FastQCFastQC Report
Fri 27 May 2016
SRR939458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867640
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104290.558405260114369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80120.4289905977597396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53030.2839412306440213No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22860.1224004626159217No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.11383350110299631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76150.045.6173021
GTATCAA126500.038.301271
TATCAAC157850.030.3131372
ATCAACG165900.028.9933
TCAACGC169300.028.3380854
CAACGCA171000.028.1744235
GTACATG111100.027.7272931
AACGCAG173850.027.6051586
CGACGCA2005.5479177E-1026.7117375
TACATGG112100.026.300972
GAGTACT103250.024.80104812-13
ACGCAGA191200.024.7695777
CGCAGAG193150.024.4943878
GTATCGA2501.8189894E-1024.2003461
ACATGGG118150.023.9617023
ATCGACG2703.45608E-1123.1821443
GCAGAGT188200.023.1533269
GTACTTT114850.023.13633214-15
TACCTGG11850.021.9047552
AGTACTT107250.021.63418412-13
Produced by FastQC (version 0.11.5)