FastQCFastQC Report
Fri 27 May 2016
SRR939463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916961
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81600.27974319848636986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66610.228354098666386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41800.14329982471483163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61600.051.87911
GTATCAA109100.042.3310131
TATCAAC140200.032.7243042
ATCAACG141700.032.0902183
TCAACGC144550.031.6326474
CAACGCA147000.030.8282435
AACGCAG151600.030.009976
ACGCAGA169450.026.5767387
CGCAGAG172100.026.243958
GTGGTAT32550.024.4680081
TGGTATC32700.024.1800862
GCAGAGT176150.023.8856989
TACATGG115900.023.7512362
GTACATG116350.023.6782051
GAGTACT100550.022.68362812-13
ACATGGG120050.022.391183
CAGAGTA173000.020.8311410-11
GTACTTT110150.020.68359814-15
AGAGTAC162850.020.04919410-11
AGTACTT107000.019.81605312-13
Produced by FastQC (version 0.11.5)