FastQCFastQC Report
Fri 27 May 2016
SRR939462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908034
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82890.2850379328439764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63150.21715702086014124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.14294881008956567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65750.046.7858281
GTATCAA113500.041.3861161
TATCAAC144000.032.3892332
ATCAACG147100.032.0031853
TCAACGC150100.031.6767794
CAACGCA151700.031.1036365
AACGCAG156250.030.142316
ACGCAGA175150.026.5855797
CGCAGAG177700.026.0685148
GTGGTAT36100.024.5456391
GCAGAGT178000.024.0339939
GTACATG116300.023.8528771
TACATGG113350.023.0994952
GAGTACT99050.022.71706612-13
TGGTATC37350.022.1445242
ACATGGG118250.021.6152553
GTACTTT109900.020.33818214-15
CAGAGTA174150.020.06689510-11
AGTACTT104900.019.77726412-13
AGAGTAC164000.019.75614710-11
Produced by FastQC (version 0.11.5)