FastQCFastQC Report
Fri 27 May 2016
SRR939460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017206
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137361.350365609325938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128401.2622811898474842No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA89740.8822205138388881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85550.8410292507122451No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA35300.34702901870417596No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35120.34525946563429627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.342703444533359No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31540.31006502124446766No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30300.2978747667630745No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23100.22709264396788853No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG16890.16604306305704056No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.1570969892037601No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15200.14942892590094828No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT14660.14412026669130934No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA13470.1324215547293272No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC13400.13173339520215177No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.12514672544204417No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12410.12200085331781368No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG10690.10509179065007482No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG401.0934007E-535.55140718-19
CGACCGT559.6143514E-525.8555718-19
GGTATCA129400.024.4600331
CGACGAA500.001656938523.74295860-61
CGTCACT2252.8448994E-923.1993431
GAGTACG1252.2707577E-422.7775361
GAGTACT98650.022.75082212-13
GTATCAA168050.022.1382371
GTACATG94300.021.8898641
GTACTTT104350.021.5989314-15
TACATGG94300.021.5333752
AGTACTT100100.021.14275612-13
GTACACG1150.003744308620.6318241
GTATTAG2108.6874934E-720.3370861
ACATGGG95900.020.081773
CGGGAGT4300.019.8592434
TACACGA1200.004795820519.7682742
ACTTTTT117900.019.69907216-17
GTAGCCC3153.274181E-1019.5761533
GCACCGT1708.0770085E-519.519386
Produced by FastQC (version 0.11.5)