##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939463_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2916961 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.968150071255668 31.0 31.0 31.0 30.0 34.0 2 31.23903610641349 31.0 31.0 34.0 30.0 34.0 3 31.233728527738286 31.0 31.0 34.0 28.0 34.0 4 35.16988125655434 35.0 35.0 37.0 33.0 37.0 5 34.788445920257416 35.0 35.0 37.0 32.0 37.0 6 34.74615601648428 35.0 35.0 37.0 32.0 37.0 7 34.61529310813549 35.0 35.0 37.0 30.0 37.0 8 34.624998757268266 35.0 35.0 37.0 30.0 37.0 9 35.974357901939726 37.0 35.0 39.0 30.0 39.0 10-11 35.837383667453906 37.0 35.0 39.0 30.0 39.0 12-13 35.80309935580215 37.0 35.0 39.0 30.0 39.0 14-15 36.67487772376799 38.0 36.0 40.0 31.0 41.0 16-17 36.6805596646647 38.0 35.5 40.0 31.0 41.0 18-19 36.621682291946996 38.0 35.0 40.0 30.0 41.0 20-21 36.53169617283193 38.0 35.0 40.0 30.0 41.0 22-23 36.39589884815052 38.0 35.0 40.0 30.0 41.0 24-25 36.179992293349144 38.0 34.0 40.0 29.5 41.0 26-27 35.80963732459912 38.0 34.0 40.0 27.5 41.0 28-29 35.517276885086915 38.0 34.0 40.0 27.0 41.0 30-31 35.304417165673456 38.0 34.0 40.0 27.0 41.0 32-33 35.114129739821685 37.5 33.0 40.0 27.0 41.0 34-35 35.08711943697568 38.0 33.0 40.0 26.5 41.0 36-37 35.110534388358296 38.0 33.5 40.0 26.5 41.0 38-39 35.07626310396334 38.0 33.0 40.0 26.0 41.0 40-41 34.85666572161918 38.0 33.0 40.0 25.5 41.0 42-43 34.568192375557985 38.0 33.0 40.0 24.5 41.0 44-45 34.38535671200266 38.0 33.0 40.0 24.0 41.0 46-47 34.08049096302625 37.0 33.0 40.0 24.0 41.0 48-49 33.78476726291507 37.0 32.5 40.0 23.0 41.0 50-51 33.52890508306419 37.0 32.0 40.0 23.0 41.0 52-53 33.16061956262014 37.0 31.0 40.0 21.0 41.0 54-55 32.93709240541783 36.5 31.0 39.0 20.5 40.5 56-57 32.54117213085811 36.0 31.0 39.0 19.0 40.0 58-59 32.1121207996953 36.0 30.0 39.0 17.5 40.0 60-61 31.829740952998687 35.0 30.0 39.0 16.0 40.0 62-63 31.442905818761375 35.0 29.0 38.5 13.0 40.0 64-65 31.001784391357994 34.5 29.0 38.0 10.0 40.0 66-67 29.298100660241943 33.5 25.5 37.5 7.0 39.5 68-69 25.604010303874475 30.0 16.0 35.0 4.5 39.0 70-71 25.000090333741177 29.5 15.0 35.0 2.0 38.0 72-73 26.840179213914755 31.0 22.0 35.0 3.5 38.0 74-75 27.447942910446866 32.0 24.5 35.0 2.0 38.0 76-77 27.514965918296475 31.5 25.5 34.5 2.0 36.5 78-79 28.358103519381988 33.0 26.5 35.0 2.0 37.0 80-81 28.21546619923955 33.0 26.0 35.0 2.0 37.0 82-83 27.802840181956498 33.0 26.0 35.0 2.0 36.0 84-85 27.458648744360996 32.5 26.0 35.0 2.0 36.0 86-87 27.008240254154924 32.0 25.5 35.0 2.0 35.5 88-89 26.513379849782016 32.0 25.0 34.0 2.0 35.0 90-91 26.06119125349979 32.0 24.0 34.0 2.0 35.0 92-93 25.481689333522112 31.5 21.5 34.0 2.0 35.0 94-95 24.088610543644567 31.0 15.0 34.0 2.0 35.0 96-97 23.272447934682706 30.0 4.5 34.0 2.0 35.0 98-99 22.927669756297735 30.0 2.0 34.0 2.0 35.0 100-101 21.656820745975004 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4050032524155718 1101 2 0.30678672081037206 1101 3 0.35704396389168735 1101 4 0.3112066128887321 1101 5 0.37347583880657 1101 6 0.5620058954372666 1101 7 0.6554744835611714 1101 8 0.527350246347396 1101 9 0.6490172234604188 1101 10-11 0.8032224899729314 1101 12-13 0.7468339354795219 1101 14-15 0.7676539517649488 1101 16-17 0.7804732295185843 1101 18-19 0.9128955846983828 1101 20-21 0.8742708088191975 1101 22-23 0.903609567497945 1101 24-25 0.7952242487939145 1101 26-27 0.8760966650006807 1101 28-29 0.8073318078071381 1101 30-31 0.8000610688574028 1101 32-33 0.8764382813854041 1101 34-35 0.9493044297777686 1101 36-37 0.8894555021636918 1101 38-39 0.9081915449598554 1101 40-41 1.0419889251879866 1101 42-43 1.1508924467471005 1101 44-45 0.6708052724132472 1101 46-47 0.7318931539897449 1101 48-49 0.8223788367253846 1101 50-51 0.702362823442165 1101 52-53 0.7877173013673016 1101 54-55 0.7154569945219365 1101 56-57 0.6535791504613258 1101 58-59 0.7395211193972706 1101 60-61 0.5796151729354229 1101 62-63 0.5094299054499771 1101 64-65 0.3473559606703951 1101 66-67 -2.8967824219058507 1101 68-69 -7.933340561187784 1101 70-71 -11.264743761717632 1101 72-73 -10.968183049838306 1101 74-75 -9.55382744321814 1101 76-77 -4.488916102922516 1101 78-79 -1.3120605127621268 1101 80-81 -0.6611697670766397 1101 82-83 -0.4356275050279059 1101 84-85 -0.5413148933605569 1101 86-87 -0.44205115747473656 1101 88-89 -0.4120851712043425 1101 90-91 -0.42856545334708684 1101 92-93 -1.12562438923473 1101 94-95 -4.963077976451061 1101 96-97 -1.7028919897491974 1101 98-99 -0.8907862609309198 1101 100-101 -0.596248208754286 1102 1 0.3691696738743744 1102 2 0.2794295333601653 1102 3 0.3942209180133851 1102 4 0.2134496930327643 1102 5 0.41583781734016156 1102 6 0.507372296784034 1102 7 0.46302599144718215 1102 8 0.5296992234759443 1102 9 0.7066584586054745 1102 10-11 0.6702532062748858 1102 12-13 0.6217466570822126 1102 14-15 0.47380492305348554 1102 16-17 0.5940212880561759 1102 18-19 0.7502749523216963 1102 20-21 0.806533121816635 1102 22-23 0.7225286674324494 1102 24-25 0.6468192226004987 1102 26-27 0.7046082096828243 1102 28-29 0.7135886085253844 1102 30-31 0.5268456907198527 1102 32-33 0.5979183550819087 1102 34-35 0.7261688772947821 1102 36-37 0.8924154642635145 1102 38-39 0.733734297163906 1102 40-41 0.6998472814811123 1102 42-43 0.9203141399658605 1102 44-45 0.7317352685520149 1102 46-47 0.6293687466794253 1102 48-49 0.6309962146975181 1102 50-51 0.589368772221917 1102 52-53 0.8438462739156378 1102 54-55 0.5930940084566529 1102 56-57 0.43865700785833894 1102 58-59 0.3059851087768841 1102 60-61 0.4173491550393855 1102 62-63 0.32896167922591957 1102 64-65 0.358863825449216 1102 66-67 -2.8119120639223105 1102 68-69 -7.819885448413238 1102 70-71 -11.020068552198813 1102 72-73 -9.357927139685426 1102 74-75 -7.588182440058986 1102 76-77 -3.8120613350442056 1102 78-79 -1.1644224266361185 1102 80-81 -0.6102512002460649 1102 82-83 -0.3990565175204317 1102 84-85 -0.3463071839095342 1102 86-87 -0.4038270073455692 1102 88-89 -0.24569729679026864 1102 90-91 -0.2675160492625146 1102 92-93 -0.635180643907475 1102 94-95 -4.218168110486552 1102 96-97 -1.0934314891893138 1102 98-99 -0.3675164287939978 1102 100-101 -0.20244861085979693 1103 1 0.3695572009655663 1103 2 0.32325901032128357 1103 3 0.4165093330620877 1103 4 0.2292946913980174 1103 5 0.42934605514243174 1103 6 0.4979856679160406 1103 7 0.5132017876533652 1103 8 0.45282517821549817 1103 9 0.6564711602348225 1103 10-11 0.6719729236398635 1103 12-13 0.5900004435228681 1103 14-15 0.6901045636396006 1103 16-17 0.6342585432026482 1103 18-19 0.699278476680739 1103 20-21 0.770224191315684 1103 22-23 0.6615012979362476 1103 24-25 0.6671540908657292 1103 26-27 0.5642708761203394 1103 28-29 0.8284162021029218 1103 30-31 0.8157612752804582 1103 32-33 0.7921932198140595 1103 34-35 0.8522195640746446 1103 36-37 0.8417362087334439 1103 38-39 0.7713136896560044 1103 40-41 0.8756689841292626 1103 42-43 0.8745540726245267 1103 44-45 0.8436689175494649 1103 46-47 0.6843410892457058 1103 48-49 0.6994431033093065 1103 50-51 0.805791006710983 1103 52-53 0.8444795159952676 1103 54-55 0.8181981246116479 1103 56-57 0.8851465424702241 1103 58-59 0.8509576133319818 1103 60-61 0.8090459725534167 1103 62-63 0.6509597413029091 1103 64-65 0.4621653396420733 1103 66-67 -2.837961542974906 1103 68-69 -7.850316772871182 1103 70-71 -11.661766400115084 1103 72-73 -9.257087472094145 1103 74-75 -7.625181548353687 1103 76-77 -3.842938751780757 1103 78-79 -0.7490063248135925 1103 80-81 -0.056338686284117756 1103 82-83 0.2086680581671203 1103 84-85 0.06903060734324384 1103 86-87 0.22023965191498363 1103 88-89 0.28308135833640335 1103 90-91 0.22906675352549755 1103 92-93 0.3992154287168077 1103 94-95 1.2156656884128658 1103 96-97 1.4215746659064479 1103 98-99 1.1083606088503792 1103 100-101 1.0752883076087407 1104 1 0.4063643170739972 1104 2 0.15688131691223361 1104 3 0.3727011902404591 1104 4 0.13159609738981004 1104 5 0.4435355018098761 1104 6 0.4539355984216016 1104 7 0.4690019177722249 1104 8 0.36245224187285885 1104 9 0.5683209608880091 1104 10-11 0.6834675812669886 1104 12-13 0.5949022662147954 1104 14-15 0.6918086620030621 1104 16-17 0.7169585876288309 1104 18-19 0.6409605865554227 1104 20-21 0.7884243672692719 1104 22-23 0.6164207083737026 1104 24-25 0.6658438609268913 1104 26-27 1.0046542718419502 1104 28-29 0.966274100352102 1104 30-31 0.7610103760022824 1104 32-33 0.5765351963949428 1104 34-35 0.7912873526516648 1104 36-37 0.7772806427259056 1104 38-39 0.7819181580092973 1104 40-41 0.9806424140708359 1104 42-43 0.9948194191697297 1104 44-45 0.8324035287479887 1104 46-47 0.7030907394894044 1104 48-49 0.6654373686383508 1104 50-51 0.5442051745155894 1104 52-53 0.6942654175518541 1104 54-55 0.609912773718996 1104 56-57 0.58675316589337 1104 58-59 0.6443588016087993 1104 60-61 0.5137020426668535 1104 62-63 0.49965954247001676 1104 64-65 0.3071898444728056 1104 66-67 -2.709057358500015 1104 68-69 -7.2275875716175015 1104 70-71 -8.776406142557398 1104 72-73 -8.198329031784553 1104 74-75 -6.985913680136914 1104 76-77 -3.596342998878683 1104 78-79 -0.7624965919760136 1104 80-81 0.024884101723781527 1104 82-83 0.21044748499695842 1104 84-85 0.22800812358050138 1104 86-87 0.2636547352721408 1104 88-89 0.2436064343881732 1104 90-91 0.1552033139944129 1104 92-93 0.2186589954659226 1104 94-95 1.057619951518685 1104 96-97 1.3908542611903414 1104 98-99 1.1923447109750356 1104 100-101 1.1519075888790482 1105 1 0.5085759846461002 1105 2 0.40324943024723225 1105 3 0.4604529370550914 1105 4 0.3180715180041318 1105 5 0.4956662437186239 1105 6 0.4922798608521788 1105 7 0.5167649656265709 1105 8 0.5099670650949975 1105 9 0.7593201786817332 1105 10-11 0.7716019779800689 1105 12-13 0.7916314642993214 1105 14-15 1.0252177927021435 1105 16-17 1.0093343870233582 1105 18-19 1.047792850272394 1105 20-21 1.0419352787086424 1105 22-23 1.1544091834171084 1105 24-25 1.0727746518268475 1105 26-27 1.1413556193256298 1105 28-29 1.111601695251906 1105 30-31 1.0363699015990662 1105 32-33 1.1167776803394318 1105 34-35 1.1607780018678469 1105 36-37 1.201971686276238 1105 38-39 1.093540289262151 1105 40-41 1.1628386178532253 1105 42-43 1.0659954937265397 1105 44-45 0.9217657112913429 1105 46-47 0.928608378203279 1105 48-49 0.9229027047278109 1105 50-51 0.8832358022868405 1105 52-53 1.1901732990502367 1105 54-55 1.1968306555855648 1105 56-57 1.2729290601144356 1105 58-59 1.4845114558127612 1105 60-61 1.4698250421611583 1105 62-63 1.397019013966073 1105 64-65 1.4393230622409838 1105 66-67 2.189588883641296 1105 68-69 -4.869162492476409 1105 70-71 -5.000238487425456 1105 72-73 -1.4320215150152116 1105 74-75 1.248447548129814 1105 76-77 1.3669414897680525 1105 78-79 1.2839745193655254 1105 80-81 1.2308599209552362 1105 82-83 1.4066702402114757 1105 84-85 1.3171775723016594 1105 86-87 1.4098531075918075 1105 88-89 1.365474040153206 1105 90-91 1.4260284289020895 1105 92-93 1.5433849602809175 1105 94-95 2.2410699583188425 1105 96-97 2.2768610343001825 1105 98-99 1.8498359515421612 1105 100-101 1.8738426514818478 1106 1 0.25188038406116675 1106 2 0.16808026680992683 1106 3 0.16330415530288533 1106 4 0.13918095796744012 1106 5 0.204665895905066 1106 6 0.29497243062576217 1106 7 0.30568857269012284 1106 8 0.19273655189466865 1106 9 0.40490176181154425 1106 10-11 0.455367756347961 1106 12-13 0.3555285114853959 1106 14-15 0.44191331746940676 1106 16-17 0.41835218806452446 1106 18-19 0.3649641783041844 1106 20-21 0.4716554262119175 1106 22-23 0.4855320525851923 1106 24-25 0.5239198303442976 1106 26-27 0.632607444872967 1106 28-29 0.6885055006355287 1106 30-31 0.6462549620115325 1106 32-33 0.5766890873921753 1106 34-35 0.7487942609671521 1106 36-37 0.6406943765146167 1106 38-39 0.6860184182336937 1106 40-41 0.686784918093096 1106 42-43 0.8121976918703311 1106 44-45 0.8166442276911781 1106 46-47 0.7035465157232608 1106 48-49 0.6169268033741844 1106 50-51 0.6240508232835893 1106 52-53 0.8522909529103515 1106 54-55 0.7916826831869841 1106 56-57 0.7467773186299453 1106 58-59 0.9110482562881366 1106 60-61 0.7452697364880088 1106 62-63 0.8074529469224103 1106 64-65 0.7142195948146259 1106 66-67 1.5527716336050439 1106 68-69 -5.275710532921348 1106 70-71 -6.376734251587013 1106 72-73 -2.435389004208819 1106 74-75 0.3927755808603024 1106 76-77 0.5892877528572633 1106 78-79 0.49384271034644556 1106 80-81 0.5271648046556656 1106 82-83 0.7196143066732503 1106 84-85 0.7175311669595459 1106 86-87 0.789034381006438 1106 88-89 0.6574427691552671 1106 90-91 0.5497020224606217 1106 92-93 0.6439371498478366 1106 94-95 1.7133831933356127 1106 96-97 1.7209692431740677 1106 98-99 1.5653642632816727 1106 100-101 1.4563290066705754 1107 1 -0.031778409405877284 1107 2 -0.0865809523167691 1107 3 0.044804759890148205 1107 4 -0.020605435821856588 1107 5 0.001989921047709231 1107 6 0.08295115968147826 1107 7 0.10288841497594348 1107 8 0.0653698766664661 1107 9 0.06399506124965626 1107 10-11 0.1837964191939392 1107 12-13 0.13977965912896906 1107 14-15 0.1553600594483342 1107 16-17 -0.008103682913969124 1107 18-19 0.2908983643143088 1107 20-21 0.1554334800599051 1107 22-23 0.11794684867040672 1107 24-25 0.28780708517696496 1107 26-27 0.21262692033198505 1107 28-29 0.2987622345581613 1107 30-31 0.2639199733941453 1107 32-33 0.1852701325459023 1107 34-35 0.3371695173867195 1107 36-37 0.30406808552221065 1107 38-39 0.16932415808425816 1107 40-41 0.16694928979752888 1107 42-43 0.33476982685534296 1107 44-45 0.13095542466673038 1107 46-47 0.06525330869123991 1107 48-49 0.19409060923758403 1107 50-51 0.16709173393189047 1107 52-53 0.23941136787994566 1107 54-55 0.24126478447983857 1107 56-57 0.29889956368833737 1107 58-59 0.21303308628970186 1107 60-61 0.17395314964505815 1107 62-63 0.2571180967191964 1107 64-65 0.30350312113987954 1107 66-67 1.0965148987076638 1107 68-69 -5.654541742198216 1107 70-71 -6.388104001169456 1107 72-73 -2.386507356612281 1107 74-75 0.21090881230000846 1107 76-77 0.22732650200253346 1107 78-79 0.35331874446846356 1107 80-81 0.3414762453181446 1107 82-83 0.3159649487639271 1107 84-85 0.5177484382215951 1107 86-87 0.6959056212217973 1107 88-89 0.587263079290743 1107 90-91 0.5059971136799497 1107 92-93 0.7474011594822407 1107 94-95 1.4489228355934607 1107 96-97 1.5706210876647688 1107 98-99 1.1312479476135273 1107 100-101 0.9261475037753293 1108 1 0.0562524352986884 1108 2 0.04160366626797796 1108 3 0.04363031280888663 1108 4 0.03302706106534714 1108 5 0.09634489203304497 1108 6 0.10838167786727837 1108 7 0.21892134289451093 1108 8 0.15124087262300634 1108 9 0.2892007385551878 1108 10-11 0.15578289941660017 1108 12-13 0.28340344272339024 1108 14-15 0.22916088690812586 1108 16-17 0.18667868319564462 1108 18-19 0.32850784497117047 1108 20-21 0.512684251441442 1108 22-23 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86-87 -4.984050293766266 2315 88-89 -3.406650490932364 2315 90-91 -3.3165218907070795 2315 92-93 -4.208211572702403 2315 94-95 -1.997049917964599 2315 96-97 -0.6606183528296441 2315 98-99 -2.580261380539728 2315 100-101 -1.6910832753181815 2316 1 -0.14137342769145533 2316 2 -0.08263632035996338 2316 3 -0.5877926769799977 2316 4 0.0027339802091717047 2316 5 0.172061923794665 2316 6 -2.1099290851458505 2316 7 -0.3064967577361841 2316 8 -0.09548998787480656 2316 9 0.4186888096658805 2316 10-11 0.36427617010821933 2316 12-13 -0.15194579778559358 2316 14-15 -0.4433912506807829 2316 16-17 -0.9843545469619315 2316 18-19 0.13846868394832512 2316 20-21 -4.0405207887493475 2316 22-23 -3.670756502111324 2316 24-25 -2.1834015623499425 2316 26-27 -1.25445566390988 2316 28-29 -0.7397861123925153 2316 30-31 0.34122471354999817 2316 32-33 -1.1128567764190578 2316 34-35 -1.5786853315529612 2316 36-37 -3.84246563896307 2316 38-39 -1.6512584389027865 2316 40-41 -1.2355122535083254 2316 42-43 -2.282456552959893 2316 44-45 -0.16423878182611418 2316 46-47 -0.3988523398207917 2316 48-49 0.08951287940831776 2316 50-51 0.3927308295342016 2316 52-53 -1.756054348346467 2316 54-55 -0.10202652975772253 2316 56-57 0.7334568337616219 2316 58-59 0.9911078974736647 2316 60-61 -0.7184689435926508 2316 62-63 -0.07337481171496307 2316 64-65 0.7695664661637842 2316 66-67 1.9115805213694834 2316 68-69 4.546604311520451 2316 70-71 4.7234854497233805 2316 72-73 2.7866804667965575 2316 74-75 1.7118352741342058 2316 76-77 0.4712326210364779 2316 78-79 0.8737475746562389 2316 80-81 0.4295229196519088 2316 82-83 0.7165094676635206 2316 84-85 0.8325426636072848 2316 86-87 0.4737112842783304 2316 88-89 0.8923248634054559 2316 90-91 1.0913954154227419 2316 92-93 1.2288426838652633 2316 94-95 2.0938930268760636 2316 96-97 2.415056468872983 2316 98-99 1.225236497947229 2316 100-101 0.7848014407601802 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 26.0 4 83.0 5 184.0 6 451.0 7 1147.0 8 2529.0 9 4288.0 10 6778.0 11 10473.0 12 13997.0 13 17745.0 14 21490.0 15 26798.0 16 31412.0 17 36148.0 18 33875.0 19 29642.0 20 29329.0 21 29857.0 22 31676.0 23 35993.0 24 43418.0 25 55048.0 26 68754.0 27 86708.0 28 105004.0 29 126138.0 30 148673.0 31 173690.0 32 199724.0 33 231984.0 34 269061.0 35 308848.0 36 331213.0 37 281169.0 38 116811.0 39 6789.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.047259878927036 20.480149561527135 15.517077021306072 22.955513538239757 2 15.96000646008344 21.196342989283597 39.18839544077211 23.65525510986085 3 19.343076578672118 24.82264932578804 30.355702390261648 25.478571705278195 4 13.36298291269578 17.75087154062053 36.43075790180259 32.45538764488109 5 13.543390065952268 37.813605818115995 34.0520158329328 14.590988282998932 6 31.650714562176184 36.73460152535464 17.126214577431785 14.48846933503739 7 28.019298166824996 33.139627166767056 20.424784561740797 18.41629010466715 8 26.125512134032647 35.658412985295314 19.252639990730078 18.96343488994196 9 25.88810752012111 17.047536802857493 20.35423168153431 36.71012399548708 10-11 24.88540299304653 27.20308910540799 26.95702822218055 20.95447967936493 12-13 25.689921805605216 24.44273337902015 27.715848789202184 22.151496026172445 14-15 22.612215247307045 25.846848826569847 26.29632346815744 25.24461245796567 16-17 21.7749532902518 29.131352954284438 26.931880902997996 22.161812852465758 18-19 21.58851982037236 27.29384697504871 29.721662176204173 21.395971028374763 20-21 22.51685915581319 26.364270897005483 29.83977159790618 21.279098349275152 22-23 22.09757003950344 26.467717600612417 29.651064926819387 21.783647433064754 24-25 21.92439322980321 26.594493378553913 29.805746460100085 21.675366931542793 26-27 21.830803035842752 26.85864119347969 29.663626332920746 21.646929437756807 28-29 21.5968931964266 26.90434358625145 29.81878922164641 21.679973995675546 30-31 21.825546613183853 26.652267448338517 29.74516811944494 21.777017819032682 32-33 21.645310027971714 26.78408603706282 29.597560116385097 21.973043818580372 34-35 22.216218430881323 26.79497599235842 29.403082379236224 21.585723197524032 36-37 22.01506315378391 26.617434191787364 29.436310525950944 21.931192128477782 38-39 21.838144010597418 26.850296894129638 29.581225274909123 21.730333820363825 40-41 22.094611883555714 26.689373500414533 29.52296256736068 21.693052048669074 42-43 21.99856894542946 26.78187742691584 29.21695233421473 22.002601293439973 44-45 22.093966954088565 26.991503627061427 29.034078488929733 21.88045092992028 46-47 22.082280092600538 27.133820540819524 28.61057672179551 22.173322644784424 48-49 22.231447926532965 27.19112479237876 28.477449930678528 22.099977350409752 50-51 22.221122173936394 27.354729139037964 28.314659489394955 22.10948919763069 52-53 22.493184951604622 27.369141721613914 28.09551411908541 22.042159207696052 54-55 22.113826716225653 27.29561636269766 28.33608057508546 22.25447634599123 56-57 22.21270918838465 27.682082525616174 28.066923135620176 22.038285150379004 58-59 22.25451933354992 27.30244068441684 28.194520996503513 22.248518985529735 60-61 22.30573528148758 27.225954453126032 28.407937094866664 22.060373170519725 62-63 22.260757982190434 27.243727149449477 28.339867918696992 22.155646949663094 64-65 22.102779571484085 27.21354262643287 28.3184561894067 22.365221612676343 66-67 21.651033231979376 27.379614634630002 28.729250669266616 22.240101464124002 68-69 21.740949450748467 26.934261071046965 29.2669751099635 22.057814368241065 70-71 21.23836773070732 26.920411945178678 29.569115250488366 22.272105073625635 72-73 22.08993738118988 27.165277114319508 28.322132515215515 22.422652989275097 74-75 21.844201952535375 27.399680259500332 28.226034182673004 22.53008360529129 76-77 22.000840033601342 27.412662220774546 28.016372083454765 22.570125662169342 78-79 21.985368195908535 27.55890717973922 27.878683257629223 22.577041366723023 80-81 21.864653586099735 27.56897037888391 27.92912735940019 22.637248675616167 82-83 22.061607954305867 27.611665017118842 27.749753939116772 22.576973089458516 84-85 21.92857566487862 27.494419705988527 27.70474133867405 22.872263290458804 86-87 21.961442332596434 27.405628916862117 27.918063885444944 22.7148648650965 88-89 21.901583424396307 27.415877097126696 27.733796147500588 22.94874333097641 90-91 21.775612294731125 27.594064264998625 27.896271198410766 22.73405224185948 92-93 21.930635620167198 27.38252571638186 27.87081557055999 22.816023092890948 94-95 21.40967137766572 27.261604241033005 28.170698898407554 23.158025482893724 96-97 21.390182126936665 27.101136235951678 28.237337826001653 23.271343811110007 98-99 21.623897996357012 27.06038358512804 27.742333654773383 23.57338476374156 100-101 21.503543405148836 27.06226933924721 27.953773309496356 23.480413946107603 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1765.0 1 1193.5 2 658.5 3 732.0 4 718.5 5 735.5 6 1108.0 7 2107.0 8 3133.5 9 3767.5 10 3797.5 11 3440.0 12 3392.5 13 3453.5 14 3568.5 15 3815.0 16 4144.0 17 4482.0 18 4842.0 19 5209.0 20 5805.0 21 6711.5 22 7839.5 23 9363.0 24 11236.5 25 13862.0 26 17812.0 27 22417.0 28 26400.0 29 30779.5 30 35874.5 31 41437.5 32 47465.0 33 53416.0 34 60082.5 35 66345.5 36 72566.0 37 78613.0 38 82054.0 39 84246.0 40 86918.0 41 90977.5 42 94782.0 43 97715.0 44 101464.5 45 104054.5 46 105538.0 47 108106.0 48 112749.5 49 114255.0 50 111560.0 51 108423.5 52 104300.0 53 100851.5 54 96516.0 55 89060.5 56 80579.5 57 71596.5 58 62922.0 59 54443.0 60 45447.0 61 36321.5 62 28090.0 63 21456.5 64 15909.0 65 11803.5 66 8760.5 67 6519.5 68 4651.5 69 3198.5 70 2292.5 71 1634.0 72 1260.5 73 1038.5 74 776.0 75 571.0 76 395.0 77 219.5 78 123.0 79 69.5 80 41.0 81 26.0 82 16.5 83 8.5 84 5.0 85 2.5 86 2.5 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02410042506567623 2 0.020226530282715472 3 0.0 4 0.0 5 1.028467641494007E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.771048018811359E-4 18-19 0.001302725679225742 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.9424592923936936E-4 28-29 0.017929619216712187 30-31 0.004662386641439498 32-33 0.009067656372505494 34-35 0.009787583721551299 36-37 0.10157832072489141 38-39 0.104338042229567 40-41 0.11818807313501963 42-43 0.10373810277202883 44-45 0.09921284514945522 46-47 0.10075554661169621 48-49 0.10288104640411716 50-51 0.10104694577678618 52-53 0.07787214158845455 54-55 0.06568480003675058 56-57 0.03697341171170955 58-59 0.015889825061082406 60-61 0.008621987061191425 62-63 0.0015598425895992439 64-65 0.004336705221633062 66-67 0.4212946282106617 68-69 0.8095583040020076 70-71 0.8977836865148352 72-73 0.28010316216089276 74-75 0.14695088484213537 76-77 0.014090006688467896 78-79 0.007970624221578553 80-81 0.0013198668065839757 82-83 0.0 84-85 0.0 86-87 1.8855240094056794E-4 88-89 8.056329858369721E-4 90-91 0.00293113277825792 92-93 0.0015769837169574774 94-95 0.22413738133626057 96-97 0.23099383227955395 98-99 0.013661478504512058 100-101 0.00701072108951748 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2916961.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.239423388738096 #Duplication Level Percentage of deduplicated Percentage of total 1 74.63584670670495 41.228411362086284 2 14.348204111062158 15.8517304351799 3 4.913498421140644 8.142564588158526 4 2.1752294343557397 4.806336787680879 5 1.161677777780677 3.208520530405761 6 0.6666518647394694 2.2095278765541186 7 0.436770524374867 1.68888663437651 8 0.29651318188930964 1.3103373755780388 9 0.20136925070669753 1.001116916753419 >10 1.0324017439165583 10.33881579803015 >50 0.0810757822202696 3.067387883816299 >100 0.047315506592212016 4.690966704903478 >500 0.0028192046044963736 1.0188283578528832 >1k 4.3854293843388094E-4 0.41410666199396506 >5k 6.264899120484013E-5 0.24449804170043146 >10k+ 1.2529798240968026E-4 0.7779640449293276 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11701 0.4011366624373792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10779 0.3695284235887967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7065 0.24220412957183862 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3120 0.10696063471537672 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.42822547164669E-5 0.0 4 0.0 0.0 0.0 3.42822547164669E-5 0.0 5 6.85645094329338E-5 0.0 0.0 3.42822547164669E-5 0.0 6 6.85645094329338E-5 0.0 0.0 3.42822547164669E-5 0.0 7 1.028467641494007E-4 0.0 0.0 3.42822547164669E-5 0.0 8 1.371290188658676E-4 0.0 0.0 3.42822547164669E-5 0.0 9 1.371290188658676E-4 0.0 0.0 3.42822547164669E-5 0.0 10-11 1.371290188658676E-4 0.0 0.0 3.42822547164669E-5 0.0 12-13 1.371290188658676E-4 0.0 0.0 6.85645094329338E-5 0.0 14-15 2.056935282988014E-4 0.0 0.0 6.85645094329338E-5 0.0 16-17 2.3997578301526828E-4 0.0 0.0 6.85645094329338E-5 0.0 18-19 2.742580377317352E-4 0.0 0.0 1.028467641494007E-4 0.0 20-21 3.085402924482021E-4 0.0 0.0 1.5427014622410105E-4 0.0 22-23 3.42822547164669E-4 0.0 0.0 2.742580377317352E-4 3.42822547164669E-5 24-25 4.456693113140697E-4 0.0 0.0 3.256814198064356E-4 1.028467641494007E-4 26-27 4.6281043867230315E-4 0.0 0.0 7.199273490458048E-4 1.028467641494007E-4 28-29 5.827983301799373E-4 0.0 0.0 0.001748394990539812 1.028467641494007E-4 30-31 5.827983301799373E-4 0.0 0.0 0.004216717330125429 1.028467641494007E-4 32-33 5.827983301799373E-4 0.0 0.0 0.0099932772498501 1.028467641494007E-4 34-35 6.685039669711045E-4 0.0 0.0 0.019506602933669667 1.714112735823345E-4 36-37 7.199273490458048E-4 0.0 0.0 0.035807815051349674 1.714112735823345E-4 38-39 7.199273490458048E-4 0.0 0.0 0.05992538124438414 1.714112735823345E-4 40-41 7.542096037622718E-4 0.0 0.0 0.08618558835719778 1.714112735823345E-4 42-43 8.741974952699059E-4 0.0 0.0 0.11385136791338657 1.8855240094056794E-4 44-45 9.599031320610731E-4 0.0 0.0 0.15044767482321497 2.056935282988014E-4 46-47 0.0010113265141357733 0.0 0.0 0.19336905772823154 2.056935282988014E-4 48-49 0.0010627498962104739 0.0 0.0 0.24117566193034462 2.3997578301526828E-4 50-51 0.0010627498962104739 0.0 0.0 0.29049068533998224 2.5711691037350173E-4 52-53 0.0010627498962104739 0.0 0.0 0.34337106324013245 2.913991650899686E-4 54-55 0.0010627498962104739 0.0 0.0 0.40204514218736553 3.085402924482021E-4 56-57 0.0010970321509269408 1.714112735823345E-5 0.0 0.4712781555872705 3.085402924482021E-4 58-59 0.0010970321509269408 3.42822547164669E-5 0.0 0.5409911205532059 3.085402924482021E-4 60-61 0.0010970321509269408 3.42822547164669E-5 0.0 0.612812444184204 3.085402924482021E-4 62-63 0.0010970321509269408 3.42822547164669E-5 0.0 0.6862278926595179 3.085402924482021E-4 64-65 0.0010970321509269408 5.1423382074700354E-5 0.0 0.7624544860215821 3.085402924482021E-4 66-67 0.0011655966603598745 6.85645094329338E-5 0.0 0.8449033086146849 3.085402924482021E-4 68-69 0.0011998789150763414 6.85645094329338E-5 0.0 0.9474929558537122 3.085402924482021E-4 70-71 0.0011998789150763414 6.85645094329338E-5 0.0 1.079342507493244 3.771048018811359E-4 72-73 0.0012513022971510418 6.85645094329338E-5 0.0 1.2232251305382555 3.771048018811359E-4 74-75 0.001302725679225742 6.85645094329338E-5 0.0 1.3816777118377654 3.771048018811359E-4 76-77 0.001302725679225742 6.85645094329338E-5 0.0 1.5673332622547917 3.771048018811359E-4 78-79 0.001302725679225742 6.85645094329338E-5 0.0 1.7782548343978544 3.771048018811359E-4 80-81 0.0014227135707333763 8.570563679116725E-5 0.0 2.0125569042575475 3.9424592923936936E-4 82-83 0.0014741369528080766 1.028467641494007E-4 0.0 2.255909489362388 4.113870565976028E-4 84-85 0.0015598425895992439 1.028467641494007E-4 0.0 2.497068010165374 4.113870565976028E-4 86-87 0.0016284070990321775 1.028467641494007E-4 0.0 2.7573388879727907 4.113870565976028E-4 88-89 0.0016455482263904111 1.028467641494007E-4 0.0 3.0360707599450247 4.113870565976028E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7300 0.0 50.257828 1 GTATCAA 12350 0.0 39.37912 1 TATCAAC 16200 0.0 29.65972 2 ATCAACG 16160 0.0 29.582464 3 TCAACGC 16500 0.0 28.972885 4 CAACGCA 16745 0.0 28.492388 5 AACGCAG 17380 0.0 27.505907 6 GTGGTAT 3700 0.0 24.725279 1 TGGTATC 3635 0.0 24.637775 2 ACGCAGA 20170 0.0 23.654192 7 CGCAGAG 20300 0.0 23.456034 8 GCAGAGT 21240 0.0 21.637238 9 GAGTACT 12260 0.0 21.023638 12-13 GTACATG 13040 0.0 20.974125 1 TACATGG 13000 0.0 20.266352 2 GTACTTT 12955 0.0 19.840918 14-15 AGTACTT 12900 0.0 19.833689 12-13 AGAGTAC 19915 0.0 19.341707 10-11 CAGAGTA 21010 0.0 19.100376 10-11 ACATGGG 13225 0.0 19.094843 3 >>END_MODULE