FastQCFastQC Report
Fri 27 May 2016
SRR939461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023867
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104491.0205427072070883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84760.827841897433944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58990.5761490506091124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52670.5144222833629758No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31020.30296903797075203No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA25590.24993480598554302No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.19445885061243307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19240.18791503193285847No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19110.18664533577114997No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.14396401095064104No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11600.1132959651986049No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11200.10938920777796333No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG11170.10909620097141523No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT10300.10059900358151987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGG1051.6715821E-632.193485
GTATCAA164900.025.6915471
GAGTACT84550.025.65240112-13
GTACATG88250.024.7663861
TACATGG88800.024.3660772
GTACTTT91200.024.1043514-15
ATGGGAG21100.023.8018155
AGTACTT85150.023.75666412-13
GGTATCA121550.022.497221
CAACGCG2153.711466E-822.4605675
TATCAAC192550.022.1958452
ACTTTTT107700.022.03596516-17
ACATGGG93500.021.9237983
AACGCGG2255.7156285E-821.5627946
ATCAACG197550.021.4802993
TCAACGC199600.021.2219834
CAACGCA198750.020.968285
TACTTTT97650.020.76556814-15
CATGGGA71300.020.7430274
AACGCAG202550.020.4556456
Produced by FastQC (version 0.11.5)