FastQCFastQC Report
Fri 27 May 2016
SRR939464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018621
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202221.001772992552837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176820.8759445185599476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129610.6420719887487547No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83900.41563027433084265No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42120.20865729624332652No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39980.19805599961557913No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35030.1735343088177523No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35010.17343523127917523No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29460.14594121432403606No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28260.13999656200941138No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22140.10967883520482546No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT20260.10036554657858011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG5600.025.3967429
GGTATCA173050.025.241651
GTATCAA234600.024.7311731
GTACATG134900.024.0386641
TACATGG138800.022.8786532
GAGTACT144850.022.6944112-13
ACATGGG138150.022.4636653
CATGGGT14100.022.214844
AGTACTT150600.021.733512-13
GTACTTT154950.021.0315914-15
GTATACG1150.00373379220.6430441
CCTTTAG7600.020.6158811
CATGGGA91350.020.5213994
TATCAAC283600.020.2522352
TTAGGCA7300.020.1538124
ATCAACG285100.020.10593
TCAACGC285950.020.0325184
CAACGCA287450.019.82385
AACGCAG291050.019.5297366
AGTACAT103100.019.4288732
Produced by FastQC (version 0.11.5)