FastQCFastQC Report
Fri 27 May 2016
SRR939459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870189
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104490.5587135845628436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85650.457975103051082No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53640.2868159314379456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23740.12693904198987374No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.11886499172008819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82000.044.034771
GTATCAA133900.036.44261
TATCAAC166750.028.9430852
ATCAACG173100.028.2204273
TCAACGC177000.027.6595044
CAACGCA177050.027.449865
AACGCAG180050.027.1253456
GTACATG115800.025.0753561
TACATGG118550.024.6407182
GAGTACT106700.024.29837212-13
ACGCAGA199200.023.7257257
CGCAGAG200150.023.4953148
ACATGGG121950.022.6033333
GCAGAGT197350.022.2871679
GTACTTT117400.022.04678514-15
AGTACTT110000.021.69606612-13
CATGGGA75900.021.3956094
AACGCGG3051.6370905E-1020.6116855
ATCGACG1404.5841688E-420.5300454
CAACGCG3102.3101165E-1020.0885414
Produced by FastQC (version 0.11.5)