FastQCFastQC Report
Fri 27 May 2016
SRR939456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975143
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64440.32625485850897884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49050.2483364495633987No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33310.16864601702256493No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32550.16479819435858567No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19760.10004338926346093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88400.026.729821
TACATGG89950.025.4504662
GTATCAA156250.024.3820921
GAGTACT71350.023.51714912-13
ACATGGG95200.022.7277263
GTACTTT77900.021.74919914-15
ACGCAGG6800.021.3551527
GGTATCA119750.020.6517931
TATCAAC182200.020.601652
ATCAACG185450.020.5083223
TCAACGC187500.020.3656774
AGTACTT75250.020.35240212-13
CAACGCA186400.020.217555
CATGGGA61900.020.1780074
GTATAGG5000.020.0247141
ATTCGCG2250.019.9703780-81
AACGCAG188850.019.9407586
ACTTTTT99200.019.13902316-17
CATGGGG36750.018.0312184
AGAGTAC136200.017.8755210-11
Produced by FastQC (version 0.11.5)