FastQCFastQC Report
Fri 27 May 2016
SRR939465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013008
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202661.0067520844427842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180890.8986054700229706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131760.6545428532822523No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83910.416838879924968No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42210.20968620094902754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41210.2047185108057196No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37990.1887225485442681No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA37510.18633805727548028No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31440.15618417810560117No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28980.14396366035306368No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23020.11435622709894844No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT21450.10655695357395499No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20690.10278150906504098No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20290.1007944330077178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174900.026.176351
GTACATG140050.024.2637441
GTATCAA235300.024.2306731
TACATGG145050.023.3229542
GAGTACT140300.023.17926412-13
ACATGGG145200.022.4133573
GTACTTT150700.021.54819914-15
CATGGGT13150.021.2541164
AGTACTT147800.021.2498512-13
CATGGGA96150.020.692684
TAGGACA10750.019.8299144
ATCAACG285750.019.8106373
ACTTTTT169150.019.79995316-17
TATCAAC288050.019.7386052
TCAACGC286650.019.731914
CAACGCA288700.019.6082085
TACGGGT2201.3811696E-619.3792344
AACGCAG294100.019.3287166
CATTCCG5450.019.1224259
CATGGGG39600.019.0203594
Produced by FastQC (version 0.11.5)