##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939458_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1867640 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89251086933242 31.0 31.0 31.0 28.0 34.0 2 31.13862361054593 31.0 31.0 34.0 28.0 34.0 3 31.129282409886272 31.0 31.0 34.0 28.0 34.0 4 35.054060739757126 35.0 35.0 37.0 32.0 37.0 5 34.637497590542075 35.0 35.0 37.0 32.0 37.0 6 34.706412370692426 35.0 35.0 37.0 31.0 37.0 7 34.54575239339487 35.0 35.0 37.0 30.0 37.0 8 34.45039836371035 35.0 35.0 37.0 30.0 37.0 9 35.923662483133796 37.0 35.0 39.0 30.0 39.0 10-11 35.736643036131156 37.0 35.0 39.0 30.0 39.0 12-13 35.71008251054807 37.0 35.0 39.0 30.0 39.0 14-15 36.48458535906278 38.0 35.0 40.0 30.0 41.0 16-17 36.419857681351864 38.0 35.0 40.0 30.0 41.0 18-19 36.484038412113684 38.0 35.0 40.0 30.0 41.0 20-21 36.403228138185085 38.0 34.5 40.0 30.0 41.0 22-23 36.24505739864213 38.0 34.0 40.0 30.0 41.0 24-25 36.083205007389004 38.0 34.0 40.0 29.0 41.0 26-27 35.61638324302328 38.0 34.0 40.0 27.0 41.0 28-29 35.40630849628408 38.0 34.0 40.0 27.0 41.0 30-31 35.06918919063631 37.5 33.0 40.0 27.0 41.0 32-33 34.77338405688462 37.0 33.0 40.0 26.0 41.0 34-35 34.95692960099377 37.0 33.0 40.0 26.5 41.0 36-37 34.753452753207256 37.0 33.0 40.0 25.0 41.0 38-39 34.552883050266644 37.0 33.0 40.0 25.0 41.0 40-41 34.36499914330385 37.0 33.0 40.0 24.5 41.0 42-43 34.15511983037416 37.0 33.0 40.0 24.0 41.0 44-45 33.68684730461973 37.0 32.5 40.0 23.5 41.0 46-47 33.174432171082216 37.0 31.5 40.0 20.0 41.0 48-49 32.701521171103636 37.0 31.0 39.5 17.0 41.0 50-51 32.43569719003662 36.0 31.0 39.0 15.0 40.5 52-53 32.3076098712814 36.0 31.0 39.0 15.0 40.0 54-55 31.979906994924075 36.0 30.5 39.0 13.0 40.0 56-57 31.500442269388106 35.5 29.5 39.0 9.5 40.0 58-59 31.05546625688034 35.0 29.0 39.0 8.0 40.0 60-61 30.692585027093017 35.0 28.0 38.0 8.0 40.0 62-63 30.544104324173823 34.5 28.0 38.0 7.0 40.0 64-65 30.105396918035595 34.0 28.0 38.0 4.0 40.0 66-67 29.283521984964985 34.0 27.0 37.5 2.0 39.5 68-69 25.950201591313103 31.0 16.5 36.0 2.0 39.0 70-71 24.686162483133792 29.5 12.0 35.0 2.0 38.0 72-73 25.65197040114797 30.5 18.5 35.0 2.0 38.0 74-75 26.36000888822257 31.0 21.5 35.0 2.0 37.5 76-77 25.997545565526547 30.5 22.0 34.0 2.0 36.0 78-79 27.078804534064382 32.0 25.5 35.0 2.0 37.0 80-81 27.08449540596689 32.0 25.0 35.0 2.0 36.5 82-83 26.774760125077638 32.0 25.0 35.0 2.0 36.0 84-85 26.419195348139898 32.0 24.5 35.0 2.0 36.0 86-87 25.97400301985393 32.0 23.5 34.0 2.0 35.0 88-89 25.504240913666447 31.0 21.0 34.0 2.0 35.0 90-91 24.92680040050545 31.0 19.5 34.0 2.0 35.0 92-93 24.374022295517335 31.0 17.5 34.0 2.0 35.0 94-95 23.570884110428132 30.5 7.0 34.0 2.0 35.0 96-97 22.922183611402627 30.0 2.0 34.0 2.0 35.0 98-99 22.09159634619091 30.0 2.0 34.0 2.0 35.0 100-101 20.72620794157332 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.22119419523347972 1101 2 0.2097093150513345 1101 3 0.27188228795876057 1101 4 0.22804267231215647 1101 5 0.5497179391033384 1101 6 0.3688905009073338 1101 7 0.5106683176357834 1101 8 0.5371786492494479 1101 9 0.5646264999841648 1101 10-11 0.6462068970554853 1101 12-13 0.5105809823705059 1101 14-15 0.577226183113666 1101 16-17 0.5783287364375198 1101 18-19 0.4663884358003685 1101 20-21 0.5666549780031929 1101 22-23 0.7988714435480588 1101 24-25 0.8439642220860506 1101 26-27 0.6456909726975013 1101 28-29 0.6162630551325137 1101 30-31 0.5618861911540591 1101 32-33 0.9463694104059144 1101 34-35 0.8043835960307604 1101 36-37 0.9869761624199072 1101 38-39 1.0360456869132904 1101 40-41 1.0364253819989813 1101 42-43 0.6949354079439161 1101 44-45 0.8567904219221916 1101 46-47 0.8038449938678696 1101 48-49 0.8963677216394217 1101 50-51 0.6874828556004573 1101 52-53 0.6043252527119378 1101 54-55 0.44461259088043903 1101 56-57 0.6454337193070323 1101 58-59 0.6796732323399617 1101 60-61 0.5713856385399261 1101 62-63 0.2516409104261612 1101 64-65 0.2564310584549787 1101 66-67 -0.0259796214103325 1101 68-69 -7.077166053024886 1101 70-71 -8.020814252899946 1101 72-73 -4.938211104132876 1101 74-75 -2.8529443694720946 1101 76-77 -0.09918306203215721 1101 78-79 -0.00949731198942061 1101 80-81 -0.08084058912511694 1101 82-83 -0.3634644084254113 1101 84-85 -0.2674544697287935 1101 86-87 -0.43786417155157764 1101 88-89 -0.5616084569511948 1101 90-91 -0.6196159310083544 1101 92-93 -0.7359518557577722 1101 94-95 -0.35195657099043487 1101 96-97 -0.6104873887792124 1101 98-99 -0.6511413033397631 1101 100-101 -0.6439368091872453 1102 1 0.4089451992495441 1102 2 0.3422394355332621 1102 3 0.3582276694848652 1102 4 0.3856731944005105 1102 5 0.5597580997459062 1102 6 0.5320431113490969 1102 7 0.41327875940284997 1102 8 0.5396886894100845 1102 9 0.39394376906047057 1102 10-11 0.5776828006699404 1102 12-13 0.48548058076407585 1102 14-15 0.7727583116277259 1102 16-17 0.5231078529033866 1102 18-19 0.5078040984509684 1102 20-21 0.48432566073412175 1102 22-23 0.38471481704203114 1102 24-25 0.4227794831302276 1102 26-27 0.3869058321352554 1102 28-29 0.4370461876626379 1102 30-31 0.3337235405516452 1102 32-33 0.4235280449440708 1102 34-35 0.3899759655086754 1102 36-37 0.45409463631548874 1102 38-39 0.6550215905277454 1102 40-41 0.5936542976616295 1102 42-43 0.3111502673816702 1102 44-45 0.537764317504525 1102 46-47 0.4903409778036121 1102 48-49 0.5788476413181343 1102 50-51 0.4550531367249562 1102 52-53 0.20648388725008715 1102 54-55 0.014140703330241422 1102 56-57 0.1793192614757011 1102 58-59 0.21230375442831573 1102 60-61 0.1853414618330973 1102 62-63 0.17207263733378753 1102 64-65 0.1331880865674293 1102 66-67 -1.2620775571292597E-4 1102 68-69 -6.943631916478701 1102 70-71 -7.645312244867821 1102 72-73 -1.842076565980264 1102 74-75 -0.3541993895524165 1102 76-77 0.15056593395177842 1102 78-79 -0.14177642845528382 1102 80-81 -0.39133255699660197 1102 82-83 -0.4370085851322365 1102 84-85 -0.7659484456324073 1102 86-87 -0.4363581474551914 1102 88-89 -0.5274719107664545 1102 90-91 -0.6015436418517304 1102 92-93 -0.6363032613802595 1102 94-95 -0.48398468344023726 1102 96-97 -0.5547644972129468 1102 98-99 -0.5663019459100482 1102 100-101 -0.6856034758539131 1103 1 0.3637269293754848 1103 2 0.2910914243530378 1103 3 0.27939631687676325 1103 4 0.2433462372274775 1103 5 0.47712913805163026 1103 6 0.45419675750243726 1103 7 0.43495994748690237 1103 8 0.38937202693698225 1103 9 0.5155072449163001 1103 10-11 0.5130947983449516 1103 12-13 0.5433150024722764 1103 14-15 0.5564805000414097 1103 16-17 0.5303457652131343 1103 18-19 0.6076616393479313 1103 20-21 0.5722294858451562 1103 22-23 0.5052904903033166 1103 24-25 0.5832176174060422 1103 26-27 0.7499696707827042 1103 28-29 0.634217217581245 1103 30-31 0.6535294762907213 1103 32-33 0.801115373373861 1103 34-35 0.7789157703813956 1103 36-37 0.9272652449276961 1103 38-39 0.9686776231087748 1103 40-41 0.9179836695444976 1103 42-43 0.679120448250849 1103 44-45 1.1568120236277082 1103 46-47 0.998690792799394 1103 48-49 0.9053193855071342 1103 50-51 0.5594431347939661 1103 52-53 0.6550723157577139 1103 54-55 0.463625383186141 1103 56-57 0.6948545692926906 1103 58-59 0.8295997962936887 1103 60-61 0.7818639042009181 1103 62-63 0.49034134109492555 1103 64-65 0.48190873409195945 1103 66-67 0.426622555144359 1103 68-69 -6.610401959253004 1103 70-71 -7.885959754771445 1103 72-73 -1.2058441667378332 1103 74-75 0.47194869477128165 1103 76-77 0.6767939640907912 1103 78-79 0.4607180565268969 1103 80-81 0.3098422246945134 1103 82-83 0.2723052364484033 1103 84-85 -0.05882246721656159 1103 86-87 0.11902584602235677 1103 88-89 0.2691744854059017 1103 90-91 -0.023724594901420204 1103 92-93 0.1172368826831196 1103 94-95 0.36592369217002485 1103 96-97 0.3684711847085218 1103 98-99 0.4075402416844298 1103 100-101 0.4745067318540457 1104 1 0.2474709803565105 1104 2 0.2900008984557587 1104 3 0.19638680457030944 1104 4 0.21190635205537944 1104 5 0.4711880094915841 1104 6 0.4254085225574613 1104 7 0.5063192044030274 1104 8 0.5477656139852058 1104 9 0.5585712411213635 1104 10-11 0.5088967447099222 1104 12-13 0.45471907694082603 1104 14-15 0.6224288171220564 1104 16-17 0.5343431432402852 1104 18-19 0.4349250458681908 1104 20-21 0.46830425211224025 1104 22-23 0.6010138151683933 1104 24-25 0.636877144386311 1104 26-27 0.6525252934918342 1104 28-29 0.5236615628393864 1104 30-31 0.416110800551877 1104 32-33 0.5991086408485344 1104 34-35 0.33851089085506914 1104 36-37 0.7701953631802709 1104 38-39 0.7847047265636462 1104 40-41 0.7935080287839895 1104 42-43 0.6234216613961294 1104 44-45 0.8862764552894191 1104 46-47 0.6990317057980349 1104 48-49 0.756376865878849 1104 50-51 0.7127563550297964 1104 52-53 0.5184115201872643 1104 54-55 0.4174386672518864 1104 56-57 0.5659211978341325 1104 58-59 0.45668233567863226 1104 60-61 0.5463283287965872 1104 62-63 0.14672098825444735 1104 64-65 0.13893245102817886 1104 66-67 0.11588779977781627 1104 68-69 -6.722379301752095 1104 70-71 -4.245801739217672 1104 72-73 0.12091166239395079 1104 74-75 0.6508038922459711 1104 76-77 0.890796010416711 1104 78-79 0.4802977070781189 1104 80-81 0.19385007459669623 1104 82-83 0.0018596423850105737 1104 84-85 -0.061777250668264116 1104 86-87 0.10286616005613425 1104 88-89 0.23627828886816715 1104 90-91 0.09278702708980191 1104 92-93 0.025546854894731297 1104 94-95 0.3002546015367251 1104 96-97 -0.07085553449495663 1104 98-99 -0.13588824454785708 1104 100-101 0.04479654323310811 1105 1 0.14636824717817376 1105 2 0.29349693793846754 1105 3 0.2744395645929032 1105 4 0.24304266267206032 1105 5 0.3734302149951887 1105 6 0.24349549794781922 1105 7 0.3591761270780651 1105 8 0.5030833144778057 1105 9 0.42015615581343724 1105 10-11 0.42103954497794405 1105 12-13 0.30184674151846735 1105 14-15 0.39487869426244515 1105 16-17 0.5046332102135622 1105 18-19 0.5798350209889378 1105 20-21 0.45756282446345153 1105 22-23 0.5929294566855816 1105 24-25 0.661550578071683 1105 26-27 0.628537866754769 1105 28-29 0.568818235045633 1105 30-31 0.5740966990521912 1105 32-33 0.6756698334133588 1105 34-35 0.7376681022746538 1105 36-37 0.7088184041665357 1105 38-39 0.6245504500850387 1105 40-41 0.765391173152075 1105 42-43 0.6568894410711437 1105 44-45 0.7671073979395615 1105 46-47 0.9287549794655661 1105 48-49 0.8630938436020728 1105 50-51 0.5299447579385657 1105 52-53 0.4403068410913775 1105 54-55 0.47687739180280886 1105 56-57 0.5672093961864135 1105 58-59 0.6156552786239935 1105 60-61 0.6146076142970109 1105 62-63 0.34710497985799194 1105 64-65 0.368016863991091 1105 66-67 0.4482833691018868 1105 68-69 -3.2302666937057083 1105 70-71 -2.953628201926975 1105 72-73 0.8746475074618445 1105 74-75 1.180011746880016 1105 76-77 0.9483627380669439 1105 78-79 0.464791947786118 1105 80-81 0.4325467335694029 1105 82-83 0.22632803065399898 1105 84-85 0.10260714976888252 1105 86-87 0.18428260084766634 1105 88-89 0.10659375660779702 1105 90-91 0.16853451041167133 1105 92-93 0.31069886136901914 1105 94-95 0.47740841656419875 1105 96-97 0.26303020536115085 1105 98-99 0.564815177407997 1105 100-101 0.6833404949404489 1106 1 -0.02145227999550059 1106 2 0.0606407086097569 1106 3 0.13245898208705498 1106 4 0.12411312011126086 1106 5 0.22659739368054233 1106 6 0.16033096632632038 1106 7 0.1826546820658379 1106 8 0.23419001487003044 1106 9 0.34901702651717414 1106 10-11 0.32023800171126027 1106 12-13 0.04849747045723518 1106 14-15 0.17810598691384882 1106 16-17 0.2245995906051803 1106 18-19 0.06795478022947066 1106 20-21 0.1948059216989293 1106 22-23 0.07926078748507592 1106 24-25 0.2408908614498273 1106 26-27 0.4018242012639348 1106 28-29 0.31015621671163984 1106 30-31 0.25110948750150186 1106 32-33 0.35518748904457453 1106 34-35 0.1648710001928677 1106 36-37 0.41659880318057674 1106 38-39 0.5439121329345298 1106 40-41 0.3884887739065306 1106 42-43 0.48442042310336575 1106 44-45 0.3943864582413852 1106 46-47 0.4617007250931664 1106 48-49 0.5910377059243856 1106 50-51 0.5359557987564756 1106 52-53 0.29148694295115263 1106 54-55 0.22421419889352023 1106 56-57 0.46101422314054474 1106 58-59 0.7249313398688919 1106 60-61 0.7229216243805219 1106 62-63 0.17266691166431514 1106 64-65 0.45672730272058004 1106 66-67 0.36835959315544287 1106 68-69 -2.6107470171977845 1106 70-71 -2.76889848706983 1106 72-73 0.808818516325239 1106 74-75 1.2760528446809616 1106 76-77 1.2296550175589367 1106 78-79 0.7984052468625897 1106 80-81 0.5527858137194634 1106 82-83 0.29100723308132714 1106 84-85 0.23947244742599239 1106 86-87 0.33308127029485846 1106 88-89 0.12896014155349889 1106 90-91 0.4180336792905486 1106 92-93 0.2910295878030347 1106 94-95 0.47014606881780807 1106 96-97 0.46254386667739666 1106 98-99 0.5728455531772099 1106 100-101 0.5780449915334245 1107 1 -0.02070113319513922 1107 2 0.02640031386847852 1107 3 0.14474680170869192 1107 4 -0.10675965793205222 1107 5 0.31080367693675726 1107 6 0.18673538773008858 1107 7 -0.01618974179712751 1107 8 0.08261503604096276 1107 9 0.3270701531719311 1107 10-11 0.09510207636781587 1107 12-13 0.1780694258584532 1107 14-15 0.11835734096131034 1107 16-17 0.2128158822107764 1107 18-19 0.33948443243114923 1107 20-21 0.22549807744562855 1107 22-23 0.2604872242826346 1107 24-25 0.24091416435386748 1107 26-27 0.4441326067701681 1107 28-29 0.34231893444504635 1107 30-31 0.438908706705476 1107 32-33 0.15376635896281243 1107 34-35 0.08123737340820725 1107 36-37 0.250555819734565 1107 38-39 0.308490206587571 1107 40-41 0.3217533471460712 1107 42-43 0.10923573519370677 1107 44-45 0.42704560220177257 1107 46-47 0.5075893491112211 1107 48-49 0.2061866511457069 1107 50-51 0.14002470891436047 1107 52-53 0.012563700733828398 1107 54-55 0.0033715857157829987 1107 56-57 0.3265321723263277 1107 58-59 0.45806326008768394 1107 60-61 0.4440996506344632 1107 62-63 0.27985146967268193 1107 64-65 0.05831420569521839 1107 66-67 0.25034129194706267 1107 68-69 -2.705364244687676 1107 70-71 -2.7839167129156266 1107 72-73 0.9677878288796578 1107 74-75 1.2348251733252873 1107 76-77 0.7932069949342946 1107 78-79 0.5398895228562317 1107 80-81 0.24580203254067357 1107 82-83 0.30667248636902045 1107 84-85 0.17526724502787516 1107 86-87 0.20134797054414832 1107 88-89 0.27986342656884133 1107 90-91 0.331301386026972 1107 92-93 0.29808305458102424 1107 94-95 0.6210730285920398 1107 96-97 0.5233218068593395 1107 98-99 0.7445145765772949 1107 100-101 1.0333993543328681 1108 1 -0.15336927604293038 1108 2 -0.14835138625190325 1108 3 0.03364475283803969 1108 4 0.010970827621143542 1108 5 -0.28822474070680926 1108 6 -0.26308800600569526 1108 7 0.06951238957572059 1108 8 0.11561293977121778 1108 9 0.03083520833536113 1108 10-11 0.003044325821932148 1108 12-13 -0.1567890908075853 1108 14-15 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0.6069297545415466 2316 98-99 0.9112293915610508 2316 100-101 0.7981481975061975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 24.0 4 78.0 5 149.0 6 429.0 7 954.0 8 2151.0 9 3755.0 10 5791.0 11 8731.0 12 11484.0 13 14540.0 14 18210.0 15 22964.0 16 27590.0 17 30786.0 18 26989.0 19 21855.0 20 20932.0 21 21245.0 22 22060.0 23 24553.0 24 28991.0 25 36422.0 26 44870.0 27 55856.0 28 68356.0 29 81483.0 30 95593.0 31 110431.0 32 128161.0 33 147788.0 34 169475.0 35 189333.0 36 193622.0 37 159644.0 38 67909.0 39 4432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.40256930416286 20.656721341676324 15.79241633266855 21.14829302149227 2 15.993327210361569 21.99037640210252 39.032766824560795 22.98352956297512 3 19.282276386023547 26.43873749951141 31.02793009524921 23.25105601921583 4 12.814543396578307 18.15180616093196 36.82223076135674 32.211419681132995 5 12.77494592105545 38.976783534299976 33.55470004926003 14.69357049538455 6 30.290366451778716 37.189394101647 17.38632712942537 15.133912317148916 7 27.153198689254886 34.911760296416865 20.169411663918098 17.76562935041014 8 26.913056049345695 34.870799511683195 19.18496069906406 19.03118373990705 9 26.825351780857126 16.670396864492087 20.58394551412478 35.92030584052601 10-11 24.92613245716807 27.890659972626974 26.198774639470702 20.984432930734254 12-13 26.14904371292112 25.450675719089332 27.499357477886534 20.900923090103017 14-15 23.029866569574438 26.140342892634553 27.078933841639717 23.750856696151292 16-17 21.081080212804235 29.64671305696197 27.59526502008961 21.67694171014418 18-19 20.69413344556065 27.06573757869566 31.691660803646105 20.548468172097593 20-21 22.054410914308967 25.929247606605127 31.807307618170526 20.20903386091538 22-23 21.61832796782031 26.205112073408248 31.58803568679682 20.58852427197462 24-25 21.128644094070363 26.403897707141326 31.96936965060551 20.498088548182803 26-27 21.034309104553493 26.576229856462863 31.738271027174147 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21.623566181847597 27.84839015389956 29.32783922914679 21.200204435106052 54-55 21.261637497981123 27.7478342783325 29.46910858486364 21.52141963882274 56-57 21.350057347203776 28.25911670577738 29.130344316199867 21.260481630818973 58-59 21.53808870941828 27.677509880999562 29.25512258866122 21.52927882092094 60-61 21.495869366264873 27.74620071126048 29.593218124343668 21.164711798130973 62-63 21.552287971034552 27.60670152593871 29.561237403315317 21.27977309971142 64-65 21.279694054883887 27.55455149316649 29.470135465424953 21.695618986524675 66-67 21.052809390957876 27.966762701616414 29.369909734297345 21.610518173128355 68-69 21.20530020605681 27.622047269572025 29.714998789328618 21.457653735042545 70-71 20.744791751062717 27.665456764917167 30.056201016533524 21.53355046748659 72-73 21.29250536459499 27.794123138914735 29.197877858374692 21.715493638115586 74-75 21.171375565654493 27.98692626281512 28.933170402696177 21.90852776883421 76-77 20.610935192747963 28.161038396697474 29.43945301236167 21.78857339819289 78-79 21.050720631009664 28.181496141738332 28.883906206447456 21.883877020804544 80-81 20.876976123869625 28.477682172591056 28.73973301763007 21.905608685909254 82-83 21.16351036923821 28.526053676466063 28.345502594083204 21.964933360212527 84-85 21.0315114599296 28.228998339078558 28.35760998651773 22.381880214474112 86-87 21.089875974977605 28.147035216723857 28.666095168844407 22.096993639454123 88-89 21.12438993700589 28.121849290680462 28.42054255783384 22.33321821447981 90-91 20.87011930004926 28.380105592323673 28.566739488958852 22.18303561866821 92-93 20.90666990797348 28.29125390650204 28.478905223635447 22.323170961889033 94-95 20.63466759831767 28.13278813173788 28.487569264304707 22.744975005639738 96-97 20.876807656533675 28.05328142705138 28.300100511287233 22.76981040512771 98-99 20.682800658140472 27.98968609805436 28.182924494579982 23.144588749225186 100-101 20.577991315109237 27.976454724558085 28.551859529469347 22.89369443086333 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3257.0 1 2242.0 2 1268.0 3 1336.0 4 1229.0 5 1185.5 6 1870.0 7 3453.0 8 5144.0 9 6049.0 10 5660.0 11 4814.5 12 4557.5 13 4524.0 14 4608.0 15 4872.5 16 5119.5 17 5343.0 18 5666.0 19 6013.0 20 6462.5 21 7218.0 22 8203.0 23 9241.0 24 10398.5 25 12069.5 26 14723.0 27 17474.5 28 19597.5 29 22231.5 30 25122.0 31 28182.0 32 32232.5 33 36357.5 34 40674.5 35 44604.5 36 48442.5 37 52474.0 38 54201.5 39 53853.5 40 53682.5 41 55286.5 42 57551.0 43 59435.5 44 61188.5 45 62498.0 46 63094.5 47 64297.0 48 69176.5 49 70535.0 50 66586.5 51 63265.0 52 59903.5 53 58179.5 54 56372.0 55 51705.5 56 46282.5 57 41403.0 58 36984.0 59 32583.5 60 27778.0 61 22363.0 62 17353.5 63 13296.0 64 9953.5 65 7358.5 66 5304.5 67 3981.5 68 2827.5 69 1861.5 70 1325.0 71 1020.5 72 796.5 73 645.0 74 493.0 75 374.5 76 252.5 77 133.5 78 71.5 79 36.0 80 20.5 81 11.0 82 5.5 83 5.0 84 3.5 85 3.5 86 3.0 87 2.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019489837441905293 2 0.019007945856803238 3 6.96065622925189E-4 4 0.09214837977340387 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0057826990212246476 12-13 0.0 14-15 0.0 16-17 4.2834807564627017E-4 18-19 1.0708701891156754E-4 20-21 0.0 22-23 1.3385877363945943E-4 24-25 0.004310252511190593 26-27 0.003988991454455891 28-29 0.0012850442269388106 30-31 1.3385877363945943E-4 32-33 6.692938681972971E-4 34-35 0.002596860208605513 36-37 0.08524126705360777 38-39 0.09747595896425434 40-41 0.078494784862179 42-43 0.08234991754299543 44-45 0.08157353665588658 46-47 0.08010109014585252 48-49 0.07477351095500204 50-51 0.07413098884153263 52-53 0.05531044526782464 54-55 0.047305690604184956 56-57 0.02701270052044291 58-59 0.022488273971429183 60-61 0.013439420873401726 62-63 0.001900794585680324 64-65 0.006184275342143026 66-67 0.15353601336445996 68-69 0.7119680452335568 70-71 0.8700820286564862 72-73 0.35314621661562184 74-75 0.05338287892741642 76-77 0.19342592790901886 78-79 0.0151795849307147 80-81 0.003400012850442269 82-83 0.0013118159816667024 84-85 1.0708701891156754E-4 86-87 0.0017669358120408643 88-89 2.6771754727891885E-4 90-91 0.004283480756462702 92-93 0.004310252511190593 94-95 0.07597823991775718 96-97 0.036704075731939775 98-99 0.013787453684864321 100-101 0.013867768949047996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1867640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.462125106377144 #Duplication Level Percentage of deduplicated Percentage of total 1 76.62517753697445 40.96544827780089 2 13.256469789977226 14.174380927613434 3 4.465819345469477 7.162565776499054 4 1.9176166293308916 4.1007944057342955 5 0.9951596473992886 2.660167478503947 6 0.5955421788656761 1.9103370283584724 7 0.39666220996635043 1.4844483285935148 8 0.2716367712811545 1.161782323978035 9 0.1889138023439333 0.9089760001709489 >10 1.1134694716843136 11.322269720714875 >50 0.10595786339801712 3.936627026923837 >100 0.0627994437633384 5.9586088411170435 >500 0.003454479245943101 1.1858329627215887 >1k 0.0010160233076303238 1.0353699831632175 >5k 1.0160233076303238E-4 0.49272551897977784 >10k+ 2.0320466152606476E-4 1.5396653991270868 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14833 0.7942108757576406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13512 0.7234798997665502 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9071 0.4856931742734146 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3733 0.1998779207984408 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3567 0.1909896982287807 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2695 0.14429975798333727 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1942 0.10398149536313209 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.354350945578377E-5 0.0 5 0.0 0.0 0.0 5.354350945578377E-5 0.0 6 0.0 0.0 0.0 5.354350945578377E-5 0.0 7 0.0 0.0 0.0 5.354350945578377E-5 0.0 8 0.0 0.0 0.0 5.354350945578377E-5 0.0 9 0.0 0.0 0.0 5.354350945578377E-5 0.0 10-11 0.0 0.0 0.0 5.354350945578377E-5 0.0 12-13 0.0 0.0 0.0 5.354350945578377E-5 0.0 14-15 0.0 0.0 0.0 5.354350945578377E-5 0.0 16-17 5.354350945578377E-5 0.0 0.0 5.354350945578377E-5 0.0 18-19 1.3385877363945943E-4 0.0 0.0 5.354350945578377E-5 0.0 20-21 1.6063052836735132E-4 0.0 0.0 1.3385877363945943E-4 0.0 22-23 2.1417403782313509E-4 0.0 0.0 4.2834807564627017E-4 0.0 24-25 2.1417403782313509E-4 0.0 0.0 8.299243965646484E-4 0.0 26-27 2.1417403782313509E-4 0.0 0.0 0.0013385877363945942 0.0 28-29 2.6771754727891885E-4 0.0 0.0 0.0026236319633334048 0.0 30-31 2.6771754727891885E-4 0.0 0.0 0.007014199738707674 0.0 32-33 2.6771754727891885E-4 0.0 0.0 0.015822107044184103 0.0 34-35 3.2126105673470264E-4 0.0 0.0 0.029904050031055236 0.0 36-37 3.748045661904864E-4 0.0 0.0 0.05231200873830075 0.0 38-39 3.748045661904864E-4 0.0 0.0 0.08620505022381186 0.0 40-41 3.748045661904864E-4 0.0 0.0 0.12408708316377888 0.0 42-43 4.283480756462701E-4 0.0 0.0 0.16196911610374592 0.0 44-45 4.818915851020539E-4 0.0 0.0 0.20879291512282883 0.0 46-47 6.425221134694053E-4 0.0 0.0 0.2663254160330685 2.6771754727891886E-5 48-49 6.96065622925189E-4 0.0 0.0 0.332799683022424 5.354350945578377E-5 50-51 6.96065622925189E-4 0.0 0.0 0.4030755391831402 5.354350945578377E-5 52-53 6.96065622925189E-4 0.0 0.0 0.47324430832494485 5.354350945578377E-5 54-55 6.96065622925189E-4 0.0 0.0 0.552943822149879 5.354350945578377E-5 56-57 6.96065622925189E-4 0.0 0.0 0.64627015913131 5.354350945578377E-5 58-59 6.96065622925189E-4 2.6771754727891886E-5 0.0 0.7443886402090338 5.354350945578377E-5 60-61 6.96065622925189E-4 5.354350945578377E-5 0.0 0.8474063524019618 5.354350945578377E-5 62-63 7.228373776530809E-4 5.354350945578377E-5 0.0 0.9492728791415905 8.031526418367566E-5 64-65 7.496091323809728E-4 5.354350945578377E-5 0.0 1.0525583088817974 1.0708701891156754E-4 66-67 8.031526418367565E-4 5.354350945578377E-5 0.0 1.1648925917200317 1.0708701891156754E-4 68-69 8.031526418367565E-4 5.354350945578377E-5 0.0 1.2955119830374162 1.0708701891156754E-4 70-71 8.031526418367565E-4 5.354350945578377E-5 0.0 1.4659409736351758 1.0708701891156754E-4 72-73 8.031526418367565E-4 5.354350945578377E-5 0.0 1.6496219828232421 1.0708701891156754E-4 74-75 8.031526418367565E-4 5.354350945578377E-5 0.0 1.8406652245614787 1.0708701891156754E-4 76-77 8.031526418367565E-4 5.354350945578377E-5 0.0 2.060514874386927 1.0708701891156754E-4 78-79 8.031526418367565E-4 5.354350945578377E-5 0.0 2.317952067850335 1.0708701891156754E-4 80-81 8.031526418367565E-4 5.354350945578377E-5 0.0 2.6077830845344927 1.0708701891156754E-4 82-83 8.031526418367565E-4 5.354350945578377E-5 0.0 2.9025401040885823 1.0708701891156754E-4 84-85 8.031526418367565E-4 5.354350945578377E-5 0.0 3.198796341907434 1.0708701891156754E-4 86-87 8.031526418367565E-4 5.354350945578377E-5 0.0 3.506564434259279 1.0708701891156754E-4 88-89 8.031526418367565E-4 5.354350945578377E-5 0.0 3.8457625666616693 1.0708701891156754E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8870 0.0 44.995758 1 ACCGTAC 55 0.0026062336 34.474358 8 GTATCAA 13950 0.0 33.270844 1 TATCAAC 18005 0.0 25.69385 2 ATCAACG 18075 0.0 25.484562 3 TCAACGC 18345 0.0 25.138037 4 CAACGCA 18675 0.0 24.798925 5 GTACATG 12870 0.0 24.41061 1 AACGCAG 19100 0.0 24.271935 6 TACATGG 12755 0.0 24.030886 2 ACATGGG 12920 0.0 22.875036 3 TGGTATC 4775 0.0 21.761482 2 TACCTGG 1225 0.0 21.690504 2 GAGTACT 12290 0.0 21.596756 12-13 ATACCGT 135 3.8503605E-4 21.067663 6 GTGGTAT 5075 0.0 21.039997 1 ACGCAGA 22000 0.0 21.02936 7 CGCAGAG 21980 0.0 21.026928 8 AGTACTT 12550 0.0 20.45065 12-13 GTACTTT 13015 0.0 20.375502 14-15 >>END_MODULE