##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939464_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018621 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.88361609237197 31.0 31.0 31.0 28.0 34.0 2 31.120946428279503 31.0 31.0 34.0 28.0 34.0 3 31.101091289548656 31.0 31.0 34.0 28.0 34.0 4 35.030447022992426 35.0 35.0 37.0 32.0 37.0 5 34.62524515498452 35.0 35.0 37.0 32.0 37.0 6 34.693267829869995 35.0 35.0 37.0 31.0 37.0 7 34.53385504262563 35.0 35.0 37.0 30.0 37.0 8 34.44285182805489 35.0 35.0 37.0 30.0 37.0 9 35.89568225040758 37.0 35.0 39.0 30.0 39.0 10-11 35.704625831198626 37.0 35.0 39.0 30.0 39.0 12-13 35.69651261925839 37.0 35.0 39.0 30.0 39.0 14-15 36.430730186597685 38.0 35.0 40.0 30.0 41.0 16-17 36.37076424945545 38.0 35.0 40.0 30.0 41.0 18-19 36.46906898323162 38.0 35.0 40.0 30.0 41.0 20-21 36.3921575669727 38.0 34.5 40.0 30.0 41.0 22-23 36.21731072846265 38.0 34.0 40.0 29.5 41.0 24-25 36.05456769745287 38.0 34.0 40.0 29.0 41.0 26-27 35.56224026204028 38.0 34.0 40.0 27.0 41.0 28-29 35.34315703641248 38.0 34.0 40.0 27.0 41.0 30-31 34.9987706954401 37.0 33.0 40.0 27.0 41.0 32-33 34.65257693247024 37.0 33.0 40.0 25.5 41.0 34-35 34.80201558390604 37.0 33.0 40.0 26.0 41.0 36-37 34.57837058070832 37.0 33.0 40.0 25.0 41.0 38-39 34.356357384570956 37.0 33.0 40.0 25.0 41.0 40-41 34.16854303011809 37.0 33.0 40.0 24.0 41.0 42-43 33.95042382894064 37.0 33.0 40.0 24.0 41.0 44-45 33.4708704605768 37.0 32.0 40.0 22.5 41.0 46-47 32.95047980774994 37.0 31.0 40.0 20.0 41.0 48-49 32.4776944260463 36.0 31.0 39.0 16.5 40.5 50-51 32.22710206621253 36.0 31.0 39.0 15.0 40.0 52-53 32.10666712572593 36.0 31.0 39.0 14.5 40.0 54-55 31.77087625661281 35.5 30.0 39.0 11.0 40.0 56-57 31.297993531227505 35.0 29.5 39.0 8.5 40.0 58-59 30.87755353778644 35.0 28.5 38.5 8.0 40.0 60-61 30.52236774510916 35.0 28.0 38.0 7.0 40.0 62-63 30.368675942636088 34.0 28.0 38.0 6.0 40.0 64-65 29.926990257210242 34.0 28.0 38.0 2.0 40.0 66-67 29.085644853590644 33.5 27.0 37.5 2.0 39.5 68-69 25.7195595408945 31.0 15.5 36.0 2.0 39.0 70-71 24.44241018992669 29.5 11.5 35.0 2.0 38.0 72-73 25.44193288388459 30.5 16.5 35.0 2.0 38.0 74-75 26.16391709984192 31.0 21.0 35.0 2.0 37.5 76-77 25.801189277234307 30.5 21.0 34.0 2.0 36.0 78-79 26.888113717235676 32.0 24.5 35.0 2.0 37.0 80-81 26.886545815187695 32.0 25.0 35.0 2.0 36.5 82-83 26.56111548428358 32.0 24.5 35.0 2.0 36.0 84-85 26.180021658349933 32.0 23.5 34.5 2.0 36.0 86-87 25.7401877321201 32.0 22.5 34.0 2.0 35.0 88-89 25.238938612052486 31.0 20.0 34.0 2.0 35.0 90-91 24.63498596318972 31.0 18.5 34.0 2.0 35.0 92-93 24.087200866333998 31.0 14.0 34.0 2.0 35.0 94-95 23.248803762568606 30.0 4.5 34.0 2.0 35.0 96-97 22.587070827064615 30.0 2.0 34.0 2.0 35.0 98-99 21.770943877032884 29.5 2.0 34.0 2.0 35.0 100-101 20.399409052021156 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28977521040075516 1101 2 0.2691037398632794 1101 3 0.310721284596152 1101 4 0.35910238192582256 1101 5 0.5581661140310601 1101 6 0.31138851537918555 1101 7 0.5949184144858393 1101 8 0.7715184905615615 1101 9 0.5926149665724267 1101 10-11 0.7679125607406974 1101 12-13 0.7421947766439487 1101 14-15 0.7559505139179237 1101 16-17 0.6759341702344557 1101 18-19 0.5944742501816123 1101 20-21 0.7667653838471225 1101 22-23 0.8110047791766064 1101 24-25 0.6640499029679034 1101 26-27 0.8623386442848897 1101 28-29 0.8604794518628651 1101 30-31 0.6433029771422838 1101 32-33 0.9671446916845738 1101 34-35 0.8871636136298164 1101 36-37 1.1366569182843236 1101 38-39 1.0211330598710617 1101 40-41 1.0288390239219254 1101 42-43 0.7823077644765064 1101 44-45 0.8510364919577995 1101 46-47 0.7517697557627798 1101 48-49 0.8806651702350052 1101 50-51 0.6784265347807548 1101 52-53 0.5238524506872935 1101 54-55 0.6491766099683254 1101 56-57 0.6036416418538799 1101 58-59 0.7967917906821214 1101 60-61 0.5545990385051915 1101 62-63 0.07154884974785247 1101 64-65 0.07385980117800628 1101 66-67 -0.22841468491269268 1101 68-69 -6.787374776885528 1101 70-71 -7.440447032375051 1101 72-73 -4.48761444807344 1101 74-75 -2.5098887348499908 1101 76-77 -0.12001475181151733 1101 78-79 0.13825244528488412 1101 80-81 -0.0717230771873858 1101 82-83 -0.3371231545995883 1101 84-85 -0.33100826968364316 1101 86-87 -0.41711147467532683 1101 88-89 -0.4819171998925711 1101 90-91 -0.62639828673138 1101 92-93 -0.6043286461259143 1101 94-95 -0.4460398636096201 1101 96-97 -0.6292099210369528 1101 98-99 -0.6937154306924889 1101 100-101 -0.6011409892357378 1102 1 0.3296848749182182 1102 2 0.3269629581886555 1102 3 0.3547539551976264 1102 4 0.3321490573481114 1102 5 0.4889089760547378 1102 6 0.4693102664206137 1102 7 0.45792455334921556 1102 8 0.585343784496672 1102 9 0.5661496401015 1102 10-11 0.6286882289317575 1102 12-13 0.5186787140422737 1102 14-15 0.8034769453529123 1102 16-17 0.6206736250870861 1102 18-19 0.45710327288459496 1102 20-21 0.5440960204897252 1102 22-23 0.6052335501814881 1102 24-25 0.5451151062540802 1102 26-27 0.5016894324478756 1102 28-29 0.42142641894776744 1102 30-31 0.425321706395998 1102 32-33 0.5819215133613298 1102 34-35 0.5033894429327432 1102 36-37 0.7689065601708975 1102 38-39 0.9141160153960328 1102 40-41 0.7537213244989758 1102 42-43 0.6023476388782072 1102 44-45 0.6960718213751349 1102 46-47 0.8501980531489437 1102 48-49 0.802406612342871 1102 50-51 0.788292324526644 1102 52-53 0.5510627040153224 1102 54-55 0.1939909395204431 1102 56-57 0.5788960630266082 1102 58-59 0.6181517675497155 1102 60-61 0.5792787934208263 1102 62-63 0.49334426354516836 1102 64-65 0.21968337193714405 1102 66-67 0.2002007522050988 1102 68-69 -6.822592038892907 1102 70-71 -7.502773558941268 1102 72-73 -1.457883197701225 1102 74-75 0.14506486712848954 1102 76-77 0.6148480014146358 1102 78-79 0.5431386561157403 1102 80-81 0.3163189709706451 1102 82-83 0.27376290577661067 1102 84-85 -0.09855034213382652 1102 86-87 -0.1254043863803851 1102 88-89 -0.1663773497830192 1102 90-91 0.03938569956470772 1102 92-93 -0.05035774029485651 1102 94-95 0.06592382823416898 1102 96-97 -0.04133872119242099 1102 98-99 -0.3166961450699972 1102 100-101 -0.22722933667941803 1103 1 0.29897579251495543 1103 2 0.18279522323030406 1103 3 0.3043272967127564 1103 4 0.19761417433365125 1103 5 0.3760233278193965 1103 6 0.27702535758488267 1103 7 0.3365437078697582 1103 8 0.37179202348617935 1103 9 0.5649941383486876 1103 10-11 0.4778580371860599 1103 12-13 0.4174638993174611 1103 14-15 0.6478719074715968 1103 16-17 0.5743179933165976 1103 18-19 0.4802246996767394 1103 20-21 0.46015791255074845 1103 22-23 0.4514728343551795 1103 24-25 0.6354918233684188 1103 26-27 0.702422452685667 1103 28-29 0.6047614104115624 1103 30-31 0.46159887948355305 1103 32-33 0.6231590280186978 1103 34-35 0.5455320310136429 1103 36-37 0.6818402815320823 1103 38-39 0.76977493618854 1103 40-41 0.720763513413452 1103 42-43 0.4719166287502006 1103 44-45 0.6233991910081116 1103 46-47 0.7530880606219199 1103 48-49 0.8499257200813517 1103 50-51 0.496159229559062 1103 52-53 0.484685526674852 1103 54-55 0.5106052548315496 1103 56-57 0.5756997317731525 1103 58-59 0.5618800093339971 1103 60-61 0.5189563237532226 1103 62-63 0.2876905829512211 1103 64-65 0.3124757066658397 1103 66-67 0.2448368014295461 1103 68-69 -6.314879264048287 1103 70-71 -7.694385785767437 1103 72-73 -0.9655414820754054 1103 74-75 0.4114184635754832 1103 76-77 0.6522540955377174 1103 78-79 0.4589381585247132 1103 80-81 0.3621914032733571 1103 82-83 -0.015040764488830405 1103 84-85 -0.12763572243554222 1103 86-87 -0.09419065007325145 1103 88-89 0.12994828374938905 1103 90-91 -0.06258438725550874 1103 92-93 -0.016533397825860163 1103 94-95 0.34693175384461483 1103 96-97 0.45014899395949826 1103 98-99 0.3609855075519235 1103 100-101 0.37424426031438074 1104 1 0.25057648330982474 1104 2 0.3182216994473954 1104 3 0.23980640275007303 1104 4 0.217201504900558 1104 5 0.4875977872435513 1104 6 0.35654802865837354 1104 7 0.47584413376879553 1104 8 0.5141025257554119 1104 9 0.619471318423173 1104 10-11 0.49407284431637777 1104 12-13 0.24551437837794055 1104 14-15 0.6063615607375326 1104 16-17 0.6261369118003728 1104 18-19 0.4933794966608218 1104 20-21 0.44794217433587846 1104 22-23 0.3305394942374349 1104 24-25 0.40306965170862696 1104 26-27 0.6461387331471755 1104 28-29 0.5765837616051144 1104 30-31 0.5068339441582381 1104 32-33 0.5406190658088903 1104 34-35 0.42493664572995016 1104 36-37 0.7033471196114576 1104 38-39 0.7646404909205131 1104 40-41 0.7843157300933825 1104 42-43 0.7054944920250605 1104 44-45 0.8772344087877215 1104 46-47 0.797094906295797 1104 48-49 0.8111478710841311 1104 50-51 0.6967276392119643 1104 52-53 0.5565259907286091 1104 54-55 0.5215696108491166 1104 56-57 0.6918768322573783 1104 58-59 0.8183265927245422 1104 60-61 0.8782298423718764 1104 62-63 0.576167690118595 1104 64-65 0.5693337215874941 1104 66-67 0.6720101927645388 1104 68-69 -6.281071059871927 1104 70-71 -3.741409922577631 1104 72-73 0.5617846344666084 1104 74-75 1.0362361957998196 1104 76-77 1.2099091902258259 1104 78-79 0.8090914533185369 1104 80-81 0.3871231667748418 1104 82-83 0.30872794074164744 1104 84-85 0.3852783291948434 1104 86-87 0.3660728863468883 1104 88-89 0.48069432853865735 1104 90-91 0.2351898953689009 1104 92-93 0.1476317701946499 1104 94-95 0.33034690515724563 1104 96-97 0.1822189711152724 1104 98-99 0.18242972905587607 1104 100-101 0.2909087752086954 1105 1 0.14522031986542672 1105 2 0.20237164536204943 1105 3 0.2074593652559784 1105 4 0.25443679140055053 1105 5 0.4442765659811556 1105 6 0.2611098611613869 1105 7 0.3046742905401274 1105 8 0.4168043174277898 1105 9 0.4503195823596755 1105 10-11 0.40021086479263346 1105 12-13 0.31377751502342477 1105 14-15 0.34809507232398573 1105 16-17 0.3095166176065547 1105 18-19 0.4306818185219612 1105 20-21 0.5394717232183694 1105 22-23 0.5338921192812336 1105 24-25 0.45721689335587 1105 26-27 0.5826452957566772 1105 28-29 0.5439835323137103 1105 30-31 0.4109448115044003 1105 32-33 0.5701412865075781 1105 34-35 0.4233267496347608 1105 36-37 0.5330837772892991 1105 38-39 0.3208103151050352 1105 40-41 0.44797647022470954 1105 42-43 0.409155441641893 1105 44-45 0.4194285980407315 1105 46-47 0.3656561789011832 1105 48-49 0.3100076986204243 1105 50-51 0.043414245089742565 1105 52-53 0.04794717847332919 1105 54-55 0.35092909939978156 1105 56-57 0.23121459144807943 1105 58-59 0.3032198312942498 1105 60-61 0.5540364567814287 1105 62-63 0.21286174225382481 1105 64-65 0.20826898294922813 1105 66-67 0.3244387010753975 1105 68-69 -3.227874215866258 1105 70-71 -3.060636597319011 1105 72-73 0.4725897063010933 1105 74-75 0.983985559725653 1105 76-77 0.7552856547199376 1105 78-79 0.48250041091867146 1105 80-81 0.19270205139784125 1105 82-83 0.12009709990492468 1105 84-85 0.3552721969828312 1105 86-87 0.17968363561940137 1105 88-89 0.20783650825436695 1105 90-91 0.18388784774332478 1105 92-93 0.2054949037930811 1105 94-95 0.34247725846407207 1105 96-97 0.22683224183148454 1105 98-99 0.147470121669798 1105 100-101 0.21995851087607576 1106 1 0.0689087141420579 1106 2 0.15738253860823548 1106 3 0.19058366602733656 1106 4 0.09198021717927674 1106 5 0.26602184066760515 1106 6 0.27064586775621535 1106 7 0.3237698566945184 1106 8 0.3394151635680487 1106 9 0.2961006575525147 1106 10-11 0.4491951431886818 1106 12-13 0.2493660447296051 1106 14-15 0.2733865402625142 1106 16-17 0.32727996919343383 1106 18-19 0.3781027688840908 1106 20-21 0.3850334614271631 1106 22-23 0.3411758736238113 1106 24-25 0.3431778543168349 1106 26-27 0.5305844113428506 1106 28-29 0.31983291110426393 1106 30-31 0.2161941785184709 1106 32-33 0.4326243578141842 1106 34-35 0.3454797850230804 1106 36-37 0.4464486127129561 1106 38-39 0.619314701844722 1106 40-41 0.3103520811297358 1106 42-43 0.4121658049463761 1106 44-45 0.599156855710163 1106 46-47 0.6615947645456544 1106 48-49 0.7903586377948955 1106 50-51 0.6468941956285121 1106 52-53 0.5417453884480068 1106 54-55 0.4698745154040189 1106 56-57 0.5632377911183362 1106 58-59 0.6370242239221717 1106 60-61 0.7397883851804181 1106 62-63 0.5559312823821898 1106 64-65 0.5042605627643368 1106 66-67 0.5534456491999933 1106 68-69 -2.7982460582969253 1106 70-71 -2.9948516947694053 1106 72-73 0.8783071884891633 1106 74-75 1.2439411035047279 1106 76-77 1.1218492771659108 1106 78-79 1.0185379190149995 1106 80-81 0.7747267231283956 1106 82-83 0.6284981480118539 1106 84-85 0.6566912631077777 1106 86-87 0.6957644222884234 1106 88-89 0.6692523358466644 1106 90-91 0.6575320802110873 1106 92-93 0.5654489755118561 1106 94-95 1.0140944639048044 1106 96-97 1.1865080941543944 1106 98-99 1.1895916549677992 1106 100-101 1.2291647759646942 1107 1 0.056147260627962936 1107 2 0.06984238257335562 1107 3 0.06716975188372842 1107 4 0.05889867303761065 1107 5 0.21112890275492902 1107 6 0.13831783067829662 1107 7 0.056581833425724426 1107 8 0.09376772631895847 1107 9 0.10037032419023717 1107 10-11 -0.11000219090539787 1107 12-13 0.04228452539475569 1107 14-15 0.04104020815711351 1107 16-17 -0.03152717434294772 1107 18-19 0.11523340571642393 1107 20-21 0.14675065131094556 1107 22-23 0.16737972777626453 1107 24-25 0.16123504643322661 1107 26-27 0.3933491724325364 1107 28-29 0.29277797640821035 1107 30-31 0.17743290692103386 1107 32-33 0.24650439204759067 1107 34-35 0.1370079469619867 1107 36-37 0.09505539985806166 1107 38-39 0.2345374050435609 1107 40-41 0.17777587252094662 1107 42-43 0.06864896318492697 1107 44-45 0.34759516322373685 1107 46-47 0.5141377733644674 1107 48-49 0.19200539699099295 1107 50-51 0.34985240854542354 1107 52-53 0.06970587532313033 1107 54-55 0.017313211557723918 1107 56-57 0.35931686698171816 1107 58-59 0.36969562869087014 1107 60-61 0.22243074530097928 1107 62-63 0.26864126175118486 1107 64-65 0.08674994751393328 1107 66-67 0.19385812454822116 1107 68-69 -2.405109381004671 1107 70-71 -2.707144609529049 1107 72-73 0.8764805798704494 1107 74-75 1.0340895929762972 1107 76-77 0.8756372781085737 1107 78-79 0.6544256309582401 1107 80-81 0.44674515045889507 1107 82-83 0.5005556460789862 1107 84-85 0.38283185987710056 1107 86-87 0.4807521635863985 1107 88-89 0.3556860044854737 1107 90-91 0.349296216995878 1107 92-93 0.47738642925747854 1107 94-95 0.686147827502456 1107 96-97 0.6128730892550465 1107 98-99 0.7007094501379143 1107 100-101 0.9913325196606628 1108 1 0.05145056858358643 1108 2 0.078306830294256 1108 3 0.06336959640732331 1108 4 0.06801282200890313 1108 5 -0.07480700489881542 1108 6 -0.1285471710791839 1108 7 0.1445918398481325 1108 8 0.1984909137260118 1108 9 0.08430023660206842 1108 10-11 0.15994025895005137 1108 12-13 0.23352935341683434 1108 14-15 0.1122281105834162 1108 16-17 0.21421219868255292 1108 18-19 0.2703225083634635 1108 20-21 0.19987353373356598 1108 22-23 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62-63 1.1794360192107085 2311 64-65 1.2042057084831193 2311 66-67 1.2336458350592068 2311 68-69 3.596202043099602 2311 70-71 5.210411089504841 2311 72-73 3.79317076266506 2311 74-75 2.986990098058019 2311 76-77 2.4806853338529713 2311 78-79 1.722342224753401 2311 80-81 1.4722789314972218 2311 82-83 1.3071662860639464 2311 84-85 1.4023222809451 2311 86-87 1.3911231047531238 2311 88-89 1.5669671710256843 2311 90-91 1.6815543833164845 2311 92-93 1.719373960668932 2311 94-95 1.8600100786599612 2311 96-97 1.794753660637781 2311 98-99 1.9278360598311153 2311 100-101 2.1772906955805844 2312 1 -0.15087251139192404 2312 2 -0.07486960337462989 2312 3 -0.09498648184391456 2312 4 0.009948613972639464 2312 5 -1.3144549641666075 2312 6 -0.26348967537565215 2312 7 -0.12662545906187006 2312 8 0.21639312071736327 2312 9 -2.628219255996285 2312 10-11 -0.0761089246490485 2312 12-13 -1.2875499410235918 2312 14-15 -4.128039518599287 2312 16-17 -1.069885280511599 2312 18-19 -0.03561805107908356 2312 20-21 0.12004867860272839 2312 22-23 0.23558797238107587 2312 24-25 0.3686476019102045 2312 26-27 0.4344063483349174 2312 28-29 0.44348900293660876 2312 30-31 0.12030446996046607 2312 32-33 0.4367296871337061 2312 34-35 0.13400653086316794 2312 36-37 0.4663177780839547 2312 38-39 0.3674142089405592 2312 40-41 0.008365853776311383 2312 42-43 -0.2853980685966846 2312 44-45 0.5155431624575542 2312 46-47 0.48443789531411596 2312 48-49 0.608474244221398 2312 50-51 0.5210564239523094 2312 52-53 0.5264242839898188 2312 54-55 0.4345756302275312 2312 56-57 0.40461307092691 2312 58-59 0.48852168221277736 2312 60-61 0.6708140558939739 2312 62-63 0.6724953985179098 2312 64-65 0.8062980653832348 2312 66-67 1.029500927244257 2312 68-69 3.3161278321173597 2312 70-71 4.238996872279898 2312 72-73 2.5160898916329444 2312 74-75 1.9205295340405257 2312 76-77 1.9621913328078442 2312 78-79 1.1318570827319618 2312 80-81 0.8329602626654875 2312 82-83 0.8236945377920861 2312 84-85 0.8418357711409001 2312 86-87 0.8726927582130877 2312 88-89 1.0783939437948824 2312 90-91 1.1573600599656295 2312 92-93 1.254554911313022 2312 94-95 1.4856353131996336 2312 96-97 1.6429729127061172 2312 98-99 1.8852994190354195 2312 100-101 1.855376360866611 2313 1 -0.16478109077077008 2313 2 -0.14597477554044502 2313 3 -0.10846358266019962 2313 4 -0.0029157579325271854 2313 5 0.09694737051399471 2313 6 0.10097835852035786 2313 7 -2.6582283285410746 2313 8 -3.1250186947507004 2313 9 -0.754200722964697 2313 10-11 0.039568790216996774 2313 12-13 0.08166399015704684 2313 14-15 -0.6098609158266726 2313 16-17 -0.20753758217087892 2313 18-19 0.17148211945768566 2313 20-21 -2.2955825862752093 2313 22-23 -0.49932091813197843 2313 24-25 0.1620530244509979 2313 26-27 -1.5981390963374906 2313 28-29 -1.320138895234443 2313 30-31 -0.6745083945582095 2313 32-33 -0.7380866409543714 2313 34-35 -1.198074560689527 2313 36-37 -2.034901442378107 2313 38-39 -2.036022649895507 2313 40-41 -1.0043847780315858 2313 42-43 -0.41142613231462377 2313 44-45 -0.4519546874877065 2313 46-47 -0.41422187943646094 2313 48-49 -2.1124688299606262 2313 50-51 -0.8862949287476596 2313 52-53 -0.8952304362976022 2313 54-55 -0.8441477802729516 2313 56-57 -0.7815042530841367 2313 58-59 -0.7324261617152636 2313 60-61 -0.32815347754561586 2313 62-63 -0.16612134578554816 2313 64-65 -0.02033597622068939 2313 66-67 0.41901049462095585 2313 68-69 1.208369730153393 2313 70-71 3.601398872344472 2313 72-73 2.8585440800569906 2313 74-75 1.4736659722583703 2313 76-77 1.3082158595756574 2313 78-79 0.765274583737277 2313 80-81 0.8168341182933432 2313 82-83 0.6925771550764708 2313 84-85 0.5637834272143287 2313 86-87 0.7600232854681508 2313 88-89 0.24318940240963016 2313 90-91 0.546656456385822 2313 92-93 0.6916577382314131 2313 94-95 0.8867206872504525 2313 96-97 1.0749713775817042 2313 98-99 1.2269677000776582 2313 100-101 1.158040594229 2314 1 -0.23017443980835495 2314 2 -0.23657026375164492 2314 3 -0.12393439185355248 2314 4 -0.11383976209203439 2314 5 -0.1892360721124362 2314 6 -2.7150792378803956 2314 7 -0.679315529576705 2314 8 -0.07784545988726421 2314 9 -0.0764816592775901 2314 10-11 -0.376508436497204 2314 12-13 -2.201530869884124 2314 14-15 -0.5538657119897437 2314 16-17 -1.8102608902500847 2314 18-19 -0.3998809670642487 2314 20-21 -0.9326955507708661 2314 22-23 -0.44878665527676986 2314 24-25 -0.381917816575303 2314 26-27 -2.0590073928507238 2314 28-29 -0.8025740090902644 2314 30-31 -0.2741709653864959 2314 32-33 -0.05805968906455661 2314 34-35 -0.102078614083986 2314 36-37 -0.46758909756369604 2314 38-39 -0.521114222655001 2314 40-41 -0.3480171287881504 2314 42-43 -0.4628421676279615 2314 44-45 -3.391501280301952 2314 46-47 -3.8565448352311194 2314 48-49 -3.473748832507262 2314 50-51 -3.7723356536898294 2314 52-53 -0.8087748220279565 2314 54-55 -1.2157549193404584 2314 56-57 -1.0946072220364087 2314 58-59 -1.6543813409347194 2314 60-61 -0.846580505834492 2314 62-63 -1.3989877959882158 2314 64-65 -1.2399254373951152 2314 66-67 -0.9548159309642408 2314 68-69 -1.3826257920129414 2314 70-71 1.358147986327623 2314 72-73 0.9599304046256556 2314 74-75 0.8313663606202475 2314 76-77 0.7506661471332272 2314 78-79 -1.699785049971684 2314 80-81 -1.102425871944554 2314 82-83 -0.011641770667889517 2314 84-85 0.1810554503038233 2314 86-87 -0.13618965568335284 2314 88-89 -0.2548507495396066 2314 90-91 0.18885632480967018 2314 92-93 -0.36631376067565924 2314 94-95 0.0568852635540118 2314 96-97 0.3866217033388004 2314 98-99 -0.13183463858238653 2314 100-101 -0.042576434272092456 2315 1 -0.48087011630904186 2315 2 -0.4071432717158956 2315 3 -0.49385227050943925 2315 4 -0.13616833954070984 2315 5 -0.387242708395263 2315 6 -0.1580266823449037 2315 7 -1.0227633511832224 2315 8 -2.7705639632178 2315 9 -0.8105225322639527 2315 10-11 -1.4515164928170776 2315 12-13 -0.7376741767644006 2315 14-15 -0.5339377408012638 2315 16-17 -0.45439783706420656 2315 18-19 -0.24257415053232023 2315 20-21 -0.9212925825039164 2315 22-23 -0.5141559888509093 2315 24-25 -5.222821788783651 2315 26-27 -1.108513389939823 2315 28-29 -1.0218983760051756 2315 30-31 -1.1276817502314103 2315 32-33 -0.5351119258884935 2315 34-35 -2.595676407183525 2315 36-37 -4.004660209194185 2315 38-39 -1.4271244143422948 2315 40-41 -0.24231520199973744 2315 42-43 -1.5642250216545293 2315 44-45 -5.263589053464315 2315 46-47 -3.4743020266032403 2315 48-49 -2.6343945067061476 2315 50-51 -1.3073593977007079 2315 52-53 -0.37824774394008287 2315 54-55 -1.5336847461384373 2315 56-57 -1.5592171367987753 2315 58-59 -0.9340528555582672 2315 60-61 -1.9505031368337917 2315 62-63 -0.8039894426645873 2315 64-65 -0.30141711371166124 2315 66-67 -0.2463718453653989 2315 68-69 2.6046721360078244 2315 70-71 3.141662099937669 2315 72-73 -2.7492368020800377 2315 74-75 -2.339105899080092 2315 76-77 -0.7255177777641677 2315 78-79 -0.7156944225702837 2315 80-81 -0.8906423974928259 2315 82-83 -1.3743762449885892 2315 84-85 -0.8263960258618646 2315 86-87 -0.5442948446616 2315 88-89 -0.5296118044040306 2315 90-91 -0.44409667940086806 2315 92-93 -1.3983332941527635 2315 94-95 -0.5020538873275804 2315 96-97 -0.793042374681793 2315 98-99 -2.954597371994332 2315 100-101 -4.505756219460503 2316 1 -0.6072894840561425 2316 2 -0.3779507947250984 2316 3 -0.9316866042429339 2316 4 -0.35884861164955595 2316 5 -0.24271689807113717 2316 6 -0.5804892673789226 2316 7 -0.6245544676298067 2316 8 -0.9698100616571779 2316 9 -5.311466910015053 2316 10-11 -1.6500571090635745 2316 12-13 -0.7529762976127401 2316 14-15 -0.5321990453230754 2316 16-17 -0.4449452560317937 2316 18-19 -0.5543116455303192 2316 20-21 -3.5068223944286885 2316 22-23 -1.3643736759257408 2316 24-25 -0.6078942177552378 2316 26-27 -0.6153525255940835 2316 28-29 -0.16082474655339496 2316 30-31 -0.053769785445496154 2316 32-33 -1.4603576241678056 2316 34-35 -0.7524030978597978 2316 36-37 -0.5031820179176734 2316 38-39 -5.672252865722847 2316 40-41 -5.149674945897292 2316 42-43 -3.7516179788374053 2316 44-45 -2.524194495641183 2316 46-47 -2.4807926228417365 2316 48-49 -2.740046767610508 2316 50-51 -1.1999256241913017 2316 52-53 -0.9461465034438845 2316 54-55 -0.35418854765904584 2316 56-57 -3.394672118791572 2316 58-59 -0.946390190492238 2316 60-61 -0.8415539571619242 2316 62-63 -0.4323299788790713 2316 64-65 -0.19241511291133762 2316 66-67 -0.13350029674595376 2316 68-69 2.418465653414785 2316 70-71 3.5461302872125806 2316 72-73 2.0930189002008746 2316 74-75 0.9293014638650838 2316 76-77 -1.171664236347599 2316 78-79 -0.07320982873274673 2316 80-81 -0.01189489849322456 2316 82-83 0.030426075939779196 2316 84-85 0.3106011730176874 2316 86-87 0.36402568354968423 2316 88-89 0.07526134485567582 2316 90-91 0.03101856669757197 2316 92-93 -0.009812285749404737 2316 94-95 0.18760797741832036 2316 96-97 0.3980936797399792 2316 98-99 0.350339402715548 2316 100-101 0.557786397586316 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 21.0 4 80.0 5 222.0 6 494.0 7 1238.0 8 2668.0 9 4374.0 10 7254.0 11 10263.0 12 12958.0 13 16289.0 14 20273.0 15 25690.0 16 30768.0 17 33686.0 18 29455.0 19 23971.0 20 23378.0 21 23913.0 22 24930.0 23 28165.0 24 33225.0 25 40150.0 26 50175.0 27 62139.0 28 75230.0 29 88813.0 30 103555.0 31 120225.0 32 138053.0 33 159422.0 34 183077.0 35 203783.0 36 200860.0 37 165068.0 38 70679.0 39 4069.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.73722284157067 22.584714480366657 16.232329988851728 20.44573268921095 2 15.50156719620028 23.033027557482853 39.12371782738941 22.34168741892745 3 18.14243025914785 28.27040903272341 31.839339428598606 21.747821279530132 4 11.959953174249408 19.551932504280213 38.76452210612746 29.72359221534292 5 11.530743017138928 40.33426779965134 34.55106233413801 13.583926849071718 6 27.40583794580558 39.90288419668675 17.979650464351653 14.711627393156022 7 25.51677605652572 36.039206963565725 21.6072259230435 16.836791056865057 8 25.900651979742605 34.79801309904138 19.938116169404758 19.36321875181126 9 26.271400129098033 17.194906820051905 21.946170182515687 34.587522868334375 10-11 23.62188145214129 28.451684260459974 27.323798872164158 20.602635415234577 12-13 25.271088530239204 26.79675877740299 28.04255974747117 19.889592944886633 14-15 22.715779732797785 27.497484668989376 27.662126768719837 22.124608829493006 16-17 19.544682413122278 30.757381429724273 28.608975568868328 21.088960588285126 18-19 19.222314281928103 28.580674644899297 32.6448670934439 19.5521439797287 20-21 20.56488067844335 27.341734778346204 32.583085185381506 19.510299357828934 22-23 20.263725114769173 27.41599818093279 32.473941317248396 19.84633538704965 24-25 19.923108000290306 27.966969285557347 32.7432302120512 19.36669250210114 26-27 19.862694061800553 27.62189642309015 33.140662789971856 19.37474672513745 28-29 19.22950502539262 27.704048130423164 33.454201021154034 19.612245823030186 30-31 19.51425158908302 27.925332046643813 33.0518122060797 19.508604158193457 32-33 19.577446019678927 28.04660741125311 32.607031783319776 19.768914785748184 34-35 19.926675469969812 27.79532741277757 32.72123872432855 19.556758392924067 36-37 20.123410421462207 27.331169064592142 32.72342108166334 19.82199943228231 38-39 19.7045238784148 28.135488498586884 32.568051814810104 19.59193580818821 40-41 19.451364091021205 28.088413375789074 32.98907661244842 19.471145920741296 42-43 19.451966907991483 28.17771336720012 32.206971811502875 20.163347913305522 44-45 19.703656584531945 28.239562646678735 31.93194379336528 20.12483697542404 46-47 20.131850719244333 28.584521001220637 31.123195195981314 20.160433083553713 48-49 19.918669360336132 28.590111919487377 30.955616424773496 20.535602295402995 50-51 19.988939473487207 28.844450129221254 30.662099675620684 20.504510721670847 52-53 20.02891336696728 29.298118474912833 30.36711499817092 20.305853159948963 54-55 20.09474743064011 29.03773813556823 30.279972313290042 20.587542120501624 56-57 20.38341153212733 29.422551232247322 29.631169443824344 20.562867791801008 58-59 20.29831222322009 28.86751107119631 30.00888173175188 20.825294973831717 60-61 20.379328428847927 29.177575913868537 30.185054027868713 20.258041629414826 62-63 20.413341196968453 28.91954198371177 30.393478169866906 20.27363864945287 64-65 20.30295854235881 29.015840522686716 30.27197504674277 20.409225888211708 66-67 20.042472326004873 29.152984722863113 30.005383466555525 20.799159484576492 68-69 20.388485169821912 28.779465427439728 30.231207310607246 20.600842092131117 70-71 20.11519058681219 28.851980470834338 30.675660646381893 20.35716829597158 72-73 20.49424356988161 28.740809499849853 29.86504855527426 20.899898374994283 74-75 20.006750857425807 29.538594755991447 29.62089871161224 20.833755674970504 76-77 19.445589552511073 29.332299551004574 30.38307442860486 20.83903646787949 78-79 19.885426214482127 29.16300443431516 29.968018430896525 20.98355092030619 80-81 19.7846283260765 29.518264780236574 29.681054337331076 21.016052556355852 82-83 20.040320363343987 29.526355864485136 29.437382559944275 20.995941212226597 84-85 19.724806773743225 29.183778208655625 29.414629216622462 21.67678580097869 86-87 19.681740280193196 29.34711502664136 29.62377106780711 21.34737362535833 88-89 20.072475434889764 28.878587546411772 29.492870111437792 21.55606690726067 90-91 19.73201449362956 28.83863699640632 29.90457964213774 21.52476886782638 92-93 19.887428621110836 29.180252177128885 29.666841132570227 21.265478069190056 94-95 19.648548401130473 28.742889623355477 29.745252250271374 21.863309725242676 96-97 19.826958401279388 28.497796032735028 29.540545489641516 22.134700076344068 98-99 19.65297370597793 28.65221244002213 29.29154702470857 22.403266829291375 100-101 19.501544188922416 28.491703839750176 29.751084226977813 22.255667744349598 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6830.0 1 4536.0 2 2309.0 3 2436.0 4 2232.0 5 2259.5 6 3679.5 7 6860.0 8 9817.0 9 10871.5 10 9532.0 11 7272.5 12 6210.0 13 5658.0 14 5366.0 15 5206.0 16 5087.5 17 5233.0 18 5426.0 19 5746.0 20 6415.0 21 7291.5 22 8228.5 23 9342.5 24 10611.0 25 12980.5 26 17061.0 27 20929.0 28 22624.0 29 25051.0 30 29944.5 31 35684.0 32 43986.5 33 53290.5 34 61505.0 35 68391.0 36 76149.0 37 82087.0 38 81231.0 39 78008.5 40 74291.5 41 70306.5 42 66360.0 43 64098.5 44 63484.0 45 61995.5 46 59853.0 47 60920.5 48 68623.0 49 65800.5 50 53199.0 51 45724.0 52 41718.5 53 40082.5 54 38765.0 55 36879.0 56 34654.0 57 32204.0 58 29688.5 59 27201.0 60 24081.0 61 20223.0 62 16197.0 63 12638.5 64 9984.0 65 8190.5 66 6726.0 67 5523.0 68 4371.5 69 3398.0 70 2674.5 71 2146.0 72 1725.0 73 1394.5 74 1100.0 75 788.0 76 558.0 77 390.5 78 253.5 79 162.5 80 99.5 81 64.0 82 48.5 83 34.0 84 19.5 85 7.5 86 4.0 87 2.5 88 1.5 89 1.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018378883406047992 2 0.018180728328893836 3 2.476938464426953E-4 4 0.0882780868721766 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.007604201085790746 12-13 0.0 14-15 0.0 16-17 3.9631015430831247E-4 18-19 1.733856925098867E-4 20-21 0.0 22-23 1.9815507715415624E-4 24-25 0.0037401770812846985 26-27 0.0031209424651779606 28-29 0.0011146223089921288 30-31 1.9815507715415624E-4 32-33 7.430815393280859E-4 34-35 0.0029970955419566132 36-37 0.08785700733322402 38-39 0.09796291626808598 40-41 0.08017849809350046 42-43 0.08416636902122786 44-45 0.08275451409650449 46-47 0.08139219794106968 48-49 0.07658693732008139 50-51 0.07619062716577307 52-53 0.06018960468557495 54-55 0.05184232206045613 56-57 0.02875725557199692 58-59 0.02147505648658168 60-61 0.013103004476818583 62-63 0.0023035527719170662 64-65 0.00629142369964446 66-67 0.15807821279972814 68-69 0.7274025188482632 70-71 0.8928620082719836 72-73 0.36188070965277785 74-75 0.054467876832748695 76-77 0.19877431177026295 78-79 0.013425006477194083 80-81 0.0031704812344664998 82-83 0.001882473232964484 84-85 1.4861630786561718E-4 86-87 0.002452169079782683 88-89 2.9723261573123437E-4 90-91 0.0039135627737945855 92-93 0.003938332158438855 94-95 0.07859325747626722 96-97 0.03876408696828181 98-99 0.013499314631126892 100-101 0.012260845398913417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2018621.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.63737653163145 #Duplication Level Percentage of deduplicated Percentage of total 1 70.8875240294472 24.553578612030357 2 12.571604214872941 8.708947775943983 3 5.382731658617884 5.593311096848423 4 2.9460954185642616 4.081800652436988 5 1.8040615083673643 3.124397887577169 6 1.2442166388906222 2.5857840124905245 7 0.8913502869741966 2.16118248580521 8 0.6345920284043312 1.7584482425450065 9 0.5096696831062717 1.5888258648458295 >10 2.654377262332052 16.696781530740097 >50 0.24922401697964033 6.0676738190851935 >100 0.20376707907721092 13.561962856292222 >500 0.015955584548586317 3.7908898669682047 >1k 0.0042450637789816816 2.72202593922526 >5k 1.4638150962005794E-4 0.42539577599560285 >10k+ 4.391445288601739E-4 2.578993581170004 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20222 1.001772992552837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17682 0.8759445185599476 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12961 0.6420719887487547 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8390 0.41563027433084265 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4212 0.20865729624332652 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3998 0.19805599961557913 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3503 0.1735343088177523 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3501 0.17343523127917523 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2946 0.14594121432403606 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2826 0.13999656200941138 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2214 0.10967883520482546 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2026 0.10036554657858011 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.953876928853906E-5 0.0 4 0.0 0.0 0.0 1.4861630786561718E-4 0.0 5 0.0 0.0 0.0 1.4861630786561718E-4 0.0 6 0.0 0.0 0.0 1.4861630786561718E-4 0.0 7 0.0 0.0 0.0 1.9815507715415624E-4 0.0 8 0.0 0.0 0.0 1.9815507715415624E-4 0.0 9 0.0 0.0 0.0 4.458489235968515E-4 0.0 10-11 0.0 0.0 0.0 4.458489235968515E-4 0.0 12-13 0.0 0.0 0.0 4.458489235968515E-4 0.0 14-15 0.0 0.0 0.0 4.458489235968515E-4 0.0 16-17 0.0 0.0 0.0 4.458489235968515E-4 0.0 18-19 0.0 0.0 0.0 4.458489235968515E-4 0.0 20-21 0.0 0.0 0.0 6.192346161067382E-4 0.0 22-23 0.0 0.0 0.0 8.916978471937031E-4 0.0 24-25 0.0 0.0 0.0 0.0014861630786561718 0.0 26-27 0.0 0.0 0.0 0.002452169079782683 0.0 28-29 0.0 0.0 0.0 0.005003415698142445 0.0 30-31 0.0 0.0 0.0 0.012880080015020154 0.0 32-33 0.0 0.0 0.0 0.025661082491463233 0.0 34-35 0.0 0.0 0.0 0.046715059439092335 0.0 36-37 0.0 0.0 0.0 0.07901433701521979 0.0 38-39 0.0 0.0 0.0 0.12449092722209866 0.0 40-41 0.0 0.0 0.0 0.17452508420352308 0.0 42-43 0.0 0.0 0.0 0.22542616964749698 0.0 44-45 0.0 0.0 0.0 0.2838571480233288 0.0 46-47 0.0 0.0 0.0 0.3598496201119477 0.0 48-49 0.0 4.953876928853906E-5 0.0 0.4434215239017131 0.0 50-51 0.0 9.907753857707812E-5 0.0 0.5362571775484353 0.0 52-53 0.0 1.4861630786561718E-4 0.0 0.6220087871868965 0.0 54-55 0.0 1.4861630786561718E-4 0.0 0.7158352162193894 0.0 56-57 0.0 1.4861630786561718E-4 0.0 0.8272231389646694 0.0 58-59 0.0 1.4861630786561718E-4 0.0 0.9456703363335663 0.0 60-61 0.0 1.4861630786561718E-4 0.0 1.0673623230908624 0.0 62-63 0.0 1.4861630786561718E-4 0.0 1.1815987250702336 2.476938464426953E-5 64-65 0.0 1.4861630786561718E-4 0.0 1.3001945387469962 4.953876928853906E-5 66-67 0.0 1.4861630786561718E-4 0.0 1.428945800127909 7.430815393280859E-5 68-69 0.0 1.4861630786561718E-4 0.0 1.5701808313695338 9.907753857707812E-5 70-71 0.0 1.733856925098867E-4 0.0 1.7538210491221482 9.907753857707812E-5 72-73 0.0 1.9815507715415624E-4 0.0 1.9556915339729448 9.907753857707812E-5 74-75 0.0 1.9815507715415624E-4 0.0 2.1707888702237814 9.907753857707812E-5 76-77 0.0 1.9815507715415624E-4 0.0 2.4055283284975237 9.907753857707812E-5 78-79 0.0 1.9815507715415624E-4 0.0 2.6785612554313065 9.907753857707812E-5 80-81 0.0 1.9815507715415624E-4 0.0 2.993206748567463 9.907753857707812E-5 82-83 0.0 1.9815507715415624E-4 0.0 3.311468571861682 9.907753857707812E-5 84-85 0.0 1.9815507715415624E-4 0.0 3.620714339145387 9.907753857707812E-5 86-87 0.0 1.9815507715415624E-4 0.0 3.9545808747654956 9.907753857707812E-5 88-89 2.476938464426953E-5 1.9815507715415624E-4 0.0 4.31504972949355 9.907753857707812E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 560 0.0 25.396742 9 GGTATCA 17305 0.0 25.24165 1 GTATCAA 23460 0.0 24.731173 1 GTACATG 13490 0.0 24.038664 1 TACATGG 13880 0.0 22.878653 2 GAGTACT 14485 0.0 22.69441 12-13 ACATGGG 13815 0.0 22.463665 3 CATGGGT 1410 0.0 22.21484 4 AGTACTT 15060 0.0 21.7335 12-13 GTACTTT 15495 0.0 21.03159 14-15 GTATACG 115 0.003733792 20.643044 1 CCTTTAG 760 0.0 20.615881 1 CATGGGA 9135 0.0 20.521399 4 TATCAAC 28360 0.0 20.252235 2 TTAGGCA 730 0.0 20.153812 4 ATCAACG 28510 0.0 20.1059 3 TCAACGC 28595 0.0 20.032518 4 CAACGCA 28745 0.0 19.8238 5 AACGCAG 29105 0.0 19.529736 6 AGTACAT 10310 0.0 19.428873 2 >>END_MODULE