FastQCFastQC Report
Fri 27 May 2016
SRR939459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870189
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149870.8013628569091146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143160.7654841302135774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91040.4867957195770053No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37810.20217207993416708No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36340.19431191179073345No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.14180384977133328No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.10704800423914375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88150.045.5464481
GTATCAA144650.035.555331
GATACGC550.002609473234.465632
TATCAAC183000.027.9939042
ATCAACG183000.027.8873373
TCAACGC185250.027.5742054
CAACGCA189550.026.9236765
AACGCAG193700.026.3468426
GTACATG135100.023.963971
TACATGG135500.023.6426582
GAGTACT124400.023.23549312-13
ACGCAGA221550.022.9279637
CGCAGAG222450.022.7712968
GACCGTA1252.2939223E-422.744887
ACCGTAT1252.2939223E-422.744888
GTACTTT129300.022.3366314-15
ACATGGG136350.022.1374053
GCAGAGT230300.021.5218779
AGTACTT126200.020.98915312-13
GTGTAGC9100.020.8358881
Produced by FastQC (version 0.11.5)