FastQCFastQC Report
Fri 27 May 2016
SRR939463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916961
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117010.4011366624373792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107790.3695284235887967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70650.24220412957183862No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31200.10696063471537672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73000.050.2578281
GTATCAA123500.039.379121
TATCAAC162000.029.659722
ATCAACG161600.029.5824643
TCAACGC165000.028.9728854
CAACGCA167450.028.4923885
AACGCAG173800.027.5059076
GTGGTAT37000.024.7252791
TGGTATC36350.024.6377752
ACGCAGA201700.023.6541927
CGCAGAG203000.023.4560348
GCAGAGT212400.021.6372389
GAGTACT122600.021.02363812-13
GTACATG130400.020.9741251
TACATGG130000.020.2663522
GTACTTT129550.019.84091814-15
AGTACTT129000.019.83368912-13
AGAGTAC199150.019.34170710-11
CAGAGTA210100.019.10037610-11
ACATGGG132250.019.0948433
Produced by FastQC (version 0.11.5)