FastQCFastQC Report
Fri 27 May 2016
SRR939457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976191
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65380.3308384665247438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.2648023394499823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34420.17417344780944757No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34020.17214935196041273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG87500.025.447511
TACATGG88250.024.5528452
GTATCAA161850.023.3601111
GAGTACT74750.022.572612-13
ACATGGG91700.022.5503673
CATGGGG34000.021.9496674
GTACTTT81750.020.66314314-15
AGTACTT78100.020.04150612-13
TATCAAC186350.020.0150132
ATCAACG190500.019.5740973
TCAACGC191700.019.539884
GGTATCA122800.019.471021
CAACGCA190650.019.4573425
AACGCAG193900.019.391366
CATGGGA61900.019.0119514
ACTTTTT100800.018.1934316-17
AGAGTAC135900.018.05602510-11
GTACCGT1901.6032674E-417.8512556
ACGCAGA208600.017.5452967
CGCAGAG209150.017.3855748
Produced by FastQC (version 0.11.5)