FastQCFastQC Report
Fri 27 May 2016
SRR939457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976191
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90170.4562818067686777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81010.4099300118257799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54260.2745686019215754No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT50470.2553902937519703No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30610.15489393484739075No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT22360.11314695796104729No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.1108698501308831No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20160.1020144307913557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG100850.025.1395281
TACATGG101450.024.6601162
ACATGGG103800.022.9542453
GAGTACT84250.020.55042512-13
AGTACTT87950.020.41299412-13
CATGGGA67700.020.3608864
GTATCAA173550.020.0151371
GGTATCA136200.019.7628881
CGCATAG2200.019.748466-67
GTACTTT89000.019.21412314-15
CATGGGG39750.019.1858544
CGCGTGA650.00689281818.56687266-67
TCGCGCG650.00689281818.56687266-67
TAGGACA9700.018.068514
TACTTTT100250.017.74307614-15
AGAGTAC155600.017.62675310-11
TGCACCG4353.6379788E-1217.4230065
AGTACAT78800.017.1970162
GTGTAGC11100.017.0771831
TACACTG19150.017.0676085
Produced by FastQC (version 0.11.5)