 1105 88-89 0.563417715848388 1105 90-91 0.5674156701906057 1105 92-93 0.5742112145177316 1105 94-95 0.5900417835765808 1105 96-97 0.43215763245698113 1105 98-99 0.6311535779023423 1105 100-101 0.7610239778479198 1106 1 0.08803072005644452 1106 2 0.12324823868633672 1106 3 0.08323599338874388 1106 4 0.13542718612696092 1106 5 0.270144270537898 1106 6 0.1587835316963293 1106 7 0.17101115634761754 1106 8 0.3234197443402138 1106 9 0.3495690410502874 1106 10-11 0.31903715852836 1106 12-13 0.19947829120174987 1106 14-15 0.309744061183828 1106 16-17 0.27020619551369407 1106 18-19 0.3056491188712229 1106 20-21 0.36028279934622987 1106 22-23 0.21198928183394372 1106 24-25 0.1370606286031304 1106 26-27 0.34732895956139487 1106 28-29 0.23605927772943858 1106 30-31 0.28685352685645427 1106 32-33 0.45945878617496305 1106 34-35 0.31842236717669437 1106 36-37 0.539650930102404 1106 38-39 0.5059260900408873 1106 40-41 0.435339975518346 1106 42-43 0.18005175773154036 1106 44-45 0.35532802599858826 1106 46-47 0.5823567960837792 1106 48-49 0.6079043259200887 1106 50-51 0.40257487560842975 1106 52-53 0.35567226124268103 1106 54-55 0.23545185085234266 1106 56-57 0.3999299690152398 1106 58-59 0.6102361957773326 1106 60-61 0.8511710441820135 1106 62-63 0.24702321822307738 1106 64-65 0.3138732650747791 1106 66-67 0.4798781553652205 1106 68-69 -3.0437861343768446 1106 70-71 -3.2172511117359193 1106 72-73 0.6658952222570562 1106 74-75 0.9924912141130449 1106 76-77 1.1794510676616348 1106 78-79 0.7510475749108245 1106 80-81 0.5669510181906254 1106 82-83 0.38946162509060045 1106 84-85 0.45029853310853696 1106 86-87 0.458584595574429 1106 88-89 0.48369541576164465 1106 90-91 0.5156349796131892 1106 92-93 0.2825565352260391 1106 94-95 0.5614211629941543 1106 96-97 0.6770830112400823 1106 98-99 0.5521339046584401 1106 100-101 0.6640718583210443 1107 1 0.0843800639510981 1107 2 0.04432412359461679 1107 3 0.13835567857464426 1107 4 -0.05055847203237818 1107 5 0.2930134868510592 1107 6 0.21956893388247778 1107 7 0.061781373576685894 1107 8 0.23303570228060266 1107 9 0.35081884087342985 1107 10-11 0.18638652584291293 1107 12-13 0.31886329094539434 1107 14-15 0.13018384260653448 1107 16-17 0.03354003100344727 1107 18-19 0.26667634735387225 1107 20-21 0.2263818921099201 1107 22-23 0.2978208433779628 1107 24-25 0.29118651879671376 1107 26-27 0.5400416676424626 1107 28-29 0.4301654698878252 1107 30-31 0.32786003189205104 1107 32-33 0.42157092572212207 1107 34-35 0.285554910520915 1107 36-37 0.22727107577418337 1107 38-39 0.4189645182997239 1107 40-41 0.469792231519385 1107 42-43 0.15690150261286107 1107 44-45 0.4556772357035399 1107 46-47 0.535641057340996 1107 48-49 0.43190440694028354 1107 50-51 0.5214112483595699 1107 52-53 0.4242566485242776 1107 54-55 0.4281827568290453 1107 56-57 0.5790292269841792 1107 58-59 0.7319905615316138 1107 60-61 0.6625631483841126 1107 62-63 0.33444844069630975 1107 64-65 0.31386392876310865 1107 66-67 0.49237979317582514 1107 68-69 -2.5010587413852186 1107 70-71 -2.9286274233294627 1107 72-73 0.9497240025296492 1107 74-75 1.3666353591695781 1107 76-77 0.9771567719265271 1107 78-79 0.9332273410029863 1107 80-81 0.8318905410449666 1107 82-83 0.7256547709085979 1107 84-85 0.6281032086068237 1107 86-87 0.6013102162492636 1107 88-89 0.5472889906069014 1107 90-91 0.7188374294613737 1107 92-93 0.6089411735436556 1107 94-95 0.6904706694988718 1107 96-97 0.3974331007737284 1107 98-99 0.5740838898785867 1107 100-101 0.8429464118396339 1108 1 -0.0546504895272335 1108 2 0.018992841099873203 1108 3 0.058665359926781235 1108 4 0.017097936897279453 1108 5 -0.052626059365465494 1108 6 -0.02517963165279724 1108 7 0.18249968688008522 1108 8 0.20975289565601685 1108 9 0.19118480215085754 1108 10-11 0.3161005897993121 1108 12-13 0.09318716986557973 1108 14-15 0.2578467726358369 1108 16-17 0.24666835135955978 1108 18-19 0.2519533700465857 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0.976640601756877 2311 60-61 1.274350344802869 2311 62-63 1.198428909539654 2311 64-65 1.1992064010634351 2311 66-67 1.5884201936760505 2311 68-69 3.92010173749453 2311 70-71 4.883709385572661 2311 72-73 3.8749593800497024 2311 74-75 2.7880610858130375 2311 76-77 2.4944379026944716 2311 78-79 1.7389385315910708 2311 80-81 1.5142627163155886 2311 82-83 1.3152939781921553 2311 84-85 1.2629285274218915 2311 86-87 1.22651154169154 2311 88-89 1.3634591474455924 2311 90-91 1.4232922776010568 2311 92-93 1.723307366800146 2311 94-95 1.8540618294998268 2311 96-97 1.6561631773212042 2311 98-99 1.7446314313583962 2311 100-101 1.9377111538005565 2312 1 -0.1260485109810645 2312 2 -0.1399781339652577 2312 3 -0.006773486529045414 2312 4 0.061705589018352214 2312 5 -1.4633336010691806 2312 6 -0.3112326403907204 2312 7 0.11796647491178192 2312 8 0.2228374627036942 2312 9 -2.554319298021724 2312 10-11 -0.006838161089561368 2312 12-13 -1.1596502680434142 2312 14-15 -4.329406957327372 2312 16-17 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1.5920960474545574 2312 84-85 1.5453102326089052 2312 86-87 1.680941257526694 2312 88-89 1.8786376967847858 2312 90-91 1.9365696735565407 2312 92-93 2.1204472869390862 2312 94-95 2.099603291442122 2312 96-97 2.2142257302031787 2312 98-99 2.2727789912418217 2312 100-101 2.407218479345701 2313 1 -0.20333056307405428 2313 2 -0.15339081486740014 2313 3 -0.1311557956256273 2313 4 -0.05267418744198693 2313 5 0.1974773796785172 2313 6 0.1955471189009046 2313 7 -2.656206207707232 2313 8 -3.0933467355035837 2313 9 -0.8921350711999239 2313 10-11 -0.2570191981451231 2313 12-13 -0.04422191993720759 2313 14-15 -0.5513054254715044 2313 16-17 -0.17322225770281108 2313 18-19 0.13877446143360572 2313 20-21 -2.3491604822028123 2313 22-23 -0.5836413115404113 2313 24-25 0.07100432725335537 2313 26-27 -1.6044570278145258 2313 28-29 -1.6579867460573823 2313 30-31 -0.7562185429336026 2313 32-33 -0.7553091052135485 2313 34-35 -1.4610126079138084 2313 36-37 -2.244153431855281 2313 38-39 -1.9221945914996894 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2314 3 -0.11329935147506376 2314 4 -0.0774808197370902 2314 5 -0.19371020600532063 2314 6 -2.797265958861324 2314 7 -0.6931500148590146 2314 8 -0.03042517594268901 2314 9 -0.18765353732049306 2314 10-11 -0.29226600081987186 2314 12-13 -2.306769995783661 2314 14-15 -0.7673298381211779 2314 16-17 -1.917096024784513 2314 18-19 -0.3976523204272482 2314 20-21 -0.8407496545464497 2314 22-23 -0.49235288008588185 2314 24-25 -0.2557778042935368 2314 26-27 -1.9949185387145079 2314 28-29 -0.7820249522823062 2314 30-31 -0.4427299412372392 2314 32-33 -0.16977845540799308 2314 34-35 -0.2409275411348588 2314 36-37 -0.7484189520267677 2314 38-39 -0.5458554258618662 2314 40-41 -0.6288022296532887 2314 42-43 -0.6787999837706593 2314 44-45 -3.566003115961024 2314 46-47 -3.8836595000577496 2314 48-49 -3.8062959530965017 2314 50-51 -4.1990293926746105 2314 52-53 -1.2593501876810578 2314 54-55 -1.379087195472195 2314 56-57 -1.2251608617163647 2314 58-59 -2.152683259095646 2314 60-61 -1.376553405542765 2314 62-63 -1.5323313467897535 2314 64-65 -1.4268449424344603 2314 66-67 -1.2873742868009046 2314 68-69 -1.220184522880654 2314 70-71 1.4086042390952542 2314 72-73 1.1069279112977313 2314 74-75 0.7386660115677408 2314 76-77 0.7087503150724039 2314 78-79 -1.4712624958610085 2314 80-81 -1.0151387613137999 2314 82-83 0.1712856295375964 2314 84-85 0.25055168341706135 2314 86-87 0.026336418838734943 2314 88-89 0.06929689927565263 2314 90-91 0.3015400546080542 2314 92-93 -0.18927977015950503 2314 94-95 0.38253348239760143 2314 96-97 0.5348772613704718 2314 98-99 0.10839046037251876 2314 100-101 0.22464048857626295 2315 1 -0.43848806797481643 2315 2 -0.34452593547928245 2315 3 -0.438937495022536 2315 4 -0.2508515562400859 2315 5 -0.32516889759889267 2315 6 -0.205853957183308 2315 7 -0.9515425111683342 2315 8 -2.7924603182700736 2315 9 -0.7001762338072197 2315 10-11 -1.3248032358674138 2315 12-13 -0.5936968194283523 2315 14-15 -0.3710659920634001 2315 16-17 -0.38906446265777817 2315 18-19 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-1.2293910219820674 2315 84-85 -0.5993953180848308 2315 86-87 -0.2552505259179334 2315 88-89 -0.3786476876723057 2315 90-91 -0.3104244764272899 2315 92-93 -1.343984147419146 2315 94-95 -0.17273866729193088 2315 96-97 -0.22073401466043663 2315 98-99 -2.260752885353817 2315 100-101 -3.914806405647699 2316 1 -0.47706747574760655 2316 2 -0.392474739739626 2316 3 -0.8791656166442472 2316 4 -0.43738392202011767 2316 5 -0.21481274347222978 2316 6 -0.28168357372234 2316 7 -0.5252609993339306 2316 8 -1.0378715068458675 2316 9 -5.042516454808137 2316 10-11 -1.4160726532855321 2316 12-13 -0.6453506850023203 2316 14-15 -0.4126193747238247 2316 16-17 -0.35318161881924226 2316 18-19 -0.4495616239118263 2316 20-21 -3.9491137590731924 2316 22-23 -1.0967861661759741 2316 24-25 -0.38237318377804996 2316 26-27 -0.4739864606916555 2316 28-29 0.014632044939880018 2316 30-31 -4.721907867164532E-4 2316 32-33 -1.323068151824586 2316 34-35 -0.559790396589456 2316 36-37 -0.4227354845688893 2316 38-39 -5.304278777202303 2316 40-41 -4.793320038393848 2316 42-43 -3.532508981505927 2316 44-45 -2.079533679326204 2316 46-47 -2.5269679220422603 2316 48-49 -2.647894181895392 2316 50-51 -1.069224854589475 2316 52-53 -0.6330373739673405 2316 54-55 0.050054509027617655 2316 56-57 -2.991516688511698 2316 58-59 -0.5280804282860423 2316 60-61 -0.48509808931092735 2316 62-63 -0.03462998647870563 2316 64-65 0.32160685999344807 2316 66-67 0.3684493017370727 2316 68-69 2.8495439038141654 2316 70-71 4.006897568124824 2316 72-73 2.9143689325929394 2316 74-75 1.3553049561228185 2316 76-77 -1.1602528507156329 2316 78-79 0.48465093544948346 2316 80-81 0.4791184508160775 2316 82-83 0.66702558912149 2316 84-85 0.7944719982925861 2316 86-87 0.7659418098256516 2316 88-89 0.5378174952975456 2316 90-91 0.41143658559408536 2316 92-93 0.39189243882666247 2316 94-95 0.5808587742157592 2316 96-97 0.9698370608039752 2316 98-99 1.1746610705884137 2316 100-101 1.186187229948942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 25.0 4 109.0 5 274.0 6 665.0 7 1417.0 8 2875.0 9 4903.0 10 7641.0 11 10926.0 12 14141.0 13 17865.0 14 21671.0 15 27997.0 16 33204.0 17 36445.0 18 32370.0 19 27297.0 20 26731.0 21 27210.0 22 28749.0 23 32262.0 24 38312.0 25 46930.0 26 58158.0 27 72209.0 28 88463.0 29 104897.0 30 122016.0 31 141826.0 32 162942.0 33 187110.0 34 214321.0 35 237018.0 36 233366.0 37 187787.0 38 76646.0 39 4151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27206412412893 20.90762418042635 15.85832103257698 20.961990662867734 2 15.649713150453337 22.576453878798024 39.198237280571895 22.575595690176744 3 19.41126376867553 27.105505325139163 30.992631680556006 22.49059922562931 4 12.583254105055408 18.681770281795014 37.284226401352846 31.450749211796726 5 12.430242614983852 39.55685612989803 33.5952595867068 14.417641668411315 6 29.3096421351766 37.93411745324625 17.566677077362915 15.189563334214236 7 26.485969584750503 35.3332738436405 20.498675210301784 17.682081361307215 8 26.185146224520967 34.95106686755879 19.437513513884554 19.4262733940357 9 26.428567751323932 17.33942931080047 20.716270202184873 35.515732735690726 10-11 24.35151775179663 28.23715542207444 26.547012764131715 20.86431406199721 12-13 26.225494822680673 25.842751581338995 27.57480256858189 20.356951027398434 14-15 22.899513328551276 26.631490525694403 27.53241616243432 22.936579983320005 16-17 20.38567067644561 30.224998514539177 28.049082628731536 21.340248180283677 18-19 20.04689535463137 27.947117347141244 32.02029057444557 19.985696723781814 20-21 21.518351426208184 26.705430779738272 31.91539793456363 19.860819859489922 22-23 20.873598870838173 26.97710036648423 31.690235560231795 20.459065202445803 24-25 20.67672293868697 27.151398169895497 32.10958832409028 20.06229056732725 26-27 20.686447824177787 27.09650044651007 32.132100051719235 20.084951677592915 28-29 20.172945404561055 27.311769785950457 32.47372591551193 20.041558893976553 30-31 20.280741660099196 27.500189838146355 32.194576163278825 20.024492338475625 32-33 20.206253147895982 27.55521205771793 31.88463620833301 20.353898586053074 34-35 20.59372238826653 27.34311065329455 31.829417378614554 20.233749579824362 36-37 20.76466540789633 27.121727528104966 31.9628337145783 20.150773349420405 38-39 20.634827250806794 27.339119194903517 32.04842688836737 19.977626665922323 40-41 20.525227302683412 27.332887393454214 32.19207930058995 19.949806003272418 42-43 20.235187132781743 27.636473863194567 31.599774417511796 20.528564586511894 44-45 20.53233065597297 27.918260184470594 31.014974684400286 20.53443447515615 46-47 20.641257570166328 27.878219581461117 30.465498546343444 21.015024302029108 48-49 20.838670359706455 27.991498538261194 30.263821368841814 20.90600973319054 50-51 20.96652460456376 28.34967541235146 30.00122785619314 20.68257212689164 52-53 20.912978736281733 28.498949841800204 29.920065211540454 20.668006210377616 54-55 20.455204879966622 28.372767883968596 30.09800622528813 21.07402101077665 56-57 20.865153816494733 28.881930871888926 29.375939112368638 20.8769761992477 58-59 20.97340404989637 28.320610359461558 29.62011302635136 21.08587256429071 60-61 20.941248099502303 28.21105537771929 30.063937387142026 20.78375913563638 62-63 21.15937763646781 28.181849968370432 29.936695813635016 20.722076581526743 64-65 20.897161698047235 28.23942045525239 29.97258446152493 20.890833385175444 66-67 20.58088492617022 28.527020004386845 29.886446956483258 21.005648112959683 68-69 20.907740779424454 28.28322912132481 29.987092760388705 20.821937338862025 70-71 20.4948190670794 28.248660547473296 30.222220491313976 21.034299894133323 72-73 20.814441748696126 28.244503870016857 29.529831214010198 21.411223167276816 74-75 20.635212240330578 28.59753509081052 29.399641419368077 21.36761124949082 76-77 20.153105358795013 28.56768905255851 29.951332525969775 21.3278730626767 78-79 20.49755227074917 28.440728138041678 29.618156811282105 21.443562779927053 80-81 20.3056580743906 28.993155996306104 29.36827303938761 21.332912889915686 82-83 20.465179234873276 28.891637936804027 29.04419651054439 21.59898631777831 84-85 20.3642748127627 28.493167434063405 29.051441858569476 22.091115894604414 86-87 20.48776239451986 28.531538824886127 29.239294535261013 21.741404245333005 88-89 20.635107562309944 28.273803395042663 29.148047472937815 21.943041569709575 90-91 20.22669046634834 28.560776388461946 29.40229577833089 21.81023736685882 92-93 20.19312326055423 28.68264829505891 29.24770136813162 21.876527076255243 94-95 19.929908332583803 28.523972779909972 29.190736617229128 22.355382270277094 96-97 19.959499218588558 28.330461827788206 29.105757595720487 22.604281357902746 98-99 20.007663141021105 28.21810088829792 28.905702604052024 22.868533366628952 100-101 19.97168187157085 28.174950255560383 29.279593248700746 22.57377462416802 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9051.0 1 6065.0 2 3090.0 3 3216.5 4 2929.0 5 2799.0 6 4569.0 7 8520.5 8 11807.0 9 13025.5 10 11578.0 11 8730.5 12 7440.5 13 6934.0 14 6564.0 15 6319.5 16 6222.5 17 6215.5 18 6333.5 19 6604.0 20 7048.5 21 7800.5 22 8750.0 23 9909.5 24 11347.5 25 13366.0 26 16323.5 27 19757.0 28 23120.0 29 26562.5 30 30450.5 31 34672.5 32 39624.0 33 45195.0 34 50826.0 35 56307.5 36 62712.0 37 68589.0 38 71706.0 39 72441.5 40 72325.0 41 73724.0 42 75450.0 43 76795.5 44 78515.5 45 79556.0 46 80723.0 47 85607.5 48 98993.0 49 98906.0 50 84281.5 51 75440.0 52 68598.0 53 64217.5 54 60656.5 55 55404.0 56 49732.5 57 44260.0 58 39243.5 59 34593.0 60 29493.0 61 23904.5 62 18897.0 63 14326.0 64 10071.0 65 7389.0 66 5555.0 67 4049.5 68 2867.5 69 1943.0 70 1390.5 71 1031.0 72 779.0 73 673.0 74 537.0 75 372.5 76 235.5 77 145.5 78 101.5 79 62.5 80 35.5 81 27.0 82 14.5 83 6.5 84 4.5 85 2.5 86 0.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019562956683495385 2 0.019133944475523995 3 4.71913428768529E-4 4 0.09532651261124286 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.007979627068267854 12-13 0.0 14-15 0.0 16-17 4.0756159757282057E-4 18-19 6.43518311957085E-5 20-21 0.0 22-23 1.501542727899865E-4 24-25 0.003668054378155385 26-27 0.0034964494949668284 28-29 0.0011583329615227531 30-31 1.28703662391417E-4 32-33 6.00617091159946E-4 34-35 0.0028100299622126046 36-37 0.08337852261923964 38-39 0.09742867243030268 40-41 0.07606386447332744 42-43 0.07956031396829429 44-45 0.07887389443554006 46-47 0.07762975903242303 48-49 0.07248161253676635 50-51 0.07177374239361355 52-53 0.055213871165917895 54-55 0.04650492334409868 56-57 0.026191195296653362 58-59 0.021708017723352336 60-61 0.012720211966351714 62-63 0.0020378079878641026 64-65 0.0060490721323966 66-67 0.151076649037125 68-69 0.7011561020980414 70-71 0.85847487876115 72-73 0.34932319034070436 74-75 0.05126695885258111 76-77 0.19020256240411576 78-79 0.013149224174323104 80-81 0.0027242275206183267 82-83 0.001201234182319892 84-85 1.501542727899865E-4 86-87 0.002016357377465533 88-89 3.43209766377112E-4 90-91 0.003968362923735358 92-93 0.003989813534133927 94-95 0.07295352596553488 96-97 0.036787796833546695 98-99 0.012827465018344563 100-101 0.011733483888017518 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2330936.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.120178969158054 #Duplication Level Percentage of deduplicated Percentage of total 1 72.31768796191419 35.52257775324952 2 14.453425156409027 14.199096608002854 3 5.551615113313527 8.180889838015293 4 2.610278062439662 5.128693023452132 5 1.4387623821959798 3.5336132853779354 6 0.9087846850572835 2.678379982466622 7 0.5739462585953881 1.9734640058618893 8 0.40783617768744795 1.602638883048383 9 0.279398768777762 1.235170577351347 >10 1.2999943503792488 11.358781479126169 >50 0.09610081407741118 3.3091824545114896 >100 0.05641756594913172 5.023371161274027 >500 0.0036286281749099724 1.2741035770954166 >1k 0.001681559398128958 1.956019392242045 >5k 8.850312621731358E-5 0.21749488863819508 >10k+ 3.540125048692543E-4 2.8065230902867477 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21183 0.9087765601457956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19022 0.8160670220031782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13859 0.5945680190275494 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10494 0.4502054110451767 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5003 0.21463480764808643 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4972 0.21330486980337512 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4689 0.20116382431778476 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4490 0.19262648137915414 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4328 0.1856764836100176 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2959 0.1269447123387343 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2494 0.10699564466806469 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.14506103985695E-5 0.0 12-13 0.0 0.0 0.0 6.43518311957085E-5 0.0 14-15 0.0 0.0 0.0 8.5802441594278E-5 0.0 16-17 0.0 2.14506103985695E-5 0.0 2.14506103985695E-4 0.0 18-19 0.0 4.2901220797139E-5 0.0 2.57407324782834E-4 0.0 20-21 0.0 4.2901220797139E-5 0.0 3.8611098717425104E-4 0.0 22-23 0.0 4.2901220797139E-5 0.0 0.0010939811303270447 0.0 24-25 0.0 4.2901220797139E-5 0.0 0.0017589500526826991 0.0 26-27 0.0 4.2901220797139E-5 0.0 0.0028743817934083135 0.0 28-29 0.0 4.2901220797139E-5 0.0 0.00512669588525811 0.0 30-31 0.0 4.2901220797139E-5 0.0 0.010489348484900487 0.0 32-33 0.0 4.2901220797139E-5 0.0 0.020485332930633874 0.0 34-35 0.0 4.2901220797139E-5 0.0 0.035028846780864 0.0 36-37 0.0 4.2901220797139E-5 0.0 0.05931093775204467 0.0 38-39 0.0 4.2901220797139E-5 0.0 0.09180861250587746 0.0 40-41 0.0 4.2901220797139E-5 0.0 0.12855350811862704 0.0 42-43 0.0 4.2901220797139E-5 0.0 0.16529840373137658 0.0 44-45 0.0 4.2901220797139E-5 0.0 0.20777061232054417 0.0 46-47 0.0 4.2901220797139E-5 0.0 0.26279142799287497 0.0 48-49 0.0 4.2901220797139E-5 0.0 0.32600637683745926 0.0 50-51 0.0 4.2901220797139E-5 0.0 0.39402626241132316 0.0 52-53 0.0 4.2901220797139E-5 0.0 0.45994398816612725 0.0 54-55 0.0 4.2901220797139E-5 0.0 0.5321467427677122 0.0 56-57 0.0 4.2901220797139E-5 0.0 0.6187857581675344 0.0 58-59 0.0 4.2901220797139E-5 0.0 0.7085351120751493 0.0 60-61 0.0 4.2901220797139E-5 0.0 0.805062858868712 0.0 62-63 0.0 6.43518311957085E-5 0.0 0.8973004835825609 0.0 64-65 0.0 1.28703662391417E-4 0.0 0.9920478297130424 0.0 66-67 0.0 1.28703662391417E-4 0.0 1.0972845243284244 0.0 68-69 0.0 1.28703662391417E-4 0.0 1.2188236828467192 0.0 70-71 0.0 1.28703662391417E-4 0.0 1.3729034173396437 0.0 72-73 0.0 1.501542727899865E-4 0.0 1.5412692583580159 0.0 74-75 0.0 1.71604883188556E-4 0.0 1.7224411137843338 0.0 76-77 0.0 1.71604883188556E-4 0.0 1.9239910490892929 0.0 78-79 0.0 1.71604883188556E-4 0.0 2.16245748489019 0.0 80-81 0.0 1.71604883188556E-4 0.0 2.4280803934556765 0.0 82-83 0.0 1.71604883188556E-4 0.0 2.702626755946967 0.0 84-85 0.0 1.71604883188556E-4 0.0 2.990729904210154 0.0 86-87 0.0 1.71604883188556E-4 0.0 3.2910384497901273 0.0 88-89 0.0 1.71604883188556E-4 0.0 3.6040672073364517 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19820 0.0 25.36645 1 GTATCAA 26315 0.0 23.128742 1 CATTCCG 240 6.0663297E-9 21.72962 9 GTACATG 17555 0.0 21.472721 1 GAGTACT 15885 0.0 21.263775 12-13 TACATGG 17615 0.0 21.152472 2 AGTACTT 16300 0.0 20.75148 12-13 ACATGGG 17540 0.0 20.129091 3 GTACTTT 16940 0.0 19.897518 14-15 TATCAAC 31655 0.0 19.267923 2 ATCAACG 32060 0.0 18.99083 3 ATACCGT 150 7.8233687E-4 18.964031 6 CATGGGA 12560 0.0 18.8933 4 TCAACGC 32435 0.0 18.744019 4 CAACGCA 32730 0.0 18.627975 5 TACTTTT 18345 0.0 18.33485 14-15 AACGCAG 33475 0.0 18.255892 6 AACGCGG 130 0.0076485174 18.234646 5 ATGGGAT 4905 0.0 18.074791 5 ACTTTTT 19445 0.0 17.91896 16-17 >>END_MODULE