FastQCFastQC Report
Fri 27 May 2016
SRR939462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908034
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115320.39655657396027694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103580.35618565670140034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69780.2399559289884506No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29890.10278421779112625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73000.049.553991
GTATCAA121500.038.1346781
ATCAACG158800.028.7150863
TATCAAC159500.028.6532
TCAACGC161050.028.2588794
CAACGCA163300.027.8450765
AACGCAG169150.026.9941866
GTGGTAT37650.025.8485051
TGGTATC37500.025.4371992
GTATACG1652.4440578E-623.0172041
ACGCAGA197650.022.8858767
CGCAGAG198350.022.7812048
TACATGG135700.022.1374872
GTACATG139150.022.1074331
ACATGGG135450.021.2640933
GCAGAGT209100.020.8163539
GAGTACT115800.020.57198712-13
AGTACTT122700.019.43444612-13
GTACTTT123750.019.28870214-15
AGAGTAC195300.019.05679110-11
Produced by FastQC (version 0.11.5)