##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939457_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1976191 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.980142607673045 31.0 31.0 31.0 30.0 34.0 2 31.239590201554403 31.0 31.0 34.0 30.0 34.0 3 31.23457094987276 31.0 31.0 34.0 28.0 34.0 4 35.16266747495561 35.0 35.0 37.0 33.0 37.0 5 34.78014473297368 35.0 35.0 37.0 32.0 37.0 6 34.75702804030582 35.0 35.0 37.0 32.0 37.0 7 34.62728552047854 35.0 35.0 37.0 30.0 37.0 8 34.6392403365869 35.0 35.0 37.0 30.0 37.0 9 35.990052074925956 37.0 35.0 39.0 30.0 39.0 10-11 35.84928101585322 37.0 35.0 39.0 30.0 39.0 12-13 35.8159805909449 37.0 35.0 39.0 30.0 39.0 14-15 36.66512093213662 38.0 35.5 40.0 31.0 41.0 16-17 36.683782336828784 38.0 35.5 40.0 31.0 41.0 18-19 36.639996083374534 38.0 35.0 40.0 30.0 41.0 20-21 36.5548066963163 38.0 35.0 40.0 30.0 41.0 22-23 36.41451636000771 38.0 35.0 40.0 30.0 41.0 24-25 36.18837526332222 38.0 34.0 40.0 29.5 41.0 26-27 35.81153213429269 38.0 34.0 40.0 28.0 41.0 28-29 35.53302236474106 38.0 34.0 40.0 27.0 41.0 30-31 35.32107701128079 38.0 34.0 40.0 27.0 41.0 32-33 35.12181641349444 37.5 33.0 40.0 27.0 41.0 34-35 35.1037715990003 38.0 33.0 40.0 27.0 41.0 36-37 35.11773204108307 38.0 33.5 40.0 26.5 41.0 38-39 35.07948244881188 38.0 33.0 40.0 26.0 41.0 40-41 34.874012430984656 38.0 33.0 40.0 25.5 41.0 42-43 34.58024224379122 38.0 33.0 40.0 24.5 41.0 44-45 34.3802142606661 38.0 33.0 40.0 24.0 41.0 46-47 34.05365549180216 37.0 33.0 40.0 24.0 41.0 48-49 33.73351082967183 37.0 32.5 40.0 23.0 41.0 50-51 33.47014306815485 37.0 32.0 40.0 22.5 41.0 52-53 33.11763134231458 37.0 31.0 40.0 20.5 41.0 54-55 32.89644093106385 36.5 31.0 39.0 19.5 41.0 56-57 32.50653049224493 36.0 31.0 39.0 18.5 40.0 58-59 32.08032649678093 36.0 30.5 39.0 17.0 40.0 60-61 31.773581855195168 35.0 30.0 39.0 14.5 40.0 62-63 31.400913929878236 35.0 29.0 38.5 13.0 40.0 64-65 30.955398288930574 34.5 29.0 38.0 9.5 40.0 66-67 29.17271584578616 33.5 25.5 37.5 7.0 39.5 68-69 25.366927842501052 29.5 15.5 35.0 4.0 39.0 70-71 24.727724445663398 29.5 14.0 35.0 2.0 38.0 72-73 26.66944060569044 31.0 21.5 35.0 2.0 38.0 74-75 27.330187972721262 32.0 24.0 35.0 2.0 38.0 76-77 27.424060477959873 31.5 25.5 34.5 2.0 36.5 78-79 28.280890359282076 33.0 26.0 35.0 2.0 37.0 80-81 28.13704469861466 33.0 26.0 35.0 2.0 37.0 82-83 27.734434070390968 33.0 26.0 35.0 2.0 36.0 84-85 27.39459242552972 32.5 26.0 35.0 2.0 36.0 86-87 26.956570493439145 32.0 25.5 35.0 2.0 35.5 88-89 26.46161681740277 32.0 25.0 34.0 2.0 35.0 90-91 25.991955484059993 32.0 24.0 34.0 2.0 35.0 92-93 25.423862116566667 31.5 21.5 34.0 2.0 35.0 94-95 24.006998311398036 31.0 12.5 34.0 2.0 35.0 96-97 23.198238935406547 30.0 4.5 34.0 2.0 35.0 98-99 22.895662666209894 30.0 2.0 34.0 2.0 35.0 100-101 21.63670262641617 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38733488115532566 1101 2 0.3181414816719865 1101 3 0.41898189149246434 1101 4 0.38952664253357483 1101 5 0.36015044307246313 1101 6 0.46507046691579035 1101 7 0.4416574017839636 1101 8 0.5290364090085617 1101 9 0.5673176905768003 1101 10-11 0.9618310174451068 1101 12-13 0.7341647451813671 1101 14-15 0.8254973390946034 1101 16-17 0.8019231293561049 1101 18-19 0.8048206846812391 1101 20-21 0.867710519128849 1101 22-23 0.8208370495866504 1101 24-25 0.7820859726369989 1101 26-27 0.8283969250088674 1101 28-29 0.8429241925836592 1101 30-31 0.9433895717035128 1101 32-33 0.8167295933324894 1101 34-35 0.7635370074959553 1101 36-37 0.842655283942598 1101 38-39 0.6872929362989737 1101 40-41 0.883934418831636 1101 42-43 0.7733314786702508 1101 44-45 0.42470529474589114 1101 46-47 0.5918651155139045 1101 48-49 0.664388623811746 1101 50-51 0.6644678432399331 1101 52-53 0.7615513638621607 1101 54-55 0.6721053559935868 1101 56-57 0.3992835449093448 1101 58-59 0.48090974672908615 1101 60-61 0.21668508962347488 1101 62-63 0.08464664562293223 1101 64-65 -0.043300546941818396 1101 66-67 -2.981659597591875 1101 68-69 -8.057000627783964 1101 70-71 -11.193838366849105 1101 72-73 -11.304262609283377 1101 74-75 -9.70647391532356 1101 76-77 -4.530901730277524 1101 78-79 -1.4618738110801104 1101 80-81 -0.8268090574519107 1101 82-83 -0.7266859652361397 1101 84-85 -0.7214797449968202 1101 86-87 -0.5814283749924947 1101 88-89 -0.5655521276755877 1101 90-91 -0.716965100130075 1101 92-93 -1.2111893298724503 1101 94-95 -4.95501528513525 1101 96-97 -1.7933480301482412 1101 98-99 -0.9744768129960697 1101 100-101 -0.7545836739866978 1102 1 0.45105439546537696 1102 2 0.32627671894730526 1102 3 0.40872832342675736 1102 4 0.22165221332733864 1102 5 0.6059049277306272 1102 6 0.5951519324706425 1102 7 0.44123132594246073 1102 8 0.5723004493393589 1102 9 0.7265116003575258 1102 10-11 0.7503582043332173 1102 12-13 0.7043660888354495 1102 14-15 0.6371319295889819 1102 16-17 0.6279143215980909 1102 18-19 0.8888321978565443 1102 20-21 1.0101756581292491 1102 22-23 1.0139213311851805 1102 24-25 0.824344426518401 1102 26-27 0.9227383128915889 1102 28-29 0.9003177402546712 1102 30-31 0.7081567323917923 1102 32-33 0.47190763790360535 1102 34-35 0.919877450490695 1102 36-37 0.900650613538005 1102 38-39 0.6458800622366923 1102 40-41 0.8245260472263212 1102 42-43 0.7838008229333226 1102 44-45 0.5677566074939548 1102 46-47 0.6130834733584649 1102 48-49 0.5133551434512142 1102 50-51 0.4160638934036953 1102 52-53 0.5106036463252508 1102 54-55 0.4952505660723503 1102 56-57 0.3275891650577947 1102 58-59 0.20558926796150345 1102 60-61 0.04245904525389932 1102 62-63 0.11454890010083574 1102 64-65 0.1779328531689579 1102 66-67 -2.592031313165986 1102 68-69 -7.6241500161077305 1102 70-71 -11.224146160093888 1102 72-73 -9.361205984278676 1102 74-75 -7.703909519272372 1102 76-77 -4.026944372911618 1102 78-79 -1.1429325858145063 1102 80-81 -0.5300718783368588 1102 82-83 -0.34638488989665817 1102 84-85 -0.40458839360619336 1102 86-87 -0.426169094881768 1102 88-89 -0.3955510615732294 1102 90-91 -0.5583779924475287 1102 92-93 -0.8696732958024498 1102 94-95 -4.139716606700567 1102 96-97 -0.9677596383965152 1102 98-99 -0.3141791951871973 1102 100-101 -0.3946701633663814 1103 1 0.45561147523121903 1103 2 0.338190623197967 1103 3 0.43845084928015865 1103 4 0.30092345962286515 1103 5 0.40318996080526404 1103 6 0.45458171251127055 1103 7 0.5182185651714732 1103 8 0.40095727531854664 1103 9 0.7106194088442663 1103 10-11 0.7655650843828496 1103 12-13 0.6095595669956921 1103 14-15 0.7433470967955031 1103 16-17 0.7388319045343579 1103 18-19 0.625466209643065 1103 20-21 0.8443494190740992 1103 22-23 0.7340493689801875 1103 24-25 0.8608588345518058 1103 26-27 0.7206018651103037 1103 28-29 0.7821822904166353 1103 30-31 0.8327635963314535 1103 32-33 0.9113308791926542 1103 34-35 0.871345261718325 1103 36-37 0.7769173144170907 1103 38-39 0.8391227691048968 1103 40-41 1.059537307802131 1103 42-43 1.1237390000212244 1103 44-45 0.921200185977014 1103 46-47 0.9652347918791335 1103 48-49 0.9609799802102259 1103 50-51 0.972357022648282 1103 52-53 1.100980291537475 1103 54-55 1.132021366792408 1103 56-57 1.1817877615953236 1103 58-59 1.1932309728042974 1103 60-61 1.0952751368518179 1103 62-63 0.9636761371356819 1103 64-65 0.7851175342663161 1103 66-67 -2.2817408526359557 1103 68-69 -7.463952607042486 1103 70-71 -11.063978182700327 1103 72-73 -8.93095157435727 1103 74-75 -7.2658252823654585 1103 76-77 -3.5079974836844094 1103 78-79 -0.46895678065326507 1103 80-81 0.1736439476652869 1103 82-83 0.30874097742646 1103 84-85 0.10812399280851182 1103 86-87 0.3693373722497064 1103 88-89 0.48353552148440926 1103 90-91 0.326293956490435 1103 92-93 0.3806594734390316 1103 94-95 1.3355691720979124 1103 96-97 1.5832764293426216 1103 98-99 1.4698008087710441 1103 100-101 1.2712394502385926 1104 1 0.30342125887462856 1104 2 0.22499325629768663 1104 3 0.2262221740177779 1104 4 0.14480094919366593 1104 5 0.3581346532181797 1104 6 0.3400778200525778 1104 7 0.39836925136857104 1104 8 0.3013476994175619 1104 9 0.39986550282658584 1104 10-11 0.518770055538667 1104 12-13 0.36270263434964534 1104 14-15 0.5123864433008194 1104 16-17 0.5398814077516718 1104 18-19 0.5154181752851485 1104 20-21 0.4963241877577289 1104 22-23 0.4149834281527376 1104 24-25 0.5405539760945643 1104 26-27 0.7307983189762624 1104 28-29 0.84114070564938 1104 30-31 0.7717942426408015 1104 32-33 0.6009603653013258 1104 34-35 0.4968538173474002 1104 36-37 0.6721461583720085 1104 38-39 0.6619898090895191 1104 40-41 0.7104211856208593 1104 42-43 0.9977651372651408 1104 44-45 0.7848564077953526 1104 46-47 0.7102406886370645 1104 48-49 0.6913084107375127 1104 50-51 0.6855970115317831 1104 52-53 0.6914828082730011 1104 54-55 0.4776004628323207 1104 56-57 0.6643952245381328 1104 58-59 0.7407337248116868 1104 60-61 0.5905104143136413 1104 62-63 0.7045051169423253 1104 64-65 0.5448554137450365 1104 66-67 -2.8536284660031406 1104 68-69 -7.524116369716403 1104 70-71 -8.998311059828197 1104 72-73 -8.295152025974492 1104 74-75 -7.328043185244187 1104 76-77 -3.752573794240263 1104 78-79 -0.8624182804159375 1104 80-81 -0.15975384541742343 1104 82-83 -0.0665881319854229 1104 84-85 0.07390325320208291 1104 86-87 0.2727643554997705 1104 88-89 0.3448058286432172 1104 90-91 0.2723697604076172 1104 92-93 0.300791693227076 1104 94-95 1.0662829584673332 1104 96-97 1.3736551010703124 1104 98-99 0.9485237613916304 1104 100-101 0.8326922520495508 1105 1 0.44940268225036917 1105 2 0.3308503409514856 1105 3 0.4229144688478961 1105 4 0.29935043229971825 1105 5 0.374468486083515 1105 6 0.48565024996828754 1105 7 0.5386053371520063 1105 8 0.41229443685082856 1105 9 0.6866872782350057 1105 10-11 0.8016193544014101 1105 12-13 0.8099784906257312 1105 14-15 0.9861941802638583 1105 16-17 0.8885223287641182 1105 18-19 0.9113131487476736 1105 20-21 0.9593324563337617 1105 22-23 0.9877375555718615 1105 24-25 1.1078129898472682 1105 26-27 1.1415291180678437 1105 28-29 1.2225857870908925 1105 30-31 0.965182011616804 1105 32-33 0.9547628662060532 1105 34-35 1.1423628572018885 1105 36-37 1.095427895204729 1105 38-39 0.8972700677366703 1105 40-41 1.0487332554810251 1105 42-43 1.0997682828252024 1105 44-45 0.9002062031808791 1105 46-47 0.9684966062857185 1105 48-49 0.9607707295655743 1105 50-51 0.9919409632564182 1105 52-53 1.271089931196343 1105 54-55 1.2730854811899306 1105 56-57 1.1742549303611511 1105 58-59 1.3196502296995867 1105 60-61 1.257504857968705 1105 62-63 1.352698617949894 1105 64-65 1.2646891041299178 1105 66-67 1.9444554467375958 1105 68-69 -5.230351714444506 1105 70-71 -5.444510462587846 1105 72-73 -1.3472301994615101 1105 74-75 1.4754078835161657 1105 76-77 1.4113880335601436 1105 78-79 1.3388845129073417 1105 80-81 1.33990241559734 1105 82-83 1.5191860785041165 1105 84-85 1.3015190058089239 1105 86-87 1.534553042473764 1105 88-89 1.5343924508675002 1105 90-91 1.583213817716942 1105 92-93 1.5933769597283138 1105 94-95 2.3303342584934725 1105 96-97 2.54988732143379 1105 98-99 2.4012598962385816 1105 100-101 2.063897785786015 1106 1 0.214857386635245 1106 2 0.12657123984690344 1106 3 0.18720488158269788 1106 4 0.007035595384373039 1106 5 0.3583448229034687 1106 6 0.2832596802117706 1106 7 0.31760226616813725 1106 8 0.1517760975609903 1106 9 0.4093196390768128 1106 10-11 0.3259849611382535 1106 12-13 0.33679781108174467 1106 14-15 0.5008216668068499 1106 16-17 0.3848621143300974 1106 18-19 0.3929837376309848 1106 20-21 0.4489745713611484 1106 22-23 0.4907809987778151 1106 24-25 0.5563155359619216 1106 26-27 0.5304992208783474 1106 28-29 0.7700293746259064 1106 30-31 0.6424447168446576 1106 32-33 0.5631042555169259 1106 34-35 0.8120325208784678 1106 36-37 0.6665333057083487 1106 38-39 0.611965827192499 1106 40-41 0.7266909633950931 1106 42-43 0.7405961831966437 1106 44-45 0.6700443638475306 1106 46-47 0.549540106169566 1106 48-49 0.5190906850534702 1106 50-51 0.47118009048072906 1106 52-53 0.4071482072464221 1106 54-55 0.5358104664816139 1106 56-57 0.6762329688749773 1106 58-59 0.6973840399787186 1106 60-61 0.4531568453271433 1106 62-63 0.6956925717757692 1106 64-65 0.5788064696775876 1106 66-67 1.3221029429717106 1106 68-69 -5.23384957864587 1106 70-71 -6.241594524684466 1106 72-73 -2.34773770608923 1106 74-75 0.27161603267961 1106 76-77 0.7473596163821163 1106 78-79 0.7689063738102107 1106 80-81 0.6791907528036596 1106 82-83 0.7293059961535349 1106 84-85 0.7634968208042991 1106 86-87 0.8525781890978763 1106 88-89 0.705325093582033 1106 90-91 0.6546586968692978 1106 92-93 0.8484357610467512 1106 94-95 1.8473580823673217 1106 96-97 1.8026858921929474 1106 98-99 1.5409976452321494 1106 100-101 1.3356976202227067 1107 1 0.03809804116801274 1107 2 -0.0358793111243898 1107 3 -0.0761820790226686 1107 4 -0.03807612605187671 1107 5 0.12908741609200547 1107 6 0.2432332964783015 1107 7 0.19810307397549565 1107 8 0.10785090715510393 1107 9 0.17800074719361447 1107 10-11 0.11824212966087799 1107 12-13 0.1966369906802825 1107 14-15 0.14776911306288554 1107 16-17 0.12537846413811593 1107 18-19 0.22028840424360396 1107 20-21 0.11439063104636205 1107 22-23 0.24376001871465292 1107 24-25 0.35705212279773235 1107 26-27 0.3774330330821769 1107 28-29 0.19379434649390248 1107 30-31 0.17131912142963301 1107 32-33 0.05340033144835843 1107 34-35 0.2574339761600015 1107 36-37 0.29634432464288807 1107 38-39 0.1578891432232794 1107 40-41 0.010010867391713418 1107 42-43 0.1456897709340197 1107 44-45 0.2511994219171285 1107 46-47 0.0446729951883853 1107 48-49 0.2867694037309789 1107 50-51 0.2566784744565851 1107 52-53 0.5548590375970477 1107 54-55 0.2549842019744517 1107 56-57 0.44058567484538713 1107 58-59 0.17451721641809215 1107 60-61 0.31794522682184123 1107 62-63 0.25307504256082325 1107 64-65 0.354683020499845 1107 66-67 1.1346589838394756 1107 68-69 -5.599299907705564 1107 70-71 -6.022604930782691 1107 72-73 -2.182001369006329 1107 74-75 0.26563547410109223 1107 76-77 0.284346793822138 1107 78-79 0.45476030766418063 1107 80-81 0.33230945012524415 1107 82-83 0.38597896396325027 1107 84-85 0.30851642026614456 1107 86-87 0.4625136873385536 1107 88-89 0.3787110347042173 1107 90-91 0.38931629382051014 1107 92-93 0.4021525961123906 1107 94-95 1.3920603728062062 1107 96-97 1.6325527779870868 1107 98-99 1.3945592240635882 1107 100-101 1.3491613491400791 1108 1 0.08782871988282182 1108 2 -0.012224134660218766 1108 3 0.11066061133755767 1108 4 0.05005506949131444 1108 5 0.0936278289674135 1108 6 0.20546413250694684 1108 7 0.1400628687543488 1108 8 0.0518624938434229 1108 9 0.22386905663622514 1108 10-11 0.39314029517181126 1108 12-13 0.2879164555615503 1108 14-15 0.33254176310882855 1108 16-17 0.1474256834587706 1108 18-19 0.2889865168970971 1108 20-21 0.3031817056886297 1108 22-23 0.3762965050367413 1108 24-25 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1.5509917853797965 2316 100-101 1.5167366299567355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 26.0 4 62.0 5 136.0 6 364.0 7 900.0 8 1831.0 9 2975.0 10 4714.0 11 7236.0 12 9628.0 13 12117.0 14 14831.0 15 18591.0 16 22419.0 17 25436.0 18 23625.0 19 20442.0 20 19979.0 21 20449.0 22 21416.0 23 24218.0 24 29155.0 25 36764.0 26 46180.0 27 57674.0 28 71066.0 29 85161.0 30 100727.0 31 117658.0 32 134952.0 33 156472.0 34 183616.0 35 209485.0 36 223574.0 37 188954.0 38 78443.0 39 4911.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.51570627496851 20.423244163323194 15.465706755801248 22.595342805907055 2 15.89816340660542 21.586855562618684 38.625095655905966 23.88988537486993 3 19.63949840880765 25.09479093873011 30.411230493408787 24.854480159053452 4 13.243659140234925 17.545773662566017 36.7689155552272 32.44165164197185 5 13.38633439041953 38.232616650144955 33.852767981977415 14.528280977458104 6 31.367210962907937 36.72337339862392 17.073805112967317 14.835610525500826 7 27.935204643680695 33.44610920705539 20.448681326855553 18.170004822408362 8 26.29568700596248 34.96757145437865 19.384057512659457 19.352684026999416 9 26.100968985285327 17.063988248099502 20.618907787759383 36.21613497885579 10-11 25.00416204708958 27.108108477368837 26.743670019750116 21.14405945579147 12-13 26.125258135473743 24.375022454813326 27.64054689045745 21.859172519255477 14-15 22.7233602420009 25.75262208966643 26.63996040868519 24.884057259647474 16-17 21.619747958692106 29.15788711982285 27.201136129024423 22.02122879246062 18-19 21.425083444153646 27.61485336296638 29.741540716091514 21.218522476788458 20-21 22.469898524459055 26.360869561917234 29.721426147934626 21.447805765689086 22-23 21.98484357028243 26.475148404177528 29.733588504350035 21.80641952119001 24-25 22.05019656500814 26.60595559842141 29.848886064150683 21.49496177241977 26-27 21.962144728895506 26.626739557971433 29.99385937902053 21.417256334112526 28-29 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27.578771874605135 28.20880506903119 21.928502715896077 54-55 21.798891643575907 27.280668173642507 28.471943715262586 22.448496467519 56-57 22.13565270566581 27.57885097317304 28.161815756096363 22.123680565064788 58-59 22.47288024592761 27.191773126315244 28.167446577854978 22.16790004990217 60-61 22.22468510335624 27.250278402565176 28.758573799516245 21.76646269456234 62-63 22.118257337001072 27.229994919408867 28.657393069749837 21.99435467384022 64-65 21.906894282373663 27.48260027220165 28.384676246268864 22.225829199155818 66-67 21.489407390748035 27.347063764038875 28.82315494954962 22.340373895663465 68-69 21.377122785100173 27.03704951179196 29.418636161127793 22.167191541980074 70-71 21.115844136738183 27.11552027723191 29.56745344775007 22.201182138279837 72-73 21.947158960576026 27.12232983299467 28.53551629149505 22.39499491493426 74-75 21.481913019504017 27.78139019589161 28.292785277416815 22.443911507187558 76-77 21.823898416168795 27.564533719991598 27.928063343126315 22.683504520713292 78-79 21.824495241825677 27.496501174962507 28.129667683103488 22.549335900108325 80-81 21.832234780525166 27.726695276016294 27.952714367076965 22.488355576381576 82-83 21.85180987060461 27.588249313958013 28.041191362575784 22.51874945286159 84-85 21.670400280134867 27.658358933928955 27.85917454335133 22.81206624258485 86-87 21.766965137373273 27.532543764880312 27.978858254667454 22.72163284307896 88-89 21.700893491935698 27.559725105873508 27.68347401728285 23.055907384907943 90-91 21.667622659535894 27.477461635251316 27.91670388165154 22.938211823561247 92-93 21.817807354765122 27.58340540067333 27.80681837158005 22.791968872981503 94-95 21.207032272331656 27.357988147673257 28.24128269686082 23.193696883134272 96-97 20.886704379881383 27.31836724859653 28.42353943719304 23.371388934329044 98-99 21.354460835221072 27.44406622170668 27.59212473455242 23.60934820851983 100-101 21.235343235242023 27.07775612965259 28.003668935502645 23.683231699602743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1238.0 1 856.0 2 516.0 3 572.5 4 560.0 5 576.0 6 882.0 7 1662.0 8 2456.5 9 2905.0 10 2920.0 11 2677.0 12 2659.5 13 2830.5 14 2942.0 15 3016.0 16 3193.5 17 3439.5 18 3644.5 19 3925.0 20 4439.5 21 5043.5 22 5934.0 23 7010.5 24 8223.5 25 10093.5 26 12491.0 27 15280.0 28 18155.0 29 21362.0 30 24867.5 31 28167.5 32 31762.0 33 35666.5 34 40036.5 35 43782.0 36 47448.5 37 51143.5 38 53672.5 39 55703.5 40 57535.5 41 60249.5 42 62985.5 43 65491.5 44 68269.5 45 70837.0 46 73738.5 47 79139.5 48 87109.0 49 85598.5 50 76195.5 51 70190.5 52 66491.0 53 64324.0 54 61560.5 55 57188.0 56 52131.0 57 46822.0 58 41709.0 59 36264.0 60 30297.0 61 24349.5 62 19212.5 63 14941.0 64 11323.5 65 8546.0 66 6311.5 67 4651.5 68 3172.5 69 1909.0 70 1236.0 71 891.5 72 697.0 73 530.0 74 399.5 75 292.5 76 201.0 77 108.0 78 59.0 79 34.0 80 20.5 81 18.5 82 13.0 83 4.0 84 2.0 85 3.0 86 2.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02287228309409364 2 0.018166260245087645 3 0.0 4 0.0 5 2.0240958490348355E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.542167735810962E-4 18-19 9.614455282915467E-4 20-21 2.5301198112935444E-5 22-23 0.0 24-25 0.0 26-27 3.289155754681607E-4 28-29 0.017356621905473713 30-31 0.003972288103730864 32-33 0.0077927690187841155 34-35 0.008880720537640338 36-37 0.10540479133848905 38-39 0.10717587520639453 40-41 0.12197707610246175 42-43 0.10778310396110496 44-45 0.10254575595172735 46-47 0.10429153862151988 48-49 0.10629033327244179 50-51 0.10411443023472934 52-53 0.08159636391421679 54-55 0.06957829481057246 56-57 0.03903974868825938 58-59 0.016445778773408037 60-61 0.009386744499899048 62-63 0.0021759030377124476 64-65 0.004174697688634347 66-67 0.4383938597028324 68-69 0.8439467642550746 70-71 0.9387756547823565 72-73 0.28919269443085205 74-75 0.15479273005493902 76-77 0.014472285320599071 78-79 0.008627708556510984 80-81 0.001543373084889062 82-83 0.0 84-85 0.0 86-87 3.0361437735522526E-4 88-89 6.072287547104505E-4 90-91 0.003061444971665188 92-93 0.0015180718867761264 94-95 0.23251801065787672 96-97 0.23952644253515978 98-99 0.01432047813192146 100-101 0.007134937867847794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1976191.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.27823445143921 #Duplication Level Percentage of deduplicated Percentage of total 1 76.06550288477183 42.04766702131043 2 13.480422606932601 14.903479227410035 3 4.525731167491249 7.50523285622301 4 2.080914109382751 4.601170320470701 5 1.0723722761452112 2.963942304998925 6 0.6420390956362702 2.129447259334304 7 0.4076589816799472 1.5774268135877358 8 0.28503655018400104 1.2605053798640502 9 0.20822336893641374 1.0359198185702043 >10 1.098429472438682 11.292398903048674 >50 0.08919496498074299 3.3594131746908147 >100 0.04068485721044875 3.951695061260564 >500 0.002033478356673606 0.7319234601223977 >1k 0.0013864625159138201 1.2300401660723617 >5k 3.697233375770187E-4 1.409738233035745 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9017 0.4562818067686777 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8101 0.4099300118257799 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5426 0.2745686019215754 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5047 0.2553902937519703 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3061 0.15489393484739075 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2236 0.11314695796104729 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2191 0.1108698501308831 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2016 0.1020144307913557 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 5.060239622587088E-5 0.0 5.060239622587088E-5 0.0 5 0.0 5.060239622587088E-5 0.0 5.060239622587088E-5 0.0 6 0.0 5.060239622587088E-5 0.0 5.060239622587088E-5 0.0 7 1.5180718867761263E-4 5.060239622587088E-5 0.0 5.060239622587088E-5 0.0 8 1.5180718867761263E-4 5.060239622587088E-5 0.0 5.060239622587088E-5 0.0 9 1.5180718867761263E-4 5.060239622587088E-5 0.0 5.060239622587088E-5 0.0 10-11 1.5180718867761263E-4 5.060239622587088E-5 0.0 5.060239622587088E-5 0.0 12-13 3.0361437735522526E-4 5.060239622587088E-5 0.0 5.060239622587088E-5 0.0 14-15 4.0481916980696705E-4 5.060239622587088E-5 0.0 7.590359433880633E-5 0.0 16-17 4.0481916980696705E-4 5.060239622587088E-5 0.0 1.0120479245174176E-4 0.0 18-19 4.554215660328379E-4 5.060239622587088E-5 0.0 1.5180718867761263E-4 0.0 20-21 5.060239622587088E-4 1.5180718867761263E-4 0.0 2.0240958490348352E-4 0.0 22-23 5.566263584845796E-4 1.5180718867761263E-4 0.0 2.783131792422898E-4 0.0 24-25 7.590359433880632E-4 1.5180718867761263E-4 0.0 4.554215660328379E-4 0.0 26-27 7.590359433880632E-4 1.5180718867761263E-4 0.0 8.602407358398051E-4 0.0 28-29 9.361443301786113E-4 1.5180718867761263E-4 0.0 0.0020999994433736416 0.0 30-31 9.614455282915467E-4 1.5180718867761263E-4 0.0 0.006021685150878635 0.0 32-33 9.614455282915467E-4 1.5180718867761263E-4 0.0 0.014193972141356783 0.0 34-35 0.001037349122630353 1.5180718867761263E-4 0.0 0.028210835895923013 0.0 36-37 0.0010626503207432885 1.5180718867761263E-4 0.0 0.05174095014095297 0.0 38-39 0.0010626503207432885 1.5180718867761263E-4 0.0 0.085037326857576 0.0 40-41 0.0010626503207432885 1.5180718867761263E-4 0.0 0.12470960549865878 0.0 42-43 0.0010626503207432885 1.5180718867761263E-4 0.0 0.16344573980956295 0.0 44-45 0.0011385539150820948 1.5180718867761263E-4 0.0 0.21359271446940098 2.530119811293544E-5 46-47 0.001214457509420901 1.5180718867761263E-4 0.0 0.2740372767612037 5.060239622587088E-5 48-49 0.001315662301872643 1.5180718867761263E-4 0.0 0.34123725894916024 5.060239622587088E-5 50-51 0.001315662301872643 1.5180718867761263E-4 0.0 0.41223242085405715 5.060239622587088E-5 52-53 0.001315662301872643 1.5180718867761263E-4 0.0 0.4837842091174385 5.060239622587088E-5 54-55 0.001315662301872643 1.5180718867761263E-4 0.0 0.5599408154373742 5.060239622587088E-5 56-57 0.0014168670943243847 1.5180718867761263E-4 0.0 0.6462432022005971 5.060239622587088E-5 58-59 0.0014168670943243847 2.0240958490348352E-4 0.0 0.7332034201147561 5.060239622587088E-5 60-61 0.0014168670943243847 2.0240958490348352E-4 0.0 0.821023878764755 5.060239622587088E-5 62-63 0.0014168670943243847 2.2771078301641896E-4 0.0 0.9085913254336246 5.060239622587088E-5 64-65 0.0014674694905502555 3.0361437735522526E-4 0.0 1.0007129877628227 5.060239622587088E-5 66-67 0.0015180718867761264 3.0361437735522526E-4 0.0 1.0988816364410119 5.060239622587088E-5 68-69 0.0015686742830019972 3.0361437735522526E-4 0.0 1.2189611226850037 5.060239622587088E-5 70-71 0.0016445778773408037 3.0361437735522526E-4 0.0 1.3849369823058602 5.060239622587088E-5 72-73 0.00172048147167961 3.542167735810962E-4 0.0 1.5626272966530057 5.060239622587088E-5 74-75 0.001771083867905481 3.542167735810962E-4 0.0 1.7526898968773768 5.060239622587088E-5 76-77 0.001771083867905481 3.542167735810962E-4 0.0 1.9695717671014592 5.060239622587088E-5 78-79 0.001771083867905481 3.542167735810962E-4 0.0 2.2201295320138588 5.060239622587088E-5 80-81 0.0019987946509218996 3.542167735810962E-4 0.0 2.5030981317089287 5.060239622587088E-5 82-83 0.002024095849034835 3.542167735810962E-4 0.0 2.780525769017266 5.060239622587088E-5 84-85 0.002074698245260706 3.542167735810962E-4 0.0 3.061217260882172 5.060239622587088E-5 86-87 0.0021759030377124476 3.542167735810962E-4 0.0 3.3466400767941966 5.060239622587088E-5 88-89 0.0022265054339383186 3.542167735810962E-4 0.0 3.6586544519229163 5.060239622587088E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10085 0.0 25.139528 1 TACATGG 10145 0.0 24.660116 2 ACATGGG 10380 0.0 22.954245 3 GAGTACT 8425 0.0 20.550425 12-13 AGTACTT 8795 0.0 20.412994 12-13 CATGGGA 6770 0.0 20.360886 4 GTATCAA 17355 0.0 20.015137 1 GGTATCA 13620 0.0 19.762888 1 CGCATAG 220 0.0 19.7484 66-67 GTACTTT 8900 0.0 19.214123 14-15 CATGGGG 3975 0.0 19.185854 4 CGCGTGA 65 0.006892818 18.566872 66-67 TCGCGCG 65 0.006892818 18.566872 66-67 TAGGACA 970 0.0 18.06851 4 TACTTTT 10025 0.0 17.743076 14-15 AGAGTAC 15560 0.0 17.626753 10-11 TGCACCG 435 3.6379788E-12 17.423006 5 AGTACAT 7880 0.0 17.197016 2 GTGTAGC 1110 0.0 17.077183 1 TACACTG 1915 0.0 17.067608 5 >>END_MODULE