##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939460_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1017206 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.759409598449086 31.0 30.0 31.0 28.0 34.0 2 30.978706378059115 31.0 31.0 33.0 28.0 34.0 3 30.932195641787406 31.0 31.0 34.0 27.0 34.0 4 34.88576945082904 35.0 35.0 37.0 32.0 37.0 5 34.45710701667116 35.0 35.0 37.0 30.0 37.0 6 34.558288095036794 35.0 35.0 37.0 30.0 37.0 7 34.39433703694237 35.0 35.0 37.0 30.0 37.0 8 34.31544053023675 35.0 35.0 37.0 30.0 37.0 9 35.71700127604438 37.0 35.0 39.0 30.0 39.0 10-11 35.53273476562269 37.0 34.5 39.0 30.0 39.0 12-13 35.49586022890152 37.0 35.0 39.0 30.0 39.0 14-15 36.12909233724535 38.0 34.0 40.0 29.5 41.0 16-17 36.08309526290643 38.0 34.0 40.0 29.5 41.0 18-19 36.20081478088018 38.0 34.0 40.0 30.0 41.0 20-21 36.154812299573535 38.0 34.0 40.0 29.5 41.0 22-23 35.98366702516501 38.0 34.0 40.0 29.0 41.0 24-25 35.78921231294349 38.0 34.0 40.0 28.0 41.0 26-27 35.247567356071436 38.0 33.5 40.0 27.0 41.0 28-29 34.98553636136633 37.0 33.0 40.0 26.5 41.0 30-31 34.59828982526646 37.0 33.0 39.0 25.5 40.0 32-33 34.18157089124524 36.5 33.0 39.0 25.0 40.0 34-35 34.27993346480457 37.0 33.0 39.0 25.0 40.5 36-37 34.000848402388506 37.0 33.0 40.0 24.0 41.0 38-39 33.73237033599881 36.0 33.0 39.0 24.0 41.0 40-41 33.48152537440794 36.0 32.0 39.0 22.5 41.0 42-43 33.22300301020639 36.0 32.0 39.0 21.5 40.5 44-45 32.7209395147099 36.0 31.0 39.0 19.0 40.0 46-47 32.13977748853232 36.0 30.0 39.0 15.0 40.0 48-49 31.625446566378884 35.0 30.0 39.0 11.0 40.0 50-51 31.322242004077836 35.0 30.0 39.0 9.0 40.0 52-53 31.15532153762365 35.0 29.5 39.0 8.0 40.0 54-55 30.824886994374786 35.0 28.5 38.5 7.5 40.0 56-57 30.364624766271533 35.0 28.0 38.0 6.0 40.0 58-59 29.935464399541488 34.0 27.0 38.0 2.0 40.0 60-61 29.573872942157244 34.0 27.0 38.0 2.0 40.0 62-63 29.375696269978746 34.0 26.5 38.0 2.0 40.0 64-65 28.895791511257308 34.0 26.0 37.0 2.0 39.5 66-67 28.025052447586823 33.0 24.5 36.5 2.0 39.0 68-69 24.713166752850455 30.0 11.5 35.0 2.0 38.0 70-71 23.43884227973488 28.5 10.0 34.0 2.0 38.0 72-73 24.403815451344173 29.5 13.5 34.0 2.0 37.0 74-75 25.115408776589994 30.0 19.0 34.0 2.0 37.0 76-77 24.785111865246567 30.0 19.0 34.0 2.0 36.0 78-79 25.86600059378336 31.0 21.5 34.5 2.0 36.0 80-81 25.88636716653264 32.0 22.0 35.0 2.0 36.0 82-83 25.553973334801405 31.0 20.5 34.5 2.0 36.0 84-85 25.183789222635337 31.0 20.0 34.0 2.0 35.5 86-87 24.698127517926554 31.0 18.0 34.0 2.0 35.0 88-89 24.151515523895846 31.0 14.5 34.0 2.0 35.0 90-91 23.511626455211626 30.0 7.5 34.0 2.0 35.0 92-93 22.942154293230672 30.0 2.0 34.0 2.0 35.0 94-95 22.10734059767638 29.0 2.0 34.0 2.0 35.0 96-97 21.40224202373954 29.0 2.0 34.0 2.0 35.0 98-99 20.538359486672316 29.0 2.0 33.5 2.0 35.0 100-101 19.236141450207725 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2788344139673953 1101 2 0.2908596205124603 1101 3 0.3463380820083444 1101 4 0.3994595046084157 1101 5 0.4177366037051726 1101 6 0.47239359038599815 1101 7 0.5356958078945553 1101 8 0.6717139024938632 1101 9 0.8266371188326431 1101 10-11 0.7352341829024311 1101 12-13 0.7176283413037154 1101 14-15 0.8303242354362652 1101 16-17 0.7738646465610799 1101 18-19 0.6039863120034781 1101 20-21 0.8741372282357105 1101 22-23 0.9080455729834682 1101 24-25 0.8403361333790684 1101 26-27 0.9376708149191373 1101 28-29 0.8794152872967871 1101 30-31 0.5771291187956322 1101 32-33 1.0433025697366602 1101 34-35 1.156382566134262 1101 36-37 1.2351462003437206 1101 38-39 1.1212091408756137 1101 40-41 1.2541316832267029 1101 42-43 0.975453571703774 1101 44-45 1.220027151133479 1101 46-47 0.9380899991277403 1101 48-49 1.2167269146228996 1101 50-51 1.2040442870396042 1101 52-53 0.8395949711626223 1101 54-55 0.896064407124161 1101 56-57 1.0348483579102457 1101 58-59 1.2445186708469826 1101 60-61 1.1236651294606297 1101 62-63 0.7574869747151958 1101 64-65 0.5491838627927876 1101 66-67 0.32541576848695897 1101 68-69 -6.422571048500373 1101 70-71 -7.051196033276199 1101 72-73 -3.9703233423669957 1101 74-75 -2.5038395104997733 1101 76-77 0.24902127015219122 1101 78-79 0.5237288050183437 1101 80-81 0.7303052199954045 1101 82-83 0.482247548371582 1101 84-85 0.13428957851868262 1101 86-87 -0.010997998379146168 1101 88-89 0.10104709624235753 1101 90-91 0.02626093892057213 1101 92-93 0.13291561606756375 1101 94-95 0.056530643296735406 1101 96-97 0.02189094555440363 1101 98-99 -0.09794175956816531 1101 100-101 -0.3401319558571778 1102 1 0.2544940833644276 1102 2 0.3174414043419773 1102 3 0.31125258581934645 1102 4 0.38266453991092675 1102 5 0.28746598275282054 1102 6 0.39873142458371547 1102 7 0.3357064308956339 1102 8 0.47499944496879465 1102 9 0.5235659333436615 1102 10-11 0.5605047848851399 1102 12-13 0.5731710818103579 1102 14-15 0.8240999155928819 1102 16-17 0.5665203588902088 1102 18-19 0.5376874035168342 1102 20-21 0.4843535201254312 1102 22-23 0.5208576710641211 1102 24-25 0.3775720284996993 1102 26-27 0.6132200809835808 1102 28-29 0.22946707442702774 1102 30-31 0.27615445844712383 1102 32-33 0.701875900692464 1102 34-35 0.3533257975753159 1102 36-37 0.6801972097185214 1102 38-39 0.5851300146933269 1102 40-41 0.49292233043303924 1102 42-43 0.5053091368284655 1102 44-45 0.3756112454657341 1102 46-47 0.6654207045329485 1102 48-49 0.8169352013225186 1102 50-51 0.5920254995034178 1102 52-53 0.12779054061238782 1102 54-55 0.29461816140656083 1102 56-57 0.387891278628512 1102 58-59 0.3707419283902631 1102 60-61 0.5538693566562713 1102 62-63 0.16829549891476958 1102 64-65 0.2687525689491963 1102 66-67 0.03616700435931719 1102 68-69 -6.737443629396427 1102 70-71 -7.2385842546443655 1102 72-73 -1.669196924860291 1102 74-75 -0.21130823904036333 1102 76-77 0.15193956030429234 1102 78-79 -0.018598542759740155 1102 80-81 -0.18060536089306112 1102 82-83 -0.28633264959562865 1102 84-85 -0.5587622199933406 1102 86-87 -0.6908215048041626 1102 88-89 -0.5291886205636089 1102 90-91 -0.6848048136621436 1102 92-93 -0.7027177234625199 1102 94-95 -0.5382360111028959 1102 96-97 -0.9203529357866742 1102 98-99 -0.9220593865414806 1102 100-101 -0.6660482845480082 1103 1 0.313629292617545 1103 2 0.2047576688147501 1103 3 0.393831620908621 1103 4 0.32048258633019344 1103 5 0.5592918721007436 1103 6 0.4614327431650196 1103 7 0.5146044018998452 1103 8 0.6317523720301494 1103 9 0.6083997365434826 1103 10-11 0.5568725544082369 1103 12-13 0.446386918970596 1103 14-15 0.6528738447996645 1103 16-17 0.7359888630599087 1103 18-19 0.7134518230987439 1103 20-21 0.6240135761547876 1103 22-23 0.5508670683938561 1103 24-25 0.434102286128379 1103 26-27 0.5671029807985875 1103 28-29 0.5854382204875321 1103 30-31 0.6301227616676286 1103 32-33 0.5462865580918219 1103 34-35 0.8618291989358582 1103 36-37 1.1736900021439212 1103 38-39 1.390081121982469 1103 40-41 1.1110320634661335 1103 42-43 0.9408413862182314 1103 44-45 1.3634364580877048 1103 46-47 1.4418296390885246 1103 48-49 1.5641662396979008 1103 50-51 1.066722524273363 1103 52-53 1.1527477650312647 1103 54-55 1.055616066215748 1103 56-57 1.3036159959698388 1103 58-59 1.749163236096667 1103 60-61 1.197094413838137 1103 62-63 0.9843235608780461 1103 64-65 1.0099167148855877 1103 66-67 0.9552081221096351 1103 68-69 -6.254199245630758 1103 70-71 -7.114149424239567 1103 72-73 -0.5273681690312877 1103 74-75 1.0258243637835633 1103 76-77 1.3852921758944028 1103 78-79 1.269578848092344 1103 80-81 0.9332955209192626 1103 82-83 0.7419258301646465 1103 84-85 0.49677806736400143 1103 86-87 0.7819749986799422 1103 88-89 0.6349273950867875 1103 90-91 0.5414909392176348 1103 92-93 0.4823723638321695 1103 94-95 0.8941342436888569 1103 96-97 0.8301812048895805 1103 98-99 0.9265989082273407 1103 100-101 0.9082353168160857 1104 1 0.20570701794671464 1104 2 0.3104528273596827 1104 3 0.2903189776741968 1104 4 0.3616323222348683 1104 5 0.24247911948464917 1104 6 0.26142587025248076 1104 7 0.2824169439415982 1104 8 0.6465591115789309 1104 9 0.40831828521832847 1104 10-11 0.43829470624403655 1104 12-13 0.46671611685783176 1104 14-15 0.596452716589134 1104 16-17 0.399006671214984 1104 18-19 0.3654393377950811 1104 20-21 0.33808981284034445 1104 22-23 0.37282646264166885 1104 24-25 0.3537286921508027 1104 26-27 0.7224271482987845 1104 28-29 0.5438424764289422 1104 30-31 0.5545448520859111 1104 32-33 0.6323427346467128 1104 34-35 0.478433278228799 1104 36-37 0.6231609341108566 1104 38-39 0.7350687946095533 1104 40-41 0.7382624698161209 1104 42-43 0.6311640329037331 1104 44-45 0.7782660829729124 1104 46-47 0.6801131511175882 1104 48-49 0.8482997555290019 1104 50-51 0.8086867003135119 1104 52-53 0.6655960900878526 1104 54-55 0.5845477377665667 1104 56-57 0.5172707183919094 1104 58-59 0.46665874676869024 1104 60-61 0.8588286690191431 1104 62-63 0.5795177864616612 1104 64-65 0.4998895741926823 1104 66-67 0.34323521600824236 1104 68-69 -5.931388780085982 1104 70-71 -3.594662523718398 1104 72-73 0.5509918078055378 1104 74-75 1.1437189420792002 1104 76-77 1.240660871093894 1104 78-79 0.8933278317786844 1104 80-81 0.6900006320264751 1104 82-83 0.34604168114820766 1104 84-85 0.6131306273045745 1104 86-87 0.4383308342213823 1104 88-89 0.23414775821054334 1104 90-91 0.3716521309514249 1104 92-93 0.5130171453905383 1104 94-95 0.9449835601996384 1104 96-97 0.7617291895176521 1104 98-99 0.45736506249644293 1104 100-101 0.9716573107211239 1105 1 0.14196303575335634 1105 2 0.11187593780909566 1105 3 0.18203545665978993 1105 4 0.290362104402476 1105 5 0.3549131326531594 1105 6 0.30682451433131064 1105 7 0.2829430626653533 1105 8 0.37709086887466725 1105 9 0.37222032501983904 1105 10-11 0.27511798511064 1105 12-13 0.14217005150071316 1105 14-15 0.27212124255394343 1105 16-17 0.2323979688709059 1105 18-19 0.39597272302498254 1105 20-21 0.32036530961925536 1105 22-23 0.3162346612141178 1105 24-25 0.5202837466644041 1105 26-27 0.715420741637864 1105 28-29 0.44954771970891017 1105 30-31 0.31405235760238526 1105 32-33 0.28007887019738575 1105 34-35 0.32879911943417994 1105 36-37 0.37137871618374874 1105 38-39 0.33471168505106874 1105 40-41 0.5252757729981425 1105 42-43 0.21565492609200732 1105 44-45 0.333597715361698 1105 46-47 0.2426195333594876 1105 48-49 0.6002288575161181 1105 50-51 0.35423481834761716 1105 52-53 0.14418801583037677 1105 54-55 0.3080697585360497 1105 56-57 0.44857337651241735 1105 58-59 0.46939108627288206 1105 60-61 0.49325866267020047 1105 62-63 0.3331884080217229 1105 64-65 0.14726201445119713 1105 66-67 0.12056342085563898 1105 68-69 -3.492151052859672 1105 70-71 -3.48918015942521 1105 72-73 0.5480559765305522 1105 74-75 0.9055780724959668 1105 76-77 0.7622028799350566 1105 78-79 0.4420852000307107 1105 80-81 0.29581191301827303 1105 82-83 0.28821301973927405 1105 84-85 0.33660676992936445 1105 86-87 0.11692705179034135 1105 88-89 0.11433994323857988 1105 90-91 0.29828853777512876 1105 92-93 0.11185457840474555 1105 94-95 0.36923874332003237 1105 96-97 0.42315514145215616 1105 98-99 0.3660557150464534 1105 100-101 0.5212127366996988 1106 1 0.11273606576158812 1106 2 0.07679848514128196 1106 3 0.2835964833753444 1106 4 0.2411037043426134 1106 5 0.25067755680940706 1106 6 0.2977666680393156 1106 7 0.3301974217164698 1106 8 0.4260484382827343 1106 9 0.40458281847190847 1106 10-11 0.5326881415126437 1106 12-13 0.12489623574077768 1106 14-15 0.5144081541755909 1106 16-17 0.5987535776413964 1106 18-19 0.48303700499759117 1106 20-21 0.40483212096400933 1106 22-23 0.45597798232464726 1106 24-25 0.4819727386572197 1106 26-27 0.7186542969772347 1106 28-29 0.44956717641773025 1106 30-31 0.6846495288962586 1106 32-33 0.8364314856280544 1106 34-35 0.6450649280100933 1106 36-37 0.518880774104872 1106 38-39 0.7478561885308252 1106 40-41 0.8840215636140201 1106 42-43 0.5231809307316908 1106 44-45 0.8263875080729122 1106 46-47 0.8763926382012173 1106 48-49 0.8949441160658473 1106 50-51 0.6078605012032661 1106 52-53 0.4391696816239872 1106 54-55 0.4045682992828894 1106 56-57 0.6074950258426561 1106 58-59 0.7168976342037325 1106 60-61 0.9042195247520688 1106 62-63 0.36990770758020375 1106 64-65 0.5296357329275878 1106 66-67 0.7100350963775988 1106 68-69 -2.904758251841937 1106 70-71 -2.905169832402837 1106 72-73 0.7350364449882356 1106 74-75 1.225148528231955 1106 76-77 1.0762379020109378 1106 78-79 0.8250920098787979 1106 80-81 0.8651182065152447 1106 82-83 0.7572940644148716 1106 84-85 0.2899398538177138 1106 86-87 0.44735210604462594 1106 88-89 0.3895244078049167 1106 90-91 0.5218865322374526 1106 92-93 0.5043547654884861 1106 94-95 0.7508955569097999 1106 96-97 0.7112947807547094 1106 98-99 0.8370876689490174 1106 100-101 0.7960784105753262 1107 1 0.08418534112390574 1107 2 -0.005701514858717616 1107 3 0.14113271525940263 1107 4 -0.07328035362840524 1107 5 0.25085871622970046 1107 6 0.1313898564451108 1107 7 -0.010998230457445857 1107 8 0.03677307596389312 1107 9 0.3025176010806021 1107 10-11 -0.08169591645836505 1107 12-13 0.13589261255237517 1107 14-15 0.023520473016169774 1107 16-17 -0.10131888612672668 1107 18-19 0.19271091804107243 1107 20-21 0.21611835284807768 1107 22-23 0.18095986638262218 1107 24-25 0.3149437531499686 1107 26-27 0.4345238621946308 1107 28-29 0.32766500250468766 1107 30-31 0.3523850361426426 1107 32-33 0.3669387320048685 1107 34-35 -0.025297390830480992 1107 36-37 0.27426120888748784 1107 38-39 0.431008362443869 1107 40-41 0.4285324331792353 1107 42-43 0.17874252493458442 1107 44-45 0.2845396819859616 1107 46-47 0.4281317686359998 1107 48-49 0.5754332465006264 1107 50-51 0.6444547041018147 1107 52-53 0.2881551888703626 1107 54-55 0.34697771957274526 1107 56-57 0.7511906780165667 1107 58-59 1.0040896631892409 1107 60-61 0.6262666262013461 1107 62-63 0.5447808959860048 1107 64-65 0.30066834162323985 1107 66-67 0.4094191543486154 1107 68-69 -2.520827092421648 1107 70-71 -2.5102785280550073 1107 72-73 1.1526451406404092 1107 74-75 1.5826122963478966 1107 76-77 1.3845712353442678 1107 78-79 1.354566647559956 1107 80-81 1.0611145833268374 1107 82-83 0.7567143542699419 1107 84-85 0.7083094190351069 1107 86-87 0.5789100770591205 1107 88-89 0.7669519440368013 1107 90-91 0.7075568220925241 1107 92-93 0.8713475191116729 1107 94-95 1.1216926583590734 1107 96-97 0.9288853604648537 1107 98-99 1.0013086834417741 1107 100-101 1.15908565695214 1108 1 0.03636192810716565 1108 2 0.02635568071323746 1108 3 -0.0604288655737939 1108 4 0.09275459476455694 1108 5 -0.04053052918449396 1108 6 -0.33773950876158665 1108 7 -5.197289332983246E-4 1108 8 0.09707041806775152 1108 9 0.04418493623221309 1108 10-11 0.11422030900823188 1108 12-13 -0.0892614732276158 1108 14-15 0.12599085913307562 1108 16-17 0.4531158430031752 1108 18-19 0.30215861577891445 1108 20-21 0.16458986201968884 1108 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1.4236419944016916 2312 90-91 1.6591544940012497 2312 92-93 1.741934167276952 2312 94-95 1.8676711761923563 2312 96-97 2.091392426967065 2312 98-99 2.3951152154594517 2312 100-101 1.8770230091980018 2313 1 -0.2541789987562133 2313 2 -0.28646730746896765 2313 3 -0.14883031871824315 2313 4 -0.07148063519203163 2313 5 0.17222494759553797 2313 6 0.16493565803775567 2313 7 -2.3217816423867603 2313 8 -3.0906992427039484 2313 9 -0.9164121548400033 2313 10-11 -0.11304901970412828 2313 12-13 -0.1842958666585517 2313 14-15 -0.755046683363922 2313 16-17 -0.46294461378732876 2313 18-19 0.15918562913605427 2313 20-21 -2.761508242823105 2313 22-23 -0.5784657135866311 2313 24-25 0.039043785970264366 2313 26-27 -1.3345788480712173 2313 28-29 -1.398165113575537 2313 30-31 -0.7583732207539455 2313 32-33 -0.7128513044429354 2313 34-35 -1.4237898162506681 2313 36-37 -1.8601330622427454 2313 38-39 -1.2370974312028054 2313 40-41 -0.6156535154138041 2313 42-43 0.010553061114642048 2313 44-45 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66-67 -1.672162401571537 2314 68-69 -1.6906042321536674 2314 70-71 0.9645471487292419 2314 72-73 0.7088900134870038 2314 74-75 0.40998958647641714 2314 76-77 0.4181626353989607 2314 78-79 -1.8104935110399865 2314 80-81 -1.2686041068563725 2314 82-83 -0.07379904469095422 2314 84-85 0.2569661048431442 2314 86-87 0.08110620776734834 2314 88-89 -0.11638822550107264 2314 90-91 0.16201060934983857 2314 92-93 -0.22110360202587032 2314 94-95 0.5447768120369503 2314 96-97 0.4159051170959742 2314 98-99 0.2752183088177631 2314 100-101 0.4130164745622018 2315 1 -0.3534153029984779 2315 2 -0.31476662835931535 2315 3 -0.5962965091964669 2315 4 -0.38270908460341957 2315 5 -0.4285443017953412 2315 6 -0.256434152697274 2315 7 -1.3050381504615984 2315 8 -3.5527287680961557 2315 9 -1.2718973643757394 2315 10-11 -1.6349431503682936 2315 12-13 -0.8790952452212508 2315 14-15 -0.5841378953802803 2315 16-17 -0.21673496840816853 2315 18-19 -0.4447007748579992 2315 20-21 -1.0493110404301973 2315 22-23 -0.48697459156761624 2315 24-25 -5.075717770926705 2315 26-27 -0.9527352412285737 2315 28-29 -1.0451484227719234 2315 30-31 -1.38385756164449 2315 32-33 -0.5203203714183289 2315 34-35 -2.3175224700471446 2315 36-37 -3.8524968166696283 2315 38-39 -1.1180702568486325 2315 40-41 -0.18935592326977968 2315 42-43 -1.365652896082505 2315 44-45 -4.812192305962718 2315 46-47 -3.1589935755019667 2315 48-49 -2.261297637609289 2315 50-51 -1.0375812940281541 2315 52-53 -0.26706272188751967 2315 54-55 -1.282448998519822 2315 56-57 -1.3738612673369168 2315 58-59 -0.863038992465011 2315 60-61 -1.674391456495659 2315 62-63 -0.6212283373005825 2315 64-65 -0.043779674064257534 2315 66-67 -0.07522125394186219 2315 68-69 2.674969554505786 2315 70-71 3.42264378766885 2315 72-73 -2.599101521870626 2315 74-75 -2.1879645568025907 2315 76-77 -0.371798563627209 2315 78-79 -0.6342991905783215 2315 80-81 -0.7190997561879975 2315 82-83 -1.170912781982718 2315 84-85 -0.6426106630385533 2315 86-87 -0.3497332401711546 2315 88-89 -0.17461927304283265 2315 90-91 -0.1516566603239724 2315 92-93 -1.5087203994380118 2315 94-95 -0.43499920180403606 2315 96-97 0.06290924234112083 2315 98-99 -2.3593332961956506 2315 100-101 -3.3889280825938606 2316 1 -0.4615135836072781 2316 2 -0.45792802257260234 2316 3 -0.9192927171856624 2316 4 -0.2739874597527603 2316 5 -0.10991150349914847 2316 6 -0.4413076657615278 2316 7 -0.8478670944125639 2316 8 -1.3631801732179696 2316 9 -5.582738359648712 2316 10-11 -1.3577887168323741 2316 12-13 -0.7256226983405654 2316 14-15 -0.6453814671427907 2316 16-17 -0.2786798024427597 2316 18-19 -0.248996655591462 2316 20-21 -3.734340389554916 2316 22-23 -1.1599266460063475 2316 24-25 -0.516344231889768 2316 26-27 -0.5232250859119674 2316 28-29 0.048431131536951 2316 30-31 0.24595387672234637 2316 32-33 -1.2951112913705387 2316 34-35 -0.622130022901537 2316 36-37 -0.44615428900872445 2316 38-39 -5.535944652983897 2316 40-41 -5.198965813665083 2316 42-43 -3.7386984521575144 2316 44-45 -2.5865157177335334 2316 46-47 -2.6241122706346864 2316 48-49 -3.0118300774825464 2316 50-51 -1.4239768059496107 2316 52-53 -1.2134810897645139 2316 54-55 -0.3289660625684441 2316 56-57 -3.4705835155318177 2316 58-59 -1.0596521554175844 2316 60-61 -0.47785621157331803 2316 62-63 -0.3323082811995981 2316 64-65 -0.1115423876895214 2316 66-67 -0.04327486154666005 2316 68-69 2.209435296545159 2316 70-71 3.3762046416504603 2316 72-73 2.3775750144131997 2316 74-75 1.0331148676428938 2316 76-77 -1.4924156744266526 2316 78-79 -0.028512478199743896 2316 80-81 0.11457122194301306 2316 82-83 0.3108284262662053 2316 84-85 0.5151993208583896 2316 86-87 0.46212068949483864 2316 88-89 0.4269858383799523 2316 90-91 0.488310094649794 2316 92-93 0.4121668271995631 2316 94-95 0.7283429622393882 2316 96-97 1.1013228312455965 2316 98-99 1.2986584964384988 2316 100-101 1.0221934175879461 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 17.0 4 67.0 5 186.0 6 486.0 7 960.0 8 1821.0 9 2966.0 10 4600.0 11 6344.0 12 7811.0 13 9881.0 14 11989.0 15 15610.0 16 18273.0 17 19661.0 18 16813.0 19 13931.0 20 13155.0 21 13400.0 22 14501.0 23 15982.0 24 18385.0 25 22303.0 26 27842.0 27 33613.0 28 40474.0 29 47504.0 30 54799.0 31 62627.0 32 71487.0 33 81164.0 34 92015.0 35 97374.0 36 87693.0 37 65724.0 38 24571.0 39 1172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69617844728833 21.455169396113057 16.426826090590417 19.42182606600819 2 15.843515589741674 23.7000846568546 38.1331442893003 22.32325546410343 3 19.61582927318414 28.376313895737727 31.583045288850613 20.424811542227516 4 12.706237018714697 19.492466795950676 36.87949333262493 30.9218028527097 5 11.965914475533964 40.38650971386327 32.8662040923864 14.781371718216368 6 27.97348816267305 38.34011989705133 17.49036085119435 16.196031089081266 7 25.04910509768916 37.342583508158626 20.243195576903794 17.365115817248427 8 26.29526369289996 34.25314046515652 19.427431611689276 20.024164230254247 9 26.75485594854926 17.49930692504763 20.83294829169313 34.91288883470998 10-11 24.167671535096495 28.829654769319408 25.608635658221186 21.394038037362918 12-13 26.802388110176306 26.673358198830915 27.170750074222923 19.353503616769856 14-15 23.540413642860937 26.983521528579264 28.17895293578685 21.29711189277295 16-17 19.632795368848853 30.823334875149737 28.74650326362062 20.79736649238079 18-19 19.152334091948035 27.973122428615664 33.71110051562861 19.16344296380769 20-21 20.98517900995472 26.454523469189134 33.588525824660884 18.971771696195265 22-23 20.11476568590537 26.92146995240871 33.34693271647842 19.616831645207494 24-25 19.80191060071748 27.04585382734595 34.03364442613652 19.11859114580005 26-27 20.131610704835442 26.892745681262603 34.04870294096394 18.92694067293801 28-29 19.361821877373263 27.243220924259663 34.40681090354087 18.9881462948262 30-31 19.335256251450055 27.772501877696115 33.902786461712694 18.989455409141136 32-33 19.158837159690485 27.86745759679276 33.514287764734334 19.45941747878242 34-35 19.546674951065356 27.303074304960905 33.662513653036406 19.487737090937333 36-37 20.056150037780995 27.231636861658586 33.63817218688999 19.074040913670423 38-39 20.157830519148266 27.215629550911167 33.58454362970835 19.04199630023222 40-41 19.684894896918653 27.502313187653755 33.75901233158986 19.05377958383774 42-43 19.343578441982643 28.066842447019937 33.065317486865666 19.524261624131757 44-45 19.540275118699295 28.21550636834266 32.40746570854576 19.83675280441229 46-47 19.68214756015196 28.356731023226505 31.46520746541997 20.495913951201565 48-49 20.15533617640838 28.329422875440848 31.172448733651088 20.34279221449968 50-51 20.434950703684976 28.889499914412703 30.861303327279916 19.8142460546224 52-53 20.161834086863976 29.102692792122433 30.672787371570692 20.062685749442906 54-55 19.612131178659542 28.916615973377542 30.823699969166025 20.647552878796898 56-57 20.271058939217014 29.39413680781759 29.81422162128941 20.52058263167599 58-59 20.330256537331103 28.702750433396563 30.246015849088515 20.720977180183823 60-61 20.2244594866309 28.5864086432978 30.707953420895706 20.481178449175594 62-63 21.027383894088146 28.489346338499715 30.452066019423203 20.031203747988936 64-65 20.708614698377165 28.617067767007377 30.59364030204313 20.080677232572327 66-67 20.129181804261847 28.8973758438199 30.662092441943763 20.311349909974492 68-69 20.851550443648957 28.552460325164276 30.735789663444873 19.860199567741894 70-71 20.395959443624793 28.6582939851034 30.666070696838215 20.27967587443359 72-73 20.378182356423917 28.71353858298796 29.996447602131436 20.911831458456682 74-75 20.137306861506865 29.22917724083932 29.90558001550223 20.727935882151584 76-77 19.645680577597695 29.017408119980182 30.633158670910582 20.703752631511545 78-79 19.74834326391757 28.892985664562566 30.56589581735591 20.79277525416396 80-81 19.459517649782658 29.750025929900296 30.094414330804575 20.696042089512474 82-83 19.534518828451883 29.588648661402463 29.532857378802657 21.343975131343 84-85 19.58382979225406 28.997084164041826 29.587771971010728 21.831314072693385 86-87 19.777306651159794 29.09897771641007 29.656546729277462 21.467168903152675 88-89 20.03231414818008 28.549554832547923 29.832781410566145 21.585349608705847 90-91 19.475560431868278 29.014650566969667 30.015965991651285 21.493823009510777 92-93 19.308201946397865 29.286219192438562 29.84596342171987 21.559615439443707 94-95 19.122111474113034 29.192061359514078 29.754159531391355 21.931667634981537 96-97 19.03696939566808 28.863036554368332 29.51665543044088 22.583338619522706 98-99 19.389170747055235 28.701354885650797 29.35574105755806 22.553733309735904 100-101 19.523366608624176 28.608966335683544 29.75362585306237 22.114041202629906 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7485.0 1 5013.0 2 2553.5 3 2585.5 4 2303.0 5 2238.0 6 3586.0 7 6371.5 8 8742.5 9 9627.5 10 8372.5 11 6155.5 12 5045.5 13 4434.5 14 4018.0 15 3730.0 16 3442.5 17 3370.0 18 3458.5 19 3493.0 20 3616.5 21 4011.0 22 4523.0 23 5011.0 24 5612.0 25 6488.0 26 7735.5 27 9059.0 28 10402.0 29 11921.5 30 13611.0 31 15692.5 32 17989.5 33 19840.0 34 21605.5 35 23294.0 36 24947.0 37 26723.0 38 27569.0 39 27610.0 40 27888.5 41 28773.5 42 29619.0 43 30297.5 44 30868.0 45 31582.0 46 33101.5 47 36570.5 48 45602.0 49 45701.0 50 36588.5 51 31872.0 52 28347.5 53 26189.0 54 24502.0 55 22294.5 56 20045.5 57 17902.5 58 15997.0 59 14508.5 60 13189.0 61 11539.0 62 9355.5 63 6905.5 64 4821.0 65 3465.5 66 2568.5 67 1915.5 68 1390.0 69 937.0 70 681.5 71 592.0 72 498.5 73 436.0 74 358.0 75 262.5 76 183.5 77 108.5 78 72.5 79 55.0 80 33.0 81 16.5 82 12.0 83 7.5 84 3.0 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020743094319144794 2 0.01563105211727025 3 1.9661700776440563E-4 4 0.08248083475716816 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.00732398353922411 12-13 0.0 14-15 0.0 16-17 7.373137791165212E-4 18-19 9.830850388220282E-5 20-21 0.0 22-23 2.9492551164660846E-4 24-25 0.00383403165140591 26-27 0.0038831859033470113 28-29 0.001327164802409738 30-31 1.9661700776440563E-4 32-33 6.881595271754197E-4 34-35 0.003244180628112693 36-37 0.08080959019117072 38-39 0.09103367459491982 40-41 0.07387884066747542 42-43 0.07727048405141142 44-45 0.07589416499706057 46-47 0.07466530869853304 48-49 0.06891426122142418 50-51 0.06881595271754198 52-53 0.05382390587550604 54-55 0.046254151076576426 56-57 0.02639583329237146 58-59 0.023495732427846472 60-61 0.013812344795449495 62-63 0.002015324329585158 64-65 0.006537515508166488 66-67 0.16324127069639777 68-69 0.7495040335979143 70-71 0.9167267987015414 72-73 0.3741621657756639 74-75 0.05682231524391323 76-77 0.20693940067203695 78-79 0.014353041566801613 80-81 0.003981494407229214 82-83 0.0021627870854084618 84-85 1.9661700776440563E-4 86-87 0.0022119413373495635 88-89 4.423882674699127E-4 90-91 0.0043255741708169235 92-93 0.004423882674699127 94-95 0.08213675499358045 96-97 0.03986409832423324 98-99 0.014353041566801613 100-101 0.01327164802409738 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1017206.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.945838564871664 #Duplication Level Percentage of deduplicated Percentage of total 1 77.02935980251637 42.32442768464478 2 13.045384846240255 14.335792195562803 3 4.480976709230315 7.386330686349564 4 1.9729991257017303 4.336323657977608 5 1.0485604278634713 2.880701599744952 6 0.5993454412100409 1.9758922714391212 7 0.3878293190107148 1.4916725007160796 8 0.2551183571717487 1.1214153654475338 9 0.19005545240496274 0.9398480585595059 >10 0.8880874729220398 8.50861285018781 >50 0.05640542991165481 2.14478149302083 >100 0.03862423818675275 3.6924006205775037 >500 0.0038080232558379418 1.3223653963405866 >1k 0.0027200166113128155 3.1450017883883823 >5k 3.6266888150837537E-4 1.7465146950312043 >10k+ 3.6266888150837537E-4 2.6479191360117107 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13736 1.350365609325938 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12840 1.2622811898474842 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8974 0.8822205138388881 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8555 0.8410292507122451 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3530 0.34702901870417596 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3512 0.34525946563429627 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3486 0.342703444533359 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3154 0.31006502124446766 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3030 0.2978747667630745 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2310 0.22709264396788853 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1689 0.16604306305704056 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1598 0.1570969892037601 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1520 0.14942892590094828 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1466 0.14412026669130934 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1347 0.1324215547293272 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1340 0.13173339520215177 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1273 0.12514672544204417 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1241 0.12200085331781368 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1069 0.10509179065007482 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.915425194110141E-5 0.0 16-17 0.0 0.0 0.0 1.9661700776440563E-4 0.0 18-19 0.0 0.0 0.0 2.4577125970550703E-4 0.0 20-21 0.0 0.0 0.0 4.915425194110142E-4 0.0 22-23 0.0 0.0 0.0 0.0012288562985275351 0.0 24-25 0.0 0.0 0.0 0.0019661700776440563 0.0 26-27 0.0 0.0 0.0 0.002900100864524983 0.0 28-29 0.0 0.0 0.0 0.005112042201874547 0.0 30-31 0.0 0.0 0.0 0.011551249206158833 0.0 32-33 0.0 0.0 0.0 0.020988865578850303 0.0 34-35 0.0 0.0 0.0 0.03607922092476844 0.0 36-37 0.0 0.0 0.0 0.062524208469081 0.0 38-39 0.0 0.0 0.0 0.09535924876573673 0.0 40-41 0.0 0.0 0.0 0.1370912086637318 0.0 42-43 0.0 0.0 0.0 0.17607053045302523 0.0 44-45 0.0 0.0 0.0 0.22242299003348387 0.0 46-47 0.0 4.915425194110141E-5 0.0 0.2860285920452691 0.0 48-49 0.0 9.830850388220282E-5 0.0 0.35759718287151276 0.0 50-51 0.0 9.830850388220282E-5 0.0 0.42936239070552085 0.0 52-53 0.0 9.830850388220282E-5 0.0 0.5042243164118183 0.0 54-55 0.0 9.830850388220282E-5 0.0 0.5846898268394012 0.0 56-57 0.0 9.830850388220282E-5 0.0 0.679557533085727 0.0 58-59 0.0 9.830850388220282E-5 0.0 0.7747693190956404 0.0 60-61 0.0 9.830850388220282E-5 0.0 0.8847273806878843 0.0 62-63 0.0 9.830850388220282E-5 0.0 0.9854935971671421 0.0 64-65 0.0 9.830850388220282E-5 0.0 1.0865547391580468 0.0 66-67 0.0 9.830850388220282E-5 0.0 1.1951856359478807 0.0 68-69 0.0 9.830850388220282E-5 0.0 1.3226917654830976 0.0 70-71 0.0 9.830850388220282E-5 0.0 1.4811159194892678 0.0 72-73 0.0 9.830850388220282E-5 0.0 1.6724734222959754 0.0 74-75 0.0 9.830850388220282E-5 0.0 1.863732616598801 0.0 76-77 0.0 9.830850388220282E-5 0.0 2.0773078412828867 0.0 78-79 0.0 9.830850388220282E-5 0.0 2.3271097496475637 0.0 80-81 0.0 9.830850388220282E-5 0.0 2.6040939593356702 0.0 82-83 0.0 9.830850388220282E-5 0.0 2.895775290354166 0.0 84-85 0.0 9.830850388220282E-5 0.0 3.1971891632569998 0.0 86-87 0.0 9.830850388220282E-5 0.0 3.512415380955283 0.0 88-89 0.0 9.830850388220282E-5 0.0 3.8415030977009574 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACCG 40 1.0934007E-5 35.551407 18-19 CGACCGT 55 9.6143514E-5 25.85557 18-19 GGTATCA 12940 0.0 24.460033 1 CGACGAA 50 0.0016569385 23.742958 60-61 CGTCACT 225 2.8448994E-9 23.199343 1 GAGTACG 125 2.2707577E-4 22.777536 1 GAGTACT 9865 0.0 22.750822 12-13 GTATCAA 16805 0.0 22.138237 1 GTACATG 9430 0.0 21.889864 1 GTACTTT 10435 0.0 21.59893 14-15 TACATGG 9430 0.0 21.533375 2 AGTACTT 10010 0.0 21.142756 12-13 GTACACG 115 0.0037443086 20.631824 1 GTATTAG 210 8.6874934E-7 20.337086 1 ACATGGG 9590 0.0 20.08177 3 CGGGAGT 430 0.0 19.859243 4 TACACGA 120 0.0047958205 19.768274 2 ACTTTTT 11790 0.0 19.699072 16-17 GTAGCCC 315 3.274181E-10 19.576153 3 GCACCGT 170 8.0770085E-5 19.51938 6 >>END_MODULE