##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939462_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2908034 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.866532853467326 31.0 31.0 31.0 28.0 34.0 2 31.124322480411163 31.0 31.0 34.0 28.0 34.0 3 31.129907353215266 31.0 31.0 34.0 28.0 34.0 4 35.04613494890362 35.0 35.0 37.0 32.0 37.0 5 34.60778106445798 35.0 35.0 37.0 30.0 37.0 6 34.66377765872063 35.0 35.0 37.0 31.0 37.0 7 34.507302184224805 35.0 35.0 37.0 30.0 37.0 8 34.40231166485674 35.0 35.0 37.0 30.0 37.0 9 35.90491582973239 37.0 35.0 39.0 30.0 39.0 10-11 35.70841400066161 37.0 35.0 39.0 30.0 39.0 12-13 35.69027752082678 37.0 35.0 39.0 30.0 39.0 14-15 36.53307148403354 38.0 35.0 40.0 30.5 41.0 16-17 36.43697219496058 38.0 35.0 40.0 30.0 41.0 18-19 36.4251948223439 38.0 35.0 40.0 30.0 41.0 20-21 36.30387333848229 38.0 34.5 40.0 29.5 41.0 22-23 36.1432197835376 38.0 34.0 40.0 29.0 41.0 24-25 35.98274710680824 38.0 34.0 40.0 29.0 41.0 26-27 35.523109427193766 38.0 34.0 40.0 27.0 41.0 28-29 35.30278910081519 38.0 34.0 40.0 27.0 41.0 30-31 35.01209717630537 37.5 33.0 40.0 27.0 41.0 32-33 34.78665844347074 37.0 33.0 40.0 25.5 41.0 34-35 35.054415457315834 38.0 33.0 40.0 27.0 41.0 36-37 34.909134831298395 38.0 33.0 40.0 25.5 41.0 38-39 34.72774664945458 38.0 33.0 40.0 25.0 41.0 40-41 34.570949995770334 37.5 33.0 40.0 25.0 41.0 42-43 34.41219428658675 37.0 33.0 40.0 24.5 41.0 44-45 34.02494537546673 37.0 33.0 40.0 24.0 41.0 46-47 33.64171481488869 37.0 32.0 40.0 23.0 41.0 48-49 33.2629940021334 37.0 31.5 40.0 21.5 41.0 50-51 33.017347802673555 37.0 31.0 39.0 20.5 40.0 52-53 32.90864618501709 36.5 31.0 39.0 20.5 40.0 54-55 32.5685053888641 36.0 31.0 39.0 19.0 40.0 56-57 32.069647741395045 35.5 30.0 39.0 16.5 40.0 58-59 31.607317864921797 35.0 29.5 39.0 14.0 40.0 60-61 31.238907110439563 35.0 29.0 38.0 13.0 40.0 62-63 31.078419647088033 35.0 29.0 38.0 12.0 40.0 64-65 30.62001647848684 34.0 28.0 38.0 8.0 40.0 66-67 29.746133470241407 34.0 27.0 37.5 7.5 39.5 68-69 26.322081516240868 31.0 19.0 36.0 2.0 39.0 70-71 25.008194367741233 30.0 13.5 35.0 2.0 38.0 72-73 25.992180627874365 31.0 19.5 35.0 2.0 38.0 74-75 26.72604360884364 31.0 22.5 35.0 2.0 37.5 76-77 26.409776501925357 30.5 23.0 34.0 2.0 36.0 78-79 27.522998527527534 32.0 26.0 35.0 2.0 37.0 80-81 27.536743552516924 32.0 26.0 35.0 2.0 36.5 82-83 27.204453421108557 32.0 25.5 35.0 2.0 36.0 84-85 26.834894984033888 32.0 25.0 34.5 2.0 36.0 86-87 26.378491792049196 32.0 24.5 34.0 2.0 35.0 88-89 25.894448964489413 31.5 23.5 34.0 2.0 35.0 90-91 25.297995312296898 31.0 20.5 34.0 2.0 35.0 92-93 24.717785280364673 31.0 19.0 34.0 2.0 35.0 94-95 23.86711933216737 31.0 13.0 34.0 2.0 35.0 96-97 23.185167023494223 30.0 4.5 34.0 2.0 35.0 98-99 22.310025261052658 30.0 2.0 34.0 2.0 35.0 100-101 20.877752632878433 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2770487511785902 1101 2 0.20878542880517514 1101 3 0.3138520214371674 1101 4 0.30096747915675337 1101 5 0.47203800799864126 1101 6 0.4063817840744335 1101 7 0.5442284778764517 1101 8 0.6321058892062794 1101 9 0.6163837564878634 1101 10-11 0.7944903806670212 1101 12-13 0.7317271862532877 1101 14-15 0.7582612431693789 1101 16-17 0.6457023939790574 1101 18-19 0.5247591681314958 1101 20-21 0.7340106905984598 1101 22-23 0.791245890214924 1101 24-25 0.7348615404712646 1101 26-27 0.7579987305088096 1101 28-29 0.621464425039072 1101 30-31 0.4716830451552809 1101 32-33 0.904108459381554 1101 34-35 0.7299638315693784 1101 36-37 0.8705857184653567 1101 38-39 0.9965372704974342 1101 40-41 0.8254519288479116 1101 42-43 0.5560540644599996 1101 44-45 0.7913574752435792 1101 46-47 0.7445585985159013 1101 48-49 0.8733334364162602 1101 50-51 0.5498261500000297 1101 52-53 0.27000146803856495 1101 54-55 0.5448794485807582 1101 56-57 0.6562815002082267 1101 58-59 0.7573046598017434 1101 60-61 0.7179185188509578 1101 62-63 0.399936525693807 1101 64-65 0.28671944726703913 1101 66-67 -0.005435297179669618 1101 68-69 -6.978673002149765 1101 70-71 -7.7126913972428355 1101 72-73 -5.095604062583217 1101 74-75 -2.943666861893945 1101 76-77 -0.1055961437097217 1101 78-79 0.2523795184891462 1101 80-81 0.08030936284599832 1101 82-83 -0.166936791593983 1101 84-85 -0.19707072474535892 1101 86-87 -0.1465537726006474 1101 88-89 -0.19039267617283784 1101 90-91 -0.3228679203617091 1101 92-93 -0.22793709832950526 1101 94-95 -0.25658795260283895 1101 96-97 -0.4754981721161471 1101 98-99 -0.43068599687672204 1101 100-101 -0.5151446446034207 1102 1 0.3323877250820608 1102 2 0.3022262911599505 1102 3 0.34730408774673194 1102 4 0.3407246343411785 1102 5 0.4726473511803633 1102 6 0.38110414247676516 1102 7 0.3873664776750445 1102 8 0.4562007839295035 1102 9 0.5710062320553391 1102 10-11 0.6262928184015522 1102 12-13 0.5915773499407777 1102 14-15 0.6909514345230363 1102 16-17 0.6826502690567651 1102 18-19 0.46258204313383544 1102 20-21 0.6388274263401001 1102 22-23 0.6067495284851674 1102 24-25 0.6941947423480812 1102 26-27 0.7314983322196795 1102 28-29 0.5442920608176749 1102 30-31 0.4597343710872579 1102 32-33 0.7655344430290896 1102 34-35 0.4969188583641966 1102 36-37 0.6297876748760629 1102 38-39 0.7902580206679275 1102 40-41 0.6777804278375683 1102 42-43 0.624043165629061 1102 44-45 0.6339183929789343 1102 46-47 0.6339213539892867 1102 48-49 0.7171991204152945 1102 50-51 0.6359384621091664 1102 52-53 0.3371590162659217 1102 54-55 0.27064682601714907 1102 56-57 0.406275470092627 1102 58-59 0.513093420028067 1102 60-61 0.48871834735447095 1102 62-63 0.2372678256721592 1102 64-65 0.0327272092318438 1102 66-67 -0.030962133304644368 1102 68-69 -6.9194225937601885 1102 70-71 -7.643896974134954 1102 72-73 -1.5988478125191428 1102 74-75 0.08846717189761577 1102 76-77 0.4306020372456061 1102 78-79 0.10427671346026912 1102 80-81 -0.16192430051556173 1102 82-83 -0.3825717657820711 1102 84-85 -0.5097062892817661 1102 86-87 -0.3230101811964552 1102 88-89 -0.5496654562913079 1102 90-91 -0.5169848792779312 1102 92-93 -0.43000612144474815 1102 94-95 -0.23539842787846155 1102 96-97 -0.46834479672878615 1102 98-99 -0.5271224699103492 1102 100-101 -0.5409155496574449 1103 1 0.4262116857062104 1103 2 0.3602806540368064 1103 3 0.3718630875256501 1103 4 0.39667551925059286 1103 5 0.5701488347563028 1103 6 0.5292467635772837 1103 7 0.47614715278936615 1103 8 0.5599417224803673 1103 9 0.5192006328651999 1103 10-11 0.582531837030615 1103 12-13 0.5177784646590098 1103 14-15 0.6361951191438777 1103 16-17 0.6383598547027347 1103 18-19 0.7121605330280616 1103 20-21 0.5557894991574983 1103 22-23 0.6273558165199802 1103 24-25 0.675637825393423 1103 26-27 0.928769861524664 1103 28-29 0.6627362550904934 1103 30-31 0.5756752491314927 1103 32-33 0.6265892655235845 1103 34-35 0.5165180601421611 1103 36-37 0.6937584513389865 1103 38-39 0.9125330407398451 1103 40-41 0.8778973685294389 1103 42-43 0.5558437613866118 1103 44-45 0.8113398216334744 1103 46-47 0.8779485063379937 1103 48-49 1.0508176784958323 1103 50-51 0.7787431187922422 1103 52-53 0.6885276877963875 1103 54-55 0.5392421414079394 1103 56-57 0.6896029854810912 1103 58-59 0.9498513411600022 1103 60-61 0.8382616386822406 1103 62-63 0.37106339472534344 1103 64-65 0.44245568574479677 1103 66-67 0.27681660443403544 1103 68-69 -7.1077816133201175 1103 70-71 -7.8917206264080235 1103 72-73 -1.0845391589079476 1103 74-75 0.39863723042614296 1103 76-77 0.7076562081302811 1103 78-79 0.4930207355219203 1103 80-81 0.24896383785843668 1103 82-83 0.2465717745537397 1103 84-85 0.03356569382292207 1103 86-87 0.07896428384351495 1103 88-89 0.20046210214994886 1103 90-91 0.19711709184179327 1103 92-93 0.01670135810517337 1103 94-95 0.3040270209759832 1103 96-97 0.31556305866037704 1103 98-99 0.4840008588849898 1103 100-101 0.4498067191083379 1104 1 0.26364456468184017 1104 2 0.22537313189260644 1104 3 0.21252236514279588 1104 4 0.18138225200565472 1104 5 0.3469800339852682 1104 6 0.24541203045507132 1104 7 0.3588127179828149 1104 8 0.4948561288321969 1104 9 0.5632492353710532 1104 10-11 0.3129525768534549 1104 12-13 0.33403437360389177 1104 14-15 0.48852599710983213 1104 16-17 0.5183801631152392 1104 18-19 0.4714438870140185 1104 20-21 0.5474372342531879 1104 22-23 0.3844832661505322 1104 24-25 0.2781987506309278 1104 26-27 0.5573135074010267 1104 28-29 0.45710898903881514 1104 30-31 0.5096304558871978 1104 32-33 0.6477943460688138 1104 34-35 0.40147147700387364 1104 36-37 0.7118163016304209 1104 38-39 0.5611372676433248 1104 40-41 0.603303536779471 1104 42-43 0.5512428159554332 1104 44-45 0.6807647608229743 1104 46-47 0.6574444676028506 1104 48-49 0.5385299415643559 1104 50-51 0.6848494109580088 1104 52-53 0.5188996644818715 1104 54-55 0.6289709036274047 1104 56-57 0.5240641814974261 1104 58-59 0.543386496513623 1104 60-61 0.5848069138210725 1104 62-63 0.43068178014918246 1104 64-65 0.22454068276164207 1104 66-67 0.24935722125904292 1104 68-69 -6.6589834812351185 1104 70-71 -4.535352160668431 1104 72-73 0.030740339921738524 1104 74-75 0.8326670160978118 1104 76-77 1.0920158620841107 1104 78-79 0.6380675481337867 1104 80-81 0.274172085139174 1104 82-83 0.1491877734522653 1104 84-85 0.3093281198176925 1104 86-87 0.2746461646090381 1104 88-89 0.32958516883497424 1104 90-91 0.3907933734517819 1104 92-93 0.2231925922141258 1104 94-95 0.22735473220642533 1104 96-97 0.16756177360828417 1104 98-99 0.2989567999561693 1104 100-101 0.4573007527950601 1105 1 0.22647729678304884 1105 2 0.26314316079608346 1105 3 0.22259061435331873 1105 4 0.23910646037061412 1105 5 0.2668581134365837 1105 6 0.19479367487270594 1105 7 0.2726079565913153 1105 8 0.4548160951051017 1105 9 0.43940478211006706 1105 10-11 0.3755712083986893 1105 12-13 0.30485053570188114 1105 14-15 0.35420231253921486 1105 16-17 0.2726799474666848 1105 18-19 0.4186191662448664 1105 20-21 0.4957679646680404 1105 22-23 0.45310600121012357 1105 24-25 0.47817398277227596 1105 26-27 0.4923911401135541 1105 28-29 0.43177104918954257 1105 30-31 0.4080160157669326 1105 32-33 0.43884764512465324 1105 34-35 0.46546266071641185 1105 36-37 0.48939433758508954 1105 38-39 0.4649341077537983 1105 40-41 0.49613142615276473 1105 42-43 0.31732596639648136 1105 44-45 0.4496801617679722 1105 46-47 0.5268168508289008 1105 48-49 0.6882670315091346 1105 50-51 0.4041589572093116 1105 52-53 0.42477893493083485 1105 54-55 0.2418616949528456 1105 56-57 0.40170761530112387 1105 58-59 0.6644086369917197 1105 60-61 0.6816017503340959 1105 62-63 0.3976700085363625 1105 64-65 0.30087279604860484 1105 66-67 0.42472627381964756 1105 68-69 -3.3029399708345295 1105 70-71 -3.3700188686148635 1105 72-73 0.5641025117571665 1105 74-75 0.9364714814981063 1105 76-77 1.0059950440873564 1105 78-79 0.5963668638834712 1105 80-81 0.3971813876105337 1105 82-83 0.3539569049030895 1105 84-85 0.31820680470555374 1105 86-87 0.3455095691040988 1105 88-89 0.3652878650915845 1105 90-91 0.2842707502297692 1105 92-93 0.12452812637045696 1105 94-95 0.3730172609589282 1105 96-97 0.5742124596550369 1105 98-99 0.4650219478332822 1105 100-101 0.5242622640013685 1106 1 0.0732521275191651 1106 2 0.19769298586588135 1106 3 0.1370831091469391 1106 4 0.14394967873260356 1106 5 0.20373002423496445 1106 6 0.16950920062740238 1106 7 0.2807741855560053 1106 8 0.22873820151887259 1106 9 0.22559555224702876 1106 10-11 0.29419545478777565 1106 12-13 0.17058038364458383 1106 14-15 0.2380712564736882 1106 16-17 0.2140940455779372 1106 18-19 0.11212850505118865 1106 20-21 0.2716331045266571 1106 22-23 0.1440206557645496 1106 24-25 0.19550555850516105 1106 26-27 0.5180397679221826 1106 28-29 0.5308361496770928 1106 30-31 0.4235388003320111 1106 32-33 0.48455254588090924 1106 34-35 0.45538838626968214 1106 36-37 0.48489309772319444 1106 38-39 0.4991001898459402 1106 40-41 0.27790371641674483 1106 42-43 0.24874114875008502 1106 44-45 0.4169052837404337 1106 46-47 0.5920235518311898 1106 48-49 0.7237789005987949 1106 50-51 0.49991130318105803 1106 52-53 0.19729633231084875 1106 54-55 0.1595801952611069 1106 56-57 0.4463902235345607 1106 58-59 0.7066389158438575 1106 60-61 0.9732931280504928 1106 62-63 0.474208003445419 1106 64-65 0.38797821305033153 1106 66-67 0.45433022353771335 1106 68-69 -2.954025731906345 1106 70-71 -3.061722194143826 1106 72-73 0.8509882351111919 1106 74-75 1.3607098541041474 1106 76-77 1.3847908775977125 1106 78-79 1.0406223882809407 1106 80-81 0.7685711633203915 1106 82-83 0.5087375005789241 1106 84-85 0.5747777651962593 1106 86-87 0.629551030608976 1106 88-89 0.5396925747020695 1106 90-91 0.8473793269136785 1106 92-93 0.7044848008914748 1106 94-95 0.9704874293952095 1106 96-97 1.0426926786719939 1106 98-99 0.9120810269308777 1106 100-101 1.118561600248956 1107 1 -0.08324564533617362 1107 2 0.058587100517652146 1107 3 0.06677685868512384 1107 4 -0.12521737880209827 1107 5 0.2516112249815805 1107 6 0.10437244768063181 1107 7 -0.08460122445385565 1107 8 -0.06519509818910052 1107 9 0.013876558791125149 1107 10-11 -0.05045387634599052 1107 12-13 -0.022737158352313713 1107 14-15 -0.04212119115266688 1107 16-17 -0.12732003265885083 1107 18-19 0.09971064757559844 1107 20-21 0.13088853504905984 1107 22-23 0.26763200335974346 1107 24-25 0.12476440042205894 1107 26-27 0.3753895159729197 1107 28-29 0.2523514071841717 1107 30-31 0.11207471775819755 1107 32-33 0.07545094937238161 1107 34-35 -0.05485141930292059 1107 36-37 0.10413057174117313 1107 38-39 0.24544875284369994 1107 40-41 0.11198003366880727 1107 42-43 -0.12145037123397628 1107 44-45 0.20052860233857928 1107 46-47 0.32256198794674873 1107 48-49 0.32625757285754986 1107 50-51 0.2736934815261023 1107 52-53 0.10154156077589249 1107 54-55 0.07896627275577828 1107 56-57 0.649094521690845 1107 58-59 0.6442961833389553 1107 60-61 0.4141676778829506 1107 62-63 0.19642175351368607 1107 64-65 -0.024748717971633027 1107 66-67 0.2611677403637813 1107 68-69 -2.826425020472975 1107 70-71 -2.965909645753829 1107 72-73 0.8207610510958716 1107 74-75 1.1789891459155122 1107 76-77 0.6597868718696205 1107 78-79 0.7012120776372299 1107 80-81 0.5165385323983394 1107 82-83 0.3183513182727147 1107 84-85 0.3147059190997439 1107 86-87 0.2519644747560754 1107 88-89 0.1608910985862444 1107 90-91 0.142978266590422 1107 92-93 0.2858514790164648 1107 94-95 0.4455178889917697 1107 96-97 0.4330526380163633 1107 98-99 0.5075236729361521 1107 100-101 0.7345452484452331 1108 1 -0.06861639045179047 1108 2 -0.08840663086105138 1108 3 -0.06876477392489022 1108 4 0.06770422058620085 1108 5 -0.034059273607816465 1108 6 -0.10610260966637242 1108 7 0.1094425965707444 1108 8 0.12604399269602595 1108 9 0.13528001147654578 1108 10-11 0.10814438478723787 1108 12-13 -0.05783171500061002 1108 14-15 0.021379909646235262 1108 16-17 0.1584744705973975 1108 18-19 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100-101 0.918288946757233 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 37.0 4 80.0 5 198.0 6 530.0 7 1265.0 8 2894.0 9 4879.0 10 7616.0 11 11072.0 12 14493.0 13 18879.0 14 23494.0 15 28847.0 16 34030.0 17 37807.0 18 35772.0 19 32905.0 20 32660.0 21 33185.0 22 35284.0 23 39920.0 24 47031.0 25 58372.0 26 72583.0 27 90251.0 28 110241.0 29 131688.0 30 154587.0 31 178277.0 32 205452.0 33 237326.0 34 270473.0 35 300266.0 36 306962.0 37 245681.0 38 97614.0 39 5378.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.80330354861133 20.62148592961661 15.646557995252836 22.928652526519226 2 16.00438312291317 21.32235901711289 39.0126142040147 23.660643655959245 3 19.248369080987324 24.91720319956204 30.467709505736373 25.366718213714268 4 13.353786827733918 17.842312369189933 36.57822033259529 32.22568047048085 5 13.50740740995463 37.80189640148636 34.09980763636188 14.59088855219712 6 31.5944036417731 36.83536712431835 17.144400650061176 14.42582858384737 7 27.95032657802488 33.22406134178624 20.46980881241416 18.35580326777472 8 26.193607089875844 35.613063671195036 19.248949634013908 18.94437960491521 9 25.803824852116584 17.047909343563383 20.445187367135322 36.70307843718471 10-11 24.828857691723062 27.265395929725905 26.986647013873654 20.919099364677383 12-13 25.59674336682446 24.46432882146495 27.77195864972693 22.166969161983662 14-15 22.516965069871947 25.88534040523598 26.290562627534612 25.30713189735746 16-17 21.834985171199612 29.136026583003886 26.90521586225146 22.12377238354504 18-19 21.561184568654575 27.309270299574038 29.718049579285488 21.411495552485892 20-21 22.530788842221238 26.42623848276877 29.768135448210025 21.27483722679996 22-23 22.12385089694773 26.53680551328752 29.620492092754237 21.71885149701052 24-25 21.887242945956817 26.667886322358093 29.83285845282434 21.61201227886075 26-27 21.884230113334485 26.882887238771353 29.587120233869946 21.64576241402422 28-29 21.677234614634695 26.94644476440394 29.75484584459391 21.621474776367457 30-31 21.816696899098183 26.69413089639876 29.739634287875795 21.749537916627265 32-33 21.740161319829493 26.785257419845586 29.521387807203 21.953193453121926 34-35 22.133618299505066 26.860010368137665 29.398106396350006 21.60826493600727 36-37 22.028464925277333 26.64052414449147 29.434893383870037 21.89611754636116 38-39 21.823590791021914 26.93438328611747 29.571346725187837 21.670679197672786 40-41 22.113672040414002 26.7294532068265 29.504844494221867 21.65203025853764 42-43 21.94545585947717 26.8364811475297 29.232100821827295 21.985962171165838 44-45 22.115222139742393 27.014592734915226 29.030192090815454 21.839993034526927 46-47 22.113409122038078 27.12299684974661 28.615134293574435 22.14845973464088 48-49 22.26629455721097 27.15578871661812 28.527334387490516 22.0505823386804 50-51 22.172926836528415 27.40152407911832 28.319433411053147 22.106115673300124 52-53 22.37057352429879 27.367133689410867 28.197132559142535 22.065160227147807 54-55 22.10771708936928 27.31819567372895 28.330662413378594 22.243424823523178 56-57 22.176720876157454 27.677315942268265 28.08354315433194 22.062420027242332 58-59 22.23898197324543 27.340173627657567 28.176084123822818 22.24476027527418 60-61 22.28630386091921 27.27637750254715 28.43307983646658 22.004238800067064 62-63 22.21767341542621 27.30949617198135 28.322059418497425 22.150770994095016 64-65 22.087508936975368 27.220419256340485 28.325354392555635 22.36671741412852 66-67 21.896968448778686 27.489051507165595 28.30302493873115 22.31095510532457 68-69 22.047931867791238 26.958112649153932 28.889899050246292 22.104056432808537 70-71 21.53147551191557 26.872673061762892 29.33527944072828 22.260571985593256 72-73 22.037196899723458 27.133338153178947 28.46006105099437 22.369403896103222 74-75 22.07302597626011 27.39982797178737 27.982814381558573 22.544331670393944 76-77 21.409578522873918 27.505121193688186 28.493217422343132 22.59208286109476 78-79 21.9937313174637 27.54242847736566 27.8969957819577 22.566844423212938 80-81 21.823793393971698 27.623394487525577 27.878557059096615 22.67425505940611 82-83 22.025406517344948 27.63661135476896 27.733086794539467 22.60489533334663 84-85 21.937838975415644 27.5320595262494 27.687628867397425 22.84247263093753 86-87 21.98812302289666 27.431301945440456 27.848292577078443 22.73228245458444 88-89 21.89852972327601 27.423879582837007 27.72869137256089 22.948899321326095 90-91 21.81278493431874 27.57161766177529 27.862290644095566 22.753306759810407 92-93 21.904058730962234 27.4121497821111 27.849939337749802 22.833852149176863 94-95 21.63485346380033 27.312638663339605 27.89583769949712 23.156670173362944 96-97 21.700559520185212 27.107695165559836 27.93732617119976 23.25441914305519 98-99 21.602098474138703 27.06440437104157 27.75869679720969 23.574800357610037 100-101 21.53243476642958 27.04283193364302 27.98466949619747 23.44006380372993 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1773.0 1 1208.0 2 678.5 3 735.0 4 677.5 5 662.0 6 1089.0 7 2086.0 8 3131.0 9 3721.5 10 3657.0 11 3330.0 12 3267.0 13 3391.5 14 3595.5 15 3811.5 16 4123.5 17 4457.5 18 4827.5 19 5249.0 20 5826.5 21 6612.5 22 7696.0 23 9328.5 24 11262.5 25 13831.5 26 17755.5 27 22396.5 28 26376.0 29 30734.5 30 36033.5 31 41836.5 32 47924.0 33 53520.0 34 59719.5 35 65969.5 36 72442.0 37 78602.0 38 82310.0 39 83999.0 40 85934.0 41 90039.5 42 94632.5 43 98055.0 44 101521.5 45 104193.0 46 105514.5 47 107447.5 48 111775.5 49 113384.0 50 110517.0 51 107352.0 52 103800.5 53 100415.5 54 96140.0 55 88895.0 56 79958.5 57 71266.5 58 62803.0 59 54132.5 60 45293.5 61 36347.0 62 28166.5 63 21497.5 64 16037.0 65 11940.0 66 8902.0 67 6630.5 68 4792.5 69 3314.0 70 2327.5 71 1689.5 72 1256.0 73 1018.5 74 785.0 75 574.5 76 383.0 77 226.0 78 130.5 79 76.5 80 41.0 81 23.0 82 15.0 83 10.0 84 7.0 85 5.5 86 4.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021148308444811855 2 0.017812721584410635 3 3.7826242746817954E-4 4 0.0887541204813974 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.007565248549363591 12-13 0.0 14-15 0.0 16-17 3.6106868076508046E-4 18-19 1.719374670309907E-4 20-21 0.0 22-23 1.719374670309907E-4 24-25 0.003765430527978696 26-27 0.003198036886776427 28-29 9.284623219673497E-4 30-31 3.0948744065578324E-4 32-33 7.565248549363591E-4 34-35 0.002974518179636139 36-37 0.0833208965232181 38-39 0.09492667554780997 40-41 0.07597916668099479 42-43 0.0795726597419425 44-45 0.07816277251228837 46-47 0.07689043525625903 48-49 0.07149159879148592 50-51 0.07161195501840763 52-53 0.05655023290649284 54-55 0.04883024063680136 56-57 0.027303669764521324 58-59 0.020821627257452974 60-61 0.012774953800402609 62-63 0.0023211558049183747 64-65 0.005880261372459882 66-67 0.15261169573670735 68-69 0.7120446322154418 70-71 0.871946476554263 72-73 0.3539332758832944 74-75 0.05275041488510795 76-77 0.1937563315972234 78-79 0.013944128576213346 80-81 0.0028885494461206437 82-83 0.0013754997362479256 84-85 2.0632496043718884E-4 86-87 0.0018913121373408978 88-89 3.438749340619814E-4 90-91 0.004367211662587163 92-93 0.004470374142805759 94-95 0.07444892322441897 96-97 0.03710410538528779 98-99 0.013669028628963761 100-101 0.012620210080074717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2908034.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.26314035008909 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2681259010059 42.34821131456471 2 14.12675613736482 15.896313264960787 3 4.795160249820168 8.093723224104012 4 2.0917634675474233 4.707567262152388 5 1.0995192906697786 3.0931204084292077 6 0.6420106003615569 2.167291950863234 7 0.39561315966205135 1.5580907108485742 8 0.2867394968970694 1.2906291646267003 9 0.20706753095148736 1.0485242600282956 >10 0.9626747981735434 9.929283323731164 >50 0.07574256772055757 2.9294555261047934 >100 0.04542859252567994 4.542548247195302 >500 0.0027803514273844524 0.9843352808431122 >1k 4.3249911086444765E-4 0.40738187437549517 >5k 6.178558726634966E-5 0.24257280650159616 >10k+ 1.2357117453269933E-4 0.7609513806706706 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11532 0.39655657396027694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10358 0.35618565670140034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6978 0.2399559289884506 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2989 0.10278421779112625 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.4387493406198136E-5 0.0 4 0.0 0.0 0.0 3.4387493406198136E-5 0.0 5 0.0 0.0 0.0 3.4387493406198136E-5 0.0 6 0.0 0.0 0.0 3.4387493406198136E-5 0.0 7 6.877498681239627E-5 0.0 0.0 3.4387493406198136E-5 0.0 8 1.0316248021859442E-4 0.0 0.0 6.877498681239627E-5 0.0 9 1.0316248021859442E-4 0.0 0.0 1.0316248021859442E-4 3.4387493406198136E-5 10-11 1.0316248021859442E-4 0.0 0.0 1.0316248021859442E-4 3.4387493406198136E-5 12-13 1.0316248021859442E-4 0.0 0.0 1.0316248021859442E-4 3.4387493406198136E-5 14-15 1.0316248021859442E-4 0.0 0.0 1.3754997362479254E-4 3.4387493406198136E-5 16-17 1.0316248021859442E-4 0.0 0.0 1.5474372032789162E-4 3.4387493406198136E-5 18-19 1.0316248021859442E-4 0.0 0.0 2.57906200546486E-4 3.4387493406198136E-5 20-21 1.0316248021859442E-4 0.0 0.0 2.750999472495851E-4 3.4387493406198136E-5 22-23 1.3754997362479254E-4 0.0 0.0 2.750999472495851E-4 8.596873351549535E-5 24-25 2.4071245384338697E-4 0.0 0.0 3.438749340619814E-4 1.3754997362479254E-4 26-27 2.57906200546486E-4 0.0 0.0 5.330061477960711E-4 1.3754997362479254E-4 28-29 2.9229369395268416E-4 0.0 0.0 0.0015302434565758172 1.3754997362479254E-4 30-31 3.0948744065578324E-4 0.0 0.0 0.004539149129618154 1.3754997362479254E-4 32-33 3.0948744065578324E-4 0.0 0.0 0.009731660633954073 1.3754997362479254E-4 34-35 3.438749340619814E-4 0.0 0.0 0.01810501527836332 1.3754997362479254E-4 36-37 3.438749340619814E-4 0.0 0.0 0.03562544316882127 1.3754997362479254E-4 38-39 3.7826242746817954E-4 0.0 0.0 0.059163682405363904 1.3754997362479254E-4 40-41 3.954561741712786E-4 0.0 0.0 0.08543572736769928 1.3754997362479254E-4 42-43 4.4703741428057584E-4 0.0 0.0 0.11377102193440655 1.3754997362479254E-4 44-45 5.158124010929721E-4 0.0 0.0 0.14906978391586895 1.3754997362479254E-4 46-47 5.501998944991702E-4 0.0 0.0 0.1938423003307389 1.3754997362479254E-4 48-49 5.501998944991702E-4 0.0 0.0 0.2424662160071031 1.3754997362479254E-4 50-51 5.501998944991702E-4 0.0 0.0 0.2941334248499158 1.3754997362479254E-4 52-53 5.501998944991702E-4 0.0 0.0 0.3463508335872277 1.5474372032789162E-4 54-55 5.501998944991702E-4 0.0 0.0 0.4065805282881837 1.719374670309907E-4 56-57 5.845873879053683E-4 0.0 0.0 0.4783988082670285 1.719374670309907E-4 58-59 5.845873879053683E-4 0.0 0.0 0.5535870625996808 1.719374670309907E-4 60-61 5.845873879053683E-4 0.0 0.0 0.6310792789905483 1.719374670309907E-4 62-63 5.845873879053683E-4 0.0 0.0 0.7062331458297944 1.719374670309907E-4 64-65 5.845873879053683E-4 0.0 0.0 0.7907232171288231 1.719374670309907E-4 66-67 6.877498681239628E-4 0.0 0.0 0.8838273555261045 1.719374670309907E-4 68-69 7.565248549363591E-4 0.0 0.0 0.9856831109952635 1.719374670309907E-4 70-71 7.909123483425572E-4 0.0 0.0 1.115444317363552 1.719374670309907E-4 72-73 8.252998417487554E-4 0.0 0.0 1.2577741525718062 1.719374670309907E-4 74-75 8.424935884518544E-4 0.0 0.0 1.4088727985986407 1.719374670309907E-4 76-77 8.596873351549535E-4 0.0 0.0 1.5899573388756802 1.8913121373408977E-4 78-79 8.596873351549535E-4 0.0 0.0 1.7995147236930518 2.0632496043718884E-4 80-81 9.284623219673497E-4 0.0 0.0 2.0309769418101715 2.4071245384338697E-4 82-83 9.284623219673497E-4 0.0 0.0 2.274388813885945 2.750999472495851E-4 84-85 0.001014431055482845 0.0 0.0 2.5187979232704985 2.750999472495851E-4 86-87 0.0011175935357014394 0.0 0.0 2.782464028962522 2.750999472495851E-4 88-89 0.0011347872824045385 0.0 0.0 3.0743450729943325 2.750999472495851E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7300 0.0 49.55399 1 GTATCAA 12150 0.0 38.134678 1 ATCAACG 15880 0.0 28.715086 3 TATCAAC 15950 0.0 28.653 2 TCAACGC 16105 0.0 28.258879 4 CAACGCA 16330 0.0 27.845076 5 AACGCAG 16915 0.0 26.994186 6 GTGGTAT 3765 0.0 25.848505 1 TGGTATC 3750 0.0 25.437199 2 GTATACG 165 2.4440578E-6 23.017204 1 ACGCAGA 19765 0.0 22.885876 7 CGCAGAG 19835 0.0 22.781204 8 TACATGG 13570 0.0 22.137487 2 GTACATG 13915 0.0 22.107433 1 ACATGGG 13545 0.0 21.264093 3 GCAGAGT 20910 0.0 20.816353 9 GAGTACT 11580 0.0 20.571987 12-13 AGTACTT 12270 0.0 19.434446 12-13 GTACTTT 12375 0.0 19.288702 14-15 AGAGTAC 19530 0.0 19.056791 10-11 >>END_MODULE