FastQCFastQC Report
Fri 27 May 2016
SRR939460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017206
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103921.0216219723438515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79770.784206935468332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58510.5752030562147686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52380.5149399433349784No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29270.28774899086320765No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA24180.2377099623871664No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.18885063595771162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19130.18806416792665398No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19120.18796585942277177No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.1455948942495424No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.10636980120054344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGA1652.5374902E-928.9018734
GAGTACT82150.024.13016712-13
GTACATG86400.023.9421981
GTATCAA163100.023.919251
CGTCACT1701.9443833E-623.6277071
TACATGG86600.023.253472
ACGCAGG4050.022.7345967
AGTACTT84250.022.61607712-13
GGTATCA124750.022.4984071
GTACTTT91300.022.06577314-15
ACATGGG90950.021.3751833
ATGAACG2951.2551027E-1021.0150894
ACTTTTT108850.020.60406316-17
ATGGGAT23800.020.1773225
TATCAAC189550.020.1646822
CATGGGG21800.020.1252464
CATGGGA68900.020.0719094
CAACGCG1706.768034E-519.9735095
ATCAACG197000.019.810063
TACTTTT97700.019.77024514-15
Produced by FastQC (version 0.11.5)