##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939459_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1870189 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.995884373183674 31.0 31.0 31.0 30.0 34.0 2 31.252927377928113 31.0 31.0 34.0 30.0 34.0 3 31.230984675880354 31.0 31.0 34.0 28.0 34.0 4 35.177751553452616 35.0 35.0 37.0 33.0 37.0 5 34.81702277149529 35.0 35.0 37.0 32.0 37.0 6 34.79075804637927 35.0 35.0 37.0 32.0 37.0 7 34.649947144379524 35.0 35.0 37.0 30.0 37.0 8 34.67313731392923 35.0 35.0 37.0 30.0 37.0 9 35.99942358766948 37.0 35.0 39.0 30.0 39.0 10-11 35.863793445475295 37.0 35.0 39.0 30.0 39.0 12-13 35.825127834673395 37.0 35.0 39.0 30.0 39.0 14-15 36.64153248682352 38.0 35.5 40.0 30.5 41.0 16-17 36.6661422989869 38.0 35.5 40.0 31.0 41.0 18-19 36.676401155177366 38.0 35.0 40.0 30.0 41.0 20-21 36.622036328948575 38.0 35.0 40.0 30.0 41.0 22-23 36.48889711146842 38.0 35.0 40.0 30.0 41.0 24-25 36.27644959947898 38.0 34.5 40.0 29.5 41.0 26-27 35.90162999568493 38.0 34.0 40.0 28.5 41.0 28-29 35.612827901351146 38.0 34.0 40.0 27.0 41.0 30-31 35.35131422546064 38.0 34.0 40.0 27.0 41.0 32-33 35.107289156336606 37.5 33.0 40.0 27.0 41.0 34-35 35.00827884240577 37.5 33.0 40.0 26.5 41.0 36-37 34.95997276211121 38.0 33.0 40.0 25.5 41.0 38-39 34.90903726842581 38.0 33.0 40.0 26.0 41.0 40-41 34.65352084735821 38.0 33.0 40.0 25.0 41.0 42-43 34.311148231542376 37.0 33.0 40.0 24.0 41.0 44-45 34.04669153759326 37.0 33.0 40.0 24.0 41.0 46-47 33.61554500641379 37.0 32.5 40.0 22.5 41.0 48-49 33.21866854098703 37.0 32.0 40.0 20.0 41.0 50-51 32.92609009035985 37.0 31.0 40.0 18.5 41.0 52-53 32.5623268557349 36.5 31.0 40.0 15.5 41.0 54-55 32.33476803681339 36.0 31.0 39.0 14.5 41.0 56-57 31.960815725041694 36.0 30.5 39.0 13.0 40.0 58-59 31.55209767568946 35.5 30.0 39.0 8.5 40.0 60-61 31.283328583367776 35.0 29.0 39.0 8.0 40.0 62-63 30.90263283550486 35.0 29.0 38.0 8.0 40.0 64-65 30.476627495937578 34.5 28.0 38.0 6.5 40.0 66-67 28.84669597564738 33.5 25.0 37.5 2.0 39.5 68-69 25.302203948370995 30.0 15.5 35.0 2.0 39.0 70-71 24.72224571955027 29.5 13.0 35.0 2.0 38.0 72-73 26.48686523126807 31.0 21.0 35.0 2.0 38.0 74-75 27.06228541607292 32.0 23.5 35.0 2.0 38.0 76-77 27.075298271992832 31.5 25.0 34.5 2.0 36.5 78-79 27.88268084134812 33.0 26.0 35.0 2.0 37.0 80-81 27.735722164979045 33.0 26.0 35.0 2.0 37.0 82-83 27.34389679331875 33.0 26.0 35.0 2.0 36.0 84-85 27.01014737013211 32.5 25.5 35.0 2.0 36.0 86-87 26.55696616759055 32.0 25.0 35.0 2.0 35.5 88-89 26.080574744049933 32.0 24.0 34.0 2.0 35.0 90-91 25.646722336619455 32.0 22.5 34.0 2.0 35.0 92-93 25.11507419838316 31.5 19.5 34.0 2.0 35.0 94-95 23.791455836816496 31.0 7.0 34.0 2.0 35.0 96-97 23.010071709329914 30.0 2.0 34.0 2.0 35.0 98-99 22.686509491821415 30.0 2.0 34.0 2.0 35.0 100-101 21.48256593317574 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.274046442720671 1101 2 0.18091295139884522 1101 3 0.2650959184441142 1101 4 0.26554348338895295 1101 5 0.35046593650961455 1101 6 0.5138373146101074 1101 7 0.5797447183738882 1101 8 0.5240005112608017 1101 9 0.6344144386394248 1101 10-11 0.64086656177912 1101 12-13 0.6355796869412629 1101 14-15 0.7325171320778878 1101 16-17 0.6498098843318942 1101 18-19 0.6596525302149985 1101 20-21 0.7356099118958923 1101 22-23 0.8019267459683235 1101 24-25 0.6554481271732371 1101 26-27 0.7007441444807867 1101 28-29 0.6045833168740771 1101 30-31 0.6237229283195589 1101 32-33 0.7210034227634097 1101 34-35 0.612688237113673 1101 36-37 0.6754658428770952 1101 38-39 0.788243804650655 1101 40-41 0.7732312491494326 1101 42-43 0.6601819157188231 1101 44-45 0.41785421555144353 1101 46-47 0.5780649501697326 1101 48-49 0.6170153369854958 1101 50-51 0.5838420692605055 1101 52-53 0.5982334997276268 1101 54-55 0.7198829391398078 1101 56-57 0.5165289694729509 1101 58-59 0.5731190948486926 1101 60-61 0.2369453741609142 1101 62-63 0.2424258863883324 1101 64-65 -0.06786344893361829 1101 66-67 -2.936218645984532 1101 68-69 -7.927205532235334 1101 70-71 -11.423399250420202 1101 72-73 -11.523033871978683 1101 74-75 -10.118347781424092 1101 76-77 -4.96286288377566 1101 78-79 -1.7358261312480359 1101 80-81 -1.092271680525382 1101 82-83 -0.9451562961077151 1101 84-85 -1.1730316723670136 1101 86-87 -0.8652658087807623 1101 88-89 -0.7489373849883911 1101 90-91 -1.008956456092136 1101 92-93 -1.7200048710888751 1101 94-95 -5.127035794242886 1101 96-97 -2.0016256976051245 1101 98-99 -1.305828837755353 1101 100-101 -1.0591054433377067 1102 1 0.3741026131919085 1102 2 0.2566954188403656 1102 3 0.372562119103911 1102 4 0.18495409149942077 1102 5 0.3188841750567093 1102 6 0.4071089963874357 1102 7 0.41303071059847696 1102 8 0.44550829772651923 1102 9 0.6475498763096112 1102 10-11 0.6820971865294112 1102 12-13 0.6279459331566386 1102 14-15 0.5362397895161521 1102 16-17 0.6769319167692416 1102 18-19 0.6041212868032346 1102 20-21 0.7674145369420273 1102 22-23 0.6852484405965527 1102 24-25 0.6759316748274742 1102 26-27 0.6690982513874957 1102 28-29 0.751013911941989 1102 30-31 0.504394094193465 1102 32-33 0.24509910520514921 1102 34-35 0.6288699277023468 1102 36-37 0.8263738584475675 1102 38-39 0.8134235821015778 1102 40-41 0.6455930675787442 1102 42-43 0.9884972143830737 1102 44-45 0.8039388535909922 1102 46-47 0.9299413780815939 1102 48-49 0.7501237509092888 1102 50-51 0.8043710004932052 1102 52-53 0.9248855528984166 1102 54-55 0.7385953723728775 1102 56-57 0.6865603767766828 1102 58-59 0.7839667283911638 1102 60-61 0.6893942199633862 1102 62-63 0.6071097223155171 1102 64-65 0.40251105036044166 1102 66-67 -2.7277818461189867 1102 68-69 -7.458820096653728 1102 70-71 -10.765780264112923 1102 72-73 -8.785637090748171 1102 74-75 -7.477394667304658 1102 76-77 -3.943386030874965 1102 78-79 -1.1718234912850285 1102 80-81 -0.5060742101131659 1102 82-83 -0.30289388909245574 1102 84-85 -0.4639405331974231 1102 86-87 -0.4250933806684003 1102 88-89 -0.29728588467657957 1102 90-91 -0.3431815714185191 1102 92-93 -0.5173476588839101 1102 94-95 -4.121014791499231 1102 96-97 -0.9707601956607803 1102 98-99 -0.25541841982837 1102 100-101 0.014647908830507816 1103 1 0.3504696842488535 1103 2 0.3768741943888756 1103 3 0.4473829350603218 1103 4 0.2915736887351841 1103 5 0.5523828198772662 1103 6 0.4827918203246284 1103 7 0.5615547571435755 1103 8 0.5108770377776395 1103 9 0.7716106651409973 1103 10-11 0.759026218191238 1103 12-13 0.579393018038111 1103 14-15 0.9057923051908077 1103 16-17 0.8075114771999523 1103 18-19 0.6659530311343858 1103 20-21 0.8134555050682124 1103 22-23 0.8209241855403207 1103 24-25 0.8062961500947026 1103 26-27 0.809985821870761 1103 28-29 0.9879601123065598 1103 30-31 0.8243232709127284 1103 32-33 0.8773744561357617 1103 34-35 0.9712323916970149 1103 36-37 0.7821715945511727 1103 38-39 0.8017699201873469 1103 40-41 0.9318168747671649 1103 42-43 0.9564951383229499 1103 44-45 0.9438828361726692 1103 46-47 0.8836258964992467 1103 48-49 0.8065237016516846 1103 50-51 0.8427894266462275 1103 52-53 0.9774668487781781 1103 54-55 0.977752805423151 1103 56-57 1.0100089231892326 1103 58-59 1.0571323889848685 1103 60-61 0.92449623773852 1103 62-63 0.8564645263724557 1103 64-65 0.8352868137162694 1103 66-67 -2.0743207256801064 1103 68-69 -6.817761863358019 1103 70-71 -11.073973603523264 1103 72-73 -8.810576251182411 1103 74-75 -7.076413397816289 1103 76-77 -3.4820770649677506 1103 78-79 -0.6123541109089423 1103 80-81 0.004088742503551401 1103 82-83 0.2094587400557053 1103 84-85 0.012717724584170753 1103 86-87 0.2831655085060518 1103 88-89 0.46950927731256087 1103 90-91 0.5132131153348354 1103 92-93 0.5886378777702461 1103 94-95 1.3058679242046267 1103 96-97 1.2865680035762033 1103 98-99 1.067560654981488 1103 100-101 1.058980462410652 1104 1 0.33317370402264856 1104 2 0.2482334671630042 1104 3 0.38975543917498356 1104 4 0.17624208521854 1104 5 0.4867886395585188 1104 6 0.5859374704994593 1104 7 0.6549426011789308 1104 8 0.3861034465727613 1104 9 0.5893138356255605 1104 10-11 0.7621050966608749 1104 12-13 0.7623828675674886 1104 14-15 0.8837627967828396 1104 16-17 0.8136329197030747 1104 18-19 0.8255486523162432 1104 20-21 0.8574450558820672 1104 22-23 0.712955978243464 1104 24-25 0.8111554902262483 1104 26-27 0.9301342424856429 1104 28-29 0.8612411216136024 1104 30-31 1.0704846592956159 1104 32-33 0.8335952947649545 1104 34-35 0.8061634821258252 1104 36-37 0.7734998243395523 1104 38-39 0.7383924433378013 1104 40-41 0.8217954961071712 1104 42-43 0.9017754916272764 1104 44-45 0.6218726564162154 1104 46-47 0.7241819759458608 1104 48-49 0.8514641049762091 1104 50-51 0.879428467007962 1104 52-53 0.8540852719116572 1104 54-55 0.9841132203448737 1104 56-57 0.8962394261441702 1104 58-59 0.9388360046141315 1104 60-61 0.9146823199379988 1104 62-63 0.8907355140665487 1104 64-65 0.9361999460706762 1104 66-67 -2.357126308731811 1104 68-69 -6.862514954477966 1104 70-71 -8.806124413862335 1104 72-73 -8.0358236872075 1104 74-75 -6.5299600882240085 1104 76-77 -3.3305539086575315 1104 78-79 -0.2762159754795235 1104 80-81 0.39608773507080386 1104 82-83 0.553218104201644 1104 84-85 0.5698102268245364 1104 86-87 0.7554852395449387 1104 88-89 0.6579888152992197 1104 90-91 0.49692480214039136 1104 92-93 0.6534480176979649 1104 94-95 1.5808021775036778 1104 96-97 1.5786379850089851 1104 98-99 1.14212707818524 1104 100-101 1.2126759248632837 1105 1 0.5124193671973387 1105 2 0.45068456687359415 1105 3 0.5548174270976745 1105 4 0.3647771437707803 1105 5 0.44311653208561097 1105 6 0.5022947119349936 1105 7 0.5527839423774594 1105 8 0.4978578008676564 1105 9 0.6721210820108112 1105 10-11 0.8352751863963235 1105 12-13 0.779394286855073 1105 14-15 1.0238167195789103 1105 16-17 1.097330066919291 1105 18-19 1.0114213456026846 1105 20-21 1.1169123499010638 1105 22-23 1.0972947252081227 1105 24-25 1.012944492538061 1105 26-27 1.0345090932662444 1105 28-29 1.0218921549397564 1105 30-31 1.0113008384637112 1105 32-33 1.0453809778549754 1105 34-35 1.2297250653363818 1105 36-37 1.2646932883842297 1105 38-39 1.2281407065695547 1105 40-41 1.1445462574900631 1105 42-43 1.378988044108823 1105 44-45 1.2424421649966888 1105 46-47 1.2553526130617456 1105 48-49 1.1295465264940887 1105 50-51 1.1509982259858802 1105 52-53 1.3464802942436052 1105 54-55 1.1229752513592146 1105 56-57 1.2843534676169668 1105 58-59 1.2797149578364042 1105 60-61 1.3320638615709477 1105 62-63 1.5333977628684217 1105 64-65 1.3099588773977224 1105 66-67 2.0645316716673143 1105 68-69 -4.891263607863863 1105 70-71 -5.045767684868061 1105 72-73 -1.1578095135481483 1105 74-75 1.2952284959823217 1105 76-77 1.4764892470489066 1105 78-79 1.349781101276026 1105 80-81 1.201977548553831 1105 82-83 1.3677640987161936 1105 84-85 1.1459437410651638 1105 86-87 1.340701658628717 1105 88-89 1.3672360507368566 1105 90-91 1.3487864197078103 1105 92-93 1.388293440956975 1105 94-95 2.160786105482387 1105 96-97 2.346514905935802 1105 98-99 2.045883622001881 1105 100-101 1.9437615433158264 1106 1 0.23233648055414946 1106 2 0.24512893430136984 1106 3 0.20625507895920592 1106 4 0.06746090312507391 1106 5 0.26507120101484105 1106 6 0.2591651795856862 1106 7 0.23292082423157012 1106 8 0.23474675095216213 1106 9 0.31852364520906207 1106 10-11 0.2971885905782088 1106 12-13 0.24200847744501175 1106 14-15 0.4777417461242024 1106 16-17 0.2603554246963071 1106 18-19 0.24738229705562986 1106 20-21 0.34918715309932935 1106 22-23 0.2861648272515538 1106 24-25 0.4402290621024818 1106 26-27 0.43468198132627833 1106 28-29 0.5424083378777809 1106 30-31 0.34425521063076303 1106 32-33 0.361331675387099 1106 34-35 0.5149854301024632 1106 36-37 0.562677185217197 1106 38-39 0.38319918610859105 1106 40-41 0.26482611151972435 1106 42-43 0.3004426346768625 1106 44-45 0.3828716548406277 1106 46-47 0.2922306607189782 1106 48-49 0.3330185971305397 1106 50-51 0.01780158026800649 1106 52-53 0.2854396246654005 1106 54-55 0.3219433742126512 1106 56-57 0.4305242367526745 1106 58-59 0.5408465358006183 1106 60-61 0.4065088775051713 1106 62-63 0.3365325866544211 1106 64-65 0.3430651316550133 1106 66-67 1.046729347241623 1106 68-69 -5.64493736165651 1106 70-71 -6.035082013829538 1106 72-73 -2.3567600139437097 1106 74-75 0.2638880120038394 1106 76-77 0.4583701883137259 1106 78-79 0.3234747660964068 1106 80-81 0.36553745421964123 1106 82-83 0.35068382513106044 1106 84-85 0.4157318227854354 1106 86-87 0.419042999998517 1106 88-89 0.3844339248575288 1106 90-91 0.28302351883507626 1106 92-93 0.2889295646929462 1106 94-95 1.2871513466645226 1106 96-97 1.4258485091668724 1106 98-99 1.2279884624681152 1106 100-101 1.1037057905584362 1107 1 -0.06699707618556161 1107 2 -0.033718807956891084 1107 3 -0.07879643823757831 1107 4 -0.16395325926619364 1107 5 0.10771389397423548 1107 6 0.10625680609426524 1107 7 -0.06200674962233421 1107 8 -0.06751335071347597 1107 9 0.1360713511244498 1107 10-11 0.09849941178544697 1107 12-13 0.0631069288612025 1107 14-15 0.0788739208909206 1107 16-17 -0.010554704580464147 1107 18-19 0.08158093679396217 1107 20-21 -0.017144954645480937 1107 22-23 0.025452176629237044 1107 24-25 0.2686815166620917 1107 26-27 0.17728420154518432 1107 28-29 0.11559426233181824 1107 30-31 0.08583499266794092 1107 32-33 -0.11181177038886148 1107 34-35 0.19396556424068478 1107 36-37 0.255270791704163 1107 38-39 0.24723850720491924 1107 40-41 0.267998560793103 1107 42-43 0.1924948740111745 1107 44-45 0.09937060939316211 1107 46-47 0.12914805384970407 1107 48-49 0.23089678716126372 1107 50-51 0.14420738851925563 1107 52-53 0.38948005492721194 1107 54-55 0.2905519851126783 1107 56-57 0.2665160666869184 1107 58-59 0.1428148325472236 1107 60-61 0.19521922483916043 1107 62-63 0.3045318645168926 1107 64-65 0.11460677727900404 1107 66-67 0.96339597457618 1107 68-69 -5.732273405758818 1107 70-71 -6.134913161084235 1107 72-73 -2.5453973215506984 1107 74-75 -0.09389797569622615 1107 76-77 -0.11274773249657599 1107 78-79 -0.0611820533833729 1107 80-81 -0.09640495413261263 1107 82-83 0.05030021906888038 1107 84-85 -0.0986118108406906 1107 86-87 0.13280656367641797 1107 88-89 -0.05350083261281924 1107 90-91 -0.0014954809336487074 1107 92-93 0.1335392564393416 1107 94-95 0.931635608314302 1107 96-97 0.9620570983555758 1107 98-99 0.7208555844432816 1107 100-101 0.5751955177981287 1108 1 0.12059669076731439 1108 2 -0.03089878438654381 1108 3 0.020494580712231425 1108 4 0.00892681256723904 1108 5 0.1480247980653786 1108 6 0.2519664418762986 1108 7 0.2622295524522329 1108 8 0.03830160336176647 1108 9 0.28654182271844064 1108 10-11 0.35865383496669523 1108 12-13 0.26862912427059626 1108 14-15 0.17930136724968548 1108 16-17 0.061026192215088315 1108 18-19 0.13892609271367462 1108 20-21 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2.028623583958858 2316 100-101 2.0432838451097552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 21.0 4 63.0 5 135.0 6 384.0 7 859.0 8 1837.0 9 3290.0 10 5525.0 11 8095.0 12 10746.0 13 13572.0 14 17002.0 15 21895.0 16 26343.0 17 29558.0 18 25845.0 19 19862.0 20 19002.0 21 18975.0 22 19926.0 23 22452.0 24 27572.0 25 33912.0 26 42846.0 27 52807.0 28 65171.0 29 77998.0 30 91421.0 31 106507.0 32 123626.0 33 143852.0 34 167924.0 35 194863.0 36 208021.0 37 181761.0 38 80966.0 39 5553.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.58095295144535 20.524215286929437 15.67821629670198 21.21661546492324 2 15.958678574350918 21.822316372245805 39.18540144580066 23.033603607602622 3 19.30163208103566 26.388242043985926 30.91051225303967 23.399613621938745 4 12.860518375415534 18.173724687718728 36.692013480990425 32.27374345587531 5 12.802930612895274 38.851527840234326 33.622002909866325 14.723538637004069 6 30.3354366858109 37.17159067880305 17.330227051918282 15.162745583467768 7 27.242487256635563 34.81145488504103 20.17100945412469 17.77504840419872 8 26.873112824425764 34.89513626697622 19.159453937543212 19.072296971054797 9 26.966258490452034 16.674571393586422 20.477983775971307 35.881186339990236 10-11 25.01183035511384 27.825128904084025 26.12586214548369 21.03717859531844 12-13 26.221360514899832 25.465474345106298 27.42578423891917 20.887380901074703 14-15 23.07018167682518 26.132439020869015 27.013873998831134 23.783505303474676 16-17 21.031839923729407 29.60746899969976 27.656911741548356 21.70377933502247 18-19 20.737074001491848 26.974625233602385 31.699636128363007 20.588664636542763 20-21 22.085922866619363 25.893024715683815 31.8136830020923 20.20736941560452 22-23 21.62225315195416 26.167515689590733 31.5903098563835 20.61992130207161 24-25 21.199354717624797 26.35033143709005 31.92257573967123 20.527738105613924 26-27 21.06062404621582 26.57139786395121 31.746001191327906 20.621976898505064 28-29 20.672818135557826 26.58991903902529 32.08950015549363 20.647762669923253 30-31 20.865867000621353 26.524826828738785 31.979414089194563 20.629892081445302 32-33 20.773505243588993 26.63758716031241 31.675860293238856 20.913047302859745 34-35 21.23792556799663 26.6267846442984 31.54030006703153 20.594989720673436 36-37 21.1789481459358 26.444882275393528 31.480821345168685 20.89534823350199 38-39 20.9915752687208 26.72873724842426 31.659475889060857 20.62021159379408 40-41 21.107000620187158 26.554904497333894 31.67122104064568 20.66687384183327 42-43 20.958490566037735 26.687943262411345 31.261099959855482 21.092466211695438 44-45 21.070637863766144 27.088877988025285 30.857142398373753 20.983341749834814 46-47 21.125230988678375 27.289648094351765 30.19475975170306 21.390361165266796 48-49 21.514259188834263 27.39850124699825 29.79762472190427 21.28961484226322 50-51 21.455931929871326 27.660233842537245 29.522747447758224 21.3610867798332 52-53 21.688490952381844 27.86416492508501 29.282384522500713 21.16495960003243 54-55 21.265924717087135 27.755183391744026 29.436394767115225 21.542497124053614 56-57 21.40533346741711 28.248179438324495 29.057763718000423 21.28872337625797 58-59 21.535572812854802 27.682474012885393 29.241166092384013 21.54078708187579 60-61 21.498051613655335 27.732139257083787 29.526542385624882 21.243266743635996 62-63 21.583578010545672 27.604696091385133 29.476389874400517 21.335336023668678 64-65 21.34706068409135 27.537095209232298 29.420510762470077 21.695333344206272 66-67 20.884105169048535 27.933615595475576 29.64399530696679 21.538283928509102 68-69 20.97364760459844 27.614910518614426 29.945774002732456 21.46566787405467 70-71 20.492425274460555 27.669066001906124 30.2240167855454 21.614491938087927 72-73 21.296753799137853 27.797225247996575 29.110552813666306 21.795468139199265 74-75 20.985228639201743 28.007990945522927 29.13353506159888 21.873245353676456 76-77 21.173662634629633 28.051495843223577 28.949565979115977 21.825275543030813 78-79 21.11546104418358 28.177994198980116 28.82015447996167 21.886390276874636 80-81 20.88284448465492 28.424393748233772 28.754018039637796 21.93874372747351 82-83 21.157353615062434 28.43541481636348 28.414267221120433 21.992964347453654 84-85 21.018383703465265 28.196882774949483 28.37058179681305 22.414151724772204 86-87 21.155786525475342 28.119417318430678 28.620411607218767 22.104384548875217 88-89 21.124980583362678 28.116493942142835 28.42117139336463 22.337354081129863 90-91 20.8529699493269 28.347096677253308 28.623174553351927 22.176758820067864 92-93 20.898816864987644 28.265732556892903 28.575064626875207 22.26038595124424 94-95 20.464170352100126 28.057319934030627 28.75093615145624 22.727573562413 96-97 20.57710055232985 28.003755800106546 28.618977080401347 22.800166567162258 98-99 20.670794691592043 27.974494913234732 28.259082096035538 23.095628299137683 100-101 20.51859165993555 28.016992099924842 28.550936327485083 22.913479912654534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3396.0 1 2271.0 2 1187.5 3 1269.5 4 1192.0 5 1195.5 6 1861.5 7 3455.0 8 5089.5 9 6022.0 10 5841.0 11 4972.5 12 4578.5 13 4611.5 14 4787.0 15 4924.0 16 5020.0 17 5224.0 18 5585.0 19 5982.0 20 6456.5 21 7152.5 22 8020.0 23 9091.0 24 10313.5 25 12039.5 26 14576.5 27 17314.5 28 19652.5 29 22090.0 30 24775.0 31 28216.5 32 32645.0 33 36830.0 34 40746.0 35 44470.5 36 48821.5 37 52620.0 38 53652.5 39 53507.0 40 53791.0 41 55463.5 42 57197.0 43 59085.0 44 61472.5 45 62587.0 46 63313.5 47 64655.5 48 69379.0 49 70637.5 50 66498.5 51 63367.0 52 60317.0 53 58562.0 54 56408.5 55 51874.0 56 47006.5 57 42013.5 58 37233.0 59 32720.0 60 27689.0 61 22325.5 62 17313.5 63 13265.0 64 9906.0 65 7265.5 66 5266.0 67 3961.0 68 2822.5 69 1830.5 70 1334.5 71 987.0 72 774.0 73 647.5 74 494.5 75 375.0 76 257.0 77 145.5 78 80.0 79 42.0 80 26.5 81 16.5 82 7.5 83 5.0 84 6.0 85 4.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024649915062060573 2 0.0192493913716742 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.940879237339114E-4 18-19 0.0012832927581116133 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.742937211158872E-4 28-29 0.018046304410944562 30-31 0.004010289869098791 32-33 0.007673021282875688 34-35 0.009277137230515205 36-37 0.10095236363811358 38-39 0.10370609601489475 40-41 0.11787578688571049 42-43 0.10367936074910077 44-45 0.09814516072974443 46-47 0.099535394551032 48-49 0.10172768634613934 50-51 0.1005513346512037 52-53 0.07686388915772684 54-55 0.06624998863751204 56-57 0.03801754795905655 58-59 0.01711057010815484 60-61 0.0096246956858371 62-63 0.0020318802003433875 64-65 0.0054807294877683484 66-67 0.4199575550920254 68-69 0.8064692926757671 70-71 0.892396436937657 72-73 0.2776992058021943 74-75 0.15108098700184847 76-77 0.015533189426309319 78-79 0.009250401964721213 80-81 0.0015773806818455247 82-83 0.0 84-85 0.0 86-87 4.2776425270387114E-4 88-89 7.753227080257663E-4 90-91 0.0034488492874249605 92-93 0.0016308512134335087 94-95 0.22305232251927481 96-97 0.22858652253863113 98-99 0.015372777831545368 100-101 0.007673021282875688 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1870189.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.6653427655943 #Duplication Level Percentage of deduplicated Percentage of total 1 76.15191149706591 40.10566521248178 2 13.464300464156654 14.18203998087521 3 4.52078298765305 7.14265756840846 4 2.0031859025560577 4.219938887252846 5 1.0281024572284596 2.707268415404329 6 0.6264283135834665 1.9794637111767872 7 0.3980787740771046 1.4675468559134803 8 0.27973164233382725 1.1785730260714915 9 0.2001569516999602 0.9487201021375414 >10 1.146485071358051 11.496917330199658 >50 0.11118020457387102 4.071386626641818 >100 0.06462228284975702 6.109254834649307 >500 0.0038007690196034897 1.3216009981446204 >1k 9.245113831467947E-4 0.991160173851062 >5k 1.0272348701631053E-4 0.49252345520113905 >10k+ 2.0544697403262105E-4 1.5852828215903971 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14987 0.8013628569091146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14316 0.7654841302135774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9104 0.4867957195770053 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3781 0.20217207993416708 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3634 0.19431191179073345 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.14180384977133328 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2002 0.10704800423914375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.3470531587983886E-5 0.0 0.0 0.0 0.0 8 5.3470531587983886E-5 0.0 0.0 0.0 0.0 9 5.3470531587983886E-5 0.0 0.0 0.0 0.0 10-11 5.3470531587983886E-5 0.0 0.0 0.0 0.0 12-13 5.3470531587983886E-5 0.0 0.0 0.0 0.0 14-15 5.3470531587983886E-5 0.0 0.0 0.0 0.0 16-17 5.3470531587983886E-5 0.0 0.0 0.0 0.0 18-19 5.3470531587983886E-5 0.0 0.0 0.0 0.0 20-21 5.3470531587983886E-5 0.0 0.0 8.020579738197583E-5 0.0 22-23 1.6041159476395166E-4 0.0 0.0 2.6735265793991945E-4 0.0 24-25 2.1388212635193554E-4 0.0 0.0 5.881758474678227E-4 0.0 26-27 2.1388212635193554E-4 0.0 0.0 0.0010159401001716938 0.0 28-29 2.6735265793991945E-4 0.0 0.0 0.002539850250429235 0.0 30-31 2.6735265793991945E-4 0.0 0.0 0.006362993258970082 0.0 32-33 2.6735265793991945E-4 0.0 0.0 0.013528044491759924 0.0 34-35 3.2082318952790333E-4 0.0 0.0 0.026173825212318112 0.0 36-37 3.2082318952790333E-4 0.0 0.0 0.05042271128746881 0.0 38-39 3.742937211158872E-4 0.0 0.0 0.08509835102227636 0.0 40-41 3.742937211158872E-4 0.0 0.0 0.12573595502914411 0.0 42-43 3.742937211158872E-4 0.0 0.0 0.16573191265695608 0.0 44-45 3.742937211158872E-4 0.0 0.0 0.2134543620992317 0.0 46-47 4.277642527038711E-4 0.0 0.0 0.2711223304168723 0.0 48-49 4.277642527038711E-4 0.0 0.0 0.33924378765996377 0.0 50-51 4.277642527038711E-4 0.0 0.0 0.41236473960653175 0.0 52-53 4.277642527038711E-4 0.0 0.0 0.48059313791279923 0.0 54-55 4.277642527038711E-4 0.0 0.0 0.5586333787654617 0.0 56-57 4.81234784291855E-4 0.0 0.0 0.6479024312516009 0.0 58-59 4.81234784291855E-4 0.0 0.0 0.7394172460644353 0.0 60-61 4.81234784291855E-4 0.0 0.0 0.8326698531538791 0.0 62-63 4.81234784291855E-4 0.0 0.0 0.9303337790993317 0.0 64-65 4.81234784291855E-4 0.0 0.0 1.0301632615740974 0.0 66-67 4.81234784291855E-4 0.0 0.0 1.1342703865759023 0.0 68-69 4.81234784291855E-4 0.0 0.0 1.2601934884656043 0.0 70-71 4.81234784291855E-4 0.0 0.0 1.4320745122551786 0.0 72-73 4.81234784291855E-4 0.0 0.0 1.6193283138762982 0.0 74-75 4.81234784291855E-4 0.0 0.0 1.8192813667495638 0.0 76-77 4.81234784291855E-4 0.0 0.0 2.0396066921578515 0.0 78-79 4.81234784291855E-4 0.0 0.0 2.2975485365382857 0.0 80-81 5.347053158798389E-4 0.0 0.0 2.580835412891424 0.0 82-83 5.347053158798389E-4 0.0 0.0 2.880671418771044 0.0 84-85 5.347053158798389E-4 0.0 0.0 3.1680220555248697 0.0 86-87 5.347053158798389E-4 0.0 0.0 3.4642488005223004 0.0 88-89 5.347053158798389E-4 5.3470531587983886E-5 0.0 3.7992416809210194 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8815 0.0 45.546448 1 GTATCAA 14465 0.0 35.55533 1 GATACGC 55 0.0026094732 34.46563 2 TATCAAC 18300 0.0 27.993904 2 ATCAACG 18300 0.0 27.887337 3 TCAACGC 18525 0.0 27.574205 4 CAACGCA 18955 0.0 26.923676 5 AACGCAG 19370 0.0 26.346842 6 GTACATG 13510 0.0 23.96397 1 TACATGG 13550 0.0 23.642658 2 GAGTACT 12440 0.0 23.235493 12-13 ACGCAGA 22155 0.0 22.927963 7 CGCAGAG 22245 0.0 22.771296 8 GACCGTA 125 2.2939223E-4 22.74488 7 ACCGTAT 125 2.2939223E-4 22.74488 8 GTACTTT 12930 0.0 22.33663 14-15 ACATGGG 13635 0.0 22.137405 3 GCAGAGT 23030 0.0 21.521877 9 AGTACTT 12620 0.0 20.989153 12-13 GTGTAGC 910 0.0 20.835888 1 >>END_MODULE