FastQCFastQC Report
Fri 27 May 2016
SRR939465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013008
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146880.7296543282490681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112360.5581696645020785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79210.3934907362514207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57670.28648669056456805No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA27050.1343760183764794No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25400.1261793296400213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.12563288372425743No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23320.11584653414194082No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20490.10178797103637938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117950.027.0396461
GTATCAA211650.026.3587111
TACATGG121700.025.637592
GGTATCA153900.025.0109621
GAGTACT116850.024.5360312-13
ACATGGG125850.023.707043
CATGGGA82800.022.4252094
TATCAAC251400.021.9905242
GTACTTT131450.021.84052714-15
AGTACTT126350.021.61068212-13
ATCAACG256750.021.4874343
TCAACGC259000.021.4335144
TAACGCA3151.2732926E-1121.269274
CAACGCA259650.021.252895
AACGCAG263250.021.1580526
ATGGGAT34000.020.7316595
CCTTTAG7900.020.2488881
ACTTTTT156400.020.10548616-17
CATGGGG35050.020.070794
CATTCCG5200.019.804859
Produced by FastQC (version 0.11.5)