FastQCFastQC Report
Fri 27 May 2016
SRR939464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018621
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144500.7158352162193894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104750.5189186082974466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79240.39254520784238345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56480.2797949689416686No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25050.12409461706779033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.12171675614194045No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23810.1179518096760115No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22310.11052099428273064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122900.028.3159941
GTATCAA207500.027.2896461
TACATGG128250.027.0732062
ACATGGG131250.025.9561923
GTATAGG7600.025.806471
GGTATCA151000.025.1114621
GAGTACT118950.024.1494812-13
CATGGGG36450.023.4152954
CATGGGA88600.022.8717484
TATCAAC249150.022.5526642
AGTACTT127450.021.99191512-13
ATCAACG256000.021.9914023
TCAACGC258000.021.9368934
GTACTTT135350.021.87950914-15
CAACGCA257750.021.8550975
AACGCAG260550.021.586226
CATTCCG6050.020.8387589
ACTTTTT159500.020.65672716-17
GTATCGA1956.309636E-620.6316051
ATGGGAT34700.020.5545085
Produced by FastQC (version 0.11.5)