FastQCFastQC Report
Fri 27 May 2016
SRR939458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867640
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148330.7942108757576406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135120.7234798997665502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90710.4856931742734146No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37330.1998779207984408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35670.1909896982287807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26950.14429975798333727No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19420.10398149536313209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88700.044.9957581
ACCGTAC550.002606233634.4743588
GTATCAA139500.033.2708441
TATCAAC180050.025.693852
ATCAACG180750.025.4845623
TCAACGC183450.025.1380374
CAACGCA186750.024.7989255
GTACATG128700.024.410611
AACGCAG191000.024.2719356
TACATGG127550.024.0308862
ACATGGG129200.022.8750363
TGGTATC47750.021.7614822
TACCTGG12250.021.6905042
GAGTACT122900.021.59675612-13
ATACCGT1353.8503605E-421.0676636
GTGGTAT50750.021.0399971
ACGCAGA220000.021.029367
CGCAGAG219800.021.0269288
AGTACTT125500.020.4506512-13
GTACTTT130150.020.37550214-15
Produced by FastQC (version 0.11.5)