##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939458_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1867640 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.75513749973228 31.0 26.0 31.0 10.0 33.0 2 26.787516330770384 31.0 25.0 31.0 10.0 34.0 3 27.072734574114925 31.0 25.0 31.0 16.0 34.0 4 30.915176907755242 35.0 30.0 37.0 19.0 37.0 5 31.380390225096914 35.0 30.0 37.0 19.0 37.0 6 31.210104195669402 35.0 30.0 37.0 17.0 37.0 7 31.200334111499004 35.0 30.0 37.0 17.0 37.0 8 31.12476494399349 35.0 30.0 37.0 17.0 37.0 9 32.14607740249727 35.0 30.0 39.0 17.0 39.0 10-11 31.218586826154933 35.0 29.0 38.0 13.5 39.0 12-13 30.940544483947654 35.0 27.0 38.0 13.0 39.0 14-15 31.715132198924845 36.0 27.0 39.0 16.5 40.0 16-17 31.656470733117732 36.0 28.0 39.0 16.0 40.0 18-19 31.697348257694202 36.0 27.5 38.5 15.5 40.0 20-21 31.651112366408945 36.0 27.0 39.0 13.0 40.0 22-23 31.81108243558716 36.0 28.5 39.0 13.5 40.0 24-25 31.842087340172625 36.0 29.5 39.0 13.5 41.0 26-27 31.927460056541946 36.0 30.0 39.0 15.0 41.0 28-29 31.49180061467949 36.0 29.0 39.0 10.0 40.0 30-31 31.15155249405667 35.5 27.0 39.0 10.0 40.0 32-33 30.98119926752479 35.0 27.5 39.0 9.0 40.0 34-35 30.488064348589663 35.0 26.5 38.5 9.0 40.0 36-37 30.08686256451993 35.0 25.5 38.0 9.0 40.0 38-39 29.693798055299737 35.0 25.0 38.0 8.0 40.0 40-41 29.497578227067315 34.5 24.5 38.0 7.5 40.0 42-43 29.18377096228395 34.0 24.0 38.0 4.0 40.0 44-45 28.765029930821786 34.0 24.0 38.0 2.0 40.0 46-47 28.07770448266261 33.0 23.0 38.0 2.0 40.0 48-49 27.678146752050715 33.0 22.0 38.0 2.0 40.0 50-51 26.997346919106466 33.0 19.5 37.5 2.0 39.5 52-53 27.095552408387057 33.0 20.0 38.0 2.0 39.5 54-55 27.218382022231264 33.0 20.0 38.0 2.0 40.0 56-57 26.779197007988692 33.0 18.5 38.0 2.0 40.0 58-59 26.3970532329571 33.0 17.5 38.0 2.0 40.0 60-61 25.787472692810177 31.5 13.0 37.0 2.0 39.5 62-63 25.23976863849564 31.0 10.5 36.5 2.0 39.0 64-65 24.72086188987171 31.0 8.0 36.0 2.0 39.0 66-67 24.34788048017819 31.0 7.5 36.0 2.0 39.0 68-69 23.658503780171767 30.0 4.5 35.0 2.0 38.0 70-71 23.050343749330708 29.0 2.0 35.0 2.0 38.0 72-73 22.57289788181877 29.0 2.0 34.0 2.0 37.5 74-75 21.84582655115547 28.5 2.0 34.0 2.0 36.5 76-77 21.08092673106166 27.5 2.0 34.0 2.0 36.0 78-79 20.578467745389904 26.5 2.0 33.5 2.0 35.5 80-81 20.0296240174766 26.0 2.0 33.0 2.0 35.0 82-83 19.29876020003855 25.5 2.0 33.0 2.0 35.0 84-85 18.629993735409393 24.5 2.0 32.0 2.0 35.0 86-87 17.867075025165448 22.5 2.0 32.0 2.0 34.0 88-89 17.086837131352937 20.0 2.0 31.5 2.0 34.0 90-91 16.283154676490117 15.5 2.0 31.0 2.0 34.0 92-93 15.44839235612859 4.5 2.0 31.0 2.0 34.0 94-95 14.404655340429633 2.0 2.0 30.5 2.0 34.0 96-97 13.322649172217343 2.0 2.0 29.5 2.0 34.0 98-99 12.311900848129191 2.0 2.0 29.0 2.0 34.0 100-101 11.012070848771712 2.0 2.0 26.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.8794512977755566 1101 2 3.8186812757280215 1101 3 3.24875617095811 1101 4 3.206923114005285 1101 5 2.568965519235821 1101 6 2.8879349619323627 1101 7 2.5736786710650286 1101 8 2.5253449549954574 1101 9 2.889531472582213 1101 10-11 3.538760105264547 1101 12-13 3.605482873800721 1101 14-15 3.075272513468253 1101 16-17 2.8502885164693623 1101 18-19 3.0817676452895704 1101 20-21 2.9404674534591386 1101 22-23 2.7785177141438417 1101 24-25 3.0433338025659005 1101 26-27 2.804677121914441 1101 28-29 3.259235056349109 1101 30-31 3.343163910495779 1101 32-33 3.1092424268412167 1101 34-35 3.393433024653593 1101 36-37 3.3016575526901875 1101 38-39 3.3574304628883205 1101 40-41 3.2171472521745734 1101 42-43 3.2510871950610998 1101 44-45 3.3174652658522525 1101 46-47 3.3779107411904903 1101 48-49 3.1565877213199407 1101 50-51 3.234937295927484 1101 52-53 3.003107088665452 1101 54-55 2.819908604028864 1101 56-57 2.9932471435704464 1101 58-59 3.108015632591215 1101 60-61 3.0083757330271332 1101 62-63 3.0422623415035446 1101 64-65 2.896091655209432 1101 66-67 2.4992023045465217 1101 68-69 2.7182481020084595 1101 70-71 2.2341945869899646 1101 72-73 2.2242587516193986 1101 74-75 2.4098425025838637 1101 76-77 2.2338505483318123 1101 78-79 1.6251452602792575 1101 80-81 1.8177321811302 1101 82-83 1.7803269668645214 1101 84-85 1.7423755733363073 1101 86-87 1.4266796541669144 1101 88-89 1.4825399996278605 1101 90-91 1.481698606327324 1101 92-93 1.3385401046629735 1101 94-95 1.4222607115759693 1101 96-97 1.3265496115596793 1101 98-99 1.3425524897903678 1101 100-101 0.8988173321636861 1102 1 3.5872826230767636 1102 2 2.501914207454927 1102 3 2.7502621950544963 1102 4 2.946882953362717 1102 5 1.8039052782719658 1102 6 2.5822120703660936 1102 7 2.4275943337156285 1102 8 2.3807666417424507 1102 9 2.663125850092257 1102 10-11 2.0395131173127403 1102 12-13 3.15317363685293 1102 14-15 3.1505737182875286 1102 16-17 3.0508407253047025 1102 18-19 3.0511451553297313 1102 20-21 2.929172272736249 1102 22-23 2.809391208119745 1102 24-25 2.9507133206381972 1102 26-27 2.6811831460108273 1102 28-29 3.044626622614164 1102 30-31 3.357471139411448 1102 32-33 2.7964914228251523 1102 34-35 3.1527201732479675 1102 36-37 2.9688262273889876 1102 38-39 3.09839431831001 1102 40-41 2.7394866096042954 1102 42-43 2.5497819741775665 1102 44-45 2.596330727699641 1102 46-47 2.7323284118732225 1102 48-49 2.627973263488613 1102 50-51 2.550951352152385 1102 52-53 2.5482878115570173 1102 54-55 2.530249969490715 1102 56-57 2.7562993524057866 1102 58-59 2.7515899297799677 1102 60-61 2.985785371581354 1102 62-63 2.8828747913027435 1102 64-65 2.818029406213448 1102 66-67 2.3779673647874837 1102 68-69 2.415537258634963 1102 70-71 2.2236524183152646 1102 72-73 2.0791784303342595 1102 74-75 2.3029147917404913 1102 76-77 2.3485593836731766 1102 78-79 2.0234886337732334 1102 80-81 2.1518185265117253 1102 82-83 2.0900659226878133 1102 84-85 2.1025663363885165 1102 86-87 1.8295410999500454 1102 88-89 1.777720722519426 1102 90-91 1.6428431846405758 1102 92-93 1.7140421126951022 1102 94-95 1.6044896272386193 1102 96-97 1.411137964973335 1102 98-99 1.1575625299510097 1102 100-101 0.5391285771436056 1103 1 3.8412906415518293 1103 2 3.6108738439611265 1103 3 3.1661133120040823 1103 4 2.8532829584340007 1103 5 2.546576058527524 1103 6 2.5834520960345806 1103 7 2.597287921081115 1103 8 2.3838191626566037 1103 9 2.7430065677174937 1103 10-11 3.4367355670924304 1103 12-13 2.6079340334660905 1103 14-15 3.199106850330029 1103 16-17 2.5278288136464084 1103 18-19 2.7135562128673136 1103 20-21 2.89197552557275 1103 22-23 2.9587465215541577 1103 24-25 2.8710153397881264 1103 26-27 2.8459781931249744 1103 28-29 2.359788191668425 1103 30-31 2.855458596768802 1103 32-33 2.833004278521866 1103 34-35 2.586947002858789 1103 36-37 2.904095351481274 1103 38-39 3.0887220693335316 1103 40-41 3.0818324377931994 1103 42-43 2.803565442192415 1103 44-45 2.8511707531730757 1103 46-47 2.6868360977892998 1103 48-49 2.3995799671366207 1103 50-51 2.7360849170091868 1103 52-53 2.810454252941785 1103 54-55 2.767891892208297 1103 56-57 2.802019386500401 1103 58-59 2.8593168549649235 1103 60-61 2.8266588216711703 1103 62-63 3.0843528745926783 1103 64-65 3.101063680269359 1103 66-67 3.039255304306611 1103 68-69 2.9277371022054552 1103 70-71 2.746896560498371 1103 72-73 2.6644626219818583 1103 74-75 2.468337166036118 1103 76-77 2.4699346369934005 1103 78-79 2.2631155484255316 1103 80-81 2.187390391436214 1103 82-83 2.422285280936741 1103 84-85 2.437667913166731 1103 86-87 2.342354861065413 1103 88-89 2.0932704396004738 1103 90-91 1.8026927577752758 1103 92-93 1.8854019083614997 1103 94-95 1.617693121156007 1103 96-97 1.5640676779973575 1103 98-99 1.608975637456311 1103 100-101 1.3191234961103913 1104 1 3.939941074535362 1104 2 3.6321699540105 1104 3 2.9537497631737217 1104 4 2.965068693413123 1104 5 2.0183191899808754 1104 6 2.6159912218897468 1104 7 2.7946625094252546 1104 8 2.6058123142481797 1104 9 2.6414703629643057 1104 10-11 3.5624452921809784 1104 12-13 3.4853074582685615 1104 14-15 3.4987364954875027 1104 16-17 3.4904030483115633 1104 18-19 3.3097175146019318 1104 20-21 2.9971748845413693 1104 22-23 2.697187300345103 1104 24-25 3.0278202449406386 1104 26-27 3.067439860550852 1104 28-29 2.80788528286136 1104 30-31 2.426766507109196 1104 32-33 2.5325873584590646 1104 34-35 3.176574806134507 1104 36-37 3.376471600345404 1104 38-39 3.4154856711120978 1104 40-41 3.2297098692773183 1104 42-43 3.1198147726848866 1104 44-45 3.150263967935075 1104 46-47 3.07689094912212 1104 48-49 2.70137087916957 1104 50-51 2.792976922912633 1104 52-53 2.944248472824782 1104 54-55 2.530813328649952 1104 56-57 2.9598195437433965 1104 58-59 3.0876835769194493 1104 60-61 3.073637264117547 1104 62-63 3.235474326202766 1104 64-65 3.411517324910541 1104 66-67 3.0841027696464955 1104 68-69 3.132583912758733 1104 70-71 3.198793769364464 1104 72-73 2.8380779956176987 1104 74-75 3.015028279639708 1104 76-77 2.8234881968891834 1104 78-79 2.580116941378048 1104 80-81 2.6728926386732716 1104 82-83 2.661148020234158 1104 84-85 2.3947235686151913 1104 86-87 2.7092539377220817 1104 88-89 2.6335893960496293 1104 90-91 2.4995510539451473 1104 92-93 2.4666992007953237 1104 94-95 2.8711559530656903 1104 96-97 2.7270337011673824 1104 98-99 2.2541552539021 1104 100-101 1.8884935442866695 1105 1 3.6479817325246344 1105 2 3.517883268528795 1105 3 3.022978841766161 1105 4 3.0126768538807767 1105 5 0.2930353463117683 1105 6 2.0245300701617275 1105 7 2.2198953187407007 1105 8 2.2388354990246704 1105 9 2.8376099121204916 1105 10-11 2.8048141196899827 1105 12-13 3.478866350096691 1105 14-15 3.208319825134499 1105 16-17 3.1635103187047093 1105 18-19 3.1594404343816116 1105 20-21 3.1669964691859924 1105 22-23 2.8552031163383127 1105 24-25 2.58472331645439 1105 26-27 2.90537889684904 1105 28-29 2.6736670281151866 1105 30-31 2.8618413567185037 1105 32-33 2.8523621693735173 1105 34-35 3.094628348435517 1105 36-37 3.1781321259713167 1105 38-39 3.284013655958251 1105 40-41 3.266127257650151 1105 42-43 3.21035802779193 1105 44-45 2.614416874495177 1105 46-47 2.766214315545522 1105 48-49 3.0716120770240813 1105 50-51 3.0099641917970885 1105 52-53 3.06324803651831 1105 54-55 3.07185349352444 1105 56-57 3.2725289370911455 1105 58-59 3.256646401466039 1105 60-61 3.3953848834068587 1105 62-63 3.161837015620076 1105 64-65 3.1011804935199443 1105 66-67 2.863212955889292 1105 68-69 3.061511939885172 1105 70-71 3.0575045093486253 1105 72-73 3.051865024044673 1105 74-75 2.856583258213199 1105 76-77 3.0677356566961365 1105 78-79 2.9209431903577077 1105 80-81 2.9235138049851948 1105 82-83 3.0897263502839394 1105 84-85 2.8508172613557896 1105 86-87 3.000236362999363 1105 88-89 2.9783407735348177 1105 90-91 2.808873484428304 1105 92-93 3.0537073024989656 1105 94-95 2.6758414049688373 1105 96-97 2.8448106056078846 1105 98-99 2.6497259748973825 1105 100-101 2.643813037500637 1106 1 3.1307172310739695 1106 2 2.5417137217175103 1106 3 1.9110535819459358 1106 4 1.4024304397509582 1106 5 1.7409598205517618 1106 6 2.145812321171693 1106 7 2.078262222662719 1106 8 0.46315945409859083 1106 9 1.787286524410078 1106 10-11 0.6716711962688784 1106 12-13 1.4453780275382009 1106 14-15 1.546558844563382 1106 16-17 1.9233356456977333 1106 18-19 1.9627391598061372 1106 20-21 1.7539258324295623 1106 22-23 2.430292798671676 1106 24-25 2.8067679740343827 1106 26-27 2.5226269846063154 1106 28-29 2.268439859752444 1106 30-31 2.251079168365205 1106 32-33 2.2279464921141674 1106 34-35 2.580451850946911 1106 36-37 1.787594383015442 1106 38-39 2.7468200369950218 1106 40-41 2.3118531508681635 1106 42-43 2.376158627034055 1106 44-45 2.5785987325252684 1106 46-47 2.7070504224451533 1106 48-49 2.596508471514394 1106 50-51 2.3584669878175752 1106 52-53 2.430854640136154 1106 54-55 2.5687746214582674 1106 56-57 2.504598874605257 1106 58-59 2.497290121852604 1106 60-61 2.572140674214811 1106 62-63 2.6849070309332 1106 64-65 2.718662571284799 1106 66-67 2.545984294165077 1106 68-69 2.674230132898817 1106 70-71 2.416952297674598 1106 72-73 2.455499921833855 1106 74-75 2.404898814789206 1106 76-77 2.7942165181398977 1106 78-79 2.570429361109454 1106 80-81 2.5066925681327525 1106 82-83 2.6500975511780567 1106 84-85 2.404448767297925 1106 86-87 2.446092284927108 1106 88-89 2.764166505651957 1106 90-91 2.5455677191422197 1106 92-93 2.4253021726979593 1106 94-95 2.53000989010828 1106 96-97 2.437542992196853 1106 98-99 2.20134834514856 1106 100-101 1.9786366092721206 1107 1 3.2084867068478715 1107 2 3.4472628372979344 1107 3 -0.1055073457278013 1107 4 0.7152758929081564 1107 5 1.5469998341170204 1107 6 1.9692499358269338 1107 7 2.2988931264782515 1107 8 1.972631340880195 1107 9 2.3506776125862814 1107 10-11 1.896245868689551 1107 12-13 2.430478919196556 1107 14-15 2.6203817008449555 1107 16-17 1.2668064446799434 1107 18-19 0.3795063925515727 1107 20-21 -1.3966665819297823 1107 22-23 0.6182410077195506 1107 24-25 1.3743782513091922 1107 26-27 1.4393614941248067 1107 28-29 2.2935681992569066 1107 30-31 2.940349693265592 1107 32-33 1.551575859921254 1107 34-35 1.0860058750113701 1107 36-37 1.21198488812324 1107 38-39 2.2679473422472007 1107 40-41 2.8568892356629654 1107 42-43 2.03545975806777 1107 44-45 1.9953932346264942 1107 46-47 2.5408510120582015 1107 48-49 1.989924706994703 1107 50-51 2.543737222175377 1107 52-53 2.252280148189044 1107 54-55 2.45872578947915 1107 56-57 2.779467287568412 1107 58-59 2.525333927345745 1107 60-61 2.754441027823983 1107 62-63 2.4890324878188608 1107 64-65 1.9560967281857309 1107 66-67 2.70886874250105 1107 68-69 2.733471816736916 1107 70-71 1.538986383377722 1107 72-73 2.4781165057669448 1107 74-75 2.8493729525321783 1107 76-77 3.02382139509065 1107 78-79 2.8173297030175988 1107 80-81 2.541718568223981 1107 82-83 2.678798424194092 1107 84-85 2.9347212228687063 1107 86-87 3.1316124057452264 1107 88-89 3.008960785631885 1107 90-91 2.755136436927195 1107 92-93 2.800619984644518 1107 94-95 2.8075637148585404 1107 96-97 2.539837534954213 1107 98-99 2.5314903788277014 1107 100-101 1.9237883905064397 1108 1 2.324887191548278 1108 2 2.1672077928637563 1108 3 1.5968243329340766 1108 4 1.4251577124782315 1108 5 1.7419479628442467 1108 6 -0.42335763930261905 1108 7 0.12075234123979683 1108 8 -0.8747852099330302 1108 9 1.121772690417984 1108 10-11 2.510604002592988 1108 12-13 1.6296665650876 1108 14-15 1.7990292002946084 1108 16-17 -0.09025131082400151 1108 18-19 0.12133599775080128 1108 20-21 0.8786787968564447 1108 22-23 -0.9765747902611288 1108 24-25 0.9500486064454527 1108 26-27 1.1693661150410968 1108 28-29 1.727184919040976 1108 30-31 0.2515732395114476 1108 32-33 0.5098140810469758 1108 34-35 1.0379617323698334 1108 36-37 0.012890825703188113 1108 38-39 1.5687073887736815 1108 40-41 0.04110216272587408 1108 42-43 0.9964649511447234 1108 44-45 1.9071560447782367 1108 46-47 1.692475323630923 1108 48-49 1.591567760051941 1108 50-51 1.720127066868958 1108 52-53 1.0210226243258766 1108 54-55 1.2209485345017477 1108 56-57 2.0594407718384566 1108 58-59 1.8730312285719712 1108 60-61 1.215421306625874 1108 62-63 1.3610433945695632 1108 64-65 0.9412416226681977 1108 66-67 1.2984546498962999 1108 68-69 1.9531332949541564 1108 70-71 2.3030688984651135 1108 72-73 2.111873439620414 1108 74-75 2.1956596843544247 1108 76-77 2.2593845023296204 1108 78-79 2.2142040646667667 1108 80-81 2.3461194456031116 1108 82-83 2.494465140110865 1108 84-85 2.509191850301878 1108 86-87 2.4762306248480535 1108 88-89 2.3666862684978085 1108 90-91 2.184648746810204 1108 92-93 2.087357508666342 1108 94-95 2.388211471135948 1108 96-97 2.4926319250031774 1108 98-99 2.1195795704450013 1108 100-101 1.8684587436594722 1109 1 1.656651583968479 1109 2 1.3247644019480553 1109 3 0.7504777048381825 1109 4 2.1837124304194475 1109 5 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50-51 -0.4098285599488527 2312 52-53 -0.7792620103722108 2312 54-55 -0.4293904790960852 2312 56-57 0.27527715039017764 2312 58-59 -0.15178015433005854 2312 60-61 0.30455857761302596 2312 62-63 -0.5168486306871038 2312 64-65 -2.1226029344700414 2312 66-67 1.074790506410583 2312 68-69 0.3622562099117701 2312 70-71 -2.400346407175377 2312 72-73 -3.015853584517604 2312 74-75 -0.9572286120634033 2312 76-77 -0.4784656977847277 2312 78-79 -0.5481157081226513 2312 80-81 -1.5761404793229339 2312 82-83 0.25406721085936823 2312 84-85 1.5545506888928742 2312 86-87 1.6541944302523852 2312 88-89 1.8473426062808898 2312 90-91 2.108074866083328 2312 92-93 2.0914138638506703 2312 94-95 1.548756316637725 2312 96-97 1.0546639557288096 2312 98-99 0.5677779198123414 2312 100-101 1.3931806344293545 2313 1 -8.187260521036695 2313 2 -4.687083719737714 2313 3 -2.0033661703997296 2313 4 0.24015947500907586 2313 5 0.4150109914472502 2313 6 -3.0582796879384695 2313 7 -0.9897877182112431 2313 8 -2.074001196212791 2313 9 0.7463248700811249 2313 10-11 -1.5730748055361516 2313 12-13 -2.5385624726705522 2313 14-15 -3.6267976266748647 2313 16-17 -3.6774881499686884 2313 18-19 -0.9088432803865665 2313 20-21 -2.838000307527885 2313 22-23 -1.5280372533542312 2313 24-25 -2.8289761202372645 2313 26-27 -2.0688630539693555 2313 28-29 -2.673128648946463 2313 30-31 -4.709087790806581 2313 32-33 -5.958112857674454 2313 34-35 -4.668142499454223 2313 36-37 -2.1484107470743368 2313 38-39 -1.8318913404540815 2313 40-41 -3.877965170952468 2313 42-43 -3.4124845492215314 2313 44-45 -3.212510424384977 2313 46-47 -1.8906683188828204 2313 48-49 -2.9082394240737806 2313 50-51 -3.6500228482545545 2313 52-53 -0.7280023586113273 2313 54-55 -1.9788576183559279 2313 56-57 -2.50738727265232 2313 58-59 -1.8790264636876408 2313 60-61 -2.077482881697417 2313 62-63 -3.975892345555888 2313 64-65 -5.353140091511797 2313 66-67 -4.1412794517004805 2313 68-69 -2.8551831943344084 2313 70-71 -1.7768557814667858 2313 72-73 -2.0896091488248807 2313 74-75 -2.995281313363037 2313 76-77 -3.135086775917749 2313 78-79 -3.2121079270430855 2313 80-81 -4.066772154999899 2313 82-83 -1.8437309345704413 2313 84-85 -2.0607524987242307 2313 86-87 -3.037033357271632 2313 88-89 -3.72705740326645 2313 90-91 -3.102878316163398 2313 92-93 -1.4139947024973551 2313 94-95 -0.42944830666016465 2313 96-97 -0.6007392402422163 2313 98-99 -0.4685610345155844 2313 100-101 -0.09730425094293338 2314 1 -7.644594459982084 2314 2 -3.5118266612446583 2314 3 -24.79843258406197 2314 4 -13.25291441350451 2314 5 -6.827113938327116 2314 6 -5.4364982508702475 2314 7 -4.688127972276263 2314 8 -3.8433375514580597 2314 9 -3.746697526036808 2314 10-11 -14.413134044847443 2314 12-13 -13.117556610991425 2314 14-15 -4.957110969430538 2314 16-17 -3.5568195634810635 2314 18-19 -3.5498179315255847 2314 20-21 -4.620763497074467 2314 22-23 -4.614683584367331 2314 24-25 -5.470924323498501 2314 26-27 -4.542151565862181 2314 28-29 -3.8126847614396695 2314 30-31 -4.282399704840412 2314 32-33 -4.587540146953351 2314 34-35 -3.6849393957692875 2314 36-37 -4.1583687615583 2314 38-39 -1.12914829442526 2314 40-41 -2.375500068578667 2314 42-43 -4.438501658530665 2314 44-45 -5.365531606204627 2314 46-47 -4.511534036706031 2314 48-49 -2.8913174473498806 2314 50-51 -2.570734357880948 2314 52-53 -0.7941347340323901 2314 54-55 -2.9296522151896838 2314 56-57 -2.8695198417462784 2314 58-59 -0.9752255947726773 2314 60-61 -3.802203766013985 2314 62-63 -4.871081495570149 2314 64-65 -4.21276509621913 2314 66-67 -5.238259999103072 2314 68-69 -4.406562806266194 2314 70-71 -5.288112441854452 2314 72-73 -4.48669045224344 2314 74-75 -3.7839780408291546 2314 76-77 -3.5902785927769756 2314 78-79 -4.507092682988345 2314 80-81 -6.852124950921702 2314 82-83 -6.178148490249477 2314 84-85 -4.774834823536725 2314 86-87 -4.001398698124394 2314 88-89 -3.2119878078352855 2314 90-91 -5.364116915785427 2314 92-93 -5.302176467579825 2314 94-95 -5.650627743825224 2314 96-97 -4.842137896674959 2314 98-99 -5.712679373263306 2314 100-101 -4.845406525600297 2315 1 -24.352978421099944 2315 2 -24.41826651543664 2315 3 -13.126772159592557 2315 4 -28.645988532581057 2315 5 -15.467696108548724 2315 6 -12.290398220986578 2315 7 -9.103874237197235 2315 8 -8.428643282599115 2315 9 -5.915749576708581 2315 10-11 -28.992113388711356 2315 12-13 -28.677398652303697 2315 14-15 -23.46968258783584 2315 16-17 -14.099705278936984 2315 18-19 -9.440951508479063 2315 20-21 -7.466728747073329 2315 22-23 -4.053707253141024 2315 24-25 -6.0599237971212325 2315 26-27 -5.7618784261203935 2315 28-29 -17.49677117832743 2315 30-31 -16.50957851989996 2315 32-33 -10.024345294831992 2315 34-35 -7.513308172344136 2315 36-37 -5.269895513507187 2315 38-39 -5.618820984850199 2315 40-41 -8.829514112384786 2315 42-43 -7.995018952375084 2315 44-45 -5.5046772367423955 2315 46-47 -4.407098208465815 2315 48-49 -4.834884722763508 2315 50-51 -3.317446396144174 2315 52-53 -7.161839213396732 2315 54-55 -6.805206086944768 2315 56-57 -7.6817411993141995 2315 58-59 -7.558333283673097 2315 60-61 -5.312892485916056 2315 62-63 -4.115997100759895 2315 64-65 -5.103373433610269 2315 66-67 -7.191454517498212 2315 68-69 -6.90530791983171 2315 70-71 -7.935293289610069 2315 72-73 -7.40777406128014 2315 74-75 -7.80259996391133 2315 76-77 -7.461929763928467 2315 78-79 -7.389961985181987 2315 80-81 -8.166287229847043 2315 82-83 -7.372862570310396 2315 84-85 -6.591895969764938 2315 86-87 -6.756890769851259 2315 88-89 -6.32462875027813 2315 90-91 -5.884732473798422 2315 92-93 -5.850179191999329 2315 94-95 -5.874222035893309 2315 96-97 -5.810032296597292 2315 98-99 -5.689752761176045 2315 100-101 -5.2371093332996645 2316 1 -1.3778942460157708 2316 2 1.5999153027451811 2316 3 2.013688628059242 2316 4 2.025391938799416 2316 5 -1.4214395353258595 2316 6 -2.88162509648312 2316 7 -2.8388948553639217 2316 8 -4.217399761243232 2316 9 -3.6765145296083652 2316 10-11 -3.3163558129537805 2316 12-13 -3.5649407398457846 2316 14-15 -2.40056822321586 2316 16-17 -2.7473256776809762 2316 18-19 -4.4208686574973335 2316 20-21 -1.9185068275030979 2316 22-23 -2.5209716574840826 2316 24-25 -2.955786922759639 2316 26-27 -2.6548909483463987 2316 28-29 -1.9919155054942301 2316 30-31 0.7421730784808496 2316 32-33 -1.6629863874056632 2316 34-35 -7.477899781317767 2316 36-37 -3.208892222166817 2316 38-39 -1.187816180249886 2316 40-41 -1.1568776352539238 2316 42-43 -1.3937213319763408 2316 44-45 -0.8353926303791361 2316 46-47 0.17712983980717922 2316 48-49 0.6419813159002814 2316 50-51 1.109890644676014 2316 52-53 0.9457554599282787 2316 54-55 -0.9810771571978592 2316 56-57 0.4885799901081107 2316 58-59 -3.0665219115246423 2316 60-61 -3.502346955707605 2316 62-63 -4.619583526818229 2316 64-65 -5.126592515818114 2316 66-67 -2.7217821382752696 2316 68-69 -2.2671510704389064 2316 70-71 -2.960873057324669 2316 72-73 -3.097506592585706 2316 74-75 -2.6982266426840766 2316 76-77 -2.686575093629976 2316 78-79 -3.058331069281408 2316 80-81 -3.028240091799905 2316 82-83 -3.875822885068464 2316 84-85 -3.2629132587597525 2316 86-87 -2.617430625334933 2316 88-89 -3.905614639129187 2316 90-91 -4.4715198229453215 2316 92-93 -4.581376937242018 2316 94-95 -5.643937921339532 2316 96-97 -5.567050039570092 2316 98-99 -5.332517487086839 2316 100-101 -5.063156320020809 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26821.0 3 9887.0 4 9028.0 5 9429.0 6 11901.0 7 14548.0 8 18112.0 9 21975.0 10 25824.0 11 29464.0 12 33501.0 13 37165.0 14 40215.0 15 42835.0 16 44575.0 17 44424.0 18 41170.0 19 41396.0 20 43799.0 21 46528.0 22 50392.0 23 54786.0 24 59117.0 25 63693.0 26 68984.0 27 73510.0 28 78560.0 29 84456.0 30 89051.0 31 94808.0 32 98964.0 33 103147.0 34 105014.0 35 101078.0 36 87053.0 37 52516.0 38 9823.0 39 91.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.283698612560855 19.189107568975555 14.693083086666903 20.83411073179669 2 15.902884207332225 20.822714809350312 40.35735410203018 22.917046881287284 3 19.64169204884198 26.604703501928682 30.053992350182046 23.69961209904729 4 12.561180371682584 17.555725898960894 37.250643022329285 32.632450707027246 5 13.032618151899086 38.265371251787414 33.528621739069806 15.173388857243692 6 30.175285711529433 36.314305192007154 18.047578928412943 15.462830168050473 7 27.525143982326593 34.68754790031803 20.451385799746387 17.335922317608986 8 26.92600426005868 33.888584564951955 19.692319566576014 19.493091608413348 9 27.87797093448602 16.703425099890932 20.60022993002945 34.8183740355936 10-11 25.036990368185418 27.45567633393745 26.35236104235789 21.154972255519237 12-13 26.83913128837421 25.253723610368517 27.29385830688724 20.61328679437003 14-15 23.661145177431067 25.815613374094486 27.09515942445594 23.428082024018504 16-17 21.401415376205495 29.444969948042132 27.474563690589438 21.679050985162934 18-19 20.985970674335917 26.751521862401557 31.814910017440184 20.44759744582234 20-21 22.463831048766902 25.554629899166 31.905097346707816 20.076441705359283 22-23 22.05869224572301 25.916273807784435 31.605451277232593 20.41958266925996 24-25 21.42246409976537 25.98919862691774 32.18868700550793 20.39965026780895 26-27 21.187573092908494 26.33730865811474 31.927786753433523 20.54733149554324 28-29 20.842886642797193 26.321359014424274 32.28504750782822 20.550706834950315 30-31 20.94684204950645 26.32201587456971 32.219690742248105 20.51145133367573 32-33 20.80931179430305 26.458158814059075 31.928205139206046 20.80432425243183 34-35 21.43566659204457 26.396329129026075 31.65721378962735 20.510790489302007 36-37 21.417589979618352 26.18790842708542 31.604820716309096 20.78968087698713 38-39 21.170516151271865 26.54154609298241 31.801740563255006 20.48619719249072 40-41 21.286254634232378 26.35605325705016 31.814817803064926 20.542874305652536 42-43 21.094197939593467 26.50275838646789 31.443729371743 20.95931430219564 44-45 21.23357183679115 26.943447552650007 30.956144028496034 20.86683658206281 46-47 21.35778083040849 27.045807690699164 30.271578685488603 21.324832793403743 48-49 21.62562104681748 27.215350749009943 29.911062610099233 21.247965594073342 50-51 21.60772892347635 27.560461991316544 29.553349935816662 21.27845914939044 52-53 21.57288670555331 27.838690885527583 29.39936874470959 21.18905366420952 54-55 21.193860602187094 27.73730732579699 29.475723405790355 21.59310866622556 56-57 21.339065492528558 28.25111855128739 29.158936840696903 21.25087911548715 58-59 21.48076917405929 27.640386544038186 29.33697890961382 21.541865372288704 60-61 21.493543826262922 27.72781548811622 29.553904192659996 21.22473649296086 62-63 21.52354883230847 27.62679331188449 29.542688214151784 21.306969641655254 64-65 21.250272385096373 27.55371246479766 29.520165342108978 21.675849807996986 66-67 21.11093183470167 28.00366009764549 29.111659780308376 21.773748287344468 68-69 21.248015368897494 27.84398575032188 29.22146178198164 21.686537098798983 70-71 21.085486592585344 27.92050040409212 29.14079159166726 21.853221411655277 72-73 21.312713427923892 27.831108508914575 28.94496836883562 21.911209694325905 74-75 21.086639046112275 28.037731468262233 28.801412181946045 22.074217303679443 76-77 21.28945044578055 27.909372748269877 28.855907239244456 21.94526956670511 78-79 21.14049145299145 28.116552749459377 28.64087224968249 22.10208354786668 80-81 20.97051871803164 28.33314293774493 28.607044422240563 22.08929392198286 82-83 21.19328432329464 28.22033280204564 28.339684831271466 22.246698043388257 84-85 21.048990853778697 27.99848890149627 28.37183962300827 22.58068062171676 86-87 21.091404657462775 27.90434551566988 28.72160135170823 22.282648475159117 88-89 21.271815528087526 27.803953551719186 28.3835114608602 22.54071945933309 90-91 20.891755881847455 28.06676653297237 28.554104094938594 22.487373490241584 92-93 21.008427693021247 27.928841155345964 28.486782047552722 22.575949104080063 94-95 20.794814259445292 27.746153010462955 28.389461669592787 23.069571060498966 96-97 21.078538143526828 27.595888595729797 28.144685636050916 23.18088762469246 98-99 20.81783628625692 27.596722902737437 27.98874688348716 23.59669392751849 100-101 20.81791190248217 27.646296065710324 28.28634806205212 23.24944396975539 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3306.0 1 2319.5 2 1295.0 3 1256.5 4 1204.0 5 1232.5 6 1716.0 7 2904.0 8 4339.5 9 5154.0 10 4946.5 11 4338.5 12 4042.5 13 4079.5 14 4266.0 15 4479.0 16 4698.0 17 5000.0 18 5462.0 19 5992.0 20 6532.0 21 7353.5 22 8365.5 23 9491.5 24 10745.0 25 12397.5 26 14775.0 27 17363.5 28 19718.5 29 22133.5 30 24974.5 31 28461.5 32 32280.5 33 36290.0 34 40548.0 35 44299.0 36 48197.0 37 52127.0 38 54025.0 39 54309.0 40 54577.0 41 56158.5 42 58372.5 43 60169.5 44 61965.5 45 63217.0 46 63795.5 47 65060.0 48 68315.5 49 69085.0 50 66579.0 51 63486.5 52 60416.0 53 58177.0 54 55223.5 55 51081.5 56 46260.5 57 41221.0 58 36450.0 59 31774.5 60 27072.0 61 22254.5 62 17695.0 63 13791.5 64 10430.5 65 7860.0 66 5864.5 67 4306.0 68 3132.5 69 2236.0 70 1655.5 71 1275.0 72 1013.0 73 788.0 74 590.5 75 469.0 76 328.5 77 210.0 78 146.5 79 113.5 80 82.5 81 49.0 82 27.0 83 28.0 84 25.5 85 12.5 86 7.0 87 7.5 88 6.0 89 2.5 90 1.5 91 2.5 92 2.5 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.435597866826583 2 2.5964318605298664 3 1.029641686834722 4 0.952110685142747 5 0.17230301342871218 6 0.09487909875564884 7 0.09562870788802981 8 0.10376732132530894 9 0.09932321004047891 10-11 1.8414951489580433 12-13 1.4090242230836778 14-15 0.534846115953824 16-17 0.09129168362211132 18-19 0.09819879634190742 20-21 0.12298944121993532 22-23 0.13774067807500373 24-25 0.1490919020796299 26-27 0.14558480221027606 28-29 0.5748163457625666 30-31 0.17870146280867832 32-33 0.1603092673106166 34-35 0.14389818166241888 36-37 0.13286821871452742 38-39 0.43514810134715465 40-41 0.1236587350881326 42-43 0.1246760617677925 44-45 0.12839733567496947 46-47 0.1382493414148337 48-49 0.15233128440170482 50-51 0.16381636717997045 52-53 0.18301171531986893 54-55 0.18127155126255595 56-57 0.152036795099698 58-59 0.13645563384806494 60-61 0.11659099183996915 62-63 0.12162408172881284 64-65 0.1164303613116018 66-67 0.11329806600843845 68-69 0.10997836842217985 70-71 0.1256933884474524 72-73 0.13648240560279284 74-75 0.14587929151228288 76-77 0.16606519457711336 78-79 0.16748409757769162 80-81 0.16603842282238548 82-83 0.1599880062538819 84-85 0.14839583645670473 86-87 0.1467359876635754 88-89 0.14371077937932364 90-91 0.15064466385384762 92-93 0.14229187637874535 94-95 0.19717397357092373 96-97 0.19564798355143387 98-99 0.21302285236983573 100-101 0.23730483390803367 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1867640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.20357878657775 #Duplication Level Percentage of deduplicated Percentage of total 1 85.00857124066547 57.97888786147135 2 9.192994155593363 12.539902023511216 3 2.6863028474151696 5.49646403694866 4 1.0633536441864058 2.900980961970484 5 0.5683263985984242 1.9380947151649806 6 0.3246023435739521 1.3283424908552293 7 0.20809218755624376 0.993484233620451 8 0.14873474703260142 0.8115393630031794 9 0.10199173231246222 0.6260581035317309 >10 0.6225717195088428 7.898488632031192 >50 0.049155749367627594 2.325721657568651 >100 0.023795373902375506 2.9190635058749472 >500 8.377001594099091E-4 0.4106802617314037 >1k 4.1885007970495457E-4 0.47569892309066053 >5k 1.6754003188198185E-4 0.7607411915628326 >10k+ 8.377001594099092E-5 0.5958520380629952 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10429 0.558405260114369 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8012 0.4289905977597396 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5303 0.2839412306440213 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2286 0.1224004626159217 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2126 0.11383350110299631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.354350945578377E-5 2 5.354350945578377E-5 0.0 0.0 0.0 5.354350945578377E-5 3 5.354350945578377E-5 0.0 0.0 5.354350945578377E-5 5.354350945578377E-5 4 5.354350945578377E-5 0.0 0.0 5.354350945578377E-5 5.354350945578377E-5 5 5.354350945578377E-5 0.0 0.0 5.354350945578377E-5 5.354350945578377E-5 6 5.354350945578377E-5 0.0 0.0 5.354350945578377E-5 5.354350945578377E-5 7 5.354350945578377E-5 0.0 0.0 1.0708701891156754E-4 5.354350945578377E-5 8 5.354350945578377E-5 0.0 0.0 1.0708701891156754E-4 5.354350945578377E-5 9 5.354350945578377E-5 0.0 0.0 1.0708701891156754E-4 5.354350945578377E-5 10-11 5.354350945578377E-5 5.354350945578377E-5 0.0 1.0708701891156754E-4 5.354350945578377E-5 12-13 5.354350945578377E-5 5.354350945578377E-5 0.0 1.0708701891156754E-4 5.354350945578377E-5 14-15 5.354350945578377E-5 5.354350945578377E-5 0.0 1.0708701891156754E-4 5.354350945578377E-5 16-17 1.6063052836735132E-4 5.354350945578377E-5 0.0 1.0708701891156754E-4 5.354350945578377E-5 18-19 1.6063052836735132E-4 5.354350945578377E-5 0.0 1.0708701891156754E-4 5.354350945578377E-5 20-21 2.1417403782313509E-4 5.354350945578377E-5 0.0 1.6063052836735132E-4 5.354350945578377E-5 22-23 2.1417403782313509E-4 5.354350945578377E-5 0.0 4.015763209183783E-4 8.031526418367566E-5 24-25 3.2126105673470264E-4 8.031526418367566E-5 0.0 7.763808871088646E-4 1.0708701891156754E-4 26-27 3.2126105673470264E-4 1.0708701891156754E-4 0.0 0.0012315007174830267 1.0708701891156754E-4 28-29 3.480328114625945E-4 1.0708701891156754E-4 0.0 0.0024362296802381616 1.0708701891156754E-4 30-31 3.748045661904864E-4 1.0708701891156754E-4 0.0 0.006023644813775674 1.0708701891156754E-4 32-33 3.748045661904864E-4 1.0708701891156754E-4 0.0 0.013439420873401726 1.0708701891156754E-4 34-35 4.2834807564627017E-4 1.0708701891156754E-4 0.0 0.025218992953674156 1.0708701891156754E-4 36-37 4.818915851020539E-4 1.0708701891156754E-4 0.0 0.04449465635775631 1.0708701891156754E-4 38-39 5.354350945578377E-4 1.0708701891156754E-4 0.0 0.07340815146387955 1.0708701891156754E-4 40-41 5.354350945578377E-4 1.0708701891156754E-4 0.0 0.10644449679809814 1.0708701891156754E-4 42-43 5.354350945578377E-4 1.0708701891156754E-4 0.0 0.1395611573965004 1.0708701891156754E-4 44-45 6.157503587415134E-4 1.0708701891156754E-4 0.0 0.18052194213017497 1.0708701891156754E-4 46-47 6.425221134694053E-4 1.0708701891156754E-4 0.0 0.23240560279282946 1.0708701891156754E-4 48-49 6.425221134694053E-4 1.0708701891156754E-4 0.0 0.29232078987385146 1.0708701891156754E-4 50-51 6.692938681972971E-4 1.0708701891156754E-4 0.0 0.35510055471075797 1.0708701891156754E-4 52-53 6.96065622925189E-4 1.0708701891156754E-4 0.0 0.41753228673620185 1.0708701891156754E-4 54-55 6.96065622925189E-4 1.0708701891156754E-4 0.0 0.48879869782185004 1.0708701891156754E-4 56-57 6.96065622925189E-4 1.0708701891156754E-4 0.0 0.5743076824227367 1.0708701891156754E-4 58-59 6.96065622925189E-4 1.0708701891156754E-4 0.0 0.6748356214259708 1.0708701891156754E-4 60-61 6.96065622925189E-4 1.3385877363945943E-4 0.0 0.7860990340750894 1.0708701891156754E-4 62-63 6.96065622925189E-4 1.6063052836735132E-4 0.0 0.9000396221969973 1.0708701891156754E-4 64-65 6.96065622925189E-4 1.6063052836735132E-4 0.0 1.0114101218650275 1.0708701891156754E-4 66-67 7.496091323809728E-4 1.6063052836735132E-4 0.0 1.140048403332548 1.0708701891156754E-4 68-69 7.496091323809728E-4 2.1417403782313509E-4 0.0 1.2912285022809535 1.0708701891156754E-4 70-71 7.496091323809728E-4 2.1417403782313509E-4 0.0 1.4674134201452098 1.0708701891156754E-4 72-73 7.496091323809728E-4 2.1417403782313509E-4 0.0 1.6408676190272216 1.0708701891156754E-4 74-75 7.496091323809728E-4 2.1417403782313509E-4 0.0 1.8172399391745733 1.0708701891156754E-4 76-77 7.496091323809728E-4 2.1417403782313509E-4 0.0 2.009996573215395 1.0708701891156754E-4 78-79 7.496091323809728E-4 2.1417403782313509E-4 0.0 2.2340761602878496 1.0708701891156754E-4 80-81 8.031526418367565E-4 2.1417403782313509E-4 0.0 2.482170011351224 1.0708701891156754E-4 82-83 8.031526418367565E-4 2.1417403782313509E-4 0.0 2.7344134843974213 1.0708701891156754E-4 84-85 8.031526418367565E-4 2.1417403782313509E-4 0.0 2.9856931742734147 1.0708701891156754E-4 86-87 8.031526418367565E-4 2.6771754727891885E-4 0.0 3.2479225118331154 1.0708701891156754E-4 88-89 8.031526418367565E-4 2.6771754727891885E-4 0.0 3.533122014949348 1.0708701891156754E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7615 0.0 45.617302 1 GTATCAA 12650 0.0 38.30127 1 TATCAAC 15785 0.0 30.313137 2 ATCAACG 16590 0.0 28.993 3 TCAACGC 16930 0.0 28.338085 4 CAACGCA 17100 0.0 28.174423 5 GTACATG 11110 0.0 27.727293 1 AACGCAG 17385 0.0 27.605158 6 CGACGCA 200 5.5479177E-10 26.711737 5 TACATGG 11210 0.0 26.30097 2 GAGTACT 10325 0.0 24.801048 12-13 ACGCAGA 19120 0.0 24.769577 7 CGCAGAG 19315 0.0 24.494387 8 GTATCGA 250 1.8189894E-10 24.200346 1 ACATGGG 11815 0.0 23.961702 3 ATCGACG 270 3.45608E-11 23.182144 3 GCAGAGT 18820 0.0 23.153326 9 GTACTTT 11485 0.0 23.136332 14-15 TACCTGG 1185 0.0 21.904755 2 AGTACTT 10725 0.0 21.634184 12-13 >>END_MODULE