FastQCFastQC Report
Fri 27 May 2016
SRR939461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023867
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140631.3735182401620523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135591.3242930966619686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89380.8729649456423538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86830.848059367085764No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38220.37329067154229995No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36920.3605937099252149No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36220.3537568844390922No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33440.32660492036563343No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30480.29769491545288596No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT24020.23460078310952498No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG17460.17052996141100357No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.16252110869868838No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15830.15460992492188927No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT14410.14074093607861177No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC14340.1400572535299995No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA14230.13898289523932308No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12840.12540691320259367No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12470.12179316258850027No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG11520.11251461371447659No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG10760.10509177461525765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG902.4582981E-531.580052
TAGCCCG856.5175677E-427.8593065
GGTATCA129800.025.2945081
ATAACGC1401.8201008E-523.6804083
GAGTACT101200.023.56341212-13
GCTTATA8150.022.671211
GTATCAA172000.022.5867181
GTACTTT106700.022.30441514-15
GTACATG97300.021.619111
TACATGG96550.021.4894772
AGTACTT101950.021.18345612-13
ACTTTTT120950.020.1464616-17
ACGGTGC1200.004844687419.7336738
ACATGGG99350.019.5926483
TACTTTT113350.019.07385414-15
TATCAAC202750.019.018122
ATCAACG206600.018.6600723
GTGTAGC4600.018.5388321
TCAACGC208100.018.5255684
TAACGCA2051.629792E-518.4822714
Produced by FastQC (version 0.11.5)